International journal of systematic and evolutionary microbiology, Jan 21, 2018
Pectobacterium isolates SS95, SS54 and SS56 were collected from a potato field in the Chiniot dis... more Pectobacterium isolates SS95, SS54 and SS56 were collected from a potato field in the Chiniot district in the plains of the Punjab province, Pakistan. Sequencing of the gapA barcode revealed that these strains belong to a novel phylogenetic group separated from P.ectobacterium wasabiae and Pectobacterium parmentieri species. Furthermore, multilocus sequence analyses of 13 housekeeping genes (fusA, rpoD, acnA, purA, gyrB, recA, mdh, mtlD, groEL, secY, glyA, gapA and rplB) clearly distinguished the type strain, SS95, from its closest relatives, i.e. P. parmentieri RNS 08-42-1A and P. wasabiae CFBP3304, as well as from all the other known Pectobacteriumspecies. In silico DNA-DNA hybridization (<44.1 %) and average nucleotide identity (<90.75 %) values of strain SS95 compared with other Pectobacterium type strains supported the delineation of a new species. Genomic and phenotypic comparisons permitted the identification of additional traits that distinguished the Pakistani isolate...
Environmental science and pollution research international, Jan 23, 2018
Biomass is a promising renewable energy source and its significance is escalating in the context ... more Biomass is a promising renewable energy source and its significance is escalating in the context of climate change and depletion of fossil foils. This study was conducted for two consecutive years 2016 and 2017, using five sorghum cultivars, i.e., JS-263, Jawar-2011, Hagari, JS-2002, and YS-2016, in order to determine the best cultivars in terms of dry matter yield, chemical composition, and biomethane yield grown under semi-arid conditions in Pakistan. The results revealed that sorghum cultivars responded differently in terms of growth, biomass yield, chemical composition, and methane yield. Cultivars Jawar-2011 produced maximum leaf area index, leaf area duration, crop growth rate, plant height, and leaves per plant, however, they were comparable with Sorghum-2016, whereas cultivar JS-2002 performed poorly among the tested cultivars. Similarly, cultivar Jawar-2011 produced maximum dry matter yield (16.37 t ha) similar to that of YS-2016, further cultivar JS-2002 performed poorly a...
Authors&#39; Contribution ARK, SAK and WA designed the study, executed experimental work and ... more Authors&#39; Contribution ARK, SAK and WA designed the study, executed experimental work and analyzed the data. NJ and STS supervised the work. TM helped in preparation of the manuscript.
Minerals are the crucial part of plant nutrition an d their presence in excess or deficiencies ma... more Minerals are the crucial part of plant nutrition an d their presence in excess or deficiencies may cause certain maladies in the plan ts either through disturbing metabolism or plant physiology abnormally by favoring the plant pathoge ns or discouraging the plant growth. Potassium provides, stress such as frost and diseas e tolerance ability to potato plants. Potassium content was tested from three different samples tak en at different times, first after 50 days of planting of the crop when there was no disease appe ared while second samples were taken from healthy and diseased plant individually, almost 30 days after the first appearance of the symptoms on late blight. Results of present study revealed t hat there was an overall decrease in the potassium concentration of leaves in all tested lines after d isease appearance. This decrease was ranged from 2.8 to 13.6 percent over the healthy plants of that same age group. Genotypes FD 8-1 , N-22 and SH 788 proved to be most susceptible genotype in term reduction in leaf potassium concentration by losing 13.6, 13.5 and 13.4 percent of potassium due to late blight infestation, while 2.8 percent decrease of potassium content was observed in line FD 32-2. Decrease in potassium content was also observed in the healthy plants of all potato l ines/cultivars which were not infected by the disease but this decrease might be due to aging fac tor and was quit insignificant over the plants on which disease was not appeared and unnoticeable. This decrease in potassium content of diseased plant in all lines/cultivars was statistically sign ificant. Although the potassium content was slight ly decreased in the plants which were not diseased tha n that of healthy plants which were tested at the time when disease was not appeared. Decrease in potassium content was more pronounced due to the effect of disease and there was no or negligibl e effect of aging was observed.
