Ulkemizin sosyo-ekonomik acidan en onemli sektorlerinden biri olan gida sektoru son yillarda hizl... more Ulkemizin sosyo-ekonomik acidan en onemli sektorlerinden biri olan gida sektoru son yillarda hizla gelisim gostermektedir. Bursa, ozellikle meyve, sebze ve mamulleri sanayi, sut ve urunleri sanayi ve icecek sektoru acisindan ulkemiz de marka sehirlerden biri haline gelmistir. Ozellikle ihracat rakamlari incelendiginde Bursa gida sanayinin, ulke ihracat rakamlari arasinda onemli bir yere sahip oldugu gorulmektedir. Bu calisma, Bursa’da faaliyet gosteren gida firmalara yonelik sektorel analizin yapilmasi, Arastirma-Gelistirme (Ar-Ge) ve test-analiz ihtiyaclarinin belirlenmesi amaciyla yapilmistir. Buna ek olarak bu alanlardaki sorunlarinin belirlenmesi ve cozum onerilerinin gelistirilmesi hedeflenmistir. Sonuc olarak, Bursa’da faaliyet gosteren gida sanayi firmalarinin laboratuvar test ve analiz ihtiyaclari ve Ar-Ge calismalarinda gorev alacak nitelikli personel ile ilgili eksiklikleri oldugu belirlenmistir. Bu sorunlarin giderilmesinde universitelere onemli bir rol dustugu ve univers...
Journal of Agricultural Science and Technology, 2013
Using HPLC, the seasonal changes in the phenolic compound content of the leaves of the Gemlik oli... more Using HPLC, the seasonal changes in the phenolic compound content of the leaves of the Gemlik olive cultivar ( Olea europaea L) has been investigated with respect to the effects on the alternate bearing of the cultivar. For this purpose, the leaf concentrations of oleuropein, chlorogenic acid, caffeic acid, 3-hydroxycinnamic acid, scopolin and pcoumaric acid were analyzed at 10 day intervals around the years. The quantity and distribution of these phenolics in the leaves showed significant differences in 2008 (off year) and 2009 (on year). In the “on” year, the levels of chlorogenic and p-coumaric acids were high, whereas the abundance of other phenolic compounds was low. In contrast, during the “off” year, the chlorogenic and p-coumaric acid levels were at low levels, whereas the levels of the other phenolics were high. We found a negative relationship between the chlorogenic acid and caffeic acid concentrations in the “on” and “off” years: the amount of caffeic acid in the leaves ...
The differentiation of olive floral buds during winter is strictly correlated with flowering in t... more The differentiation of olive floral buds during winter is strictly correlated with flowering in the spring and ultimately with fruit production in autumn and the determination of the time of flower bud induction is important for determining the possible causes of alternate bearing and for improving management practices to correct alternate bearing. The aim of this research was to study the time of flower bud differentiation and developmental steps in the ‘Gemlik’ olive cultivar in 2008 (off year) and 2009 (on year). The sequence of initiation of the floral organs in each flower bud was sepals, stamens, petals, and gynoecium. There was no visible difference between the time of differentiation and the developmental stage of the floral organs with respect to the ‘on’ and ‘off’ years during the study.
Maize transposable elements, Activator (Ac) and Ds transformed into several heterologous plant sp... more Maize transposable elements, Activator (Ac) and Ds transformed into several heterologous plant species for transposon tagging of genes. Several genes in Arabidopsis, flax, petunia, tobacco, and tomato have been tagged and cloned by using Ac and Ds. We have double transformed carrot lines, B493 and B7262 with stabilized autonomous Ac and non-autonomous Ds element to develop a two-element based transposon tagging system. PCR and Southern hybridization indicated that Ds element transposed from T-DNA in calli, somatic embryos and transgenic plants. The insertion of Ds element into new sites in carrot genome after excision verified by GUS assay, Southern hybridization and inverse-PCR. Currently, the behavior of non-autonomous Ds element is being studied. Ds induced mutation will be screened in transgenic plants. These initial results demonstrate that the Ac/Ds-based transposon tagging system may work in carrot.
