Behçet's disease (BD) is a chronic, systemic disease, characterized by oral and genital lesio... more Behçet's disease (BD) is a chronic, systemic disease, characterized by oral and genital lesions, and ocular inflammation. There is evidence indicating altered levels of proinflammatory cytokines, such as interleukin (IL)-6 and tumor necrosis factor alpha (TNF-α) in patients with BD. This study involved 150 patients with BD and 140 healthy controls, and investigated the role of proinflammatory cytokine gene polymorphisms in the disease. The frequency of the TNF-α (-238) G/G genotype was significantly higher in the patient group, compared to the controls (p < 0.001), whilst the G/A genotype was significantly lower in the patients with BD (p < 0.001). Patients with BD showed a significant increase in the TNF-α (- 308, - 238) GG haplotype (p < 0.001), whilst there was a significant decrease in the GA haplotype (p < 0.001). The heterozygous, IL-6 (- 174) C/G genotype (p = 0.005), and the IL-6 (- 174, nt565) haplotype CG (p < 0.001), were significantly decreased in the ...
Patients with systemic lupus erythematosus (SLE) are susceptible to infections, notably salmonell... more Patients with systemic lupus erythematosus (SLE) are susceptible to infections, notably salmonellosis. We report 37 cases of salmonella infection in 24 patients with SLE. These cases were detected in a group of 770 patients with SLE. All the patients were women, with a mean age of 25.6 years. At the onset of salmonella infection, 81 % were taking prednisolone and 27 % were taking cytotoxic drugs. Renal involvement was present in 75% of patients, which was approximately the same as in other SLE patients. Bacteremia, arthritis, osteomyelitis and rare manifestations of salmonellosis, including pulmonary and urinary tract involvement, were encountered. Diagnosis was based on isolation of the microorganism, mostly from blood cultures. Salmonella species other than typhi and paratyphi were often responsible. Widal agglutination test was positive in less than half the cases, and leukopenia was not seen frequently. Recurrence of infection in 29% of the patients and the high mortality rate (...
This preliminary study was conducted to discriminate the prevalence of Acanthamoeba antibodies in... more This preliminary study was conducted to discriminate the prevalence of Acanthamoeba antibodies in rheumatoid arthritis (RA) patients and healthy controls to analyze the correlation between these two groups. From October 2006 to August 2007 a total of 121 serum samples from RA patients attending the Rheumatolgy Department at Shariati Hospital in Tehran were obtained and stored at -20°C until using by indirect fluorescent-antibody test (IFAT). RA was diagnosed according to the American Collage of Rheumatology classification criteria. The organism used in this study was isolated from various water resources in Tehran, Iran cultured axenically and then went on a PCR assay based on 18S rRNA to identify the genus Acanthomoeba. Indirect immunofluorescence antibody (IFA) staining of serum samples was carried out to detect anti Acanthomoeba antibodies. In culture, out of 22 samples, 13(59%) were grown in xenic but only two in axenic medium. PCR amplified a 904bp fragment, specific for Acanth...
Background: Zinc was suggested as an antioxidant and immunomodulator trace element in parallel wi... more Background: Zinc was suggested as an antioxidant and immunomodulator trace element in parallel with other trace elements and antioxidant enzymes. Recently selenium deficiency was indicated in Behcet disease. In this study, we evaluated the correlation of serum zinc ...
Background: Selenium is known as an antioxidant and immunomodulator trace element. Recently, the ... more Background: Selenium is known as an antioxidant and immunomodulator trace element. Recently, the role of selenium deficiency in Behcet disease is discussed. This study performed to evaluate the correlation between serum selenium level and Behcet disease. Materials ...
