Journal of the Marine Biological Association of the United Kingdom, Sep 9, 2019
The aim of this study was to test the effective separation of shape indices of otoliths of three ... more The aim of this study was to test the effective separation of shape indices of otoliths of three species belonging to the family Sciaenidae before and afterin vitrodigestion. We measured 328 sagittal otoliths and applied six shape indices. Before the experiment, the aspect ratio (otolith height/otolith length%), circularity, ellipticity and relative surface of the sulcus acusticus were suitable for differentiating the species of genera Paralonchurus and Stellifer. Among the species of Stellifer, the aspect ratio and rectangularity were suitable. Otoliths exposed toin vitrodigestion showed no significant differences in their morphometry before and after the experiment. Afterin vitrodigestion, the aspect ratio and circularity were effective in separating Paralonchurus and Stellifer. However, none of the indices used in the present study were efficient to separate otoliths of congeneric species afterin vitrodigestion.
This study assesses the use of stable isotopes (δ13C and δ15N) and trace elements in gills (G), l... more This study assesses the use of stable isotopes (δ13C and δ15N) and trace elements in gills (G), liver (L), and muscle (M) of the most commercially important migratory fish from the La Plata Basin, Prochilodus lineatus, as habitat proxies. With this purpose, fish and sediment (S) samples from the Paraná River and Río de la Plata Estuary were analyzed. Results showed differences in the stable isotopic ratios in fish tissue and sediments between the river and the estuary. In particular, δ15N values of the estuary samples were significantly lower (G = 7.38‰; L = 6.60‰; M = 8.61‰ and S = 4.36‰) than those from the river (G = 11.30–11.53‰; L = 11.03–12.35‰; M = 11.27–11.31‰, and S = 6.78–7.72‰), which could denote a shift in the isotopic baseline between these environments. Quadratic discriminant analysis effectively discriminates between sampling sites with high classification rates (L = 97.5%; G = 90%, and M = 82.5%), suggesting that combining stable isotopes and trace elements is useful to discriminate between fish populations. The liver was the most suitable tissue as a habitat indicator, using mainly δ15N, δ13C, Cr, and Hg as natural markers, and capable of discriminating between sites with a relatively high geographic resolution. Due to the spatial isotopic heterogeneity found in the La Plata Basin, these natural markers could be useful for studying other aquatic organisms' habitat use.
Se plantea el objetivo de identificar y caracterizar habitats utilizados por juveniles de Mugil c... more Se plantea el objetivo de identificar y caracterizar habitats utilizados por juveniles de Mugil cephalus en la Comunidad Valenciana, mediante el estudio de la microquimica del otolito sagitta.
Submission article platform - Latin American Journal of Aquatic Research, May 22, 2017
This study aimed to describe the displacements of anchovy ( Anchoa tricolor ) in a subtropical es... more This study aimed to describe the displacements of anchovy ( Anchoa tricolor ) in a subtropical estuary in South America, using the otolith microchemistry. We analyzed 198 specimens of A. tricolor in the Paranagua Estuarine Complex (Brazil) and data of salinity and rainfall. The biological and environmental data were grouped into sectors (internal, intermediate and external) and periods (early and late rainy and dry periods). The concentrations of Ca, Ba and Sr were determined using an ICP-OES. The Sr: Ca ratio indicated the existence of two groups of probably different origins that use the estuary at the same time but occupy different sectors of the estuary. In the other periods and sectors, we observed that part of the A. tricolor population used the estuary for feeding and growth, with an increase in the Sr: Ca ratio. The Ba: Ca ratio did not differ groups of use in the estuary, but rather, revealed low values suggesting that this species does not use freshwater environments. However, this ratio showed a high variation in the rainy period, indicating greater availability of barium in the estuarine regions affected by river discharge. Considering our results, it is possible to classify A. tricolor as an estuarine migrant species that uses estuaries for feeding and growth. Also, Sr: Ca and Ba: Ca ratios can be indicative of spatial and temporal displacement, respectively.
