(a) Detection of NaStEP in different genetic backgrounds. Total RNA (10 μg) from mature pistils o... more (a) Detection of NaStEP in different genetic backgrounds. Total RNA (10 μg) from mature pistils of SI () and SC (), (), and () was blotted and hybridized with P-labelled . (b) expression in developing anthers and pistils of . Total RNA (10 μg) was loaded in each lane, blotted, and probed with P-labelled . (c) expression in developing anthers and pistils of . Total RNA (10 μg) was loaded in each lane, blotted, and probed with P-labelled . (d) Densitometric analysis of and expression during the development of styles plus stigmas. Numbers 1–5 represents the developmental stages of stigmas plus styles: 1, 0.5–1.0 cm; 2, 1–2 cm; 3, 2–3.5 cm; 4, 3.5–6 cm; and 5, 6 cm. (e) and expression in non-sexual organs of . Total RNA (10 μg) was loaded in each lane, blotted, and probed with a P-labelled (upper) or (lower). The same amount of stigma RNA was loaded as hybridization control. To ascertain equal RNA loading, blots were stained with methylene blue (lower sections of a–c and e).<b>Copyright information:</b>Taken from "Pollination in stimulates synthesis and transfer to the stigmatic surface of NaStEP, a vacuolar Kunitz proteinase inhibitor homologue"Journal of Experimental Botany 2008;59(11):3187-3201.Published online Jan 2008PMCID:PMC2504342.
Lacandonia schismatica, a mycoheterotrophic, hermaphroditic monocotyledon endemic to the Lacandon... more Lacandonia schismatica, a mycoheterotrophic, hermaphroditic monocotyledon endemic to the Lacandon rain forest of southeast Mexico, is the only flowering plant for which a spatial inversion (heterotopy, complete homeosis) of the reproductive floral whorls (stamens and carpels) ...
This study explores whether ecological factors, such as pollinators and pollen flow, or variation... more This study explores whether ecological factors, such as pollinators and pollen flow, or variation in pollen and ovule development account for the observed differences (approximately twofold) in the reproductive output of pin and thrum individuals of Erythroxylum havanense. The importance of ecological factors was assessed by means of comparison of the identity of pollinators and the rates of flower visitation, and by performing controlled hand pollinations and measurements of fruit set. In addition, we described the pollen and ovule development of thrum and pin individuals. Our results indicate that pollinators of E. havanense do not distinguish between floral morphs. The differences in fruit set between pin and thrum plants held even after hand pollination and, therefore, the observed differences in reproductive output between floral morphs of E. havanense cannot be explained in terms of asymmetrical pollen flow. There were no differences in the pattern of gynoecium development between the pin and thrum morphs, however androecium development showed marked differences between the morphs, and there was a resemblance between the developmental pathways leading to male sterility of the thrum morph of E. havanense with that of species with cytoplasmic male sterility (CMS).
Total protein from exudate of unpollinated and pollinated stigmas of cv Breakthrough were sequent... more Total protein from exudate of unpollinated and pollinated stigmas of cv Breakthrough were sequentially extracted, separated by SDS–PAGE, and blotted to nitrocellulose for immunostaining with anti-NaStEP (top) or silver nitrate stained (bottom). Equal volumes (30 μl) were loaded in each lane. (a) Total protein from exudate of unpollinated stigmas at anthesis and at 48 h old. (b) Total proteins from exudate after 5, 12, and 24 h of self-pollination. (c) Total proteins from exudate after 5, 12, and 24 h of pollination with pollen. (d), (e), and (f) Densitometric analysis of NaStEP expression and its presence in the stigmatic exudate of before and after pollination with self- and pollen. L, low-salt wash; H, high-salt wash; T, proteins remaining in the stigma after washes.<b>Copyright information:</b>Taken from "Pollination in stimulates synthesis and transfer to the stigmatic surface of NaStEP, a vacuolar Kunitz proteinase inhibitor homologue"Journal of Experiment...
Unpollinated stigma from SC cv Breakthrough showing NaStEP localization in vacuoles and osmiophil... more Unpollinated stigma from SC cv Breakthrough showing NaStEP localization in vacuoles and osmiophilic bodies. (a) Cross-section of mature papillae surrounded by copious exudate containing secretory droplets. Arrows show cell wall interruptions. (b) A papillary cell with a large vacuole and osmiophilic bodies. (c) Anti-NaStEP labelling of vacuolar electron-dense bodies. Arrows show immunogold secondary antibody labelling. cv Breakthrough stigma 10 h after pollination with pollen. (d) Degenerating papillary cells (arrow), showing abundant exudate and a disordered cytoplasm with abnormal organelles and disintegrated vacuoles. (e) Vacuole showing poor anti-NaStEP labelling of the osmiophilic bodies (arrows). (f) A close-up of the labelling shown in (e) (black arrows) showing anti-NaStEP labelling (white arrows) in smaller osmiophilic bodies. Stigmatic exudate of cv Breakthrough. (g) and (h) Diagrams of a stigma plus style of and a transverse section of a stigma. The arrow and square show ...
(a) Total proteins (10 μg) from stigmas and styles of SI , SI , SI , SI , SI , SC , SC Breakthrou... more (a) Total proteins (10 μg) from stigmas and styles of SI , SI , SI , SI , SI , SC , SC Breakthrough, SI Hybrid 1 (SC SI ), SI Hybrid 2 (SC SI ), SC , SC , SC , and SC were separated by SDS–PAGE and either Coomassie blue stained (left) or blotted to nitrocellulose for immunostaining with anti-NaStEP antibody (right). (b) Cross-sections of Breakthrough, SI SS, SI , SC , SC , SC , and SC stigmas immunostained with anti-NaStEP. I and II: diagrams of a stigma plus style of and a transverse section of a stigma. The arrow and square show zones where images were taken. UTT, upper transmitting tissue; SC, secretory cells; PC, papillae cell. Bars, 50 mm.<b>Copyright information:</b>Taken from "Pollination in stimulates synthesis and transfer to the stigmatic surface of NaStEP, a vacuolar Kunitz proteinase inhibitor homologue"Journal of Experimental Botany 2008;59(11):3187-3201.Published online Jan 2008PMCID:PMC2504342.