The overlap syndrome is a vague entity in the, group of connective tissue diseases. It is unclear... more The overlap syndrome is a vague entity in the, group of connective tissue diseases. It is unclear whether it is fortuitous association of two or more connective tissue diseases (C'l'D). or a distinct disease. The early description of this syndrome was "a collection .symptoms of CTD and the existence of anti - nRN'P antibody". Many studies however, show that the anti • nRNP antibody is not specific for diagnosis. The aim of this study was to demonstrate the clinical and laboratory features of the overlap syndrome in 70 patients with SLE and 49 patients with PM1DM. The main features were then compared with those of the non overlapped disease (SLE or PMIDM) to search for any differences. Sharp, who first described this syndrome believed that die disease was a benign one. We compared the type of the treatment and the mortality rate. Our results seem to show that the overlap syndrome is not a fortuitous association of two or more diseases. 'There are differences separating an overlap svndrome from any of the original CTD. In contrast to the original belief of Sharp, the overlap syndrome is not a benign entity.
Acta Obstetricia et Gynecologica Scandinavica, 2005
... Acta Eur Fertil. 1984 Jan-Feb;15(1):67-70. 12. Akdag Kose A, Azizleri G, Sarica R, et al. The... more ... Acta Eur Fertil. 1984 Jan-Feb;15(1):67-70. 12. Akdag Kose A, Azizleri G, Sarica R, et al. The course of the Behcet&#x27;s disease during pregnancy. ... Nadji A, Shahram F, Davatchi F, et al. Behcet&#x27;s disease and Pregnancy. In: Hamza M, editor. Behcet&#x27;s disease. ...
Abstract Behçet's disease (BD) is a systemic and inflammatory disease, characterized mainly b... more Abstract Behçet's disease (BD) is a systemic and inflammatory disease, characterized mainly by recurrent oral and genital ulcers, eye involvement, and skin lesions. Although the exact etiopathogenesis of BD remains unrevealed, a bulk of studies have implicated the genetic contributing factors as critical players in disease predisposition. In countries along the Silk Road, human leukocyte antigen (HLA)-B51 has been reported as the strongest genetically associated factor for BD. Genome-wide association studies, local genetic polymorphism studies, and meta-analysis of combined data from Turkish, Iranian, and Japanese populations have also identified new genetic associations with BD. Among these, other HLA alleles such as HLA-B*15, HLA-B*27, HLA-B*57, and HLA-A*26 have been found as independent risk factors for BD, whereas HLA-B*49 and HLA-A*03 are independent protective alleles for BD. Moreover, other genes have also reached the genome-wide significance level of association with BD susceptibility, including IL10, IL23R-IL12RB2, IL12A, CCR1-CCR3, STAT4, TNFAIP3, ERAP1, KLRC4, and FUT2. Also, several rare nonsynonymous variants in TLR4, IL23R, NOD2, and MEFV genes have been reported to be involved in BD pathogenesis. According to genetic determinants in the loci outside the MHC region that are contributed to the host defense, immunity, and inflammation pathways, it is suggested that immune responses to the pathogen as an important environmental factor and mucosal immunity contribute to BD susceptibility.
Objective: Overwhelming inflammatory chemokines and cytokines characterize the immunological prof... more Objective: Overwhelming inflammatory chemokines and cytokines characterize the immunological profile and inflammatory settings of Behcet disease (BD). The connection between autophagy-related genes (ATGs) and various perspectives of innate and adaptive immunobiology such as antigen presentation, immune tolerance, lymphocyte development and differentiation, cytokine signaling, and inflammation have been implicated. The aim of this study was to evaluate the mRNA expression profile of ATGs in macrophages of patients with BD. Materials and Methods: Whole blood samples were obtained from 10 BD patients and 10 healthy controls. Monocytes were isolated from the blood samples and then differentiated to macrophages using macrophage colony-stimulating factor (M-CSF). After total RNA extraction and cDNA synthesis, quantitative analysis of ATGs including ATG5, ATG7, ATG12, LC3b, mTOR, RAPTOR, and RICTOR was conducted by SYBR Green master mix and real-time polymerase chain reaction (PCR). Result...