This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics... more This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contac
Journal of the Marine Biological Association of the United Kingdom
Otoliths are an excellent tool in studies on ecological connectivity of fish species populations.... more Otoliths are an excellent tool in studies on ecological connectivity of fish species populations. Opsanus beta is an invasive species introduced on the Brazilian coast, but not native from the Gulf of Mexico. The present study aimed to compare the otolith contours of specimens collected in Mexico (Celestún, CEL) and in two Brazilian estuaries (Santos Bay, STB, and Paranaguá Estuarine Complex, PEC). In the laboratory, 99 otoliths were extracted, photographed and compared using wavelet analysis. The otolith contours varied between sites (39 from CEL, 26 from STB and 34 from PEC). The linear discriminant analysis correctly reclassified 87.9% of otoliths by sites, with the best reclassifications in the CEL (97.36%), followed by PEC (88.23%) and SBT (73.07%). MANOVA showed significant differences in otolith contours between sites (F = 5.37; P < 0.005). The otolith contour from CEL was significantly different from those from the PEC and SBT. However, the otolith contour of the two Braz...
Métodos de estudios con otolitos: principios y aplicaciones, 2015
El sentido de elaborar este libro surgió de la necesidad de brindarles a los alumnos universitari... more El sentido de elaborar este libro surgió de la necesidad de brindarles a los alumnos universitarios de grado y posgrado, interesados en la aplicación de los otolitos en las ciencias ambientales e agrarias, una herramienta de consulta útil y ágil que sea su primera aproximación al estudio de estas estructuras. Este libro es el resultado del esfuerzo de los coordinadores (Dra. Alejandra Volpedo por Argentina y el Dr. Henry Spach por Brasil) y de los profesores participantes del Proyecto CAFP-BA 043/13 Área de Producción Animal: Pesquerías, del Programa Centros Asociados para el Fortalecimiento de Posgrados Brasil-Argentina (CAFP-BA); quienes a través de los cursos dictados en ambos países han detectado la necesidad de contar en la región con una obra que pudiera reunir los conceptos y metodologías más importantes en el estudio de los otolitos. Esta obra bilingüe, reúne los diferentes aspectos del estudio de los otolitos los cuales están estructurados en capítulos. Dichos capítulos están desarrollados por destacados especialistas a nivel regional e internacional. La realización de este libro fue posible gracias al apoyo de Programa Centros Asociados para el Fortalecimiento de Posgrados Brasil-Argentina (CAFP-BA), a la Secretaría de Políticas Universitarias (SPU) del Ministerio de Educación y a CAPES, así como a la Facultad de Ciencias Veterinarias de la Universidad de Buenos Aires, al Instituto de Investigaciones en Producción Animal (INPA-UBA-CONICET), al Centro de Estudios del Mar y Departamento de Biodiversidad de la Universidad Federal de Paraná (UFPR).Fil: Avigliano, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaFil: Volpedo, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentin
Strontium isotopes (87Sr/86Sr) were evaluated as a potential method for studying the geographical... more Strontium isotopes (87Sr/86Sr) were evaluated as a potential method for studying the geographical origin of populations and movements patterns of migratory fish from the La Plata Basin (Paraná, Uruguay and Bermejo Rivers and Río de la Plata Estuary, South America). Surface water samples were collected at 43 sites during austral summer and winter (2018) while, Surubí (Pseudoplatystoma corruscans), Patí (Luciopimelodus pati), dorado (Salminus brasiliensis) and sábalo (Prochilodus lineatus) fishes were collected at seven locations. Water 87Sr/86Sr ratio was analysed by MC‐ICP‐MS (N = 74) and otolith core‐to‐edge 87Sr/86Sr transects (N = 50) were measured by LAfs‐MC‐ICP‐MS. Several water bodies presented significantly different (p < 0.05) water 87Sr/86Sr values. A ~ 1:1 relationship was found between 87Sr/86Sr measured in edge otolith and water. Data provide novel perspectives about migratory behaviour for all species, such as potential cross‐border migrations between countries of mo...
