Building on the recent experimental achievements obtained with scanning electron microscopy on ultracold atoms, we study one-dimensional Bose gases in the crossover between the weakly (quasicondensate) and the strongly interacting (Tonks-Girardeau) regime. We measure the temporal two-particle correlation function and compare it with calculations performed using the time-evolving block decimation algorithm. More pronounced antibunching is observed when entering the more strongly interacting regime. Even though this mimics the onset of a fermionic behavior, we highlight that the exact and simple duality between one-dimensional bosons and fermions does not hold when such a dynamical response is probed. The onset of fermionization is also reflected in the density distribution, which we measure in situ to extract the relevant parameters and to identify the different regimes. Our results show agreement between experiment and theory and give insight into the dynamics of strongly correlated many-body systems.