ObjectivesThe present study was conducted to detect the occurrence of β-lactamase and biofilm-pro... more ObjectivesThe present study was conducted to detect the occurrence of β-lactamase and biofilm-producing Escherichia coli, Salmonella, and Klebsiella in broilers and native fowl reared in the Andaman and Nicobar Islands, India. The study also included molecular docking experiments to confirm the nature of the catalytic domains found in the β-lactamase variants obtained and to reveal the clonal relationship of the isolates with human clinical strains from the database.Materials and methodsA total of 199 cloacal swabs were collected from five poultry breeds/varieties (broiler, Vanraja, Desi, Nicobari, and layer) in three districts of the Andaman and Nicobar Islands. E. coli, Salmonella enterica, and Klebsiella pneumoniae were isolated by standard techniques and confirmed by PCR. Phenotypical β-lactamase producers were identified by a double-disc test. The genes (blaCTX, blaSHV, blaTEM, and blaAmpC) were screened, and selected sequences of β-lactamase variants were submitted to DDBJ. Ho...
Wastewater-based testing (WBT) for SARS-CoV-2 has rapidly expanded over the past three years due ... more Wastewater-based testing (WBT) for SARS-CoV-2 has rapidly expanded over the past three years due to its ability to provide a comprehensive measurement of disease prevalence independent of clinical testing. The development and simultaneous application of the field blurred the boundary between measuring biomarkers for research activities and for pursuit of public health goals, both areas with well-established ethical frameworks. Currently, WBT practitioners do not employ a standardized ethical review process (or associated data management safeguards), introducing the potential for adverse outcomes for WBT professionals and community members. To address this deficiency, an interdisciplinary group developed a framework for a structured ethical review of WBT. The workshop employed a consensus approach to create this framework as a set of 11-questions derived from primarily public health guidance because of the common exemption of wastewater samples to human subject research consideration...
Wastewater surveillance has been widely used to track the prevalence of SARS-CoV-2 in communities... more Wastewater surveillance has been widely used to track the prevalence of SARS-CoV-2 in communities. Although some studies have investigated the decay of SARS-CoV-2 RNA in wastewater, understanding about its fate during wastewater transport in real sewers is still limited. This study aims to assess the impact of sewer biofilms on the dynamics of SARS-CoV-2 RNA concentration in naturally contaminated real wastewater (raw influent wastewater without extra SARS-CoV-2 virus/gene seeding) using a simulated laboratory-scale sewer system. The results indicated that, with the sewer biofilms, a 90% concentration reduction of the SARS-CoV-2 RNA was observed within 2 h both in wastewater of gravity (GS, gravity-driven sewers) and rising main (RM, pressurized sewers) sewer reactors. In contrast, the 90% reduction time was 8–26 h in control reactors without biofilms. The concentration reduction of SARS-CoV-2 RNA in wastewater was significantly more in the presence of sewer biofilms. In addition, a...
The effective detection of viruses in aircraft wastewater is crucial to establish surveillance pr... more The effective detection of viruses in aircraft wastewater is crucial to establish surveillance programs for monitoring virus spread via aircraft passengers. This study aimed to compare the performance of two virus concentration workflows, adsorption-extraction (AE) and Nanotrap® Microbiome A Particles (NMAP), in detecting the prevalence and concentrations of 15 endogenous viruses in aircraft lavatory wastewater samples. The viruses tested include two indicator viruses, four enteric viruses, and nine respiratory viruses. The results showed that cross-assembly phage (crAssphage), human polyomavirus (HPyV), rhinovirus A (RhV A), and rhinovirus B (RhV B) were detected in all wastewater samples using both workflows. However, enterovirus (EV), human norovirus (HNoV GII), human adenovirus (HAdV), bocavirus (BoV), parechovirus (PeV), epstein-barr virus (EBV), influenza A virus (IAV), and respiratory syncytial virus B (RsV B) were infrequently detected by both workflows, and hepatitis A viru...
