DNK giraza
| DNK giraza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
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| Dimer DNK topoizomeraze IIA (čovjek) | |||||||||
| Identifikatori | |||||||||
| EC broj | 5.99.1.3 | ||||||||
| CAS broj | 142805-56-9 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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DNK giraza je enzim koji umanjuje naprezanje tokom odvijanja dvolančane DNK helikazom. To dovodi do negativnog supernamotavanja DNK. Bakterijska DNK giraza je biološka meta mnogih antibiotika, neki od kojih su nalidiksilna kiselina, novobiocin, i ciprofloksacin.
DNK giraza je tip II topoizomeraza (EC 5.99.1.3) koja uvodi negativne supernamotaje (ili relaksira pozitivne supernamotaje) u DNK. Ona savija templet tako da se formira ukrštanje, zatim preseca jedan od dvostrukih heliksa i propušta drugi kroz taj otvor. Taj proces se odvija kod prokariota (posebno kod bakterija). Nedavno je giraza je nađena kod apikoplast malerijskog parazita Plasmodium falciparum, jednoćelijskog eukariota.
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