Surface molecularly imprinted polydopamine films for recognition of immunoglobulin G
A Tretjakov, V Syritski, J Reut, R Boroznjak… - Microchimica Acta, 2013 - Springer
Microchimica Acta, 2013•Springer
We have prepared a surface imprinted polymer (SIP) film for label-free recognition of
immunoglobulin G (IgG). The IgG-SIPs were obtained by covalent immobilization of IgG via a
cleavable covalent bond and a suitable spacer unit to a gold electrode, followed by
electrodepostion of a nm-thin film of polydopamine (PDA). The IgG was then removed by
destruction of the cleavable bond so that complementary binding sites were created on the
surface of the film. IgG-SIPs with various thicknesses of the PDA films were compared with …
immunoglobulin G (IgG). The IgG-SIPs were obtained by covalent immobilization of IgG via a
cleavable covalent bond and a suitable spacer unit to a gold electrode, followed by
electrodepostion of a nm-thin film of polydopamine (PDA). The IgG was then removed by
destruction of the cleavable bond so that complementary binding sites were created on the
surface of the film. IgG-SIPs with various thicknesses of the PDA films were compared with …
Abstract
We have prepared a surface imprinted polymer (SIP) film for label-free recognition of immunoglobulin G (IgG). The IgG-SIPs were obtained by covalent immobilization of IgG via a cleavable covalent bond and a suitable spacer unit to a gold electrode, followed by electrodepostion of a nm-thin film of polydopamine (PDA). The IgG was then removed by destruction of the cleavable bond so that complementary binding sites were created on the surface of the film. IgG-SIPs with various thicknesses of the PDA films were compared with respect to their affinity to IgG using a quartz crystal microbalance combined with flow injection analysis. The films were also characterized by cyclic voltammetry and scanning electron microscopy. The IgG-SIPs with a film thickness of around 17 nm showed the most pronounced imprinting effect (IF 1.66) and a binding constant of 296 nM.
Figure
A strategy for preparation of the IgG-Surface Imprinted Polymeric (IgG-SIP) thin films was developed. IgG was covalently immobilized via a cleavable cross-linker to a gold electrode surface followed by electrochemical deposition of a nanometer thin PDA film. After cleaving S-S bond in the linker the IgG was removed leaving behind the complementary binding sites confined in the surface of the polymer film. The prepared IgG-SIPs were applied for IgG recognition.
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