Fluorescent microscopy and flow cytometry are widely used tools in biomedical sciences. Cost-effe... more Fluorescent microscopy and flow cytometry are widely used tools in biomedical sciences. Cost-effective translation of these technologies to remote and resource-limited environments could create new opportunities especially for telemedicine applications. Toward this direction, here we demonstrate the integration of imaging cytometry and fluorescent microscopy on a cell phone using a compact, lightweight, and cost-effective optofluidic attachment.
We demonstrate a field-portable upright and inverted microscope that can image specimens in both ... more We demonstrate a field-portable upright and inverted microscope that can image specimens in both reflection and transmission modes. This compact and cost-effective dual-mode microscope weighs only $135 grams (< 4.8 ounces) and utilizes a simple light emitting diode (LED) to illuminate the sample of interest using a beam-splitter cube that is positioned above the object plane.
Optical sectioning of biological specimens provides detailed volumetric information regarding the... more Optical sectioning of biological specimens provides detailed volumetric information regarding their internal structure. To provide a complementary approach to existing three-dimensional (3D) microscopy modalities, we have recently demonstrated lensfree optical tomography that offers high-throughput imaging within a compact and simple platform.
Abstract On-chip lensless imaging in general aims to replace bulky lens-based optical microscopes... more Abstract On-chip lensless imaging in general aims to replace bulky lens-based optical microscopes with simpler and more compact designs, especially for high-throughput screening applications. This emerging technology platform has the potential to eliminate the need for bulky and/or costly optical components through the help of novel theories and digital reconstruction algorithms.
The Solid-State, Heat-Capacity Laser (SSHCL) program at Lawrence Livermore National Laboratory is... more The Solid-State, Heat-Capacity Laser (SSHCL) program at Lawrence Livermore National Laboratory is a multigeneration laser development effort scalable to the megawatt power levels. Wavefront quality is a driving metric of its performance. A deformable mirror with over 100 degrees of freedom situated within the cavity is used to correct both the static and dynamic aberrations sensed with a Shack-Hartmann wavefront sensor. The laser geometry is an unstable, confocal resonator with a clear aperture of 10 cm x 10 cm.
The combination of microstructured fiber designs and improving preform fabrication techniques is ... more The combination of microstructured fiber designs and improving preform fabrication techniques is allowing rapid progress in the development of new classes of non-silica fibers. Recent progress in the fabrication of soft glass and polymer microstructured preforms and fibers will be described. Emerging soft glass fibers are enabling new fiber operating regimes including efficient supercontinuum generation and extreme nonlinearity, and recent results in will be reviewed.
For next generation fiber to the home (FTTH) system, we are asked to use inexpensive optical devi... more For next generation fiber to the home (FTTH) system, we are asked to use inexpensive optical devices. For that the use of soft-lithography instead of standard photolithography and dry etching technologies is attractive because cost saving optical device can be realized. Polymerization using multi-photon absorption of materials is also a good method for optical waveguide fabrication. Using these processes, we can fabricate the waveguide and connect it with optical fibers at the same time.
Optical microscopy is an indispensable tool in science and medicine. However, the size and cost o... more Optical microscopy is an indispensable tool in science and medicine. However, the size and cost of conventional optical microscopes limit their use in rugged field-settings or in remote areas to assist in tasks such as medical tests, disease diagnostics, or monitoring of water quality.
The separation of biological cells by filtration through microstructured constrictions is limited... more The separation of biological cells by filtration through microstructured constrictions is limited by unpredictable variations of the filter hydrodynamic resistance as cells accumulate in the microstructure. Applying a reverse flow to unclog the filter will undo the separation and reduce filter selectivity because of the reversibility of low-Reynolds number flow. We introduce a microfluidic structural ratchet mechanism to separate cells using oscillatory flow.
We demonstrate a personalized food allergen testing platform, termed iTube, running on a cellphon... more We demonstrate a personalized food allergen testing platform, termed iTube, running on a cellphone that images and automatically analyses colorimetric assays performed in test tubes toward sensitive and specific detection of allergens in food samples.
