Sensitive detection of Japanese encephalitis virus by surface molecularly imprinted technique based on fluorescent method
W Feng, C Liang, H Gong, C Cai - New Journal of Chemistry, 2018 - pubs.rsc.org
W Feng, C Liang, H Gong, C Cai
New Journal of Chemistry, 2018•pubs.rsc.orgThis study demonstrated fluorescent detection of Japanese encephalitis virus (JEV) by
surface molecularly imprinted technique. After dansyl chloride (DNS-Cl) was immobilized on
silica microspheres (SiMP) with JEV as the template,(3-aminopropyl) triethoxysilane
(APTES) as the monomer and tetraethyl orthosilicate (TEOS) as the cross-linker was mixed
with SiMP, and the polymerization was performed in mild condition. After polymerization,
templates were removed to obtain JEV-imprinted polymers (JEV-MIPs). The obtained JEV …
surface molecularly imprinted technique. After dansyl chloride (DNS-Cl) was immobilized on
silica microspheres (SiMP) with JEV as the template,(3-aminopropyl) triethoxysilane
(APTES) as the monomer and tetraethyl orthosilicate (TEOS) as the cross-linker was mixed
with SiMP, and the polymerization was performed in mild condition. After polymerization,
templates were removed to obtain JEV-imprinted polymers (JEV-MIPs). The obtained JEV …
This study demonstrated fluorescent detection of Japanese encephalitis virus (JEV) by surface molecularly imprinted technique. After dansyl chloride (DNS-Cl) was immobilized on silica microspheres (SiMP) with JEV as the template, (3-aminopropyl)triethoxysilane (APTES) as the monomer and tetraethyl orthosilicate (TEOS) as the cross-linker was mixed with SiMP, and the polymerization was performed in mild condition. After polymerization, templates were removed to obtain JEV-imprinted polymers (JEV-MIPs). The obtained JEV-MIPs can selectively recognize JEV in the presence of Hepatitis A virus (HAV), Simian virus 40 (SV40) and Rabies virus (RV), and also sensitively detect JEV with a picomolar detection limit.
The Royal Society of Chemistry