To assess continuous recording of the microscope status for quality assurance. Using a special mi... more To assess continuous recording of the microscope status for quality assurance. Using a special microscope with coordinate registration and objective decoding, 8,653 cervical smears were read by five experienced cytotechnologists. Cytotechnologists could check their screening pattern on a TV monitor. Stage position and magnification were registered every 20 msec. The average screening time per case was 3.5 minutes followed by an approximately two-minute intercase interval. Daily workload profiles were generated to check compliance with workload regulations. Average screening time over a day and over a week was rather constant. Our experience demonstrates that continuous coordinate registration can be easily implemented as a method of quality assurance in routine cytology.
Andrea Abati Fadi W. Abdul-Karim Pedro P. de Agustín Måns Åkerman M. Loredana Gadda Alasio Patric... more Andrea Abati Fadi W. Abdul-Karim Pedro P. de Agustín Måns Åkerman M. Loredana Gadda Alasio Patricia Alonso de Ruiz Carmen Alvarez-Santin Ana-Cristina Anton Karen M. Atkison Silvana Audy-Jurkovic Manon Auger R. Marshall Austin Jan P. A. Baak Ulrik Baandrup Gunter F. Bahr Peter H. Bartels Sandra H. Bigner John W. Bishop Alfred Böcking Miklós Bodó Vladimir N. Bogatyrev Thomas A. Bonfiglio Mathilde E. Boon Gerhard Breitenecker Ursula Buerzele-Fricke Jorge Campos R. de C. Carol A. Carriere Patricia Chapman William N. Christopherson Edith Claros Mercado Luiz Martins Collaco Brian T. Collins William N. Crabtree Peter Dalquen Dilip K. Das Diane D. Davey Irma DeLeón-Rodriguez Pranab Dey Manfred Droese Antonio Ducrot-Schinini Jaroslava Duskova S. Hamilton Dutoit Lars Egevad Hormoz Ehya Yener S. Erozan Dorothea Ferlin Clarice Amaral Ferreira Norman W. Fitzgerald Brendan T. Fitzpatrick Alessandra M. Forni Hugo Galera-Davidson Paolo Gattuso Kim R. Geisinger Claude Gompel Ricardo González-Cámpora Zenon Gonzalez-Romero Roberta M. Goodell Philippe de Graeve Shirley E. Greening Heinz K. Grohs Prabodh K. Gupta Raj K. Gupta Steven I. Hajdu Xhevdet Harasani Ernst Held Manuel Hilgarth Anders Hjerpe J. Heinrich Holzner O. A. N. Husain Martha L. Hutchinson Hideo Ikeda Lucrecia Illescas Stanley L. Inhorn Gita Jayaram Matías Jiménez-Ayala William W. Johnston David B. Kaminsky Kusum Kapila Harubumi Kato Ruth L. Katz William H. Kern Tadao K. Kobayashi Reviewers and Consultants
EUS-guided FNA (EUS-FNA) is an established tissue-acquisition technique, with most studies concen... more EUS-guided FNA (EUS-FNA) is an established tissue-acquisition technique, with most studies concentrating on cytologic analyses of specimens, with only few data existing on histologic assessment. To assess the sensitivity of a combined analysis of histologic followed by cytologic tissue diagnosis. A retrospective 3-center study. In consecutive patients undergoing FNA of solid pancreatic masses, core specimens were harvested for histology; residual tissue was examined cytologically. Only unequivocally positive results were regarded as malignant. Criterion standards were positive results from EUS-FNA or other histologic findings, or, if negative, clinical follow-up data (minimum 12 months). Among 192 patients (110 men; mean age 63 years) with mostly pancreatic-head masses (72.4%), overall, adequate tissue was obtained in 98.9% of all cases, with a mean of 1.88 needle passes and an overall sensitivity of 82.9% (95% CI, 76.0%-88.5%). Histology and subsequent cytology provided adequate tissue and sensitivities of 86.5% and 60%, and 92.7% and 68.1%, respectively. Excluding cases with inadequate specimens, sensitivities rose by 4% to 10%. Histology showed a trend for superiority over cytology only in characterizing nonadenocarcinoma tumor types. No differences in sensitivity were found between the centers involved. Retrospective design, different processing of cytologic specimens. At EUS-FNA in pancreatic masses, combined histologic-cytologic analysis achieved a sensitivity of more than 80%, despite a low number of needle passes and may thus save time. Histology alone did not reach higher sensitivity than cytology. In particular situations, eg, rare tumors, histology may still be required.