Journal of the American Society for Horticultural Science, 2007
Production of a visible flower stalk, or bolting, has been used as a major trait to categorize ga... more Production of a visible flower stalk, or bolting, has been used as a major trait to categorize garlic (Allium sativum L.) clones. Analysis of mitochondrial genome variation with polymerase chain reaction (PCR) revealed differences between bolting and nonbolting clones of garlic. Screening 333 garlic accessions from diverse geographic origins revealed a 1403-bp mitochondrial DNA marker associated with bolting that the authors call “Bolt Marker” (BltM). Bolt Marker did not amplify in any of the 131 nonbolting clones, whereas amplification of this marker was observed in 127 of 130 (97.7%) garlic clones that bolted completely in Wisconsin. Seventy-two garlic clones bolted incompletely (clones in which some but not all of the plants bolted), and this marker was not amplified in 69 (95.8%) of these clones. Because of the significant association of BltM with bolting, this PCR-based marker can be used to discriminate complete-bolting garlic clones reliably from nonbolting and incomplete-bol...
Journal of the American Society for Horticultural Science, 2003
Garlic (Allium sativum L.) is an asexually propagated crop that displays much morphological diver... more Garlic (Allium sativum L.) is an asexually propagated crop that displays much morphological diversity. Studies which have assessed garlic diversity with isozymes and randomly amplified polymorphic DNA (RAPD) markers generally agreed with the morphological observations but sometimes failed to discriminate clones. To discriminate among closely related garlic clones in more detail, we introduced amplified fragment-length polymorphism (AFLPs) to evaluate the genetic diversity and phenetic relatedness of 45 garlic clones and three A. longicuspis clones and we compared AFLP results with RAPD markers and isozymes. Three AFLP primer combinations generated a total of 183 polymorphic fragments. Although similarities between the clusters were low (≥0.30), some clones within the clusters were very similar (>0.95) with AFLP analysis. Sixteen clones represented only six different banding patterns, within which they shared 100% polymorphic AFLPs and RAPD markers, and likely are duplicates. In a...
Fig (Ficus carica L.) is a traditional crop in Turkey and widely cultivated around the Mediterran... more Fig (Ficus carica L.) is a traditional crop in Turkey and widely cultivated around the Mediterranean areas. The gynodioecious fig species is present in two sexual forms, i.e. the domesticated fig (female tree) and the caprifig (male tree). Caprifigs are crucial for high quality fig production and breeding while, the studies on assessment of genetic relationship among caprifigs is limited. The aim of this study was to determine genetic diversity among 45 caprifigs and 2 female figs collected from four provinces in Marmara and Aegean Sea Regions of Turkey using simple sequence repeat (SSR) markers. In this work, 24 SSR markers were tested, one was monomorphic and the remaining markers amplified 82 alleles. The number of polymorphic alleles per SSR marker ranged from 2 to 7. The observed heterozygosity (Ho) differed from 0.18 to 0.76 and expected heterozygosity (He) ranged between 0.24 and 0.81. The polymorphism information content (PIC) varied from 0.42 to 0.98. A UPGMA analysis based...
High quality olive oil can be produced from a standard cultivar with high olive oil quality. In t... more High quality olive oil can be produced from a standard cultivar with high olive oil quality. In this study, olive genotypes grown in the Southern Marmara Region has been determined using simple sequence repeats (SSR) markers. A total of 70 samples from 10 major olive growing ...
Genetic linkage maps are valuable tools for genetic, genomic, and crop breeding studies. Several ... more Genetic linkage maps are valuable tools for genetic, genomic, and crop breeding studies. Several genetic linkage maps were constructed for the olive (Olea europaea L.) genome, mainly using amplified fragment length polymorphisms (AFLPs) and simple sequence repeat (SSR) markers. However, AFLPs and SSR markers were not enough to develop a high-density olive linkage map. Genotyping-by-sequencing (GBS), a recently developed single-nucleotide polymorphism (SNP) identification methodology based on next-generation sequencing (NGS) technologies, has been demonstrated to be useful for the identification of a high number of SNP markers and the construction of high-density genetic linkage maps. In the present study, we identified a total of 10,941 SNPs from a cross between the olive cultivars 'Gemlik' and 'Edincik Su' using GBS and de novo SNP discovery implemented in the computer program "Stacks." A high-density genetic linkage map for the olive genome was constructe...
