I've been educated in Microbiology & Molecular Microbiology. Now I've been work on Resistant mechanisms of Microorganisms especially Mycobacterium sp., Pseudomonas aeruginosa, Acinetobacter sp. Either another interest of mine is mechanisms of bacterial pathogenicity. Phone: 9173168640 Address: Shiraz Medical School- Block 3- 4th floor-Shiraz-Zand Street-Fars Province-Iran
Toothpastes are considered as one of the most common and usable cosmetic and hygienic materials. ... more Toothpastes are considered as one of the most common and usable cosmetic and hygienic materials. Such materials contain chemicals which may have an adverse effect on oral tissue in humans. The present study aimed to compare the toxic effect of current commercial toothpastes including Iranian products and imported types which are consumed globally on oral epithelial- and HeLa cells as well as to evaluate their antibacterial effect on Streptococcus mutans in Shiraz, Iran. In this experimental study, 16 types of commercial toothpastes were prepared, and their effect was determined on primer epithelial cells of the oral cavity and HeLa cells. Toothpastes anti streptococcal property and toxicity were examined in vitro in different intervals of 1, 2, and 5 min. Data collection and analysis were done using one-way analysis of variance. All experimented toothpastes revealed variable toxic effects on cultured cells. Through an increase in the time of exposure with toothpastes, the toxicity o...
Background The first large serosurvey in Iran found a SARS-CoV-2 antibody seroprevalence of 17.1%... more Background The first large serosurvey in Iran found a SARS-CoV-2 antibody seroprevalence of 17.1% among the general population in the first wave of the epidemic by April, 2020. The purpose of the current study was to assess the seroprevalence of COVID-19 infection among Iranian general population after the third wave of the disease. Methods This population-based cross-sectional study was conducted on 7411 individuals aged ≥10 years old in 16 cities across 15 provinces in Iran between January and March, 2021. We randomly sampled individuals registered in the Iranian electronic health record system based on their national identification numbers and invited them by telephone to a healthcare center for data collection. Presence of SARS-CoV-2-specific IgG and IgM antibodies was assessed using the SARS-CoV-2 ELISA kits. The participants were also asked about their recent COVID-19-related symptoms, including cough, fever, chills, sore throat, headache, dyspnea, diarrhea, anosmia, conjuncti...
Avicenna Journal of Clinical Microbiology and Infection
Background: The rapid spread of the virus around the world is raising alarms among scientists to ... more Background: The rapid spread of the virus around the world is raising alarms among scientists to identify vulnerable people who are at greater risk of infection. In this regard, the present study aimed to determine the prevalence of severe acute respiratory syndrome coronavirus 2 in different blood groups. Methods: To find relevant studies, a comprehensive and systematic search was conducted based on the PRISMA guidelines in international databases such as PubMed, Scopus, Web of Sciences, and Google Scholar by December 31, 2020. Results: After the audit and exclusion of double and unrelated studies, 19 articles were included in the analysis. The most prevalent blood types in alive patients were A and O which calculated the aggregate prevalence at - 39.06 (95% CI: 36.22-41.94) and 35.60 (95% CI: 32.48-38.79). In addition, patients with blood groups B and AB were less than two other groups. The aggregated/estimated prevalence was 7.72 (95% CI: 5.06-10.88) and 16.23 (95% CI: 12.86-19.9...
Background: Recently, people have recognized the post-acute phase symptoms of the COVID-19. We in... more Background: Recently, people have recognized the post-acute phase symptoms of the COVID-19. We investigated the long-term symptoms associated with COVID-19, (Long COVID Syndrome), and the risk factors associated with it. Methods: This was a retrospective observational study. All the consecutive adult patients referred to the healthcare facilities anywhere in Fars province from 19 February 2020 until 20 November 2020 were included. All the patients had a confirmed COVID-19 diagnosis. In a phone call to the patients, at least three months after their discharge from the hospital, we obtained their current information. The IBM SPSS Statistics (version 25.0) was used. Pearson Chi square, Fisher’s exact test, t test, and binary logistic regression analysis model were employed. A P value of less than 0.05 was considered to be significant Results: In total, 4,681 patients were studied, 2915 of whom (62.3%) reported symptoms. The most common symptoms of long COVID syndrome were fatigue, exer...