Trace element:Ca (Ba:Ca, Mg:Ca, Mn:Ca and Sr:Ca) ratios in water and last growth intervals (outer... more Trace element:Ca (Ba:Ca, Mg:Ca, Mn:Ca and Sr:Ca) ratios in water and last growth intervals (outer 45 µm) of dorsal spine and otolith edges from Genidens barbus collected in 3 study areas (Brazil, Argentina−Uruguay and Patagonia) were compared to determine whether the spine can provide a non-lethal natural marker alternative to otoliths for this endangered species. We found an association between calcified structures and the availability of some element:Ca ratios in water. Among the measured element:Ca ratios, a strong correlation in Ba:Ca between outer edges of fin spines and otoliths was found (r 2 = 0.87, p = 0.0001), whereas Mg:Ca, Mn:Ca and Sr:Ca ratios were weakly correlated between structures (0.09 < r 2 < 0.20, 0.0006 < p < 0.02). Several ratios were significantly different among sites for otolith and spine edge (p < 0.05). Permutational multivariate ANOVA (p < 0.05) and quadratic discriminant analysis (QDA) proved highly effective for characterizing differences in otolith and spine edge compositions between sampling sites (mean classification rates: 84.2 and 90.1% for otolith and spine edge, respectively), suggesting that both structures can be used as habitat markers. To identify the possible contribution of multiple stocks to the different areas, we performed QDA for the complete last year of a fish’s life. Classification rates were high for both structures, averaging 79.6 and 81.2% for otolith and spine, respectively, suggesting the existence of new stocks in Patagonian waters. Spine chemistry seems to be an acceptable non-lethal advantage over otoliths to study different biological aspects of catfish.Fil: Avigliano, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaFil: Maichak del Carvalho, Barbara. Universidade Tecnologia Federal do Parana; BrasilFil: Miller, Nathan. University of Texas at Austin; Estados UnidosFil: Córdoba Gironde, Sofía. Universidad Nacional de Río Negro; ArgentinaFil: Tombari, Andrea Diana. Universidad Nacional de Río Negro; ArgentinaFil: Limburg, Karin. State University of New York; Estados UnidosFil: Volpedo, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentin
Journal of the Marine Biological Association of the United Kingdom, Sep 9, 2019
The aim of this study was to test the effective separation of shape indices of otoliths of three ... more The aim of this study was to test the effective separation of shape indices of otoliths of three species belonging to the family Sciaenidae before and afterin vitrodigestion. We measured 328 sagittal otoliths and applied six shape indices. Before the experiment, the aspect ratio (otolith height/otolith length%), circularity, ellipticity and relative surface of the sulcus acusticus were suitable for differentiating the species of genera Paralonchurus and Stellifer. Among the species of Stellifer, the aspect ratio and rectangularity were suitable. Otoliths exposed toin vitrodigestion showed no significant differences in their morphometry before and after the experiment. Afterin vitrodigestion, the aspect ratio and circularity were effective in separating Paralonchurus and Stellifer. However, none of the indices used in the present study were efficient to separate otoliths of congeneric species afterin vitrodigestion.
This study assesses the use of stable isotopes (δ13C and δ15N) and trace elements in gills (G), l... more This study assesses the use of stable isotopes (δ13C and δ15N) and trace elements in gills (G), liver (L), and muscle (M) of the most commercially important migratory fish from the La Plata Basin, Prochilodus lineatus, as habitat proxies. With this purpose, fish and sediment (S) samples from the Paraná River and Río de la Plata Estuary were analyzed. Results showed differences in the stable isotopic ratios in fish tissue and sediments between the river and the estuary. In particular, δ15N values of the estuary samples were significantly lower (G = 7.38‰; L = 6.60‰; M = 8.61‰ and S = 4.36‰) than those from the river (G = 11.30–11.53‰; L = 11.03–12.35‰; M = 11.27–11.31‰, and S = 6.78–7.72‰), which could denote a shift in the isotopic baseline between these environments. Quadratic discriminant analysis effectively discriminates between sampling sites with high classification rates (L = 97.5%; G = 90%, and M = 82.5%), suggesting that combining stable isotopes and trace elements is useful to discriminate between fish populations. The liver was the most suitable tissue as a habitat indicator, using mainly δ15N, δ13C, Cr, and Hg as natural markers, and capable of discriminating between sites with a relatively high geographic resolution. Due to the spatial isotopic heterogeneity found in the La Plata Basin, these natural markers could be useful for studying other aquatic organisms' habitat use.