Fluorescent microscopy and flow cytometry are widely used tools in biomedical sciences. Cost-effe... more Fluorescent microscopy and flow cytometry are widely used tools in biomedical sciences. Cost-effective translation of these technologies to remote and resource-limited environments could create new opportunities especially for telemedicine applications. Toward this direction, here we demonstrate the integration of imaging cytometry and fluorescent microscopy on a cell phone using a compact, lightweight, and cost-effective optofluidic attachment.
We demonstrate a field-portable upright and inverted microscope that can image specimens in both ... more We demonstrate a field-portable upright and inverted microscope that can image specimens in both reflection and transmission modes. This compact and cost-effective dual-mode microscope weighs only $135 grams (< 4.8 ounces) and utilizes a simple light emitting diode (LED) to illuminate the sample of interest using a beam-splitter cube that is positioned above the object plane.
Optical sectioning of biological specimens provides detailed volumetric information regarding the... more Optical sectioning of biological specimens provides detailed volumetric information regarding their internal structure. To provide a complementary approach to existing three-dimensional (3D) microscopy modalities, we have recently demonstrated lensfree optical tomography that offers high-throughput imaging within a compact and simple platform.
Abstract On-chip lensless imaging in general aims to replace bulky lens-based optical microscopes... more Abstract On-chip lensless imaging in general aims to replace bulky lens-based optical microscopes with simpler and more compact designs, especially for high-throughput screening applications. This emerging technology platform has the potential to eliminate the need for bulky and/or costly optical components through the help of novel theories and digital reconstruction algorithms.
The Solid-State, Heat-Capacity Laser (SSHCL) program at Lawrence Livermore National Laboratory is... more The Solid-State, Heat-Capacity Laser (SSHCL) program at Lawrence Livermore National Laboratory is a multigeneration laser development effort scalable to the megawatt power levels. Wavefront quality is a driving metric of its performance. A deformable mirror with over 100 degrees of freedom situated within the cavity is used to correct both the static and dynamic aberrations sensed with a Shack-Hartmann wavefront sensor. The laser geometry is an unstable, confocal resonator with a clear aperture of 10 cm x 10 cm.
The combination of microstructured fiber designs and improving preform fabrication techniques is ... more The combination of microstructured fiber designs and improving preform fabrication techniques is allowing rapid progress in the development of new classes of non-silica fibers. Recent progress in the fabrication of soft glass and polymer microstructured preforms and fibers will be described. Emerging soft glass fibers are enabling new fiber operating regimes including efficient supercontinuum generation and extreme nonlinearity, and recent results in will be reviewed.
For next generation fiber to the home (FTTH) system, we are asked to use inexpensive optical devi... more For next generation fiber to the home (FTTH) system, we are asked to use inexpensive optical devices. For that the use of soft-lithography instead of standard photolithography and dry etching technologies is attractive because cost saving optical device can be realized. Polymerization using multi-photon absorption of materials is also a good method for optical waveguide fabrication. Using these processes, we can fabricate the waveguide and connect it with optical fibers at the same time.
Optical microscopy is an indispensable tool in science and medicine. However, the size and cost o... more Optical microscopy is an indispensable tool in science and medicine. However, the size and cost of conventional optical microscopes limit their use in rugged field-settings or in remote areas to assist in tasks such as medical tests, disease diagnostics, or monitoring of water quality.
The separation of biological cells by filtration through microstructured constrictions is limited... more The separation of biological cells by filtration through microstructured constrictions is limited by unpredictable variations of the filter hydrodynamic resistance as cells accumulate in the microstructure. Applying a reverse flow to unclog the filter will undo the separation and reduce filter selectivity because of the reversibility of low-Reynolds number flow. We introduce a microfluidic structural ratchet mechanism to separate cells using oscillatory flow.
We demonstrate a personalized food allergen testing platform, termed iTube, running on a cellphon... more We demonstrate a personalized food allergen testing platform, termed iTube, running on a cellphone that images and automatically analyses colorimetric assays performed in test tubes toward sensitive and specific detection of allergens in food samples.
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