A laparoscopy technique was employed to sterilize five adult male dogs. The animals were sedated ... more A laparoscopy technique was employed to sterilize five adult male dogs. The animals were sedated with a combination of medetomidine and buprenorphine intravenously and a fragment of the ductus deferens, about 2 cm in length, was removed after having been cauterized and cut, using atraumatic forceps and a pair of scissors connected to an electrocautery unit. The procedure was easy, quick and safe. The animals had no complications after the intervention and made a good recovery.
In this study, the fluorescent lipid dye Nile Red, was used to demonstrate that the lipid content... more In this study, the fluorescent lipid dye Nile Red, was used to demonstrate that the lipid content of immature bovine oocytes is correlated with the morphological appearance of the ooplasm. Oocytes with a uniform dark cytoplasm contained significantly more intracellular lipids in lipid droplets compared with oocytes with a granulated or pale cytoplasm (p < 0.05). Furthermore, this lipid-analysing technique was applied for the first time on single bovine in vitro embryos, showing a significant increase of the lipid content in lipid droplets after culture in the presence of serum (p < 0.05).
A laparoscopy technique was employed to sterilize five adult male dogs. The animals were sedated ... more A laparoscopy technique was employed to sterilize five adult male dogs. The animals were sedated with a combination of medetomidine and buprenorphine intravenously and a fragment of the ductus deferens, about 2 cm in length, was removed after having been cauterized and cut, using atraumatic forceps and a pair of scissors connected to an electrocautery unit. The procedure was easy, quick and safe. The animals had no complications after the intervention and made a good recovery.
In this study, the fluorescent lipid dye Nile Red, was used to demonstrate that the lipid content... more In this study, the fluorescent lipid dye Nile Red, was used to demonstrate that the lipid content of immature bovine oocytes is correlated with the morphological appearance of the ooplasm. Oocytes with a uniform dark cytoplasm contained significantly more intracellular lipids in lipid droplets compared with oocytes with a granulated or pale cytoplasm (p < 0.05). Furthermore, this lipid-analysing technique was applied for the first time on single bovine in vitro embryos, showing a significant increase of the lipid content in lipid droplets after culture in the presence of serum (p < 0.05).
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Papers by Isabelle Donnay