Introduction Behcet disease (BD) is a multisystemic and chronic inflammatory disorder, mainly cha... more Introduction Behcet disease (BD) is a multisystemic and chronic inflammatory disorder, mainly characterized by recurrent oral and genital ulcers, ocular involvement, and skin lesions, with periods of remission and relapse (1,2). Epidemiological studies have documented that BD is comparatively frequent in countries located around Mediterranean Sea, the Middle East, China, and Japan, along the ancient Silk Route (3). The exact etiology of BD has not fully explained yet, but the disease occurrence is highly associated with a genetic factor, namely human leukocyte antigen (HLA)-B51. Furthermore, genetic studies have established a strong association of HLA-B with cytokine production including high production of tumor necrosis factor (TNF) and decreased production of interleukin (IL)-10. Gene variations have also been observed in inflammatory cytokines, chemokines, and adhesion molecules, leading to vascular inflammation and consequently vascular damage (4,5). BD shares characteristics of...
Endoplasmic reticulum (ER) stress triggers the unfolded protein response (UPR), which has been co... more Endoplasmic reticulum (ER) stress triggers the unfolded protein response (UPR), which has been correlated with enhancedproduction of inflammatory cytokines. Given the important pathogenic roles of macrophages and inflammatory responses in theetiopathogenesis of Behcet’s disease (BD), this study aimed to assess the mRNA expression pattern of genes involved in theUPR pathway in macrophages from smoker and non-smoker BD patients. This case-control study was conducted between 2015and 2016 in Shariati Hospital, Tehran, Iran. Monocytes were enriched from obtained whole blood samples of 10 smokers and 10non-smoker BD patients as well as 10 healthy individuals. Using macrophage-colony stimulating factor (M-CSF), separatedmonocytes were differentiated into macrophages. After total RNA purification and cDNA synthesis, quantification analysis ofUPR genes, including activating transcription factor (ATF) 4, ATF6, X-box binding protein 1 (XBP1), binding immunoglobulinprotein (BIP), C/EBP homologo...
Behçet's disease (BD) is a chronic, systemic disease, characterized by oral and genital lesio... more Behçet's disease (BD) is a chronic, systemic disease, characterized by oral and genital lesions, and ocular inflammation. There is evidence indicating altered levels of proinflammatory cytokines, such as interleukin (IL)-6 and tumor necrosis factor alpha (TNF-α) in patients with BD. This study involved 150 patients with BD and 140 healthy controls, and investigated the role of proinflammatory cytokine gene polymorphisms in the disease. The frequency of the TNF-α (-238) G/G genotype was significantly higher in the patient group, compared to the controls (p < 0.001), whilst the G/A genotype was significantly lower in the patients with BD (p < 0.001). Patients with BD showed a significant increase in the TNF-α (- 308, - 238) GG haplotype (p < 0.001), whilst there was a significant decrease in the GA haplotype (p < 0.001). The heterozygous, IL-6 (- 174) C/G genotype (p = 0.005), and the IL-6 (- 174, nt565) haplotype CG (p < 0.001), were significantly decreased in the ...
Patients with systemic lupus erythematosus (SLE) are susceptible to infections, notably salmonell... more Patients with systemic lupus erythematosus (SLE) are susceptible to infections, notably salmonellosis. We report 37 cases of salmonella infection in 24 patients with SLE. These cases were detected in a group of 770 patients with SLE. All the patients were women, with a mean age of 25.6 years. At the onset of salmonella infection, 81 % were taking prednisolone and 27 % were taking cytotoxic drugs. Renal involvement was present in 75% of patients, which was approximately the same as in other SLE patients. Bacteremia, arthritis, osteomyelitis and rare manifestations of salmonellosis, including pulmonary and urinary tract involvement, were encountered. Diagnosis was based on isolation of the microorganism, mostly from blood cultures. Salmonella species other than typhi and paratyphi were often responsible. Widal agglutination test was positive in less than half the cases, and leukopenia was not seen frequently. Recurrence of infection in 29% of the patients and the high mortality rate (...
