Teaching, Hospital service, and clinical Research in Biochemistry and related area.
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1. Research Gate: https://www.researchgate.net/profile/Vinod_Bhagwat/
2. I am Scientist: http://www.iamscientist.com/people/sarus1 Phone: +912562239407
Iodine deficiency disorders (IDD) are prevalent in all states of India. IDD continue to be high d... more Iodine deficiency disorders (IDD) are prevalent in all states of India. IDD continue to be high despite ban on the sale of non-iodised salt. In order to assess the effectiveness of salt iodization programme, a study was undertaken to analyse the iodine content of salt samples sold in Ujjain (M.P.). 108 salt samples, commercially available to general public, were collected at retailer level from urban areas of Ujjain. Iodine content of the samples was estimated using standard iodometric titration method. The results indicated that iodine content in 53.7% of the samples was inadequate. Iodine content was significantly lower in 94.7% of non-branded and non-packed with low cost categories. Therefore, economically poor public is at higher risk of iodine deficiency.
Cement used in building and various construction works is a complex mixture of several metal oxid... more Cement used in building and various construction works is a complex mixture of several metal oxides, silicates and miscellaneous compounds. In India, laborers involved in construction work are constantly exposed to the cement various ways. Biochemical effects of the occupational exposure in 31 workers were assessed by Aspartate transaminase, Alanine transaminase, Calcium, Magnesium, Maolondialdehyde and Ascorbate levels in serum. It was found that serum ALT, AST and calcium levels were elevated as compared to the controls. The Dirties ratio was significantly higher in the workers. The lipid peroxidation index was higher while ascorbate level was significantly lower in the cases. It is concluded that the construction worker show marginal alteration in liver functions which is secondary to oxidative stress generated by complex interaction of toxic cement compounds.
Abstract: The magnitude of occupational lead exposure and its impact on liver functions was asses... more Abstract: The magnitude of occupational lead exposure and its impact on liver functions was assessed in battery manufacture workers around Kolhapur (Maharashtra). Blood lead, urine lead, erythrocyte zinc protoporphyrin, urine -amino laevulinic acid and porphobilinogen ...
Iodine deficiency disorders (IDD) are prevalent in all states of India. IDD continue to be high d... more Iodine deficiency disorders (IDD) are prevalent in all states of India. IDD continue to be high despite ban on the sale of non-iodised salt. In order to assess the effectiveness of salt iodization programme, a study was undertaken to analyse the iodine content of salt samples sold in Ujjain (M.P.). 108 salt samples, commercially available to general public, were collected at retailer level from urban areas of Ujjain. Iodine content of the samples was estimated using standard iodometric titration method. The results indicated that iodine content in 53.7% of the samples was inadequate. Iodine content was significantly lower in 94.7% of non-branded and non-packed with low cost categories. Therefore, economically poor public is at higher risk of iodine deficiency.
Cement used in building and various construction works is a complex mixture of several metal oxid... more Cement used in building and various construction works is a complex mixture of several metal oxides, silicates and miscellaneous compounds. In India, laborers involved in construction work are constantly exposed to the cement various ways. Biochemical effects of the occupational exposure in 31 workers were assessed by Aspartate transaminase, Alanine transaminase, Calcium, Magnesium, Maolondialdehyde and Ascorbate levels in serum. It was found that serum ALT, AST and calcium levels were elevated as compared to the controls. The Dirties ratio was significantly higher in the workers. The lipid peroxidation index was higher while ascorbate level was significantly lower in the cases. It is concluded that the construction worker show marginal alteration in liver functions which is secondary to oxidative stress generated by complex interaction of toxic cement compounds.
Abstract: The magnitude of occupational lead exposure and its impact on liver functions was asses... more Abstract: The magnitude of occupational lead exposure and its impact on liver functions was assessed in battery manufacture workers around Kolhapur (Maharashtra). Blood lead, urine lead, erythrocyte zinc protoporphyrin, urine -amino laevulinic acid and porphobilinogen ...
