Additional file 12: Table S8. Pathogenesis-related (PR) gene expression in WT-Az and MIR7695-Ac p... more Additional file 12: Table S8. Pathogenesis-related (PR) gene expression in WT-Az and MIR7695-Ac plants during M. oryzae infection at 48 hpi.
Additional file 1: Figure S1. Characterization and phenotype of MIR7695-Ac and wild-type azygous ... more Additional file 1: Figure S1. Characterization and phenotype of MIR7695-Ac and wild-type azygous (WT-Az) plants.
Additional file 15: Figure S5. Pathways for the biosynthesis of phenylpropanoids and diterpenoid ... more Additional file 15: Figure S5. Pathways for the biosynthesis of phenylpropanoids and diterpenoid phytoalexins in rice.
Additional file 16: Figure S6. RT-qPCR analysis of expression pattern of Fe homeostasis genes in ... more Additional file 16: Figure S6. RT-qPCR analysis of expression pattern of Fe homeostasis genes in WT-Az and MIR7695-Ac plants with M. oryzae infection.
Background The early-ripening rice races are important because they may escape droughts, avoid fl... more Background The early-ripening rice races are important because they may escape droughts, avoid floods and reduce the growing period. Thus, they could be cultivated for at least two cropping seasons each year in sub-tropical or tropical areas. According to Song Dynasty history, many rice accessions were brought to China from the Champa Kingdom, and these lines were drought-resistant and non-photoperiod–sensitive. Here, we address the impact of Champa rice on rice production, and which subtype the Champa rice belong to.Results We performed a phylogenetic analysis of rice landraces in China and Taiwan that had the word “Champa” in their names. We also cooperated with rice breeders in Vietnam, where the ancient Champa Kingdom was. Together with the publicly available genome sequencing data, we confirmed that many Taiwan landraces are also Champa rice. We also showed that these Champa rice played important roles in ancient and modern rice production. The most famous landrace is Dee Geo W...
Background MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post... more Background MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level in eukaryotes. In rice, MIR7695 expression is regulated by infection with the rice blast fungus Magnaporthe oryzae with subsequent down-regulation of an alternatively spliced transcript of natural resistance-associated macrophage protein 6 (OsNramp6). NRAMP6 functions as an iron transporter in rice. Results Rice plants grown under high iron supply showed blast resistance, which supports that iron is a factor in controlling blast resistance. During pathogen infection, iron accumulated in the vicinity of M. oryzae appressoria, the sites of pathogen entry, and in cells surrounding infected regions of the rice leaf. Activation-tagged MIR7695 rice plants (MIR7695-Ac) exhibited enhanced iron accumulation and resistance to M. oryzae infection. RNA-seq analysis revealed that blast resistance in MIR7695-Ac plants was associated with strong induction of defense-related genes...
Rice (Oryza sativa) is one of the most important crops in the world. Several rice insertional mut... more Rice (Oryza sativa) is one of the most important crops in the world. Several rice insertional mutant libraries are publicly available for systematic analysis of gene functions. However, the tagging efficiency of these mutant resources-the relationship between genotype and phenotype-is very low. We used whole-genome sequencing to analyze a T-DNA-tagged transformant from the Taiwan Rice Insertional Mutants (TRIM) resource. The phenomics records for M0028590, one of the TRIM lines, revealed three phenotypes-wild type, large grains, and tillering dwarf-in the 12 T1 plants. Using the sequencing data for 7 plants from three generations of this specific line, we demonstrate that introgression from an indica rice variety might occur in one generation before the seed was used for callus generation and transformation of this line. In addition, the large-grain trait came from the GS3 gene of the introgressed region and the tillering dwarf phenotype came from a single nucleotide change in the D...
2011 IEEE 11th International Conference on Bioinformatics and Bioengineering, 2011
Abstract For the purpose of promoting the utilization of T-DNA mutant seeds generated by the Taiw... more Abstract For the purpose of promoting the utilization of T-DNA mutant seeds generated by the Taiwan Rice Insertional Mutagenesis (TRIM) program for academic research, Academia Sinica contracted Asia University to establish a T-DNA Tagged Rice Service Center ( ...