It has been established that comparative genomic hybridization (CGH) on Papanicolaou-stained cerv... more It has been established that comparative genomic hybridization (CGH) on Papanicolaou-stained cervical smears can be used to identify chromosomal imbalances. In this study, the authors identified normal and dysplastic squamous epithelial cells cytologically, eliminated surrounding bacteria or leukocytes by a ultraviolet laser microbeam under microscopic control, and scraped out the cell groups of interest by a microdissection system. In 3 cases of squamous intraepithelial lesions (SIL), a total of 9 samples of dysplastic (n = 6) and nontumorous cells (n = 3) were investigated, each of them consisting of 3-40 cells. The DNA was amplified by degenerate oligonucleotide primed PCR (DOP-PCR) and used for CGH. Analyses of all nontumorous cell groups resulted in fluorescence ratio profiles that showed no deviation from the normal range, confirming that no methodologic artefacts have been produced. The CGH profiles from dysplastic cells, however, showed various chromosomal imbalances affecting six to nine different chromosomes. The most frequent gains in DNA were observed on chromosomes 1p, 2q, 4, and 5, whereas losses were found on chromosomes 6q and 13q. The results of this study demonstrate the feasibility and reliability of CGH on microdissected cell samples of routinely processed cervical smears. To the authors' knowledge, this is the first study reporting the use of CGH on cervical routine smears. This approach offers the opportunity to investigate sequence copy number changes in small, morphologically well-defined groups of dysplastic cells. It may, therefore, serve as a cytogenetic screening test for identifying chromosomal aberrations in precancerous lesions that are associated with a high risk for progression to invasive cancer.
To assess continuous recording of the microscope status for quality assurance. Using a special mi... more To assess continuous recording of the microscope status for quality assurance. Using a special microscope with coordinate registration and objective decoding, 8,653 cervical smears were read by five experienced cytotechnologists. Cytotechnologists could check their screening pattern on a TV monitor. Stage position and magnification were registered every 20 msec. The average screening time per case was 3.5 minutes followed by an approximately two-minute intercase interval. Daily workload profiles were generated to check compliance with workload regulations. Average screening time over a day and over a week was rather constant. Our experience demonstrates that continuous coordinate registration can be easily implemented as a method of quality assurance in routine cytology.
Andrea Abati Fadi W. Abdul-Karim Pedro P. de Agustín Måns Åkerman M. Loredana Gadda Alasio Patric... more Andrea Abati Fadi W. Abdul-Karim Pedro P. de Agustín Måns Åkerman M. Loredana Gadda Alasio Patricia Alonso de Ruiz Carmen Alvarez-Santin Ana-Cristina Anton Karen M. Atkison Silvana Audy-Jurkovic Manon Auger R. Marshall Austin Jan P. A. Baak Ulrik Baandrup Gunter F. Bahr Peter H. Bartels Sandra H. Bigner John W. Bishop Alfred Böcking Miklós Bodó Vladimir N. Bogatyrev Thomas A. Bonfiglio Mathilde E. Boon Gerhard Breitenecker Ursula Buerzele-Fricke Jorge Campos R. de C. Carol A. Carriere Patricia Chapman William N. Christopherson Edith Claros Mercado Luiz Martins Collaco Brian T. Collins William N. Crabtree Peter Dalquen Dilip K. Das Diane D. Davey Irma DeLeón-Rodriguez Pranab Dey Manfred Droese Antonio Ducrot-Schinini Jaroslava Duskova S. Hamilton Dutoit Lars Egevad Hormoz Ehya Yener S. Erozan Dorothea Ferlin Clarice Amaral Ferreira Norman W. Fitzgerald Brendan T. Fitzpatrick Alessandra M. Forni Hugo Galera-Davidson Paolo Gattuso Kim R. Geisinger Claude Gompel Ricardo González-Cámpora Zenon Gonzalez-Romero Roberta M. Goodell Philippe de Graeve Shirley E. Greening Heinz K. Grohs Prabodh K. Gupta Raj K. Gupta Steven I. Hajdu Xhevdet Harasani Ernst Held Manuel Hilgarth Anders Hjerpe J. Heinrich Holzner O. A. N. Husain Martha L. Hutchinson Hideo Ikeda Lucrecia Illescas Stanley L. Inhorn Gita Jayaram Matías Jiménez-Ayala William W. Johnston David B. Kaminsky Kusum Kapila Harubumi Kato Ruth L. Katz William H. Kern Tadao K. Kobayashi Reviewers and Consultants
