Production of monoclonal antibodies to C3d usually involves the purification of protein. Our meth... more Production of monoclonal antibodies to C3d usually involves the purification of protein. Our method does not require C3 purification; it relies on attachment of C3b to mouse erythrocytes by activation of alternative pathways and further conversion in C3d. We prepared human complement-coated mouse red cells and sensitized mice of the same strain with our own schedule of immunization and applied the classical methods to obtain a mouse monoclonal antibody. We obtained a clone called BMS-11 which produces a monoclonal antibody of IgM class, to C3d with a title of 1:500000. The monoclonal antibody obtained has shown that it is suitable for use as an antiglobulin reagent.
Objective: Hyperhomocysteinemia has been implicated as a causative factor in intimal hyperplasia ... more Objective: Hyperhomocysteinemia has been implicated as a causative factor in intimal hyperplasia development. The addition of dietary folate in a hyperhomocysteinemia, carotid endarterectomy rat model is postulated to decrease plasma homocysteine levels and, in turn, reduce post–carotid endarterectomy intimal hyperplasia. Methods: Each rat was fed one of six diets: (1) lab chow with no folate (n = 7), (2) lab
International Conference on Electronics, Communications, and Computers, 2007
This paper presents a novel approach for images retrieval form digital collection based on analys... more This paper presents a novel approach for images retrieval form digital collection based on analysis of their low level characteristics such as color and shape that has been applied for classification of the rare specimens of the Lepidoptera (butterflies) in Mexico. The similarity with user's queries which are the images, manual sketches or textual descriptions is computed by comparison of
Production of monoclonal antibodies to C3d usually involves the purification of protein. Our meth... more Production of monoclonal antibodies to C3d usually involves the purification of protein. Our method does not require C3 purification; it relies on attachment of C3b to mouse erythrocytes by activation of alternative pathways and further conversion in C3d. We prepared human complement-coated mouse red cells and sensitized mice of the same strain with our own schedule of immunization and applied the classical methods to obtain a mouse monoclonal antibody. We obtained a clone called BMS-11 which produces a monoclonal antibody of IgM class, to C3d with a title of 1:500000. The monoclonal antibody obtained has shown that it is suitable for use as an antiglobulin reagent.
Objective: Hyperhomocysteinemia has been implicated as a causative factor in intimal hyperplasia ... more Objective: Hyperhomocysteinemia has been implicated as a causative factor in intimal hyperplasia development. The addition of dietary folate in a hyperhomocysteinemia, carotid endarterectomy rat model is postulated to decrease plasma homocysteine levels and, in turn, reduce post–carotid endarterectomy intimal hyperplasia. Methods: Each rat was fed one of six diets: (1) lab chow with no folate (n = 7), (2) lab
International Conference on Electronics, Communications, and Computers, 2007
This paper presents a novel approach for images retrieval form digital collection based on analys... more This paper presents a novel approach for images retrieval form digital collection based on analysis of their low level characteristics such as color and shape that has been applied for classification of the rare specimens of the Lepidoptera (butterflies) in Mexico. The similarity with user's queries which are the images, manual sketches or textual descriptions is computed by comparison of
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