Malocclusion is a condition characterized by diverse phenotypic expression patterns, with a compl... more Malocclusion is a condition characterized by diverse phenotypic expression patterns, with a complex underlying genetic background. COL1a1 is one of the genes that has been previously associated with malocclusion, with one particular SNP, rs2249492 (C>G, C>T), having been linked with an increased risk of skeletal class II malocclusion. In this paper, making use of DNA sequencing and cephalometric measurements, we present preliminary data regarding the association between the rs2249492 SNP and the risk of malocclusion in the Romanian population, illustrated as continuous, rather than categorical phenotypes. The results show a tendency towards a Class II pattern determined by mandibular retrognathism, rather than maxillary prognathism among the individuals possessing the mutant allele. Subsequent studies on larger sample sizes should include statistical analysis focused on associations between the rs2249492 allele and continuous phenotypic variation inside, but not restricted to Class II malocclusion, in order to acquire a more detailed picture of the interaction between the polymorphism and this complex condition
Introduction: Myofunctional therapy is becoming an increasingly recommended treatment option. The... more Introduction: Myofunctional therapy is becoming an increasingly recommended treatment option. The objective of this case presentation was to highlight the advantages and disadvantages of this therapeutic approach. Discussions: The objectives of myofunctional treatment are the morphological, functional, and aesthetic balancing of the skeletal, muscular, and dental systems, and the elimination of dysfunctional factors. This therapeutic approach is addressed to patients in the growth period, uses relatively simple means but requires very good compliance from the patient and the family. The patient's motivation and will are individual factors that play an important role in the success of such treatment. Conclusions: Myofunctional therapy requires an individualized approach, according to the psychological characteristics of the child, to achieve the desired results and not just follow the treatment objectives derived from the diagnosis of the dento-maxillary anomaly. Rezumat Introduc...
Bulletin of the Transilvania University of Brasov. Series VI:Medical Sciences, 2022
Given multiple causalities, the fetus develops an intrauterine growth restriction that compensato... more Given multiple causalities, the fetus develops an intrauterine growth restriction that compensatory stimulates the adaptative phenomena; both, the determining cause of intrauterine growth restriction and the adaptative reactions, will initiate epigenetic changes which will affect the long-term structure and functionality of the heart and will modify the cardiovascular reactivity. The study is based on a systematic review of 20 existing studies that certifies a link between epigenetic changes and adult cardiovascular disease.Against the background of epigenetic changes, postnatal nutritional and behavioral factors are added which lead to pathologies in adult life, so the proper functioning and development of the body starts from the first moments of intrauterine life and contuse postnatal, creating the premises for a healthy life. However, it is difficult to assess the environmental factors that interact with the human body and how this combination of factors determines a predisposit...
Disc displacement without reduction (DDwoR) with or without limited mouth opening is intracapsula... more Disc displacement without reduction (DDwoR) with or without limited mouth opening is intracapsular biomechanical disorder characterized by anterior position of the disc relative to condyle, that does not reduce with mouth opening. Due to a multifactorial etiology, the treatment of DDwoR needs an interdisciplinary team to address the specific factor. The ttreatment modalities may include :patient education and self-management,cognitive behavioral therapy(CBT),pharmacotherapy,physical therapy,orthopedic appliances,as conservative options and occlusal therapy and surgery, as aggressive,irreversible options.The aim of this article is to present a successfully treated case of DDwoR through conservative therapy.
Introduction: Assessment of maxillary transverse discrepancy requires an accurate tool in order t... more Introduction: Assessment of maxillary transverse discrepancy requires an accurate tool in order to implement the appropriate treatment plan. Aim: To evaluate the accuracy of Pont’s Index in confirming a transverse maxillary deficiency by comparing its results with the corresponding results yielded by the University of Pennsylvania CBCT analysis. Material and methods: The study was performed on dental casts and CBCT’s of 60 randomly selected patients by adopting a cluster sampling method. The results of Pont’s Index and University of Pennsylvania CBCT analysis were analyzed through McNemar’s test using Excel Data Analysis, and the accuracy indicators for Pont’s Index were computed using MedCalc Ltd. Results: Mc Nemar’s test revealed a p-value of 0.85. The accuracy indicators of Pont’s Index were: sensitivity: 69%; specificity: 16.6%; positive predictive value: 65%; negative predictive value: 18.75%; positive likelihood ratio: 0.83; negative likelihood ratio: 1.86; and accuracy: 53.28...