Ulkemizin sosyo-ekonomik acidan en onemli sektorlerinden biri olan gida sektoru son yillarda hizl... more Ulkemizin sosyo-ekonomik acidan en onemli sektorlerinden biri olan gida sektoru son yillarda hizla gelisim gostermektedir. Bursa, ozellikle meyve, sebze ve mamulleri sanayi, sut ve urunleri sanayi ve icecek sektoru acisindan ulkemiz de marka sehirlerden biri haline gelmistir. Ozellikle ihracat rakamlari incelendiginde Bursa gida sanayinin, ulke ihracat rakamlari arasinda onemli bir yere sahip oldugu gorulmektedir. Bu calisma, Bursa’da faaliyet gosteren gida firmalara yonelik sektorel analizin yapilmasi, Arastirma-Gelistirme (Ar-Ge) ve test-analiz ihtiyaclarinin belirlenmesi amaciyla yapilmistir. Buna ek olarak bu alanlardaki sorunlarinin belirlenmesi ve cozum onerilerinin gelistirilmesi hedeflenmistir. Sonuc olarak, Bursa’da faaliyet gosteren gida sanayi firmalarinin laboratuvar test ve analiz ihtiyaclari ve Ar-Ge calismalarinda gorev alacak nitelikli personel ile ilgili eksiklikleri oldugu belirlenmistir. Bu sorunlarin giderilmesinde universitelere onemli bir rol dustugu ve univers...
Journal of Agricultural Science and Technology, 2013
Using HPLC, the seasonal changes in the phenolic compound content of the leaves of the Gemlik oli... more Using HPLC, the seasonal changes in the phenolic compound content of the leaves of the Gemlik olive cultivar ( Olea europaea L) has been investigated with respect to the effects on the alternate bearing of the cultivar. For this purpose, the leaf concentrations of oleuropein, chlorogenic acid, caffeic acid, 3-hydroxycinnamic acid, scopolin and pcoumaric acid were analyzed at 10 day intervals around the years. The quantity and distribution of these phenolics in the leaves showed significant differences in 2008 (off year) and 2009 (on year). In the “on” year, the levels of chlorogenic and p-coumaric acids were high, whereas the abundance of other phenolic compounds was low. In contrast, during the “off” year, the chlorogenic and p-coumaric acid levels were at low levels, whereas the levels of the other phenolics were high. We found a negative relationship between the chlorogenic acid and caffeic acid concentrations in the “on” and “off” years: the amount of caffeic acid in the leaves ...
The differentiation of olive floral buds during winter is strictly correlated with flowering in t... more The differentiation of olive floral buds during winter is strictly correlated with flowering in the spring and ultimately with fruit production in autumn and the determination of the time of flower bud induction is important for determining the possible causes of alternate bearing and for improving management practices to correct alternate bearing. The aim of this research was to study the time of flower bud differentiation and developmental steps in the ‘Gemlik’ olive cultivar in 2008 (off year) and 2009 (on year). The sequence of initiation of the floral organs in each flower bud was sepals, stamens, petals, and gynoecium. There was no visible difference between the time of differentiation and the developmental stage of the floral organs with respect to the ‘on’ and ‘off’ years during the study.
Maize transposable elements, Activator (Ac) and Ds transformed into several heterologous plant sp... more Maize transposable elements, Activator (Ac) and Ds transformed into several heterologous plant species for transposon tagging of genes. Several genes in Arabidopsis, flax, petunia, tobacco, and tomato have been tagged and cloned by using Ac and Ds. We have double transformed carrot lines, B493 and B7262 with stabilized autonomous Ac and non-autonomous Ds element to develop a two-element based transposon tagging system. PCR and Southern hybridization indicated that Ds element transposed from T-DNA in calli, somatic embryos and transgenic plants. The insertion of Ds element into new sites in carrot genome after excision verified by GUS assay, Southern hybridization and inverse-PCR. Currently, the behavior of non-autonomous Ds element is being studied. Ds induced mutation will be screened in transgenic plants. These initial results demonstrate that the Ac/Ds-based transposon tagging system may work in carrot.