This preliminary study was conducted to discriminate the prevalence of Acanthamoeba antibodies in... more This preliminary study was conducted to discriminate the prevalence of Acanthamoeba antibodies in rheumatoid arthritis (RA) patients and healthy controls to analyze the correlation between these two groups. From October 2006 to August 2007 a total of 121 serum samples from RA patients attending the Rheumatolgy Department at Shariati Hospital in Tehran were obtained and stored at -20°C until using by indirect fluorescent-antibody test (IFAT). RA was diagnosed according to the American Collage of Rheumatology classification criteria. The organism used in this study was isolated from various water resources in Tehran, Iran cultured axenically and then went on a PCR assay based on 18S rRNA to identify the genus Acanthomoeba. Indirect immunofluorescence antibody (IFA) staining of serum samples was carried out to detect anti Acanthomoeba antibodies. In culture, out of 22 samples, 13(59%) were grown in xenic but only two in axenic medium. PCR amplified a 904bp fragment, specific for Acanth...
Background: Zinc was suggested as an antioxidant and immunomodulator trace element in parallel wi... more Background: Zinc was suggested as an antioxidant and immunomodulator trace element in parallel with other trace elements and antioxidant enzymes. Recently selenium deficiency was indicated in Behcet disease. In this study, we evaluated the correlation of serum zinc ...
Background: Selenium is known as an antioxidant and immunomodulator trace element. Recently, the ... more Background: Selenium is known as an antioxidant and immunomodulator trace element. Recently, the role of selenium deficiency in Behcet disease is discussed. This study performed to evaluate the correlation between serum selenium level and Behcet disease. Materials ...
The overlap syndrome is a vague entity in the, group of connective tissue diseases. It is unclear... more The overlap syndrome is a vague entity in the, group of connective tissue diseases. It is unclear whether it is fortuitous association of two or more connective tissue diseases (C'l'D). or a distinct disease. The early description of this syndrome was "a collection .symptoms of CTD and the existence of anti - nRN'P antibody". Many studies however, show that the anti • nRNP antibody is not specific for diagnosis. The aim of this study was to demonstrate the clinical and laboratory features of the overlap syndrome in 70 patients with SLE and 49 patients with PM1DM. The main features were then compared with those of the non overlapped disease (SLE or PMIDM) to search for any differences. Sharp, who first described this syndrome believed that die disease was a benign one. We compared the type of the treatment and the mortality rate. Our results seem to show that the overlap syndrome is not a fortuitous association of two or more diseases. 'There are differences separating an overlap svndrome from any of the original CTD. In contrast to the original belief of Sharp, the overlap syndrome is not a benign entity.
Acta Obstetricia et Gynecologica Scandinavica, 2005
... Acta Eur Fertil. 1984 Jan-Feb;15(1):67-70. 12. Akdag Kose A, Azizleri G, Sarica R, et al. The... more ... Acta Eur Fertil. 1984 Jan-Feb;15(1):67-70. 12. Akdag Kose A, Azizleri G, Sarica R, et al. The course of the Behcet&#x27;s disease during pregnancy. ... Nadji A, Shahram F, Davatchi F, et al. Behcet&#x27;s disease and Pregnancy. In: Hamza M, editor. Behcet&#x27;s disease. ...