EUS-guided FNA (EUS-FNA) is an established tissue-acquisition technique, with most studies concen... more EUS-guided FNA (EUS-FNA) is an established tissue-acquisition technique, with most studies concentrating on cytologic analyses of specimens, with only few data existing on histologic assessment. To assess the sensitivity of a combined analysis of histologic followed by cytologic tissue diagnosis. A retrospective 3-center study. In consecutive patients undergoing FNA of solid pancreatic masses, core specimens were harvested for histology; residual tissue was examined cytologically. Only unequivocally positive results were regarded as malignant. Criterion standards were positive results from EUS-FNA or other histologic findings, or, if negative, clinical follow-up data (minimum 12 months). Among 192 patients (110 men; mean age 63 years) with mostly pancreatic-head masses (72.4%), overall, adequate tissue was obtained in 98.9% of all cases, with a mean of 1.88 needle passes and an overall sensitivity of 82.9% (95% CI, 76.0%-88.5%). Histology and subsequent cytology provided adequate tissue and sensitivities of 86.5% and 60%, and 92.7% and 68.1%, respectively. Excluding cases with inadequate specimens, sensitivities rose by 4% to 10%. Histology showed a trend for superiority over cytology only in characterizing nonadenocarcinoma tumor types. No differences in sensitivity were found between the centers involved. Retrospective design, different processing of cytologic specimens. At EUS-FNA in pancreatic masses, combined histologic-cytologic analysis achieved a sensitivity of more than 80%, despite a low number of needle passes and may thus save time. Histology alone did not reach higher sensitivity than cytology. In particular situations, eg, rare tumors, histology may still be required.
It has been established that comparative genomic hybridization (CGH) on Papanicolaou-stained cerv... more It has been established that comparative genomic hybridization (CGH) on Papanicolaou-stained cervical smears can be used to identify chromosomal imbalances. In this study, the authors identified normal and dysplastic squamous epithelial cells cytologically, eliminated surrounding bacteria or leukocytes by a ultraviolet laser microbeam under microscopic control, and scraped out the cell groups of interest by a microdissection system. In 3 cases of squamous intraepithelial lesions (SIL), a total of 9 samples of dysplastic (n = 6) and nontumorous cells (n = 3) were investigated, each of them consisting of 3-40 cells. The DNA was amplified by degenerate oligonucleotide primed PCR (DOP-PCR) and used for CGH. Analyses of all nontumorous cell groups resulted in fluorescence ratio profiles that showed no deviation from the normal range, confirming that no methodologic artefacts have been produced. The CGH profiles from dysplastic cells, however, showed various chromosomal imbalances affecting six to nine different chromosomes. The most frequent gains in DNA were observed on chromosomes 1p, 2q, 4, and 5, whereas losses were found on chromosomes 6q and 13q. The results of this study demonstrate the feasibility and reliability of CGH on microdissected cell samples of routinely processed cervical smears. To the authors' knowledge, this is the first study reporting the use of CGH on cervical routine smears. This approach offers the opportunity to investigate sequence copy number changes in small, morphologically well-defined groups of dysplastic cells. It may, therefore, serve as a cytogenetic screening test for identifying chromosomal aberrations in precancerous lesions that are associated with a high risk for progression to invasive cancer.
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