Background: The pandemic and regional influenza outbreaks resulting from antigenic variation of i... more Background: The pandemic and regional influenza outbreaks resulting from antigenic variation of influenza viruses have been the subject of numerous studies which are crucial to the preparation of the vaccine. Frequent global winter outbreaks of influenza viruses require a constant surveillance of emerging influenza variants in order to develop efficient influenza vaccine. Methods: This study was conducted from December 2004 to March 2005, during an outbreak of influenza in 300 children of one to 15-year-old. MDCK cells grown in microwell plates inoculated with pharyngeal swabs. Hemagglutination (HA) of guinea pig erythrocytes by viral isolates was used for initial screening of influenza viruses. The final viral identification was performed by HA inhibition test using reference antisera prepared against WHO reference antigens. Results: Based on the results of HA tests with culture fluid of MDCK inoculated with 300 pharyngeal swabs, 31 (10.3%) of the specimens were suspected to conta...
The Journal of Qazvin University of Medical Sciences, 2016
Abstract Background: Drug resistance is one of the important threats in uncontrolled infections ... more Abstract Background: Drug resistance is one of the important threats in uncontrolled infections by Pseudomonas aeruginosa, an opportunistic nosocomial pathogen, in burn patients. The presence of OprD porin protein in the bacterial cell wall is one of the mechanisms for resistance against hydrophilic drugs in this bacterium. Objective: The aim of this study was to evaluate genetic sequence rearrangements of OprD gene in imipenem resistant clinical isolates of pseudomonas aeruginosa in burn patients. Methods: This cross sectional study was performed in Ghotbeddin Shirazi Hospital from October 2013 to February 2015. A total of 253 wound samples were evaluated for Pseudomonas aeruginosa. All isolates were evaluated using specific sequencing of the target region. Genetic sequence rearrangements were compared with the sensitivity pattern of the isolates to the imipenem. Findings: Pseudomonas aeruginosa was found in 22% of the samples in Shiraz burn center. More than 90% of the isolates w...
International Journal of Molecular and Clinical Microbiology, 2017
ARTICLE INFO ABSTRACT Article history: Received 4 January 2017 Accepted 19 April 2017 Available o... more ARTICLE INFO ABSTRACT Article history: Received 4 January 2017 Accepted 19 April 2017 Available online 1 June 2017 Although, many genes are involved in pathogenesis of Campylobacter, racR gene considered the main gene for Campylobacter pathogenicity in humans.The purpose of this study was determined the prevalence of recR gene in Campylobacter spp. isolated from domestic animals and water. To perform the study 392 fecal and water samples were collected from poultry (182), cow (141), sheep (25) and goat (16) and water (28). All samples were subjected for isolation of Campylobacter spp. using prêt KB method and the presumptive isolates authenticated by DNA sequencing of 16srRNA genes. Finally, Campylobacter isolates assessed for detection of racR gene. The results obtained indicated that 50 strains of Campylobacter spp. were isolated. High isolate frequency (37/50) was for Poultry and low frequency (2/50) was for sheep and goat. Of All isolates thirty six strains were identified as Ca...
Background: Despite the whole world’s effort for controlling, an ongoing global outbreak of lower... more Background: Despite the whole world’s effort for controlling, an ongoing global outbreak of lower respiratory tract disease, caused by new corona virus; led to a major public health issue. Current study aims to evaluate the characteristics of infected case in Fars, IranMethods: According to the referral of suspicious patients during one month, 2538 samples were evaluated and extracted for Viral RNA nucleic acids by using the Invitrogen ChargeSwitch® Total RNA Cell Kit (Invitrogen Co.). Data were recorded based on the standardized data collection forms shared by WHO and the International Severe Acute Respiratory and Emerging Infection Consortium. Results: By 19 March 2020, 440 admitted patients had been identified with CoVID-19 confirmation. More than 50% of cases were men. Mean age were estimated 48.15 ±18.07. Of all the patients, 30 (6.81%) had a history of travel to Qom city, which was the first infected zone in Iran, also 92(20.90%) had close contact with contaminated or suspecte...
Introduction: Aeromonas have recently emerged as opportunistic pathogens and only a few studies a... more Introduction: Aeromonas have recently emerged as opportunistic pathogens and only a few studies are available regarding the isolation of these bacteria from burn wound infections. This study aimed at isolating Aeromonas as an infrequent cause of infection in this group of immunocompromised patients. Methods: A total of 300 samples were collected from the wounds of burn patients hospitalized in Gotbodin Shirazi Burn Center in 2013. The samples were cultured on Aeromonas specific media and then confirmed using standard biochemical tests and 16S rRNA gene amplification and sequencing. The antibiotic susceptibility of the isolates was determined using the disk diffusion and broth microdilution methods. Results: Biochemical tests demonstrated five presumptive samples of Aeromonas, while, molecular testing confirmed only three. All isolates were resistant to ampicillin-sulbactam, erythromycin, oxacillin, and vancomycin. However, they were susceptible to gentamicin, meropenem, nitrofuranto...
