The purpose of the present work has been to evaluate the biological activity of alkaloids and tan... more The purpose of the present work has been to evaluate the biological activity of alkaloids and tannins extracted from roots of Punica granatum L. on axenic cultures from Entamoeba histolytica and E. invadens, strains. Initially, the total aqueous extract and later some of its components obtained by thin layer chromatography were tested. These compounds were identified by chromatography as alkaloids or tannins. The density of amebic cultures was determined with a hemocytometer after 48 and 96 hours of incubation, which was calculated by the difference between number of trophozoites obtained at the times chosen and the number of amoebae inoculated. Two milliliters of aqueous extract had higher activity on cultures from E. histolytica than E. invadens strains, producing growth inhibitions of about 100 and 40 per cent respectively. Alkaloid concentrations of 1 mg/ml had no amebicide activity, however tannins at concentrations of 10 micrograms/ml for E. histolytica, and 100 micrograms/ml for E. invadens were sufficient to produce an growth inhibition about 100 per cent. Tannic acid was also tested on the cultures of E. histolytica observing an high inhibitory activity on the growth, this effect was produced at 0.01 mg/ml was similar to that observed with the tannins mixture.
Four dynamic conditions to evaluate the effects of stress on production of biomass, essential oil... more Four dynamic conditions to evaluate the effects of stress on production of biomass, essential oils, Thymol, carvacrol and proline accumulation in Lippia graveolens H.B.K. were inves- tigated under greenhouse conditions. A bifactorial design assessed four environmental conditions (factor B): commercial nutritive solu- tion (B0); Water (B1); moderate stress (B2), and intermediate (B3) stress nested under (Factor A) salinity (NaCl) and ion concentration (Cu 2+ ) and (Fe 2+ ) stresses. Average production of fresh weight and number of leaves were reduced under NaCl and both ion concentra- tion conditions. However, there were no significant differences on average for the four environments. The amounts of essential oils were significantly higher under the stressful environments with Fe
In order to preserve and use the genetic resources of the arid and semi-arid and arid lands of Me... more In order to preserve and use the genetic resources of the arid and semi-arid and arid lands of Mexico, 35 wild and 6 cultivated samples of Lippia sp. (oregano) from General Cepeda, Ramos Arizpe, Parras de la Fuente and Matamoros in Coahuila State were analyzed. The molecular description was made through Random Amplified Polymorphic DNA (RAPD) and Simple Sequences Repeated (SSR). In addition, the quantity and quality of essential oils was determined by means of the drag technique by steam and high resolution liquid chromatography. RAPD and SSR markers were able to detect a high level of polymorphism in oregano. However, it was not possible to differentiate the whole assessed collections as well as to gather them in regard to location. In contrast, it was determined that the genetic diversity was mild (47%) and higher among populations. Some samples showed high values of carvacrol while thymol was very heterogeneous among populations. It is concluded that there is enough variation in ...
Some biochemical basis for tolerance of bean cultivars to low nutrient conditions are discussed. ... more Some biochemical basis for tolerance of bean cultivars to low nutrient conditions are discussed. Two tolerant bean cultivars (Flor de Mayo & Laguna) under low nutrient stress accumulated more proline than sensitive varieties (Garbancillo & Pinamerpa). A 38 kDa protein reached higher concentrations in the tolerant cultivars. Laguna and Pinamerpa contained more chlorophyll than the other varieties. It is suggested that proline accumulation and the specific protein may be involved in the adaptation to marginal nutrient situations.
The paper describes diverse aspects of the floral biology including development of floral buds, a... more The paper describes diverse aspects of the floral biology including development of floral buds, anthesis, the sequential process of microsporogenesis. Different stages of fruit development involving color changes during the process of maturity are also discribed.
Para comprobar el efecto de dosis del producto comercial Algaenzims sobre plantulas de 30 dias de... more Para comprobar el efecto de dosis del producto comercial Algaenzims sobre plantulas de 30 dias de dos variedades de sorgo (Rio Bravo y P-83G66) se evaluO: longitud total, longitud de la raiz, longitud de tallo, n´umero de hojas por planta, grosor de tallo, largo y ancho de hoja ligulada, largo y ancho de hojas. Hubo diferencias altamente significativas (P<0.01) y significativas (P<0.05) para longitud de tallo, largo y ancho de hoja, n´umero de hojas por planta y ancho de hoja ligulada. Se analizO tambien, mediante espectrofotometria de emisiOn por plasma, el contenido de Mg, Na, K, Mo, Mn, Ni, Cu, Zn y Cd, tanto en tallo como en raiz, encontrandose diferencias altamente significativas en el contenido de estos minerales tanto entre variedades como entre tipos de tratamientos y variedades.