Coronavirus disease 2019 (COVID-19) has spread across the globe producing hundreds of thousands o... more Coronavirus disease 2019 (COVID-19) has spread across the globe producing hundreds of thousands of deaths, shutting down economies, closing borders and causing havoc on an unprecedented scale. Its potent effects have earned the attention of researchers in different fields worldwide. Among them, authors from different countries have published numerous research articles based on the environmental concepts of COVID-19. The environment is considered an essential receptor in the COVID-19 pandemic, and it is academically significant to look into publications to follow the pathway of hot topics of research and upcoming trends in studies. Reviewing the literature can therefore provide valuable information regarding the strengths and weaknesses in facing the COVID-19 pandemic, considering the environmental viewpoint. The present study categorizes the understanding caused by environmental and COVID-19-related published papers in the Scopus metadata from 2020 to 2021. VOSviewer is a promising ...
The emergence of new variants of SARS-CoV-2 associated with varying infectivity, pathogenicity, d... more The emergence of new variants of SARS-CoV-2 associated with varying infectivity, pathogenicity, diagnosis, and effectiveness against treatments challenged the overall management of the COVID-19 pandemic. Wastewater surveillance (WWS), i.e., monitoring COVID-19 infections in communities through detecting viruses in wastewater, was applied to track the emergence and spread of SARS-CoV-2 variants globally. However, there is a lack of comprehensive understanding of the use and effectiveness of WWS for new SARS-CoV-2 variants. Here we systematically reviewed published articles reporting monitoring of different SARS-CoV-2 variants in wastewater by following the PRISMA guidelines and provided the current state of the art of this study area. A total of 80 WWS studies were found that reported different monitoring variants of SARS-CoV-2 until November 2022. Most of these studies (66 out of the total 80, 82.5%) were conducted in Europe and North America, i.e., resource-rich countries. There wa...
International Journal of Environmental Research and Public Health
The emergence of an outbreak of Monkeypox disease (MPXD) is caused by a contagious zoonotic Monke... more The emergence of an outbreak of Monkeypox disease (MPXD) is caused by a contagious zoonotic Monkeypox virus (MPXV) that has spread globally. Yet, there is no study investigating the effect of climatic changes on MPXV transmission. Thus, studies on the changing epidemiology, evolving nature of the virus, and ecological niche are highly paramount. Determination of the role of potential meteorological drivers including temperature, precipitation, relative humidity, dew point, wind speed, and surface pressure is beneficial to understand the MPXD outbreak. This study examines the changes in MPXV cases over time while assessing the meteorological characteristics that could impact these disparities from the onset of the global outbreak. To conduct this data-based research, several well-accepted statistical techniques including Simple Exponential Smoothing (SES), Auto-Regressive Integrated Moving Average (ARIMA), Automatic forecasting time-series model (Prophet), and Autoregressive Integrat...
Infectious diseases caused by antibiotic-resistant bacterial (ARB) pathogens are a serious threat... more Infectious diseases caused by antibiotic-resistant bacterial (ARB) pathogens are a serious threat to human and animal health. The active surveillance of ARB using an integrated one-health approach can help to reduce the emergence and spread of ARB, reduce the associated economic impact, and guide antimicrobial stewardship programs. Wastewater surveillance (WWS) of ARB provides composite samples for a total population, with easy access to the mixed community microbiome. This concept is emerging rapidly, but the clinical utility, sensitivity, and uniformity of WWS of ARB remain poorly understood especially in relation to clinical evidence in sewershed communities. Here, we systematically searched the literature to identify studies that have compared findings from WWS of ARB and antibiotic resistance genes (ARG) with clinical evidence in parallel, thereby evaluating how likely WWS of ARB and ARG can relate to the clinical cases in communities. Initially, 2,235 articles were obtained us...
Highlights • Archaea-specific primers in high-throughput sequencing found archaea reads.• Archaea... more Highlights • Archaea-specific primers in high-throughput sequencing found archaea reads.• Archaea community with DNA-based analysis was different from RNA-based analysis.• Archaea are a natural part of microbiomes in drinking water distribution systems.• Active archaea reads were high in non-disinfected and cold distribution systems.