Background: The continue efforts for long term reducing of intraocular pressure (IOP) in patients... more Background: The continue efforts for long term reducing of intraocular pressure (IOP) in patients with open angle glaucoma, focused the attention on those patients who received different therapies, other that topical drugs for IOP reduction, that can influence this parameter.. The aim of this study was to evaluate the IOP, total oxidative stress and anti-oxidant capacity of plasma before and after nicergoline therapy in patients with ischemic stroke and associated open angle glaucoma. Material and method: a group of 35 patients with ischemic stroke and chronic open angle glaucoma (under topical beta-blockers therapy) was studied regarding the values of IOP, total oxidative stress (TOS) and total antioxidant capacity (TAC) before (T1) and after 6 month (T2) of nicergoline therapy. Results: IOP values for both eyes were significantly reduced when the values of T1 examination were compared with those of T2 assessment. The total oxidative stress parameter was also significantly reduced ...
The aim of this in vitro study was to assess the effect of toothpastes, with different compositio... more The aim of this in vitro study was to assess the effect of toothpastes, with different compositions, on optical and morphological features of sound and demineralized enamel. We selected twenty-five teeth, recently extracted for orthodontic purposes, for this in vitro study. The teeth were caries free, without stains, fissures, filling or hypoplasia observed at inspection under standard conditions. Teeth were brushed (for 2–3 min, twice a day, for 21 days), with five different toothpastes (four commercially available and an experimental one) containing fluoride and hydroxyapatite. After that, teeth were demineralized with 37% orthophosforic acid (Ultra Etch®, Ultradent Products Inc., South Jordan, UT, USA) for 60 s. We repeated the brushing protocol for another 21 days on demineralized enamel. Enamel vestibular surfaces were examined using a spectrophotometer (Vita EasyShade -Vita Zahnfabrik, Bad Sackingen, Germany) and a Scanning Electron Microscope (Inspect S®, FEI Company, Hillsbo...
Resin composite materials that are used to restore tooth cervical lesions associated with gingiva... more Resin composite materials that are used to restore tooth cervical lesions associated with gingival recessions can hamper healing after root coverage surgeries. This study evaluates thein vitrocytotoxic effect of five resin composites (two commercial and three experimental) on oral mesenchymal stem cells (MSCs) and the persistence of stemness properties in high passage MSCs. Sorption and solubility tests were made for all materials. MSCs were isolated from re-entry palatal and periodontal granulation tissues and were characterized and cultured on composite discs. Cytotoxicity of the materials was evaluated by the Alamar Blue viability test, by Paul Karl Horan (PKH) labeling, and by immunocytochemical staining for actin. Water and saliva sorption and solubility data revealed that two of the experimental materials behaved comparable with the marketed resin composites. The Alamar Blue viability test shows that both cell lines grew well on composite discs that seemed to induce no apparen...
Genetic polymorphisms in the vitamin D receptor gene (VDR) were related to bone mineral density, ... more Genetic polymorphisms in the vitamin D receptor gene (VDR) were related to bone mineral density, bone turnover, and diseases with bone loss, as in periodontitis. RUNX2 regulates transcription of numerous genes and thereby control osteoblast development from mesenchymal stem cells and maturation into osteocytes. During osteoblast development and maturation, RUNX2 cooperates also with other transcription factors and cofactors. Mutations in this gene have been associated with cleidocranial dysplasia, a bone development disorder. Mutations in the PAX9 gene were frequently associated with oligodontia or hypodontia, as well as with Class II/Division 2 malocclusions (with or without hypodontia). The aim of the present paper was to identify genes which we presume to be involved in the development of the craniofacial complex. We selected three markers: VDR (vitamin D receptor - rs731236), PAX9 (rs8004560) and RUNX2 (rs731236) in order to reveal their association with Class I, II and III dent...
