Additional file 6. Subcellular localization of identified proteins. The analysis was performed in... more Additional file 6. Subcellular localization of identified proteins. The analysis was performed in the Blast2Go software. Subcellular localization from identified proteins of Catongo (A) and TSH1188 (B) genotypes at 72HAI. Subcellular localization from Catongo (C) and TSH1188 (D) genotypes at 45DAI.
Total spots identified in the MS/MS. In sequence, mw corresponds to a molecular mass values, the ... more Total spots identified in the MS/MS. In sequence, mw corresponds to a molecular mass values, the isoelectric point pI estimated; number of peptides by MS / MS; Score of sequence, sequence coverage and peptide sequence. All values were calculated by Mascot at http:. // www.matrixscience.com . (XLS 118 kb)
Background: Witches’ broom disease (WBD) of cacao (Theobroma cacao L.), caused by Moniliophthora ... more Background: Witches’ broom disease (WBD) of cacao (Theobroma cacao L.), caused by Moniliophthora perniciosa, is one of the main problems limiting cacao production, thus, the development of cacao genotypes with durable resistance is the main challenge for disease control. Proteomic methods are often used to study the interactions between hosts and pathogens, therefore helping classical plant breeding in the development of resistant genotypes. Here, we compared the proteomic alterations of two cacao genotypes standard for WBD resistance and susceptibility in response to M. perniciosa infection at 72 hours and 45 days of post-inoculation. Results: A total of 554 proteins were identified, 246 in the susceptible and 308 in the resistant genotype. The identified proteins were involved mainly in metabolism, energy, defense and oxidative stress. The resistant genotype showed more expressed proteins as well as more variability of proteins associated to stress and defense, while the susceptib...
Additional file 6. Subcellular localization of identified proteins. The analysis was performed in... more Additional file 6. Subcellular localization of identified proteins. The analysis was performed in the Blast2Go software. Subcellular localization from identified proteins of Catongo (A) and TSH1188 (B) genotypes at 72HAI. Subcellular localization from Catongo (C) and TSH1188 (D) genotypes at 45DAI.
Total spots identified in the MS/MS. In sequence, mw corresponds to a molecular mass values, the ... more Total spots identified in the MS/MS. In sequence, mw corresponds to a molecular mass values, the isoelectric point pI estimated; number of peptides by MS / MS; Score of sequence, sequence coverage and peptide sequence. All values were calculated by Mascot at http:. // www.matrixscience.com . (XLS 118 kb)
Background: Witches’ broom disease (WBD) of cacao (Theobroma cacao L.), caused by Moniliophthora ... more Background: Witches’ broom disease (WBD) of cacao (Theobroma cacao L.), caused by Moniliophthora perniciosa, is one of the main problems limiting cacao production, thus, the development of cacao genotypes with durable resistance is the main challenge for disease control. Proteomic methods are often used to study the interactions between hosts and pathogens, therefore helping classical plant breeding in the development of resistant genotypes. Here, we compared the proteomic alterations of two cacao genotypes standard for WBD resistance and susceptibility in response to M. perniciosa infection at 72 hours and 45 days of post-inoculation. Results: A total of 554 proteins were identified, 246 in the susceptible and 308 in the resistant genotype. The identified proteins were involved mainly in metabolism, energy, defense and oxidative stress. The resistant genotype showed more expressed proteins as well as more variability of proteins associated to stress and defense, while the susceptib...
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