Journal of Essential Oil Bearing Plants, Nov 2, 2019
Abstract To evaluate the antioxidant activity of fourteen essential oils (EOs) isolated from myrt... more Abstract To evaluate the antioxidant activity of fourteen essential oils (EOs) isolated from myrtle (Myrtus communis L.) collected at different regions of Algeria, seven different assays were carried out: ability for scavenging ABTS (2,2-azinobis(3-ethylbenzothiazoline)-6-sulfonic acid), DPPH (2,2-diphenyl-1-picrylhydrazyl), HO (hydroxyl), NO (nitric oxide) free radicals; preventing lipid peroxidation through the thiobarbituric reactive species method, for reducing ferric ions to ferrous ions and chelating activity. The antiproliferative capacity of the EOs on Caco-2 cells were also evaluated using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method. The results showed that the essential oils were mainly constituted by α-pinene, 1,8-cineole, limonene and linalool. The EOs at different concentrations showed moderate to poor antioxidant activity. The sample Mc_Lf_06 was the single one that presented higher antioxidant activity across the four assays (prevention of lipid peroxidation, ability for scavenging the ABTS, NO and hydroxyl radicals). The EOs Mc_Lf_06 and Mc_Lf_10 showed the best antiproliferative activity but only achieving 20 % inhibition after 96 h of incubation for all concentrations tested. Intriguing the sample Mc_Lf_07 that stimulated the proliferation of Caco-2 cells, particularly at lower concentrations (0.1 and 0.2 mg/mL).
International Journal of Food Sciences and Nutrition, Jan 20, 2014
In this study, 17 commercial honey samples from Morocco were analyzed. Four samples did not meet ... more In this study, 17 commercial honey samples from Morocco were analyzed. Four samples did not meet the international physicochemical standards due to high hydroxymethylfurfural content and low diastase activity. Phenol content varied from 163.82 mg gallic acid equivalent (GAE)/kg in citrus honey to 923.70 mg (GAE)/kg in thyme honey from Rachidia; flavonoid content ranged from 4.26 mg quercetin equivalent (QE)/kg in citrus honey to 139.62 mg QE/kg in black cumin honey. Black cumin honey had the highest peroxyl scavenging activity; oregano (from Zaraphyt) and thyme honeys (from Rachidia) had the highest ABTS (2,2'-azino-bis[3-ethylbenzothiazoline-6-sulphonic acid]) scavenging activity; and thyme honey (Saouira) had the highest NO scavenging capacity. The antioxidant activity of Moroccan honeys was correlated with the phenol, flavonoid, and melanoidin contents. Dark honeys had higher antioxidant activity than light honeys. Samples with high sodium levels had lower free radical scavenging activity. On the other hand, calcium and magnesium increased the ABTS and peroxyl scavenging capacity, respectively, of honey samples. According to cluster and discriminant analyses, the honey samples were grouped in three clusters with respect to the phenol, flavonoid, melanoidin, proline, mineral and sugar contents, and free radical scavenging capacity.
Tribulus terrestris fruit has been used as a traditional and popular medicine for the prevention ... more Tribulus terrestris fruit has been used as a traditional and popular medicine for the prevention and treatment of several diseases, including sexual dysfunction, atherosclerosis and hypertension. The aim of this study was to evaluate the antioxidant, lipoxygenase and α-glucosidase inhibitory activities of an ethanol extract from Brazilian T. terrestris and its fractions sequentially partitioned into n-hexane, dichloromethane, ethyl acetate and n-butanol. The antioxidant capacities were determined by DPPH and ABTS scavenging free radicals, chelating metal ions, reducing power and total antioxidant activity by using phosphomolybdenum. High-performance liquid chromatography with diode array detection (HPLC-DAD) fingerprint analysis and quantitation of total phenolics were performed on the samples. The dichloromethane fraction showed the most complex HPLC-DAD chemical profile. The ethyl acetate and butanol fractions revealed the best phenolic compound and flavonoid recovery from T. terr...
