A novel, low-cost, sensitive microwave microfluidic glucose detecting biosensor incorporating molecularly imprinted polymer (MIP) is presented. The sensing device is based on a stub resonator to characterize water glucose solutions. The tip of one of the stubs is coated with MIP to increase the selectivity of the sensor and hence the sensitivity compared to the uncoated or to the coated with non-imprinted polymer (NIP) sensor. The sensor was fabricated on a FR4 substrate for low-cost purposes. In the presence of the MIP, the sensor loaded with a glucose solution ranging from 50 mg/dL to 400 mg/dL is observed to experience an absorption frequency shift of 73 MHz when the solutions flow in a microfluidic channel passing sensing area, while the lower limit of detection (LLD) of the sensor is discovered to be 2.4 ng/dL. The experimental results show a high sensitivity of 1.3 MHz/(mg/dL) in terms of absorption frequency.