Background. The Matrilin-1 gene is thought to be involved in the formation of the filamentous net... more Background. The Matrilin-1 gene is thought to be involved in the formation of the filamentous network of the cartilage’s extracellular matrix and implicitly in the formation of the mandibular condyle cartilage, very important in different malocclusions. Objectives. The purpose of this study was the association of the marker Myo1H (rs10850110) with malocclusions. Methods. The sample comprises 47 patients with different malocclusions. The control group included 11 individuals. Cephalometric measurements were performed. The genotyping was done by sequencing. Results. Significant differences concerning allele and genotype distribution were observed between different malocclusions and the control group. The genotypes were distributed in the population according to Hardy-Weinberg equilibrium. Conclusions. Our findings are in agreement with other studies that emphasize the importance of the Myo1H gene in the determination of Class I, II and especially Class III phenotypes.
Third molar presence in the infratemporal fossa is a rare event and it has been reported previous... more Third molar presence in the infratemporal fossa is a rare event and it has been reported previously only two times in the literature, except for the cases which arise from complications occurring during the extraction of the impacted upper third molar. Due to the presence of important vessel bundles and nerves in this area, third molar removal requires a correct surgical management in order to avoid many possible serious side effects. We report an unusual case of upper third molar detected in the infratemporal fossa, which has been thoroughly investigated radiologically and removed through a safe surgical approach.
Revista medico-chirurgicală̆ a Societă̆ţ̜ii de Medici ş̧i Naturaliş̧ti din Iaş̧i
In the era of fixed appliances, some orthodontic practitioners seem to forget about functional th... more In the era of fixed appliances, some orthodontic practitioners seem to forget about functional therapy. Functional appliances are the only capable of orthopedically changes during the growth spurt. Activators of all types, classic or opened, are elected appliances in growing subjects with class II/1 anomalies. The appropriate case selection, along with patient compliance, lead to improved facial aesthetic and dental occlusion, at affordable prices. With this case presentation, we want to show our protocol in the management of these cases.
Malocclusion is a condition characterized by diverse phenotypic expression patterns, with a compl... more Malocclusion is a condition characterized by diverse phenotypic expression patterns, with a complex underlying genetic background. COL1a1 is one of the genes that has been previously associated with malocclusion, with one particular SNP, rs2249492 (C>G, C>T), having been linked with an increased risk of skeletal class II malocclusion. In this paper, making use of DNA sequencing and cephalometric measurements, we present preliminary data regarding the association between the rs2249492 SNP and the risk of malocclusion in the Romanian population, illustrated as continuous, rather than categorical phenotypes. The results show a tendency towards a Class II pattern determined by mandibular retrognathism, rather than maxillary prognathism among the individuals possessing the mutant allele. Subsequent studies on larger sample sizes should include statistical analysis focused on associations between the rs2249492 allele and continuous phenotypic variation inside, but not restricted to Class II malocclusion, in order to acquire a more detailed picture of the interaction between the polymorphism and this complex condition
Introduction: Myofunctional therapy is becoming an increasingly recommended treatment option. The... more Introduction: Myofunctional therapy is becoming an increasingly recommended treatment option. The objective of this case presentation was to highlight the advantages and disadvantages of this therapeutic approach. Discussions: The objectives of myofunctional treatment are the morphological, functional, and aesthetic balancing of the skeletal, muscular, and dental systems, and the elimination of dysfunctional factors. This therapeutic approach is addressed to patients in the growth period, uses relatively simple means but requires very good compliance from the patient and the family. The patient's motivation and will are individual factors that play an important role in the success of such treatment. Conclusions: Myofunctional therapy requires an individualized approach, according to the psychological characteristics of the child, to achieve the desired results and not just follow the treatment objectives derived from the diagnosis of the dento-maxillary anomaly. Rezumat Introduc...
