The 18th World Lake Conference, entitled “Governance, Resilience, and Sustainability of Lakes for... more The 18th World Lake Conference, entitled “Governance, Resilience, and Sustainability of Lakes for a Better Society” was held virtually from November 9th to 11th, 2022 at the University of Guanajuato, Mexico. This forum was designed with the primary objective of providing a space where scientists, professionals, students, managers, and practitioners could propose, share, and learn about studies, strategies, and actions which enhance the management of lakes around the world. Different activities were contemplated and scheduled in the organization of this forum, such as conferences, special sessions, technical sessions, and workshops where 194 distinguished investigators and participants shared the results of their research and the knowledge they have gained over the years.
Chronic liver disease (CLD) constitutes a growing global health issue, with no effective treatmen... more Chronic liver disease (CLD) constitutes a growing global health issue, with no effective treatments currently available. Oxidative stress closely interacts with other cellular and molecular processes to trigger stress pathways in different hepatic cells and fuel the development of liver fibrosis. Therefore, inhibition of reactive oxygen species (ROS)-mediated effects and modulation of major antioxidant responses to counteract oxidative stress-induced damage have emerged as interesting targets to prevent or ameliorate liver injury. Although many preclinical studies have shown that dietary supplements with antioxidant properties can significantly prevent CLD progression in animal models, this strategy has not proved effective to significantly reduce fibrosis when translated into clinical trials. Novel and more specific therapeutic approaches are thus required to alleviate oxidative stress and reduce liver fibrosis. We have reviewed the relevant literature concerning the crucial role o...
Supplementary Figure 2 from Cells Silenced for SDHB Expression Display Characteristic Features of... more Supplementary Figure 2 from Cells Silenced for SDHB Expression Display Characteristic Features of the Tumor Phenotype
Supplementary Table 2 from Cells Silenced for SDHB Expression Display Characteristic Features of ... more Supplementary Table 2 from Cells Silenced for SDHB Expression Display Characteristic Features of the Tumor Phenotype
Supplementary Figure 1 from Cells Silenced for SDHB Expression Display Characteristic Features of... more Supplementary Figure 1 from Cells Silenced for SDHB Expression Display Characteristic Features of the Tumor Phenotype
Cellular response to limiting oxygen levels is managed, in part, by the transcription factor hypo... more Cellular response to limiting oxygen levels is managed, in part, by the transcription factor hypoxia-inducible factor 1 (HIF-1), and the prolyl hydroxylase (PHD) family of oxygen-requiring enzymes. In the process of analyzing the expression of PHD3, we observed the presence of two alternatively processed PHD3 transcripts, designated PHD3Delta1 and PHD3Delta4 . The expression of both PHD3 and PHD3Delta1 was observed in all tissues and cell lines tested, although the expression of the novel PHD3Delta4 appeared to be restricted to primary cancer tissues. The function of PHD3Delta4 was assessed in transfection experiments showing a preserved prolyl hydroxylase activity. We would submit that PHD3 variants generated by alternative splicing may be intrinsically involved in the complex system of oxygen sensing.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2012
The medical use of nitroglycerin (GTN) is limited by patient tolerance. The present study evaluat... more The medical use of nitroglycerin (GTN) is limited by patient tolerance. The present study evaluated the role of mitochondrial Complex I in GTN biotransformation and the therapeutic effect of mitochondrial antioxidants. The development of GTN tolerance (in rat and human vessels) produced a decrease in mitochondrial O(2) consumption. Co-incubation with the mitochondria-targeted antioxidant mitoquinone (MQ, 10(-6)mol/L) or with glutathione ester (GEE, 10(-4)mol/L) blocked GTN tolerance and the effects of GTN on mitochondrial respiration and aldehyde dehydrogenase 2 (ALDH-2) activity. Biotransformation of GTN depended on the mitochondria being functionally active, particularly mitochondrial Complex I. Tolerance induced mitochondrial ROS production and oxidative stress, though these effects were not detected in HUVECρ(0) cells or Complex I mutant cells. Experiments performed to evaluate Complex I-dependent respiration demonstrated that its inhibition by GTN was prevented by the antioxidants in control samples. These results point to a key role for mitochondrial Complex I in the adequate functioning of ALDH-2. In addition, we have identified mitochondrial Complex I as one of the targets at which the initial oxidative stress responsible for GTN tolerance takes place. Our data also suggest a role for mitochondrial-antioxidants as therapeutic tools in the control of the tolerance that accompanies chronic nitrate use.