The purpose of the present work has been to evaluate the biological activity of alkaloids and tan... more The purpose of the present work has been to evaluate the biological activity of alkaloids and tannins extracted from roots of Punica granatum L. on axenic cultures from Entamoeba histolytica and E. invadens, strains. Initially, the total aqueous extract and later some of its components obtained by thin layer chromatography were tested. These compounds were identified by chromatography as alkaloids or tannins. The density of amebic cultures was determined with a hemocytometer after 48 and 96 hours of incubation, which was calculated by the difference between number of trophozoites obtained at the times chosen and the number of amoebae inoculated. Two milliliters of aqueous extract had higher activity on cultures from E. histolytica than E. invadens strains, producing growth inhibitions of about 100 and 40 per cent respectively. Alkaloid concentrations of 1 mg/ml had no amebicide activity, however tannins at concentrations of 10 micrograms/ml for E. histolytica, and 100 micrograms/ml for E. invadens were sufficient to produce an growth inhibition about 100 per cent. Tannic acid was also tested on the cultures of E. histolytica observing an high inhibitory activity on the growth, this effect was produced at 0.01 mg/ml was similar to that observed with the tannins mixture.
Four dynamic conditions to evaluate the effects of stress on production of biomass, essential oil... more Four dynamic conditions to evaluate the effects of stress on production of biomass, essential oils, Thymol, carvacrol and proline accumulation in Lippia graveolens H.B.K. were inves- tigated under greenhouse conditions. A bifactorial design assessed four environmental conditions (factor B): commercial nutritive solu- tion (B0); Water (B1); moderate stress (B2), and intermediate (B3) stress nested under (Factor A) salinity (NaCl) and ion concentration (Cu 2+ ) and (Fe 2+ ) stresses. Average production of fresh weight and number of leaves were reduced under NaCl and both ion concentra- tion conditions. However, there were no significant differences on average for the four environments. The amounts of essential oils were significantly higher under the stressful environments with Fe
In order to preserve and use the genetic resources of the arid and semi-arid and arid lands of Me... more In order to preserve and use the genetic resources of the arid and semi-arid and arid lands of Mexico, 35 wild and 6 cultivated samples of Lippia sp. (oregano) from General Cepeda, Ramos Arizpe, Parras de la Fuente and Matamoros in Coahuila State were analyzed. The molecular description was made through Random Amplified Polymorphic DNA (RAPD) and Simple Sequences Repeated (SSR). In addition, the quantity and quality of essential oils was determined by means of the drag technique by steam and high resolution liquid chromatography. RAPD and SSR markers were able to detect a high level of polymorphism in oregano. However, it was not possible to differentiate the whole assessed collections as well as to gather them in regard to location. In contrast, it was determined that the genetic diversity was mild (47%) and higher among populations. Some samples showed high values of carvacrol while thymol was very heterogeneous among populations. It is concluded that there is enough variation in ...
Some biochemical basis for tolerance of bean cultivars to low nutrient conditions are discussed. ... more Some biochemical basis for tolerance of bean cultivars to low nutrient conditions are discussed. Two tolerant bean cultivars (Flor de Mayo & Laguna) under low nutrient stress accumulated more proline than sensitive varieties (Garbancillo & Pinamerpa). A 38 kDa protein reached higher concentrations in the tolerant cultivars. Laguna and Pinamerpa contained more chlorophyll than the other varieties. It is suggested that proline accumulation and the specific protein may be involved in the adaptation to marginal nutrient situations.
The paper describes diverse aspects of the floral biology including development of floral buds, a... more The paper describes diverse aspects of the floral biology including development of floral buds, anthesis, the sequential process of microsporogenesis. Different stages of fruit development involving color changes during the process of maturity are also discribed.
Para comprobar el efecto de dosis del producto comercial Algaenzims sobre plantulas de 30 dias de... more Para comprobar el efecto de dosis del producto comercial Algaenzims sobre plantulas de 30 dias de dos variedades de sorgo (Rio Bravo y P-83G66) se evaluO: longitud total, longitud de la raiz, longitud de tallo, n´umero de hojas por planta, grosor de tallo, largo y ancho de hoja ligulada, largo y ancho de hojas. Hubo diferencias altamente significativas (P<0.01) y significativas (P<0.05) para longitud de tallo, largo y ancho de hoja, n´umero de hojas por planta y ancho de hoja ligulada. Se analizO tambien, mediante espectrofotometria de emisiOn por plasma, el contenido de Mg, Na, K, Mo, Mn, Ni, Cu, Zn y Cd, tanto en tallo como en raiz, encontrandose diferencias altamente significativas en el contenido de estos minerales tanto entre variedades como entre tipos de tratamientos y variedades.
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