Se plantea el objetivo de identificar y caracterizar habitats utilizados por juveniles de Mugil c... more Se plantea el objetivo de identificar y caracterizar habitats utilizados por juveniles de Mugil cephalus en la Comunidad Valenciana, mediante el estudio de la microquimica del otolito sagitta.
Submission article platform - Latin American Journal of Aquatic Research, May 22, 2017
This study aimed to describe the displacements of anchovy ( Anchoa tricolor ) in a subtropical es... more This study aimed to describe the displacements of anchovy ( Anchoa tricolor ) in a subtropical estuary in South America, using the otolith microchemistry. We analyzed 198 specimens of A. tricolor in the Paranagua Estuarine Complex (Brazil) and data of salinity and rainfall. The biological and environmental data were grouped into sectors (internal, intermediate and external) and periods (early and late rainy and dry periods). The concentrations of Ca, Ba and Sr were determined using an ICP-OES. The Sr: Ca ratio indicated the existence of two groups of probably different origins that use the estuary at the same time but occupy different sectors of the estuary. In the other periods and sectors, we observed that part of the A. tricolor population used the estuary for feeding and growth, with an increase in the Sr: Ca ratio. The Ba: Ca ratio did not differ groups of use in the estuary, but rather, revealed low values suggesting that this species does not use freshwater environments. However, this ratio showed a high variation in the rainy period, indicating greater availability of barium in the estuarine regions affected by river discharge. Considering our results, it is possible to classify A. tricolor as an estuarine migrant species that uses estuaries for feeding and growth. Also, Sr: Ca and Ba: Ca ratios can be indicative of spatial and temporal displacement, respectively.
This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics... more This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contac
Journal of the Marine Biological Association of the United Kingdom
Otoliths are an excellent tool in studies on ecological connectivity of fish species populations.... more Otoliths are an excellent tool in studies on ecological connectivity of fish species populations. Opsanus beta is an invasive species introduced on the Brazilian coast, but not native from the Gulf of Mexico. The present study aimed to compare the otolith contours of specimens collected in Mexico (Celestún, CEL) and in two Brazilian estuaries (Santos Bay, STB, and Paranaguá Estuarine Complex, PEC). In the laboratory, 99 otoliths were extracted, photographed and compared using wavelet analysis. The otolith contours varied between sites (39 from CEL, 26 from STB and 34 from PEC). The linear discriminant analysis correctly reclassified 87.9% of otoliths by sites, with the best reclassifications in the CEL (97.36%), followed by PEC (88.23%) and SBT (73.07%). MANOVA showed significant differences in otolith contours between sites (F = 5.37; P < 0.005). The otolith contour from CEL was significantly different from those from the PEC and SBT. However, the otolith contour of the two Braz...
