The Southeast Asian journal of tropical medicine and public health, 2014
Vibrio vulnificus can cause septicemia, wound infection and gastroenteritis. The most severe infe... more Vibrio vulnificus can cause septicemia, wound infection and gastroenteritis. The most severe infections are related to consumption of raw or undercooked seafood. Virulence genes, biomarkers, antimicrobial resistance, and genetic relationships among V vulnificus isolated from clinical and environmental sources in Thailand have not hitherto been investigated. ViuB encoding vulnibactin siderophore was detected in 33% and 50% of clinical and environmental (cockle) V. vulnificus isolates, respectively, and capsular polysaccharide allele 1 in 67% and 75% of clinical and environmental isolates, respectively. Analysis of the 16 S rDNA gene revealed that type B was the most frequent in both clinical and environmental isolates (67%) whereas the non type-able (30%) was detected only in environmental isolates. The virulence-correlated gene (vcg) with both type C and E together was the most frequently found among the clinical (67%) and environmental (72%) isolates. Pulsed-field gel electrophores...
Applied and environmental microbiology, Jan 2, 2015
V. cholerae non-O1/non-O139 inhabits estuarine and coastal waters globally but its clinical signi... more V. cholerae non-O1/non-O139 inhabits estuarine and coastal waters globally but its clinical significance has not been sufficiently investigated, despite the fact that it has been associated with septicemia and gastroenteritis. Emergence of virulent V. cholerae non-O1/non-O139 is consistent with recognition of new pathogenic variants worldwide. Oyster, sediment, and water samples were collected during a Vibrio surveillance program carried out in 2009-2012 in the Chesapeake Bay, Maryland (USA). V. cholerae O1 was detected by direct fluorescent antibody (DFA) but not successfully cultured, whereas 395 isolates of V. cholerae non-O1/non-O139 were confirmed by multiplex PCR and serology. Only a few of the V. cholerae non-O1/non-O139 isolates were resistant to ampicillin and/or penicillin. Most were sensitive to all antibiotics tested and 77-90% carried hemolysin hlyAET, actin cross-linking repeats in toxin rtxA, haemagglutinin protease hap, and type 6 secretion system. Ca. 19 to 21% of t...
The Southeast Asian journal of tropical medicine and public health, 2014
Vibrio vulnificus can cause septicemia, wound infection and gastroenteritis. The most severe infe... more Vibrio vulnificus can cause septicemia, wound infection and gastroenteritis. The most severe infections are related to consumption of raw or undercooked seafood. Virulence genes, biomarkers, antimicrobial resistance, and genetic relationships among V vulnificus isolated from clinical and environmental sources in Thailand have not hitherto been investigated. ViuB encoding vulnibactin siderophore was detected in 33% and 50% of clinical and environmental (cockle) V. vulnificus isolates, respectively, and capsular polysaccharide allele 1 in 67% and 75% of clinical and environmental isolates, respectively. Analysis of the 16 S rDNA gene revealed that type B was the most frequent in both clinical and environmental isolates (67%) whereas the non type-able (30%) was detected only in environmental isolates. The virulence-correlated gene (vcg) with both type C and E together was the most frequently found among the clinical (67%) and environmental (72%) isolates. Pulsed-field gel electrophores...
Applied and environmental microbiology, Jan 2, 2015
V. cholerae non-O1/non-O139 inhabits estuarine and coastal waters globally but its clinical signi... more V. cholerae non-O1/non-O139 inhabits estuarine and coastal waters globally but its clinical significance has not been sufficiently investigated, despite the fact that it has been associated with septicemia and gastroenteritis. Emergence of virulent V. cholerae non-O1/non-O139 is consistent with recognition of new pathogenic variants worldwide. Oyster, sediment, and water samples were collected during a Vibrio surveillance program carried out in 2009-2012 in the Chesapeake Bay, Maryland (USA). V. cholerae O1 was detected by direct fluorescent antibody (DFA) but not successfully cultured, whereas 395 isolates of V. cholerae non-O1/non-O139 were confirmed by multiplex PCR and serology. Only a few of the V. cholerae non-O1/non-O139 isolates were resistant to ampicillin and/or penicillin. Most were sensitive to all antibiotics tested and 77-90% carried hemolysin hlyAET, actin cross-linking repeats in toxin rtxA, haemagglutinin protease hap, and type 6 secretion system. Ca. 19 to 21% of t...
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Papers by Shah M Rashed