ABSTRACT We present hyper spectral imaging in the high wavenumber region of thick tissue samples ... more ABSTRACT We present hyper spectral imaging in the high wavenumber region of thick tissue samples made possible by a wavelength-swept CARS spectroscopy system where the Raman lines are excited sequentially at rates of up to 50,000 wavenumber per seconds.
We present a wavelength-swept coherent anti-Stokes Raman scattering (WS-CARS) spectroscopy system... more We present a wavelength-swept coherent anti-Stokes Raman scattering (WS-CARS) spectroscopy system for hyperspectral imaging in thick tissue. We use a strategy where the Raman lines are excited sequentially, circumventing the need for a spectrometer. This fibre laser system, consisting of a pump laser synchronized with a rapidly tunable programmable laser (PL), can access Raman lines over a significant fraction of the high wavenumber region (2700–2950 cm−1) at rates of up to 10,000 spectral points per second. To demonstrate its capabilities, we have acquired WS-CARS spectra of several samples as well as images and hyperspectral images (HSI) of thick tissue both in forward and epi-detection. This instrument should be especially useful in providing local biochemical information with surrounding context supplied by imaging.
ABSTRACT We present hyper spectral imaging in the high wavenumber region of thick tissue samples ... more ABSTRACT We present hyper spectral imaging in the high wavenumber region of thick tissue samples made possible by a wavelength-swept CARS spectroscopy system where the Raman lines are excited sequentially at rates of up to 50,000 wavenumber per seconds.
We present a wavelength-swept coherent anti-Stokes Raman scattering (WS-CARS) spectroscopy system... more We present a wavelength-swept coherent anti-Stokes Raman scattering (WS-CARS) spectroscopy system for hyperspectral imaging in thick tissue. We use a strategy where the Raman lines are excited sequentially, circumventing the need for a spectrometer. This fibre laser system, consisting of a pump laser synchronized with a rapidly tunable programmable laser (PL), can access Raman lines over a significant fraction of the high wavenumber region (2700–2950 cm−1) at rates of up to 10,000 spectral points per second. To demonstrate its capabilities, we have acquired WS-CARS spectra of several samples as well as images and hyperspectral images (HSI) of thick tissue both in forward and epi-detection. This instrument should be especially useful in providing local biochemical information with surrounding context supplied by imaging.
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Papers by Steve Begin