Métodos de estudios con otolitos: principios y aplicaciones, 2015
El sentido de elaborar este libro surgió de la necesidad de brindarles a los alumnos universitari... more El sentido de elaborar este libro surgió de la necesidad de brindarles a los alumnos universitarios de grado y posgrado, interesados en la aplicación de los otolitos en las ciencias ambientales e agrarias, una herramienta de consulta útil y ágil que sea su primera aproximación al estudio de estas estructuras. Este libro es el resultado del esfuerzo de los coordinadores (Dra. Alejandra Volpedo por Argentina y el Dr. Henry Spach por Brasil) y de los profesores participantes del Proyecto CAFP-BA 043/13 Área de Producción Animal: Pesquerías, del Programa Centros Asociados para el Fortalecimiento de Posgrados Brasil-Argentina (CAFP-BA); quienes a través de los cursos dictados en ambos países han detectado la necesidad de contar en la región con una obra que pudiera reunir los conceptos y metodologías más importantes en el estudio de los otolitos. Esta obra bilingüe, reúne los diferentes aspectos del estudio de los otolitos los cuales están estructurados en capítulos. Dichos capítulos están desarrollados por destacados especialistas a nivel regional e internacional. La realización de este libro fue posible gracias al apoyo de Programa Centros Asociados para el Fortalecimiento de Posgrados Brasil-Argentina (CAFP-BA), a la Secretaría de Políticas Universitarias (SPU) del Ministerio de Educación y a CAPES, así como a la Facultad de Ciencias Veterinarias de la Universidad de Buenos Aires, al Instituto de Investigaciones en Producción Animal (INPA-UBA-CONICET), al Centro de Estudios del Mar y Departamento de Biodiversidad de la Universidad Federal de Paraná (UFPR).Fil: Avigliano, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaFil: Volpedo, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentin
Strontium isotopes (87Sr/86Sr) were evaluated as a potential method for studying the geographical... more Strontium isotopes (87Sr/86Sr) were evaluated as a potential method for studying the geographical origin of populations and movements patterns of migratory fish from the La Plata Basin (Paraná, Uruguay and Bermejo Rivers and Río de la Plata Estuary, South America). Surface water samples were collected at 43 sites during austral summer and winter (2018) while, Surubí (Pseudoplatystoma corruscans), Patí (Luciopimelodus pati), dorado (Salminus brasiliensis) and sábalo (Prochilodus lineatus) fishes were collected at seven locations. Water 87Sr/86Sr ratio was analysed by MC‐ICP‐MS (N = 74) and otolith core‐to‐edge 87Sr/86Sr transects (N = 50) were measured by LAfs‐MC‐ICP‐MS. Several water bodies presented significantly different (p < 0.05) water 87Sr/86Sr values. A ~ 1:1 relationship was found between 87Sr/86Sr measured in edge otolith and water. Data provide novel perspectives about migratory behaviour for all species, such as potential cross‐border migrations between countries of mo...
Trace element:Ca (Ba:Ca, Mg:Ca, Mn:Ca and Sr:Ca) ratios in water and last growth intervals (outer... more Trace element:Ca (Ba:Ca, Mg:Ca, Mn:Ca and Sr:Ca) ratios in water and last growth intervals (outer 45 µm) of dorsal spine and otolith edges from Genidens barbus collected in 3 study areas (Brazil, Argentina−Uruguay and Patagonia) were compared to determine whether the spine can provide a non-lethal natural marker alternative to otoliths for this endangered species. We found an association between calcified structures and the availability of some element:Ca ratios in water. Among the measured element:Ca ratios, a strong correlation in Ba:Ca between outer edges of fin spines and otoliths was found (r 2 = 0.87, p = 0.0001), whereas Mg:Ca, Mn:Ca and Sr:Ca ratios were weakly correlated between structures (0.09 < r 2 < 0.20, 0.0006 < p < 0.02). Several ratios were significantly different among sites for otolith and spine edge (p < 0.05). Permutational multivariate ANOVA (p < 0.05) and quadratic discriminant analysis (QDA) proved highly effective for characterizing differences in otolith and spine edge compositions between sampling sites (mean classification rates: 84.2 and 90.1% for otolith and spine edge, respectively), suggesting that both structures can be used as habitat markers. To identify the possible contribution of multiple stocks to the different areas, we performed QDA for the complete last year of a fish’s life. Classification rates were high for both structures, averaging 79.6 and 81.2% for otolith and spine, respectively, suggesting the existence of new stocks in Patagonian waters. Spine chemistry seems to be an acceptable non-lethal advantage over otoliths to study different biological aspects of catfish.Fil: Avigliano, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaFil: Maichak del Carvalho, Barbara. Universidade Tecnologia Federal do Parana; BrasilFil: Miller, Nathan. University of Texas at Austin; Estados UnidosFil: Córdoba Gironde, Sofía. Universidad Nacional de Río Negro; ArgentinaFil: Tombari, Andrea Diana. Universidad Nacional de Río Negro; ArgentinaFil: Limburg, Karin. State University of New York; Estados UnidosFil: Volpedo, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentin
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