International Journal of Food Science & Technology, 2021
SummaryThis study aims to determine the chemical composition of hemp (Cannabis sativa L.) seeds o... more SummaryThis study aims to determine the chemical composition of hemp (Cannabis sativa L.) seeds of two varieties cultivated in four different regions of northern Morocco. The content of protein, insoluble fibre, ash, total phenols and flavonoids was found to be 19.25%–24.18%, 26.40%–37.40%, 3.72%–5.39%, 134.57–199.90 mg 100g−1 and 39.40–69.54 mg 100g−1 respectively. Oil contents ranged from 26.42 to 35.19 g 100 g−1 of seeds in the four collection sites with variable oxidative stability at 100°C from 9.73 to 15.42 h. Total tocopherol content was between 376.46 and 796.06 mg kg−1 of oil, with a dominance of γ‐tocopherol. Fatty acids consisted mainly of unsaturated fatty acids (87.30‐88.96%) with a predominance of linoleic acid (48.26%–55.39%). Triacylglycerols (TAGs) composition analysis revealed the presence of 18 TAGs with an equivalent carbon number of 38–48. The composition of TAGs was predominated by LLL (14.34%–22.62%), OLL (12.77%–18.78%) and LLLn (12.32%–18.59%). Furthermore, ...
This study compared the capacity of propolis extract (PE) and thyme waste extract (TWE) to preven... more This study compared the capacity of propolis extract (PE) and thyme waste extract (TWE) to prevent the oxidation of oil in water (O/W) emulsion, as well as their impact on emulsion apparent viscosity (AV) in the presence of wheat germ and almond oils as lipid phase. For this, central composite design (CCD) and principal component analysis (PCA) were performed. Oxidation process was monitored by evaluating the formation of primary and secondary lipid oxidation products, at the same time the AV behavior was determined evaluating consistency index and flow behavior index. The results revealed that the increase of PE% and TWE% decreases TBARS (Thiobarbituric Acid Reactive Substances) and hydroperoxides formation. Viscosity increases with the rise of TWE% over (0.04%), whereas lower concentrations of PE% decreases it. Those results have been confirmed in the PCA analysis. TWE showed higher resistance to oxidation, although PE was more effective as antioxidant than TWE.
Journal of Essential Oil Bearing Plants, Nov 2, 2019
Abstract To evaluate the antioxidant activity of fourteen essential oils (EOs) isolated from myrt... more Abstract To evaluate the antioxidant activity of fourteen essential oils (EOs) isolated from myrtle (Myrtus communis L.) collected at different regions of Algeria, seven different assays were carried out: ability for scavenging ABTS (2,2-azinobis(3-ethylbenzothiazoline)-6-sulfonic acid), DPPH (2,2-diphenyl-1-picrylhydrazyl), HO (hydroxyl), NO (nitric oxide) free radicals; preventing lipid peroxidation through the thiobarbituric reactive species method, for reducing ferric ions to ferrous ions and chelating activity. The antiproliferative capacity of the EOs on Caco-2 cells were also evaluated using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method. The results showed that the essential oils were mainly constituted by α-pinene, 1,8-cineole, limonene and linalool. The EOs at different concentrations showed moderate to poor antioxidant activity. The sample Mc_Lf_06 was the single one that presented higher antioxidant activity across the four assays (prevention of lipid peroxidation, ability for scavenging the ABTS, NO and hydroxyl radicals). The EOs Mc_Lf_06 and Mc_Lf_10 showed the best antiproliferative activity but only achieving 20 % inhibition after 96 h of incubation for all concentrations tested. Intriguing the sample Mc_Lf_07 that stimulated the proliferation of Caco-2 cells, particularly at lower concentrations (0.1 and 0.2 mg/mL).