INTRODUCTION Recently; multidrug resistance (MDR) Gram negative bacteria have become a serious co... more INTRODUCTION Recently; multidrug resistance (MDR) Gram negative bacteria have become a serious concern causing infections in hospitalized burn patients. This Meta-analysis was conducted to detect the prevalence of infections caused by MDR Gram negative bacteria in hospitalized burn patients in Iran. METHODS An electronic search was performed by using PubMed, Scopus, and Web of Science and Iranian data bases. Statistical analysis was carrying out by STATA13. According to heterogeneity test, fixed or random effect model was used and publication bias was detected based on Egger test. Of 1292 published articles in initial search, 107 studies were included in this review. RESULTS According to the results; the lowest rate of resistance was seen inA. baumannii and P. aeruginosa against Colistin which evaluated 0.21 (95% CI: 0.02 - 0.49, I2: 97.30) and 0.27(95% CI: 0.00 - 0.82, I2: 99.27%), respectively. Either the highest rate was estimated 0.98 (95% CI: 0.93 - 1.00, I2: 88.28) and 0.87 (95% CI: 0.81 - 0.92, I2: 96.27) against Cefepime. CONCLUSION The prevalence of MDR among Iranian burn patients is very high. Thus; a plenary infectious control program, reduction in use of antibiotic prophylaxis and comprehensive information about the antibiotic resistance pattern is needed, essentially.
Pseudomonas aeruginosa is a mobile bacterium and cylindrical in shape, which grows easily in many... more Pseudomonas aeruginosa is a mobile bacterium and cylindrical in shape, which grows easily in many medium. These bacteria can cause serious infections in burn patients due to damages of the skin as the first line of defense so that was a major factor of deaths in about 77% in these patients in the past 25 years. The aim of this study was to investigate the prevalence of multi-drug resistant P. aeruginosa in patients hospitalized at Qutb al-Din Shirazi with Primer 272. This cross-sectional study was conducted experimentally over a period of one year (March 2013 to March 2014) at Burn Research Center, University of Medical Sciences of Shiraz, Iran. In this study, 200 samples were collected by swab from the burn patients admitted to hospital over a period of one year. It should be noted that all samples were taken in the morning before washing the patients and delivered to the microbiology laboratory. All samples were then evaluated using microbiological phenotypic tests for microscopic...
Toothpastes are considered as one of the most common and usable cosmetic and hygienic materials. ... more Toothpastes are considered as one of the most common and usable cosmetic and hygienic materials. Such materials contain chemicals which may have an adverse effect on oral tissue in humans. The present study aimed to compare the toxic effect of current commercial toothpastes including Iranian products and imported types which are consumed globally on oral epithelial- and HeLa cells as well as to evaluate their antibacterial effect on Streptococcus mutans in Shiraz, Iran. In this experimental study, 16 types of commercial toothpastes were prepared, and their effect was determined on primer epithelial cells of the oral cavity and HeLa cells. Toothpastes anti streptococcal property and toxicity were examined in vitro in different intervals of 1, 2, and 5 min. Data collection and analysis were done using one-way analysis of variance. All experimented toothpastes revealed variable toxic effects on cultured cells. Through an increase in the time of exposure with toothpastes, the toxicity o...
Background The first large serosurvey in Iran found a SARS-CoV-2 antibody seroprevalence of 17.1%... more Background The first large serosurvey in Iran found a SARS-CoV-2 antibody seroprevalence of 17.1% among the general population in the first wave of the epidemic by April, 2020. The purpose of the current study was to assess the seroprevalence of COVID-19 infection among Iranian general population after the third wave of the disease. Methods This population-based cross-sectional study was conducted on 7411 individuals aged ≥10 years old in 16 cities across 15 provinces in Iran between January and March, 2021. We randomly sampled individuals registered in the Iranian electronic health record system based on their national identification numbers and invited them by telephone to a healthcare center for data collection. Presence of SARS-CoV-2-specific IgG and IgM antibodies was assessed using the SARS-CoV-2 ELISA kits. The participants were also asked about their recent COVID-19-related symptoms, including cough, fever, chills, sore throat, headache, dyspnea, diarrhea, anosmia, conjuncti...