Wastewater Based Epidemiology (WBE) is an important tool to fight against COVID-19 as it provides... more Wastewater Based Epidemiology (WBE) is an important tool to fight against COVID-19 as it provides insights into the health status of the targeted population from a small single house to a large municipality level in a cost-effective, rapid and non-invasive way. The implementation of WBE-based surveillance could reduce the burden on the public health system, management of pandemics, help make informed decisions and protect public health. In this study, a house of COVID-19 patients was targeted for monitoring the prevalence of SARS-CoV-2 genetic markers in wastewater samples (WS) with clinical specimens (CS) over a period of 30 days. RT-qPCR technique was employed to target nonstructural (ORF1ab) and structural-nucleocapsid (N) protein genes of SARS-CoV-2, according to the validated experimental protocol. Physiological, environmental, and biological parameters were also measured following the American Public Health Association (APHA) standard protocol. SARS-CoV-2 viral shedding in wastewater peaked when the highest number of COVID-19 cases were clinically diagnosed. Throughout the study period, 7450 to 23000 gene copies/1000 mL were detected, where we found 47 percent (57/120) positive samples from WS and 35 percent (128/360) from CS. When the COVID-19 patient number was the lowest (2), the highest CT value (39.4; i.e., lowest copy number) was identified from WS. On the other hand, when the COVID-19 patients were the highest (6), the lowest CT value (25.2 i.e., highest copy numbers) was obtained from WS. An increased signal of the SARS-CoV-2 viral load from the COVID-19 patient signal was found in the WS earlier than in CS. Using customized primer sets in a traditional PCR approach, we confirmed that all SARSCoV-2 variants from CS and WS were Delta variants (B.1.617.2). To our knowledge, this is the first follow-up study to determine a relationship between COVID-19 patients and the discharge of SARS-CoV-2 RNA genetic markers in wastewater from a single house including all family members for clinical sampling from a developing country (Bangladesh) without a proper sewage system. The findings of the study indicate that monitoring the genetic markers of the SARSCoV-2 virus in wastewater could identify COVID-19 cases, which reduces the burden on the public health system in COVID-19 pandemics.
ObjectivesThe present study was conducted to detect the occurrence of β-lactamase and biofilm-pro... more ObjectivesThe present study was conducted to detect the occurrence of β-lactamase and biofilm-producing Escherichia coli, Salmonella, and Klebsiella in broilers and native fowl reared in the Andaman and Nicobar Islands, India. The study also included molecular docking experiments to confirm the nature of the catalytic domains found in the β-lactamase variants obtained and to reveal the clonal relationship of the isolates with human clinical strains from the database.Materials and methodsA total of 199 cloacal swabs were collected from five poultry breeds/varieties (broiler, Vanraja, Desi, Nicobari, and layer) in three districts of the Andaman and Nicobar Islands. E. coli, Salmonella enterica, and Klebsiella pneumoniae were isolated by standard techniques and confirmed by PCR. Phenotypical β-lactamase producers were identified by a double-disc test. The genes (blaCTX, blaSHV, blaTEM, and blaAmpC) were screened, and selected sequences of β-lactamase variants were submitted to DDBJ. Ho...
Wastewater-based testing (WBT) for SARS-CoV-2 has rapidly expanded over the past three years due ... more Wastewater-based testing (WBT) for SARS-CoV-2 has rapidly expanded over the past three years due to its ability to provide a comprehensive measurement of disease prevalence independent of clinical testing. The development and simultaneous application of the field blurred the boundary between measuring biomarkers for research activities and for pursuit of public health goals, both areas with well-established ethical frameworks. Currently, WBT practitioners do not employ a standardized ethical review process (or associated data management safeguards), introducing the potential for adverse outcomes for WBT professionals and community members. To address this deficiency, an interdisciplinary group developed a framework for a structured ethical review of WBT. The workshop employed a consensus approach to create this framework as a set of 11-questions derived from primarily public health guidance because of the common exemption of wastewater samples to human subject research consideration...