It is well known that Oxygen free radicals are involved in tissue damage following cutaneous ther... more It is well known that Oxygen free radicals are involved in tissue damage following cutaneous thermal injury. Excessive activity of free radicals at the site of burn injury play significant role in developing complications such as hypertrophic scar formation and multiple organ dysfunction syndrome. Thiobarbituric acid reactive substances (TBARS) were measured as marker of lipid peroxidations mediated by free radicals in 40 patients with burn injury ranging from 10 - 60 percent TBSA. Depending on the treatment, two groups were formed. Group (n=2) received topical silver sulphadiazine while Group ll (n=18) received topical honey therapy. Serum levels of uric acid and ceruloplasmin were determined on 7th, 14th, and 21st day after hospitalisation. lt was observed that TBARS level decreased at much rapid rate during honey therapy as compared to silver sulphadiazine treatment. There was mild increment in serum ceruloplasmin level while no significant change was observed in mean uric acid level even up to 21st day. lt appears that topical honey therapy dampens the activity of free radicals in burn trauma. The implications of the findings will be discussed.
Thermal injury to skin results in distinct morphological changes as well as alterations in biomol... more Thermal injury to skin results in distinct morphological changes as well as alterations in biomolecules. These changes lead to tissue damage. This study examines relationship of burn injury and plasma levels of ceruloplasmin, erythrocyte catalase activity and conjugated dienes in 54 patients with thermal injuries ranging from 5 - 92 percent TBSA. 25 normal healthy volunteers served as controls for comparison. The mean plasma ceruloplasmin was significantly increased as compared to erythrocyte catalase in the burn patients. There was a positive correlation between burn size and plasma ceruloplasmin. The implications of the findings will be discussed.
Oxygen free radicals play significant role in tissue damage following cutaneous thermal injury. l... more Oxygen free radicals play significant role in tissue damage following cutaneous thermal injury. ln the present study, serum levels of ascorbic acid, uric acid and lipid peroxidation product was estimated in 54 patients with burn injuries. The burn injury varied from 5 to 92 percent TBSA. 25 healthy volunteers served as controls. The results indicate that serum ascorbic acid level in the patients was significantly low while lipid peroxidation product was much higher than the controls. There was strong positive correlation between the burn size and LPP levels whereas ascorbic acid levels were negatively correlated with the burn injury.
Radiation therapy with cobalt-60 is one of the mainline treatments for solid tumors. Cervical car... more Radiation therapy with cobalt-60 is one of the mainline treatments for solid tumors. Cervical carcinoma patients ideally receive radiotherapy for maximum clinical benefits. The present study was carried out in 62 patients with cervical carcinoma at various clinical stages. Malondialdehyde was measured colorimetrically as the biochemical marker of free radical mediated lipid peroxidations. Major cellular antioxidant enzymes like superoxide dismutase and glutathione peroxidase were measured in the sera of the patients before and 7 days after the radiotherapy protocol. The values were compared with 50 normal healthy controls. The results showed that a maximum decrease in the enzymes levels was seen in stage IV than in stage. There is a linear relationship of the antioxidant enzyme levels with the clinical stages in response to radiotherapy.
ln human malaria, clinical symptomatology differs according to parasite infestation. A comparison... more ln human malaria, clinical symptomatology differs according to parasite infestation. A comparison in the biochemical variables, used to assess iron homeostasis, was made in malarial patients. Biochemical parameters such as serum iron, TIBC, transferrin, ceruloplasmin and lipid peroxidation product together with hematological parameters were measured in 21 patients with P. vivax and 14 patients with P. falciparum malaria along with 25 appropriate normal healthy controls. lt was found that the mean levels of serum iron, TIBC, transferrin saturation and lipid peroxidation product were lower, whereas, mean level of ceruloplasmin was higher in P. vivax than P. falciparum malaria. Results indicate that iron homeostasis is altered in malaria. The changes were more pronounced and severe in falciparum than vivax malaria. This finding has important implications in the clinical management of malarial patients.