The ability of salicylic acid and jasmonic acid to suppress post-harvest infection with green mou... more The ability of salicylic acid and jasmonic acid to suppress post-harvest infection with green mould Penicillium digitatum and blue mould P. italicum on three citrus species Citrus reticulata ‘Kinnow’, C. limon ‘Meyer Lemon’, and C. limetta ‘Mosambi’ was evaluated in a dose-response study. Salicylic acid (SA) and jasmonic acid (JA) were applied to the fruits as a post-harvest dip treatment followed by wound inoculation with the pathogens. Both resistance inducers caused a significantly lower disease severity compared with the infected but non-treated control, whereas disease incidence was not significantly lower than in the control. The efficacy of both SA and JA in reducing disease severity was concentration-dependent; the use of higher concentrations resulted in a greater degree of suppression. All the Citrus species tested in this study showed different responses in terms of disease development. C. limon ‘Meyer Lemon’ showed the highest disease development, and C. limetta ‘Mosambi...
ABSTRACT All the four fungi isolated from Cuscuta stem produced black necrotic lesions on Cuscuta... more ABSTRACT All the four fungi isolated from Cuscuta stem produced black necrotic lesions on Cuscuta when inoculated artificially. Colletotrichum gloeosporioides followed by Fusarium pallidoroseum and Alternarai alternata were most destructive when inoculated artificially separately. Severe attack was observed where Alternarai alternata, Curvularia lunata, Colletotrichum gloeosporioides and Fusarium pallidoroseum were inoculated artificially in combinations.
ABSTRACT In vitro studies on physiology of four fungi namely Alternaria alternata, Colltotrichum ... more ABSTRACT In vitro studies on physiology of four fungi namely Alternaria alternata, Colltotrichum gloeosporioides, Curvularia lunata and Fusarium pallidoroseum isolated from Cuscuta stem cuttings were conducted. All the fungi gave the best mycelial growth on universal medium. Out of these fungi A. alternata and F. pallidoroseum gave the best growth at 25°C and C. gloeosporioides gave the best mycelial growth at 20?C while C. gloeosporioides required pH 6 for better mycelial growth. 24 hours continuous light favoured the growth of A. alternata and C. gloeosporioides while C. lunata and F. pallidoroseum gave better results under 12 hours light and 12 hours darkness.
... AND WAQAS WAKIL 2 ... The disease can be managed by the removal and destruction of dead plant... more ... AND WAQAS WAKIL 2 ... The disease can be managed by the removal and destruction of dead plant debris, crop rotation, deep sowing of seed (Sattar, 1933), inter-cropping of chickpea with cereals (Luthra &amp;amp;amp;amp;amp; Bedi, 1935), by fungicidal seed treatment (Tripathi et al., 1987) and ...
International journal of systematic and evolutionary microbiology, Jan 21, 2018
Pectobacterium isolates SS95, SS54 and SS56 were collected from a potato field in the Chiniot dis... more Pectobacterium isolates SS95, SS54 and SS56 were collected from a potato field in the Chiniot district in the plains of the Punjab province, Pakistan. Sequencing of the gapA barcode revealed that these strains belong to a novel phylogenetic group separated from P.ectobacterium wasabiae and Pectobacterium parmentieri species. Furthermore, multilocus sequence analyses of 13 housekeeping genes (fusA, rpoD, acnA, purA, gyrB, recA, mdh, mtlD, groEL, secY, glyA, gapA and rplB) clearly distinguished the type strain, SS95, from its closest relatives, i.e. P. parmentieri RNS 08-42-1A and P. wasabiae CFBP3304, as well as from all the other known Pectobacteriumspecies. In silico DNA-DNA hybridization (<44.1 %) and average nucleotide identity (<90.75 %) values of strain SS95 compared with other Pectobacterium type strains supported the delineation of a new species. Genomic and phenotypic comparisons permitted the identification of additional traits that distinguished the Pakistani isolate...
Environmental science and pollution research international, Jan 23, 2018
Biomass is a promising renewable energy source and its significance is escalating in the context ... more Biomass is a promising renewable energy source and its significance is escalating in the context of climate change and depletion of fossil foils. This study was conducted for two consecutive years 2016 and 2017, using five sorghum cultivars, i.e., JS-263, Jawar-2011, Hagari, JS-2002, and YS-2016, in order to determine the best cultivars in terms of dry matter yield, chemical composition, and biomethane yield grown under semi-arid conditions in Pakistan. The results revealed that sorghum cultivars responded differently in terms of growth, biomass yield, chemical composition, and methane yield. Cultivars Jawar-2011 produced maximum leaf area index, leaf area duration, crop growth rate, plant height, and leaves per plant, however, they were comparable with Sorghum-2016, whereas cultivar JS-2002 performed poorly among the tested cultivars. Similarly, cultivar Jawar-2011 produced maximum dry matter yield (16.37 t ha) similar to that of YS-2016, further cultivar JS-2002 performed poorly a...