Additional file 12: Table S8. Pathogenesis-related (PR) gene expression in WT-Az and MIR7695-Ac p... more Additional file 12: Table S8. Pathogenesis-related (PR) gene expression in WT-Az and MIR7695-Ac plants during M. oryzae infection at 48 hpi.
Additional file 1: Figure S1. Characterization and phenotype of MIR7695-Ac and wild-type azygous ... more Additional file 1: Figure S1. Characterization and phenotype of MIR7695-Ac and wild-type azygous (WT-Az) plants.
Additional file 15: Figure S5. Pathways for the biosynthesis of phenylpropanoids and diterpenoid ... more Additional file 15: Figure S5. Pathways for the biosynthesis of phenylpropanoids and diterpenoid phytoalexins in rice.
Additional file 16: Figure S6. RT-qPCR analysis of expression pattern of Fe homeostasis genes in ... more Additional file 16: Figure S6. RT-qPCR analysis of expression pattern of Fe homeostasis genes in WT-Az and MIR7695-Ac plants with M. oryzae infection.
Background The early-ripening rice races are important because they may escape droughts, avoid fl... more Background The early-ripening rice races are important because they may escape droughts, avoid floods and reduce the growing period. Thus, they could be cultivated for at least two cropping seasons each year in sub-tropical or tropical areas. According to Song Dynasty history, many rice accessions were brought to China from the Champa Kingdom, and these lines were drought-resistant and non-photoperiod–sensitive. Here, we address the impact of Champa rice on rice production, and which subtype the Champa rice belong to.Results We performed a phylogenetic analysis of rice landraces in China and Taiwan that had the word “Champa” in their names. We also cooperated with rice breeders in Vietnam, where the ancient Champa Kingdom was. Together with the publicly available genome sequencing data, we confirmed that many Taiwan landraces are also Champa rice. We also showed that these Champa rice played important roles in ancient and modern rice production. The most famous landrace is Dee Geo W...
Background MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post... more Background MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level in eukaryotes. In rice, MIR7695 expression is regulated by infection with the rice blast fungus Magnaporthe oryzae with subsequent down-regulation of an alternatively spliced transcript of natural resistance-associated macrophage protein 6 (OsNramp6). NRAMP6 functions as an iron transporter in rice. Results Rice plants grown under high iron supply showed blast resistance, which supports that iron is a factor in controlling blast resistance. During pathogen infection, iron accumulated in the vicinity of M. oryzae appressoria, the sites of pathogen entry, and in cells surrounding infected regions of the rice leaf. Activation-tagged MIR7695 rice plants (MIR7695-Ac) exhibited enhanced iron accumulation and resistance to M. oryzae infection. RNA-seq analysis revealed that blast resistance in MIR7695-Ac plants was associated with strong induction of defense-related genes...
Rice (Oryza sativa) is one of the most important crops in the world. Several rice insertional mut... more Rice (Oryza sativa) is one of the most important crops in the world. Several rice insertional mutant libraries are publicly available for systematic analysis of gene functions. However, the tagging efficiency of these mutant resources-the relationship between genotype and phenotype-is very low. We used whole-genome sequencing to analyze a T-DNA-tagged transformant from the Taiwan Rice Insertional Mutants (TRIM) resource. The phenomics records for M0028590, one of the TRIM lines, revealed three phenotypes-wild type, large grains, and tillering dwarf-in the 12 T1 plants. Using the sequencing data for 7 plants from three generations of this specific line, we demonstrate that introgression from an indica rice variety might occur in one generation before the seed was used for callus generation and transformation of this line. In addition, the large-grain trait came from the GS3 gene of the introgressed region and the tillering dwarf phenotype came from a single nucleotide change in the D...
2011 IEEE 11th International Conference on Bioinformatics and Bioengineering, 2011
Abstract For the purpose of promoting the utilization of T-DNA mutant seeds generated by the Taiw... more Abstract For the purpose of promoting the utilization of T-DNA mutant seeds generated by the Taiwan Rice Insertional Mutagenesis (TRIM) program for academic research, Academia Sinica contracted Asia University to establish a T-DNA Tagged Rice Service Center ( ...
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