Bulletin of the Transilvania University of Brasov. Series VI:Medical Sciences, 2022
Given multiple causalities, the fetus develops an intrauterine growth restriction that compensato... more Given multiple causalities, the fetus develops an intrauterine growth restriction that compensatory stimulates the adaptative phenomena; both, the determining cause of intrauterine growth restriction and the adaptative reactions, will initiate epigenetic changes which will affect the long-term structure and functionality of the heart and will modify the cardiovascular reactivity. The study is based on a systematic review of 20 existing studies that certifies a link between epigenetic changes and adult cardiovascular disease.Against the background of epigenetic changes, postnatal nutritional and behavioral factors are added which lead to pathologies in adult life, so the proper functioning and development of the body starts from the first moments of intrauterine life and contuse postnatal, creating the premises for a healthy life. However, it is difficult to assess the environmental factors that interact with the human body and how this combination of factors determines a predisposit...
Disc displacement without reduction (DDwoR) with or without limited mouth opening is intracapsula... more Disc displacement without reduction (DDwoR) with or without limited mouth opening is intracapsular biomechanical disorder characterized by anterior position of the disc relative to condyle, that does not reduce with mouth opening. Due to a multifactorial etiology, the treatment of DDwoR needs an interdisciplinary team to address the specific factor. The ttreatment modalities may include :patient education and self-management,cognitive behavioral therapy(CBT),pharmacotherapy,physical therapy,orthopedic appliances,as conservative options and occlusal therapy and surgery, as aggressive,irreversible options.The aim of this article is to present a successfully treated case of DDwoR through conservative therapy.
Introduction: Assessment of maxillary transverse discrepancy requires an accurate tool in order t... more Introduction: Assessment of maxillary transverse discrepancy requires an accurate tool in order to implement the appropriate treatment plan. Aim: To evaluate the accuracy of Pont’s Index in confirming a transverse maxillary deficiency by comparing its results with the corresponding results yielded by the University of Pennsylvania CBCT analysis. Material and methods: The study was performed on dental casts and CBCT’s of 60 randomly selected patients by adopting a cluster sampling method. The results of Pont’s Index and University of Pennsylvania CBCT analysis were analyzed through McNemar’s test using Excel Data Analysis, and the accuracy indicators for Pont’s Index were computed using MedCalc Ltd. Results: Mc Nemar’s test revealed a p-value of 0.85. The accuracy indicators of Pont’s Index were: sensitivity: 69%; specificity: 16.6%; positive predictive value: 65%; negative predictive value: 18.75%; positive likelihood ratio: 0.83; negative likelihood ratio: 1.86; and accuracy: 53.28...
Background: The continue efforts for long term reducing of intraocular pressure (IOP) in patients... more Background: The continue efforts for long term reducing of intraocular pressure (IOP) in patients with open angle glaucoma, focused the attention on those patients who received different therapies, other that topical drugs for IOP reduction, that can influence this parameter.. The aim of this study was to evaluate the IOP, total oxidative stress and anti-oxidant capacity of plasma before and after nicergoline therapy in patients with ischemic stroke and associated open angle glaucoma. Material and method: a group of 35 patients with ischemic stroke and chronic open angle glaucoma (under topical beta-blockers therapy) was studied regarding the values of IOP, total oxidative stress (TOS) and total antioxidant capacity (TAC) before (T1) and after 6 month (T2) of nicergoline therapy. Results: IOP values for both eyes were significantly reduced when the values of T1 examination were compared with those of T2 assessment. The total oxidative stress parameter was also significantly reduced ...
The aim of this in vitro study was to assess the effect of toothpastes, with different compositio... more The aim of this in vitro study was to assess the effect of toothpastes, with different compositions, on optical and morphological features of sound and demineralized enamel. We selected twenty-five teeth, recently extracted for orthodontic purposes, for this in vitro study. The teeth were caries free, without stains, fissures, filling or hypoplasia observed at inspection under standard conditions. Teeth were brushed (for 2–3 min, twice a day, for 21 days), with five different toothpastes (four commercially available and an experimental one) containing fluoride and hydroxyapatite. After that, teeth were demineralized with 37% orthophosforic acid (Ultra Etch®, Ultradent Products Inc., South Jordan, UT, USA) for 60 s. We repeated the brushing protocol for another 21 days on demineralized enamel. Enamel vestibular surfaces were examined using a spectrophotometer (Vita EasyShade -Vita Zahnfabrik, Bad Sackingen, Germany) and a Scanning Electron Microscope (Inspect S®, FEI Company, Hillsbo...