International Journal of Food Sciences and Nutrition, Jan 20, 2014
In this study, 17 commercial honey samples from Morocco were analyzed. Four samples did not meet ... more In this study, 17 commercial honey samples from Morocco were analyzed. Four samples did not meet the international physicochemical standards due to high hydroxymethylfurfural content and low diastase activity. Phenol content varied from 163.82 mg gallic acid equivalent (GAE)/kg in citrus honey to 923.70 mg (GAE)/kg in thyme honey from Rachidia; flavonoid content ranged from 4.26 mg quercetin equivalent (QE)/kg in citrus honey to 139.62 mg QE/kg in black cumin honey. Black cumin honey had the highest peroxyl scavenging activity; oregano (from Zaraphyt) and thyme honeys (from Rachidia) had the highest ABTS (2,2'-azino-bis[3-ethylbenzothiazoline-6-sulphonic acid]) scavenging activity; and thyme honey (Saouira) had the highest NO scavenging capacity. The antioxidant activity of Moroccan honeys was correlated with the phenol, flavonoid, and melanoidin contents. Dark honeys had higher antioxidant activity than light honeys. Samples with high sodium levels had lower free radical scavenging activity. On the other hand, calcium and magnesium increased the ABTS and peroxyl scavenging capacity, respectively, of honey samples. According to cluster and discriminant analyses, the honey samples were grouped in three clusters with respect to the phenol, flavonoid, melanoidin, proline, mineral and sugar contents, and free radical scavenging capacity.
Tribulus terrestris fruit has been used as a traditional and popular medicine for the prevention ... more Tribulus terrestris fruit has been used as a traditional and popular medicine for the prevention and treatment of several diseases, including sexual dysfunction, atherosclerosis and hypertension. The aim of this study was to evaluate the antioxidant, lipoxygenase and α-glucosidase inhibitory activities of an ethanol extract from Brazilian T. terrestris and its fractions sequentially partitioned into n-hexane, dichloromethane, ethyl acetate and n-butanol. The antioxidant capacities were determined by DPPH and ABTS scavenging free radicals, chelating metal ions, reducing power and total antioxidant activity by using phosphomolybdenum. High-performance liquid chromatography with diode array detection (HPLC-DAD) fingerprint analysis and quantitation of total phenolics were performed on the samples. The dichloromethane fraction showed the most complex HPLC-DAD chemical profile. The ethyl acetate and butanol fractions revealed the best phenolic compound and flavonoid recovery from T. terr...
International Journal of Food Science & Technology, 2021
SummaryThis study aims to determine the chemical composition of hemp (Cannabis sativa L.) seeds o... more SummaryThis study aims to determine the chemical composition of hemp (Cannabis sativa L.) seeds of two varieties cultivated in four different regions of northern Morocco. The content of protein, insoluble fibre, ash, total phenols and flavonoids was found to be 19.25%–24.18%, 26.40%–37.40%, 3.72%–5.39%, 134.57–199.90 mg 100g−1 and 39.40–69.54 mg 100g−1 respectively. Oil contents ranged from 26.42 to 35.19 g 100 g−1 of seeds in the four collection sites with variable oxidative stability at 100°C from 9.73 to 15.42 h. Total tocopherol content was between 376.46 and 796.06 mg kg−1 of oil, with a dominance of γ‐tocopherol. Fatty acids consisted mainly of unsaturated fatty acids (87.30‐88.96%) with a predominance of linoleic acid (48.26%–55.39%). Triacylglycerols (TAGs) composition analysis revealed the presence of 18 TAGs with an equivalent carbon number of 38–48. The composition of TAGs was predominated by LLL (14.34%–22.62%), OLL (12.77%–18.78%) and LLLn (12.32%–18.59%). Furthermore, ...
This study compared the capacity of propolis extract (PE) and thyme waste extract (TWE) to preven... more This study compared the capacity of propolis extract (PE) and thyme waste extract (TWE) to prevent the oxidation of oil in water (O/W) emulsion, as well as their impact on emulsion apparent viscosity (AV) in the presence of wheat germ and almond oils as lipid phase. For this, central composite design (CCD) and principal component analysis (PCA) were performed. Oxidation process was monitored by evaluating the formation of primary and secondary lipid oxidation products, at the same time the AV behavior was determined evaluating consistency index and flow behavior index. The results revealed that the increase of PE% and TWE% decreases TBARS (Thiobarbituric Acid Reactive Substances) and hydroperoxides formation. Viscosity increases with the rise of TWE% over (0.04%), whereas lower concentrations of PE% decreases it. Those results have been confirmed in the PCA analysis. TWE showed higher resistance to oxidation, although PE was more effective as antioxidant than TWE.
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