Avicenna Journal of Clinical Microbiology and Infection
Background: The rapid spread of the virus around the world is raising alarms among scientists to ... more Background: The rapid spread of the virus around the world is raising alarms among scientists to identify vulnerable people who are at greater risk of infection. In this regard, the present study aimed to determine the prevalence of severe acute respiratory syndrome coronavirus 2 in different blood groups. Methods: To find relevant studies, a comprehensive and systematic search was conducted based on the PRISMA guidelines in international databases such as PubMed, Scopus, Web of Sciences, and Google Scholar by December 31, 2020. Results: After the audit and exclusion of double and unrelated studies, 19 articles were included in the analysis. The most prevalent blood types in alive patients were A and O which calculated the aggregate prevalence at - 39.06 (95% CI: 36.22-41.94) and 35.60 (95% CI: 32.48-38.79). In addition, patients with blood groups B and AB were less than two other groups. The aggregated/estimated prevalence was 7.72 (95% CI: 5.06-10.88) and 16.23 (95% CI: 12.86-19.9...
Background: Recently, people have recognized the post-acute phase symptoms of the COVID-19. We in... more Background: Recently, people have recognized the post-acute phase symptoms of the COVID-19. We investigated the long-term symptoms associated with COVID-19, (Long COVID Syndrome), and the risk factors associated with it. Methods: This was a retrospective observational study. All the consecutive adult patients referred to the healthcare facilities anywhere in Fars province from 19 February 2020 until 20 November 2020 were included. All the patients had a confirmed COVID-19 diagnosis. In a phone call to the patients, at least three months after their discharge from the hospital, we obtained their current information. The IBM SPSS Statistics (version 25.0) was used. Pearson Chi square, Fisher’s exact test, t test, and binary logistic regression analysis model were employed. A P value of less than 0.05 was considered to be significant Results: In total, 4,681 patients were studied, 2915 of whom (62.3%) reported symptoms. The most common symptoms of long COVID syndrome were fatigue, exer...
Background: The pandemic and regional influenza outbreaks resulting from antigenic variation of i... more Background: The pandemic and regional influenza outbreaks resulting from antigenic variation of influenza viruses have been the subject of numerous studies which are crucial to the preparation of the vaccine. Frequent global winter outbreaks of influenza viruses require a constant surveillance of emerging influenza variants in order to develop efficient influenza vaccine. Methods: This study was conducted from December 2004 to March 2005, during an outbreak of influenza in 300 children of one to 15-year-old. MDCK cells grown in microwell plates inoculated with pharyngeal swabs. Hemagglutination (HA) of guinea pig erythrocytes by viral isolates was used for initial screening of influenza viruses. The final viral identification was performed by HA inhibition test using reference antisera prepared against WHO reference antigens. Results: Based on the results of HA tests with culture fluid of MDCK inoculated with 300 pharyngeal swabs, 31 (10.3%) of the specimens were suspected to conta...
The Journal of Qazvin University of Medical Sciences, 2016
Abstract Background: Drug resistance is one of the important threats in uncontrolled infections ... more Abstract Background: Drug resistance is one of the important threats in uncontrolled infections by Pseudomonas aeruginosa, an opportunistic nosocomial pathogen, in burn patients. The presence of OprD porin protein in the bacterial cell wall is one of the mechanisms for resistance against hydrophilic drugs in this bacterium. Objective: The aim of this study was to evaluate genetic sequence rearrangements of OprD gene in imipenem resistant clinical isolates of pseudomonas aeruginosa in burn patients. Methods: This cross sectional study was performed in Ghotbeddin Shirazi Hospital from October 2013 to February 2015. A total of 253 wound samples were evaluated for Pseudomonas aeruginosa. All isolates were evaluated using specific sequencing of the target region. Genetic sequence rearrangements were compared with the sensitivity pattern of the isolates to the imipenem. Findings: Pseudomonas aeruginosa was found in 22% of the samples in Shiraz burn center. More than 90% of the isolates w...
International Journal of Molecular and Clinical Microbiology, 2017
ARTICLE INFO ABSTRACT Article history: Received 4 January 2017 Accepted 19 April 2017 Available o... more ARTICLE INFO ABSTRACT Article history: Received 4 January 2017 Accepted 19 April 2017 Available online 1 June 2017 Although, many genes are involved in pathogenesis of Campylobacter, racR gene considered the main gene for Campylobacter pathogenicity in humans.The purpose of this study was determined the prevalence of recR gene in Campylobacter spp. isolated from domestic animals and water. To perform the study 392 fecal and water samples were collected from poultry (182), cow (141), sheep (25) and goat (16) and water (28). All samples were subjected for isolation of Campylobacter spp. using prêt KB method and the presumptive isolates authenticated by DNA sequencing of 16srRNA genes. Finally, Campylobacter isolates assessed for detection of racR gene. The results obtained indicated that 50 strains of Campylobacter spp. were isolated. High isolate frequency (37/50) was for Poultry and low frequency (2/50) was for sheep and goat. Of All isolates thirty six strains were identified as Ca...