A series of changes occur following burn injury. These involve not only morphological changes in ... more A series of changes occur following burn injury. These involve not only morphological changes in tissues but also alteration in biomolecules including biochemical reactions. Activity of free radicals increase tremendously at the site of thermal injury. Serum lipid peroxides, ceruloplasmin and uric acid levels were measured in 60 burn patients receiving topical treatments with honey and silver sulphadiazine. It is observed that serum lipid peroxides fall at much rapid rate in honey treatment than with silver sulphadiazine treatment. There was mild increment in ceruloplasmin levels while no significant change was seen in mean serum uric acid level. It appears that honey treatment dampens the activity of free radicals in burn trauma. The implications of the results will be discussed.
The paper reports on the use of a sensitive and reproducible method for monitoring anti-epileptic... more The paper reports on the use of a sensitive and reproducible method for monitoring anti-epileptic drugs (AED) in the cases of mental retardation (MR) complicated by epilepsy. The AEDs diphenylhydantion and Phenobarbitone were assayed using the method enzyme multiplied immunoassay technique (EMIT). 25 MR children with epilepsy were examined and for comparison, age-sex matched 25 children with epilepsy only were selected. It was considered necessary to use a very sensitive technique for AED assay in MR children with epilepsy, since it is difficult to evaluate side effects in an objective manner. The observations made would be reported and the findings discussed.
Studies were made to determine the relative merits and demerits of various procedures for anti ep... more Studies were made to determine the relative merits and demerits of various procedures for anti epileptic drug (AED) assay in body fluids. The methods used were Colorimetry, UV-Spectrophotometry and enzyme multiplied immunoassay technique (EMIT). About 150 samples were simultaneously processed using the various techniques in determining diphenylhydantoin and phenobarbitone. In order of merit, the following were noted viz., Emit > UV method > colorimetry. However if one considers the cost-benefit aspect the order would be exactly reverse. But yet, in certain situations, despite the cost, Emit was found to be superior and extremely utilitarian, the details of which will be discussed.
An analysis was made of a battery of enzymes in CSF samples obtained from patients with different... more An analysis was made of a battery of enzymes in CSF samples obtained from patients with different neuropsychiatric disorders. Spectrophotometric methods were used for the following enzymes - lactic dehydrogenase (LDH), malic dehydrogenase (MDH), phosphohexoseisomerase (PHI), transaminase (OT & PT), creatine phosphokinase (CPK) and aldolase (ALD). In fluids obtained from patients with CNS infections, LDH and PHI were markedly increased which showed a significant reduction after suitable therapy and remission. The changes in OT and PT paralleled somewhat the changes in LDH and PHI. A significant increase in LDH, PHI and CPK was observed in cases with brain tremors. The utility of CSF enzyme assay in diagnosis of CNS disorders will be discussed.
The protein content of CSF under normal circumstances is less than 50 mg% and hence the method em... more The protein content of CSF under normal circumstances is less than 50 mg% and hence the method employed must be fairly sensitive. The present studies report observations made during the use of three different techniques simultaneously for the assay of protein in 250 patients with different neuropsychiatric disorders. The comparative assessment was made of the turbidimetric methods (using trichloroacetic acid and perchloric acid), the colorimetric method
(using Folin's reagent) and the dye binding method (using Coomassie Brilliant Blue). It was found that the turbidimetric methods are unreliable unless optimal temperature is maintained. The colorimetric method was found to be affected by medication. The present studies indicate that the dye binding method is sensitive and reproducible for different ranges of protein values and can be used routinely for the determination of proteins in CSF.
Asian Waterfowl Census-1990; Perennou, C., Rose, P. & Poole, C. Mid-Winter waterfowl counts at Ma... more Asian Waterfowl Census-1990; Perennou, C., Rose, P. & Poole, C. Mid-Winter waterfowl counts at Mayani and Pingali water reservoirs, District Satara (India) in Jan 1990. Census organised by International Waterfowl and Wetland Bureau, UK and Wetland International, Malaysia.
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Papers by V. R. Bhagwat
(using Folin's reagent) and the dye binding method (using Coomassie Brilliant Blue). It was found that the turbidimetric methods are unreliable unless optimal temperature is maintained. The colorimetric method was found to be affected by medication. The present studies indicate that the dye binding method is sensitive and reproducible for different ranges of protein values and can be used routinely for the determination of proteins in CSF.