Resin composite materials that are used to restore tooth cervical lesions associated with gingiva... more Resin composite materials that are used to restore tooth cervical lesions associated with gingival recessions can hamper healing after root coverage surgeries. This study evaluates thein vitrocytotoxic effect of five resin composites (two commercial and three experimental) on oral mesenchymal stem cells (MSCs) and the persistence of stemness properties in high passage MSCs. Sorption and solubility tests were made for all materials. MSCs were isolated from re-entry palatal and periodontal granulation tissues and were characterized and cultured on composite discs. Cytotoxicity of the materials was evaluated by the Alamar Blue viability test, by Paul Karl Horan (PKH) labeling, and by immunocytochemical staining for actin. Water and saliva sorption and solubility data revealed that two of the experimental materials behaved comparable with the marketed resin composites. The Alamar Blue viability test shows that both cell lines grew well on composite discs that seemed to induce no apparen...
Genetic polymorphisms in the vitamin D receptor gene (VDR) were related to bone mineral density, ... more Genetic polymorphisms in the vitamin D receptor gene (VDR) were related to bone mineral density, bone turnover, and diseases with bone loss, as in periodontitis. RUNX2 regulates transcription of numerous genes and thereby control osteoblast development from mesenchymal stem cells and maturation into osteocytes. During osteoblast development and maturation, RUNX2 cooperates also with other transcription factors and cofactors. Mutations in this gene have been associated with cleidocranial dysplasia, a bone development disorder. Mutations in the PAX9 gene were frequently associated with oligodontia or hypodontia, as well as with Class II/Division 2 malocclusions (with or without hypodontia). The aim of the present paper was to identify genes which we presume to be involved in the development of the craniofacial complex. We selected three markers: VDR (vitamin D receptor - rs731236), PAX9 (rs8004560) and RUNX2 (rs731236) in order to reveal their association with Class I, II and III dent...
Background. The Matrilin-1 gene is thought to be involved in the formation of the filamentous net... more Background. The Matrilin-1 gene is thought to be involved in the formation of the filamentous network of the cartilage’s extracellular matrix and implicitly in the formation of the mandibular condyle cartilage, very important in different malocclusions. Objectives. The purpose of this study was the association of the marker Myo1H (rs10850110) with malocclusions. Methods. The sample comprises 47 patients with different malocclusions. The control group included 11 individuals. Cephalometric measurements were performed. The genotyping was done by sequencing. Results. Significant differences concerning allele and genotype distribution were observed between different malocclusions and the control group. The genotypes were distributed in the population according to Hardy-Weinberg equilibrium. Conclusions. Our findings are in agreement with other studies that emphasize the importance of the Myo1H gene in the determination of Class I, II and especially Class III phenotypes.
Third molar presence in the infratemporal fossa is a rare event and it has been reported previous... more Third molar presence in the infratemporal fossa is a rare event and it has been reported previously only two times in the literature, except for the cases which arise from complications occurring during the extraction of the impacted upper third molar. Due to the presence of important vessel bundles and nerves in this area, third molar removal requires a correct surgical management in order to avoid many possible serious side effects. We report an unusual case of upper third molar detected in the infratemporal fossa, which has been thoroughly investigated radiologically and removed through a safe surgical approach.
Revista medico-chirurgicală̆ a Societă̆ţ̜ii de Medici ş̧i Naturaliş̧ti din Iaş̧i
In the era of fixed appliances, some orthodontic practitioners seem to forget about functional th... more In the era of fixed appliances, some orthodontic practitioners seem to forget about functional therapy. Functional appliances are the only capable of orthopedically changes during the growth spurt. Activators of all types, classic or opened, are elected appliances in growing subjects with class II/1 anomalies. The appropriate case selection, along with patient compliance, lead to improved facial aesthetic and dental occlusion, at affordable prices. With this case presentation, we want to show our protocol in the management of these cases.
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