Background: Despite the whole world’s effort for controlling, an ongoing global outbreak of lower... more Background: Despite the whole world’s effort for controlling, an ongoing global outbreak of lower respiratory tract disease, caused by new corona virus; led to a major public health issue. Current study aims to evaluate the characteristics of infected case in Fars, IranMethods: According to the referral of suspicious patients during one month, 2538 samples were evaluated and extracted for Viral RNA nucleic acids by using the Invitrogen ChargeSwitch® Total RNA Cell Kit (Invitrogen Co.). Data were recorded based on the standardized data collection forms shared by WHO and the International Severe Acute Respiratory and Emerging Infection Consortium. Results: By 19 March 2020, 440 admitted patients had been identified with CoVID-19 confirmation. More than 50% of cases were men. Mean age were estimated 48.15 ±18.07. Of all the patients, 30 (6.81%) had a history of travel to Qom city, which was the first infected zone in Iran, also 92(20.90%) had close contact with contaminated or suspecte...
Introduction: Aeromonas have recently emerged as opportunistic pathogens and only a few studies a... more Introduction: Aeromonas have recently emerged as opportunistic pathogens and only a few studies are available regarding the isolation of these bacteria from burn wound infections. This study aimed at isolating Aeromonas as an infrequent cause of infection in this group of immunocompromised patients. Methods: A total of 300 samples were collected from the wounds of burn patients hospitalized in Gotbodin Shirazi Burn Center in 2013. The samples were cultured on Aeromonas specific media and then confirmed using standard biochemical tests and 16S rRNA gene amplification and sequencing. The antibiotic susceptibility of the isolates was determined using the disk diffusion and broth microdilution methods. Results: Biochemical tests demonstrated five presumptive samples of Aeromonas, while, molecular testing confirmed only three. All isolates were resistant to ampicillin-sulbactam, erythromycin, oxacillin, and vancomycin. However, they were susceptible to gentamicin, meropenem, nitrofuranto...
INTRODUCTION Recently; multidrug resistance (MDR) Gram negative bacteria have become a serious co... more INTRODUCTION Recently; multidrug resistance (MDR) Gram negative bacteria have become a serious concern causing infections in hospitalized burn patients. This Meta-analysis was conducted to detect the prevalence of infections caused by MDR Gram negative bacteria in hospitalized burn patients in Iran. METHODS An electronic search was performed by using PubMed, Scopus, and Web of Science and Iranian data bases. Statistical analysis was carrying out by STATA13. According to heterogeneity test, fixed or random effect model was used and publication bias was detected based on Egger test. Of 1292 published articles in initial search, 107 studies were included in this review. RESULTS According to the results; the lowest rate of resistance was seen inA. baumannii and P. aeruginosa against Colistin which evaluated 0.21 (95% CI: 0.02 - 0.49, I2: 97.30) and 0.27(95% CI: 0.00 - 0.82, I2: 99.27%), respectively. Either the highest rate was estimated 0.98 (95% CI: 0.93 - 1.00, I2: 88.28) and 0.87 (95% CI: 0.81 - 0.92, I2: 96.27) against Cefepime. CONCLUSION The prevalence of MDR among Iranian burn patients is very high. Thus; a plenary infectious control program, reduction in use of antibiotic prophylaxis and comprehensive information about the antibiotic resistance pattern is needed, essentially.
Pseudomonas aeruginosa is a mobile bacterium and cylindrical in shape, which grows easily in many... more Pseudomonas aeruginosa is a mobile bacterium and cylindrical in shape, which grows easily in many medium. These bacteria can cause serious infections in burn patients due to damages of the skin as the first line of defense so that was a major factor of deaths in about 77% in these patients in the past 25 years. The aim of this study was to investigate the prevalence of multi-drug resistant P. aeruginosa in patients hospitalized at Qutb al-Din Shirazi with Primer 272. This cross-sectional study was conducted experimentally over a period of one year (March 2013 to March 2014) at Burn Research Center, University of Medical Sciences of Shiraz, Iran. In this study, 200 samples were collected by swab from the burn patients admitted to hospital over a period of one year. It should be noted that all samples were taken in the morning before washing the patients and delivered to the microbiology laboratory. All samples were then evaluated using microbiological phenotypic tests for microscopic...
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