Authors&#39; Contribution ARK, SAK and WA designed the study, executed experimental work and ... more Authors&#39; Contribution ARK, SAK and WA designed the study, executed experimental work and analyzed the data. NJ and STS supervised the work. TM helped in preparation of the manuscript.
Minerals are the crucial part of plant nutrition an d their presence in excess or deficiencies ma... more Minerals are the crucial part of plant nutrition an d their presence in excess or deficiencies may cause certain maladies in the plan ts either through disturbing metabolism or plant physiology abnormally by favoring the plant pathoge ns or discouraging the plant growth. Potassium provides, stress such as frost and diseas e tolerance ability to potato plants. Potassium content was tested from three different samples tak en at different times, first after 50 days of planting of the crop when there was no disease appe ared while second samples were taken from healthy and diseased plant individually, almost 30 days after the first appearance of the symptoms on late blight. Results of present study revealed t hat there was an overall decrease in the potassium concentration of leaves in all tested lines after d isease appearance. This decrease was ranged from 2.8 to 13.6 percent over the healthy plants of that same age group. Genotypes FD 8-1 , N-22 and SH 788 proved to be most susceptible genotype in term reduction in leaf potassium concentration by losing 13.6, 13.5 and 13.4 percent of potassium due to late blight infestation, while 2.8 percent decrease of potassium content was observed in line FD 32-2. Decrease in potassium content was also observed in the healthy plants of all potato l ines/cultivars which were not infected by the disease but this decrease might be due to aging fac tor and was quit insignificant over the plants on which disease was not appeared and unnoticeable. This decrease in potassium content of diseased plant in all lines/cultivars was statistically sign ificant. Although the potassium content was slight ly decreased in the plants which were not diseased tha n that of healthy plants which were tested at the time when disease was not appeared. Decrease in potassium content was more pronounced due to the effect of disease and there was no or negligibl e effect of aging was observed.
The ability of salicylic acid and jasmonic acid to suppress post-harvest infection with green mou... more The ability of salicylic acid and jasmonic acid to suppress post-harvest infection with green mould Penicillium digitatum and blue mould P. italicum on three citrus species Citrus reticulata ‘Kinnow’, C. limon ‘Meyer Lemon’, and C. limetta ‘Mosambi’ was evaluated in a dose-response study. Salicylic acid (SA) and jasmonic acid (JA) were applied to the fruits as a post-harvest dip treatment followed by wound inoculation with the pathogens. Both resistance inducers caused a significantly lower disease severity compared with the infected but non-treated control, whereas disease incidence was not significantly lower than in the control. The efficacy of both SA and JA in reducing disease severity was concentration-dependent; the use of higher concentrations resulted in a greater degree of suppression. All the Citrus species tested in this study showed different responses in terms of disease development. C. limon ‘Meyer Lemon’ showed the highest disease development, and C. limetta ‘Mosambi...
ABSTRACT All the four fungi isolated from Cuscuta stem produced black necrotic lesions on Cuscuta... more ABSTRACT All the four fungi isolated from Cuscuta stem produced black necrotic lesions on Cuscuta when inoculated artificially. Colletotrichum gloeosporioides followed by Fusarium pallidoroseum and Alternarai alternata were most destructive when inoculated artificially separately. Severe attack was observed where Alternarai alternata, Curvularia lunata, Colletotrichum gloeosporioides and Fusarium pallidoroseum were inoculated artificially in combinations.
ABSTRACT In vitro studies on physiology of four fungi namely Alternaria alternata, Colltotrichum ... more ABSTRACT In vitro studies on physiology of four fungi namely Alternaria alternata, Colltotrichum gloeosporioides, Curvularia lunata and Fusarium pallidoroseum isolated from Cuscuta stem cuttings were conducted. All the fungi gave the best mycelial growth on universal medium. Out of these fungi A. alternata and F. pallidoroseum gave the best growth at 25°C and C. gloeosporioides gave the best mycelial growth at 20?C while C. gloeosporioides required pH 6 for better mycelial growth. 24 hours continuous light favoured the growth of A. alternata and C. gloeosporioides while C. lunata and F. pallidoroseum gave better results under 12 hours light and 12 hours darkness.
... AND WAQAS WAKIL 2 ... The disease can be managed by the removal and destruction of dead plant... more ... AND WAQAS WAKIL 2 ... The disease can be managed by the removal and destruction of dead plant debris, crop rotation, deep sowing of seed (Sattar, 1933), inter-cropping of chickpea with cereals (Luthra &amp;amp;amp;amp;amp; Bedi, 1935), by fungicidal seed treatment (Tripathi et al., 1987) and ...
Uploads
Papers by Shahbaz Sahi