The 18th World Lake Conference, entitled “Governance, Resilience, and Sustainability of Lakes for... more The 18th World Lake Conference, entitled “Governance, Resilience, and Sustainability of Lakes for a Better Society” was held virtually from November 9th to 11th, 2022 at the University of Guanajuato, Mexico. This forum was designed with the primary objective of providing a space where scientists, professionals, students, managers, and practitioners could propose, share, and learn about studies, strategies, and actions which enhance the management of lakes around the world. Different activities were contemplated and scheduled in the organization of this forum, such as conferences, special sessions, technical sessions, and workshops where 194 distinguished investigators and participants shared the results of their research and the knowledge they have gained over the years.
Chronic liver disease (CLD) constitutes a growing global health issue, with no effective treatmen... more Chronic liver disease (CLD) constitutes a growing global health issue, with no effective treatments currently available. Oxidative stress closely interacts with other cellular and molecular processes to trigger stress pathways in different hepatic cells and fuel the development of liver fibrosis. Therefore, inhibition of reactive oxygen species (ROS)-mediated effects and modulation of major antioxidant responses to counteract oxidative stress-induced damage have emerged as interesting targets to prevent or ameliorate liver injury. Although many preclinical studies have shown that dietary supplements with antioxidant properties can significantly prevent CLD progression in animal models, this strategy has not proved effective to significantly reduce fibrosis when translated into clinical trials. Novel and more specific therapeutic approaches are thus required to alleviate oxidative stress and reduce liver fibrosis. We have reviewed the relevant literature concerning the crucial role o...
Supplementary Figure 2 from Cells Silenced for SDHB Expression Display Characteristic Features of... more Supplementary Figure 2 from Cells Silenced for SDHB Expression Display Characteristic Features of the Tumor Phenotype
Supplementary Table 2 from Cells Silenced for SDHB Expression Display Characteristic Features of ... more Supplementary Table 2 from Cells Silenced for SDHB Expression Display Characteristic Features of the Tumor Phenotype
Supplementary Figure 1 from Cells Silenced for SDHB Expression Display Characteristic Features of... more Supplementary Figure 1 from Cells Silenced for SDHB Expression Display Characteristic Features of the Tumor Phenotype
Cellular response to limiting oxygen levels is managed, in part, by the transcription factor hypo... more Cellular response to limiting oxygen levels is managed, in part, by the transcription factor hypoxia-inducible factor 1 (HIF-1), and the prolyl hydroxylase (PHD) family of oxygen-requiring enzymes. In the process of analyzing the expression of PHD3, we observed the presence of two alternatively processed PHD3 transcripts, designated PHD3Delta1 and PHD3Delta4 . The expression of both PHD3 and PHD3Delta1 was observed in all tissues and cell lines tested, although the expression of the novel PHD3Delta4 appeared to be restricted to primary cancer tissues. The function of PHD3Delta4 was assessed in transfection experiments showing a preserved prolyl hydroxylase activity. We would submit that PHD3 variants generated by alternative splicing may be intrinsically involved in the complex system of oxygen sensing.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2012
The medical use of nitroglycerin (GTN) is limited by patient tolerance. The present study evaluat... more The medical use of nitroglycerin (GTN) is limited by patient tolerance. The present study evaluated the role of mitochondrial Complex I in GTN biotransformation and the therapeutic effect of mitochondrial antioxidants. The development of GTN tolerance (in rat and human vessels) produced a decrease in mitochondrial O(2) consumption. Co-incubation with the mitochondria-targeted antioxidant mitoquinone (MQ, 10(-6)mol/L) or with glutathione ester (GEE, 10(-4)mol/L) blocked GTN tolerance and the effects of GTN on mitochondrial respiration and aldehyde dehydrogenase 2 (ALDH-2) activity. Biotransformation of GTN depended on the mitochondria being functionally active, particularly mitochondrial Complex I. Tolerance induced mitochondrial ROS production and oxidative stress, though these effects were not detected in HUVECρ(0) cells or Complex I mutant cells. Experiments performed to evaluate Complex I-dependent respiration demonstrated that its inhibition by GTN was prevented by the antioxidants in control samples. These results point to a key role for mitochondrial Complex I in the adequate functioning of ALDH-2. In addition, we have identified mitochondrial Complex I as one of the targets at which the initial oxidative stress responsible for GTN tolerance takes place. Our data also suggest a role for mitochondrial-antioxidants as therapeutic tools in the control of the tolerance that accompanies chronic nitrate use.
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Papers by Nadezda Apostolova