Journal of the American Society for Horticultural Science, 2007
Production of a visible flower stalk, or bolting, has been used as a major trait to categorize ga... more Production of a visible flower stalk, or bolting, has been used as a major trait to categorize garlic (Allium sativum L.) clones. Analysis of mitochondrial genome variation with polymerase chain reaction (PCR) revealed differences between bolting and nonbolting clones of garlic. Screening 333 garlic accessions from diverse geographic origins revealed a 1403-bp mitochondrial DNA marker associated with bolting that the authors call “Bolt Marker” (BltM). Bolt Marker did not amplify in any of the 131 nonbolting clones, whereas amplification of this marker was observed in 127 of 130 (97.7%) garlic clones that bolted completely in Wisconsin. Seventy-two garlic clones bolted incompletely (clones in which some but not all of the plants bolted), and this marker was not amplified in 69 (95.8%) of these clones. Because of the significant association of BltM with bolting, this PCR-based marker can be used to discriminate complete-bolting garlic clones reliably from nonbolting and incomplete-bol...
Journal of the American Society for Horticultural Science, 2003
Garlic (Allium sativum L.) is an asexually propagated crop that displays much morphological diver... more Garlic (Allium sativum L.) is an asexually propagated crop that displays much morphological diversity. Studies which have assessed garlic diversity with isozymes and randomly amplified polymorphic DNA (RAPD) markers generally agreed with the morphological observations but sometimes failed to discriminate clones. To discriminate among closely related garlic clones in more detail, we introduced amplified fragment-length polymorphism (AFLPs) to evaluate the genetic diversity and phenetic relatedness of 45 garlic clones and three A. longicuspis clones and we compared AFLP results with RAPD markers and isozymes. Three AFLP primer combinations generated a total of 183 polymorphic fragments. Although similarities between the clusters were low (≥0.30), some clones within the clusters were very similar (>0.95) with AFLP analysis. Sixteen clones represented only six different banding patterns, within which they shared 100% polymorphic AFLPs and RAPD markers, and likely are duplicates. In a...
Fig (Ficus carica L.) is a traditional crop in Turkey and widely cultivated around the Mediterran... more Fig (Ficus carica L.) is a traditional crop in Turkey and widely cultivated around the Mediterranean areas. The gynodioecious fig species is present in two sexual forms, i.e. the domesticated fig (female tree) and the caprifig (male tree). Caprifigs are crucial for high quality fig production and breeding while, the studies on assessment of genetic relationship among caprifigs is limited. The aim of this study was to determine genetic diversity among 45 caprifigs and 2 female figs collected from four provinces in Marmara and Aegean Sea Regions of Turkey using simple sequence repeat (SSR) markers. In this work, 24 SSR markers were tested, one was monomorphic and the remaining markers amplified 82 alleles. The number of polymorphic alleles per SSR marker ranged from 2 to 7. The observed heterozygosity (Ho) differed from 0.18 to 0.76 and expected heterozygosity (He) ranged between 0.24 and 0.81. The polymorphism information content (PIC) varied from 0.42 to 0.98. A UPGMA analysis based...
High quality olive oil can be produced from a standard cultivar with high olive oil quality. In t... more High quality olive oil can be produced from a standard cultivar with high olive oil quality. In this study, olive genotypes grown in the Southern Marmara Region has been determined using simple sequence repeats (SSR) markers. A total of 70 samples from 10 major olive growing ...
Genetic linkage maps are valuable tools for genetic, genomic, and crop breeding studies. Several ... more Genetic linkage maps are valuable tools for genetic, genomic, and crop breeding studies. Several genetic linkage maps were constructed for the olive (Olea europaea L.) genome, mainly using amplified fragment length polymorphisms (AFLPs) and simple sequence repeat (SSR) markers. However, AFLPs and SSR markers were not enough to develop a high-density olive linkage map. Genotyping-by-sequencing (GBS), a recently developed single-nucleotide polymorphism (SNP) identification methodology based on next-generation sequencing (NGS) technologies, has been demonstrated to be useful for the identification of a high number of SNP markers and the construction of high-density genetic linkage maps. In the present study, we identified a total of 10,941 SNPs from a cross between the olive cultivars 'Gemlik' and 'Edincik Su' using GBS and de novo SNP discovery implemented in the computer program "Stacks." A high-density genetic linkage map for the olive genome was constructe...
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