Abstract Behçet's disease (BD) is a systemic and inflammatory disease, characterized mainly b... more Abstract Behçet's disease (BD) is a systemic and inflammatory disease, characterized mainly by recurrent oral and genital ulcers, eye involvement, and skin lesions. Although the exact etiopathogenesis of BD remains unrevealed, a bulk of studies have implicated the genetic contributing factors as critical players in disease predisposition. In countries along the Silk Road, human leukocyte antigen (HLA)-B51 has been reported as the strongest genetically associated factor for BD. Genome-wide association studies, local genetic polymorphism studies, and meta-analysis of combined data from Turkish, Iranian, and Japanese populations have also identified new genetic associations with BD. Among these, other HLA alleles such as HLA-B*15, HLA-B*27, HLA-B*57, and HLA-A*26 have been found as independent risk factors for BD, whereas HLA-B*49 and HLA-A*03 are independent protective alleles for BD. Moreover, other genes have also reached the genome-wide significance level of association with BD susceptibility, including IL10, IL23R-IL12RB2, IL12A, CCR1-CCR3, STAT4, TNFAIP3, ERAP1, KLRC4, and FUT2. Also, several rare nonsynonymous variants in TLR4, IL23R, NOD2, and MEFV genes have been reported to be involved in BD pathogenesis. According to genetic determinants in the loci outside the MHC region that are contributed to the host defense, immunity, and inflammation pathways, it is suggested that immune responses to the pathogen as an important environmental factor and mucosal immunity contribute to BD susceptibility.
Objective: Overwhelming inflammatory chemokines and cytokines characterize the immunological prof... more Objective: Overwhelming inflammatory chemokines and cytokines characterize the immunological profile and inflammatory settings of Behcet disease (BD). The connection between autophagy-related genes (ATGs) and various perspectives of innate and adaptive immunobiology such as antigen presentation, immune tolerance, lymphocyte development and differentiation, cytokine signaling, and inflammation have been implicated. The aim of this study was to evaluate the mRNA expression profile of ATGs in macrophages of patients with BD. Materials and Methods: Whole blood samples were obtained from 10 BD patients and 10 healthy controls. Monocytes were isolated from the blood samples and then differentiated to macrophages using macrophage colony-stimulating factor (M-CSF). After total RNA extraction and cDNA synthesis, quantitative analysis of ATGs including ATG5, ATG7, ATG12, LC3b, mTOR, RAPTOR, and RICTOR was conducted by SYBR Green master mix and real-time polymerase chain reaction (PCR). Result...
Introduction Behcet disease (BD) is a multisystemic and chronic inflammatory disorder, mainly cha... more Introduction Behcet disease (BD) is a multisystemic and chronic inflammatory disorder, mainly characterized by recurrent oral and genital ulcers, ocular involvement, and skin lesions, with periods of remission and relapse (1,2). Epidemiological studies have documented that BD is comparatively frequent in countries located around Mediterranean Sea, the Middle East, China, and Japan, along the ancient Silk Route (3). The exact etiology of BD has not fully explained yet, but the disease occurrence is highly associated with a genetic factor, namely human leukocyte antigen (HLA)-B51. Furthermore, genetic studies have established a strong association of HLA-B with cytokine production including high production of tumor necrosis factor (TNF) and decreased production of interleukin (IL)-10. Gene variations have also been observed in inflammatory cytokines, chemokines, and adhesion molecules, leading to vascular inflammation and consequently vascular damage (4,5). BD shares characteristics of...
Endoplasmic reticulum (ER) stress triggers the unfolded protein response (UPR), which has been co... more Endoplasmic reticulum (ER) stress triggers the unfolded protein response (UPR), which has been correlated with enhancedproduction of inflammatory cytokines. Given the important pathogenic roles of macrophages and inflammatory responses in theetiopathogenesis of Behcet’s disease (BD), this study aimed to assess the mRNA expression pattern of genes involved in theUPR pathway in macrophages from smoker and non-smoker BD patients. This case-control study was conducted between 2015and 2016 in Shariati Hospital, Tehran, Iran. Monocytes were enriched from obtained whole blood samples of 10 smokers and 10non-smoker BD patients as well as 10 healthy individuals. Using macrophage-colony stimulating factor (M-CSF), separatedmonocytes were differentiated into macrophages. After total RNA purification and cDNA synthesis, quantification analysis ofUPR genes, including activating transcription factor (ATF) 4, ATF6, X-box binding protein 1 (XBP1), binding immunoglobulinprotein (BIP), C/EBP homologo...
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