Wastewater surveillance has been widely used to track the prevalence of SARS-CoV-2 in communities... more Wastewater surveillance has been widely used to track the prevalence of SARS-CoV-2 in communities. Although some studies have investigated the decay of SARS-CoV-2 RNA in wastewater, understanding about its fate during wastewater transport in real sewers is still limited. This study aims to assess the impact of sewer biofilms on the dynamics of SARS-CoV-2 RNA concentration in naturally contaminated real wastewater (raw influent wastewater without extra SARS-CoV-2 virus/gene seeding) using a simulated laboratory-scale sewer system. The results indicated that, with the sewer biofilms, a 90% concentration reduction of the SARS-CoV-2 RNA was observed within 2 h both in wastewater of gravity (GS, gravity-driven sewers) and rising main (RM, pressurized sewers) sewer reactors. In contrast, the 90% reduction time was 8–26 h in control reactors without biofilms. The concentration reduction of SARS-CoV-2 RNA in wastewater was significantly more in the presence of sewer biofilms. In addition, a...
The effective detection of viruses in aircraft wastewater is crucial to establish surveillance pr... more The effective detection of viruses in aircraft wastewater is crucial to establish surveillance programs for monitoring virus spread via aircraft passengers. This study aimed to compare the performance of two virus concentration workflows, adsorption-extraction (AE) and Nanotrap® Microbiome A Particles (NMAP), in detecting the prevalence and concentrations of 15 endogenous viruses in aircraft lavatory wastewater samples. The viruses tested include two indicator viruses, four enteric viruses, and nine respiratory viruses. The results showed that cross-assembly phage (crAssphage), human polyomavirus (HPyV), rhinovirus A (RhV A), and rhinovirus B (RhV B) were detected in all wastewater samples using both workflows. However, enterovirus (EV), human norovirus (HNoV GII), human adenovirus (HAdV), bocavirus (BoV), parechovirus (PeV), epstein-barr virus (EBV), influenza A virus (IAV), and respiratory syncytial virus B (RsV B) were infrequently detected by both workflows, and hepatitis A viru...
Coronavirus disease 2019 (COVID-19) has spread across the globe producing hundreds of thousands o... more Coronavirus disease 2019 (COVID-19) has spread across the globe producing hundreds of thousands of deaths, shutting down economies, closing borders and causing havoc on an unprecedented scale. Its potent effects have earned the attention of researchers in different fields worldwide. Among them, authors from different countries have published numerous research articles based on the environmental concepts of COVID-19. The environment is considered an essential receptor in the COVID-19 pandemic, and it is academically significant to look into publications to follow the pathway of hot topics of research and upcoming trends in studies. Reviewing the literature can therefore provide valuable information regarding the strengths and weaknesses in facing the COVID-19 pandemic, considering the environmental viewpoint. The present study categorizes the understanding caused by environmental and COVID-19-related published papers in the Scopus metadata from 2020 to 2021. VOSviewer is a promising ...
The emergence of new variants of SARS-CoV-2 associated with varying infectivity, pathogenicity, d... more The emergence of new variants of SARS-CoV-2 associated with varying infectivity, pathogenicity, diagnosis, and effectiveness against treatments challenged the overall management of the COVID-19 pandemic. Wastewater surveillance (WWS), i.e., monitoring COVID-19 infections in communities through detecting viruses in wastewater, was applied to track the emergence and spread of SARS-CoV-2 variants globally. However, there is a lack of comprehensive understanding of the use and effectiveness of WWS for new SARS-CoV-2 variants. Here we systematically reviewed published articles reporting monitoring of different SARS-CoV-2 variants in wastewater by following the PRISMA guidelines and provided the current state of the art of this study area. A total of 80 WWS studies were found that reported different monitoring variants of SARS-CoV-2 until November 2022. Most of these studies (66 out of the total 80, 82.5%) were conducted in Europe and North America, i.e., resource-rich countries. There wa...
