The diurnal variations of the endocannabinoid arachidonoylethanolamine (anandamide, ANA) as well ... more The diurnal variations of the endocannabinoid arachidonoylethanolamine (anandamide, ANA) as well as palmitoylethanolamide (PEA) and oleoylethanolamide (OEA) were detected and quantified in cerebrospinal fluid (CSF), pons, hippocampus, and hypothalamus in the rat over 24 h using HPLC/MS.In CSF, the 3 compounds presented an increase in their concentration during the lights-on period and a remarkable decrease in their values during the lights-off period. In the pons, ANA, PEA and OEA showed the maximum values during the dark phase. On the other hand, we found that in the hippocampus, ANA increased its concentration during the lights-off period and PEA showed the highest peak at the beginning of the same period. OEA concentration showed no diurnal variations in the hippocampus. Finally, in the hypothalamus, ANA rose during the lights-on period whereas PEA and OEA presented the highest concentration at the end of the lights-off period.We postulate that all compounds are likely to be accumulated in parenchyma during the lights-off period (when animal is awake) and then, released into the CSF in order to reach target regions in turn to modulate diverse behaviors, such as feeding and sleep.
The effectiveness of Bio-Glas 200 and 500 for separation and molecular weight estimation of polyp... more The effectiveness of Bio-Glas 200 and 500 for separation and molecular weight estimation of polypeptide chains in 6 m guanidine hydrochloride was investigated. The distribution coefficients of 10 proteins after reduction and alkylation were determined. We found that the columns gave good calibration curves when pure proteins were passed individually. But the resolving power of Bio-Glas 200 and 500 in this solvent was very poor, apparently because of their high matrix volume and low inner volume.
The diurnal variations of the endocannabinoid arachidonoylethanolamine (anandamide, ANA) as well ... more The diurnal variations of the endocannabinoid arachidonoylethanolamine (anandamide, ANA) as well as palmitoylethanolamide (PEA) and oleoylethanolamide (OEA) were detected and quantified in cerebrospinal fluid (CSF), pons, hippocampus, and hypothalamus in the rat over 24 h using HPLC/MS.In CSF, the 3 compounds presented an increase in their concentration during the lights-on period and a remarkable decrease in their values during the lights-off period. In the pons, ANA, PEA and OEA showed the maximum values during the dark phase. On the other hand, we found that in the hippocampus, ANA increased its concentration during the lights-off period and PEA showed the highest peak at the beginning of the same period. OEA concentration showed no diurnal variations in the hippocampus. Finally, in the hypothalamus, ANA rose during the lights-on period whereas PEA and OEA presented the highest concentration at the end of the lights-off period.We postulate that all compounds are likely to be accumulated in parenchyma during the lights-off period (when animal is awake) and then, released into the CSF in order to reach target regions in turn to modulate diverse behaviors, such as feeding and sleep.
The effectiveness of Bio-Glas 200 and 500 for separation and molecular weight estimation of polyp... more The effectiveness of Bio-Glas 200 and 500 for separation and molecular weight estimation of polypeptide chains in 6 m guanidine hydrochloride was investigated. The distribution coefficients of 10 proteins after reduction and alkylation were determined. We found that the columns gave good calibration curves when pure proteins were passed individually. But the resolving power of Bio-Glas 200 and 500 in this solvent was very poor, apparently because of their high matrix volume and low inner volume.
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Papers by Ana Rose