International Journal of Environmental Research and Public Health
The emergence of an outbreak of Monkeypox disease (MPXD) is caused by a contagious zoonotic Monke... more The emergence of an outbreak of Monkeypox disease (MPXD) is caused by a contagious zoonotic Monkeypox virus (MPXV) that has spread globally. Yet, there is no study investigating the effect of climatic changes on MPXV transmission. Thus, studies on the changing epidemiology, evolving nature of the virus, and ecological niche are highly paramount. Determination of the role of potential meteorological drivers including temperature, precipitation, relative humidity, dew point, wind speed, and surface pressure is beneficial to understand the MPXD outbreak. This study examines the changes in MPXV cases over time while assessing the meteorological characteristics that could impact these disparities from the onset of the global outbreak. To conduct this data-based research, several well-accepted statistical techniques including Simple Exponential Smoothing (SES), Auto-Regressive Integrated Moving Average (ARIMA), Automatic forecasting time-series model (Prophet), and Autoregressive Integrat...
Infectious diseases caused by antibiotic-resistant bacterial (ARB) pathogens are a serious threat... more Infectious diseases caused by antibiotic-resistant bacterial (ARB) pathogens are a serious threat to human and animal health. The active surveillance of ARB using an integrated one-health approach can help to reduce the emergence and spread of ARB, reduce the associated economic impact, and guide antimicrobial stewardship programs. Wastewater surveillance (WWS) of ARB provides composite samples for a total population, with easy access to the mixed community microbiome. This concept is emerging rapidly, but the clinical utility, sensitivity, and uniformity of WWS of ARB remain poorly understood especially in relation to clinical evidence in sewershed communities. Here, we systematically searched the literature to identify studies that have compared findings from WWS of ARB and antibiotic resistance genes (ARG) with clinical evidence in parallel, thereby evaluating how likely WWS of ARB and ARG can relate to the clinical cases in communities. Initially, 2,235 articles were obtained us...
Highlights • Archaea-specific primers in high-throughput sequencing found archaea reads.• Archaea... more Highlights • Archaea-specific primers in high-throughput sequencing found archaea reads.• Archaea community with DNA-based analysis was different from RNA-based analysis.• Archaea are a natural part of microbiomes in drinking water distribution systems.• Active archaea reads were high in non-disinfected and cold distribution systems.
Wastewater Based Epidemiology (WBE) is an important tool to fight against COVID-19 as it provides... more Wastewater Based Epidemiology (WBE) is an important tool to fight against COVID-19 as it provides insights into the health status of the targeted population from a small single house to a large municipality level in a cost-effective, rapid and non-invasive way. The implementation of WBE-based surveillance could reduce the burden on the public health system, management of pandemics, help make informed decisions and protect public health. In this study, a house of COVID-19 patients was targeted for monitoring the prevalence of SARS-CoV-2 genetic markers in wastewater samples (WS) with clinical specimens (CS) over a period of 30 days. RT-qPCR technique was employed to target nonstructural (ORF1ab) and structural-nucleocapsid (N) protein genes of SARS-CoV-2, according to the validated experimental protocol. Physiological, environmental, and biological parameters were also measured following the American Public Health Association (APHA) standard protocol. SARS-CoV-2 viral shedding in wastewater peaked when the highest number of COVID-19 cases were clinically diagnosed. Throughout the study period, 7450 to 23000 gene copies/1000 mL were detected, where we found 47 percent (57/120) positive samples from WS and 35 percent (128/360) from CS. When the COVID-19 patient number was the lowest (2), the highest CT value (39.4; i.e., lowest copy number) was identified from WS. On the other hand, when the COVID-19 patients were the highest (6), the lowest CT value (25.2 i.e., highest copy numbers) was obtained from WS. An increased signal of the SARS-CoV-2 viral load from the COVID-19 patient signal was found in the WS earlier than in CS. Using customized primer sets in a traditional PCR approach, we confirmed that all SARSCoV-2 variants from CS and WS were Delta variants (B.1.617.2). To our knowledge, this is the first follow-up study to determine a relationship between COVID-19 patients and the discharge of SARS-CoV-2 RNA genetic markers in wastewater from a single house including all family members for clinical sampling from a developing country (Bangladesh) without a proper sewage system. The findings of the study indicate that monitoring the genetic markers of the SARSCoV-2 virus in wastewater could identify COVID-19 cases, which reduces the burden on the public health system in COVID-19 pandemics.
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Papers by ananda tiwari