Total protein (50 μg) from matures styles plus stigmas of SI were fractionated by two-dimensional... more Total protein (50 μg) from matures styles plus stigmas of SI were fractionated by two-dimensional electrophoresis. (a) Proteins transferred onto nitrocellulose and immunodetected with anti-NaStEP antibody. (b) Proteins blotted and immunodetected with the anti-S-RNase antibody. (c) Mature proteins silver stained.<b>Copyright information:</b>Taken from "Pollination in stimulates synthesis and transfer to the stigmatic surface of NaStEP, a vacuolar Kunitz proteinase inhibitor homologue"Journal of Experimental Botany 2008;59(11):3187-3201.Published online Jan 2008PMCID:PMC2504342.
Equal amounts of total protein (10 μg) of roots, stems, leaves, sepals, petals, pistils, styles, ... more Equal amounts of total protein (10 μg) of roots, stems, leaves, sepals, petals, pistils, styles, stigmas, ovaries, and anthers were separated by SDS–PAGE. (a) Proteins transferred onto nitrocellulose and immunostained with anti-NaStEP antibody. (b) Coomassie blue-stained proteins.<b>Copyright information:</b>Taken from "Pollination in stimulates synthesis and transfer to the stigmatic surface of NaStEP, a vacuolar Kunitz proteinase inhibitor homologue"Journal of Experimental Botany 2008;59(11):3187-3201.Published online Jan 2008PMCID:PMC2504342.
The black boxes under the alignment indicate the degree of sequence consensus. Arrowheads indicat... more The black boxes under the alignment indicate the degree of sequence consensus. Arrowheads indicate the potential signal peptide cleavage sites. Cysteine residues are marked with an asterisk. Putative glycosylation sites are single underlined. Presumed vacuolar sorting signals are boxed. The antigenic region is double underlined. A dash in the sequence indicates a gap introduced to maintain a good alignment.<b>Copyright information:</b>Taken from "Pollination in stimulates synthesis and transfer to the stigmatic surface of NaStEP, a vacuolar Kunitz proteinase inhibitor homologue"Journal of Experimental Botany 2008;59(11):3187-3201.Published online Jan 2008PMCID:PMC2504342.
Antecedentes: Thelocactus leucacanthus ssp. schmollii (Cactaceae) tiene una distribución restring... more Antecedentes: Thelocactus leucacanthus ssp. schmollii (Cactaceae) tiene una distribución restringida al estado de Querétaro y está categorizada como un taxón de preocupación menor en la lista roja de la IUCN y en el apéndice II de la CITES, pero no se encuentra en ninguna categoría en la NOM-059-SEMARNAT-2010. Preguntas: ¿Cuál es el sistema de cruza y de apareamiento de T. leucacanthus ssp? schmollii? ¿Qué recompensas florales ofrece a sus visitantes y cuáles son sus potenciales polinizadores? Especie de estudio: Thelocactus leucacanthus ssp. schmollii (Cactaceae). Sitio de estudio y fechas: Peñamiller, Querétaro, México en 2018. Métodos: Se realizaron tratamientos de polinización controlada para determinar el sistema de cruza, estableciéndose el porcentaje de frutos y promedio de semillas que se formaron por cada tratamiento. Se determinó el índice de entrecruza de Cruden (IEC). Se realizó el estudio de la morfo-anatomía del nectario y se realizaron observaciones de los visitantes ...
(a) Detection of NaStEP in different genetic backgrounds. Total RNA (10 μg) from mature pistils o... more (a) Detection of NaStEP in different genetic backgrounds. Total RNA (10 μg) from mature pistils of SI () and SC (), (), and () was blotted and hybridized with P-labelled . (b) expression in developing anthers and pistils of . Total RNA (10 μg) was loaded in each lane, blotted, and probed with P-labelled . (c) expression in developing anthers and pistils of . Total RNA (10 μg) was loaded in each lane, blotted, and probed with P-labelled . (d) Densitometric analysis of and expression during the development of styles plus stigmas. Numbers 1–5 represents the developmental stages of stigmas plus styles: 1, 0.5–1.0 cm; 2, 1–2 cm; 3, 2–3.5 cm; 4, 3.5–6 cm; and 5, 6 cm. (e) and expression in non-sexual organs of . Total RNA (10 μg) was loaded in each lane, blotted, and probed with a P-labelled (upper) or (lower). The same amount of stigma RNA was loaded as hybridization control. To ascertain equal RNA loading, blots were stained with methylene blue (lower sections of a–c and e).<b>Copyright information:</b>Taken from "Pollination in stimulates synthesis and transfer to the stigmatic surface of NaStEP, a vacuolar Kunitz proteinase inhibitor homologue"Journal of Experimental Botany 2008;59(11):3187-3201.Published online Jan 2008PMCID:PMC2504342.
Lacandonia schismatica, a mycoheterotrophic, hermaphroditic monocotyledon endemic to the Lacandon... more Lacandonia schismatica, a mycoheterotrophic, hermaphroditic monocotyledon endemic to the Lacandon rain forest of southeast Mexico, is the only flowering plant for which a spatial inversion (heterotopy, complete homeosis) of the reproductive floral whorls (stamens and carpels) ...
This study explores whether ecological factors, such as pollinators and pollen flow, or variation... more This study explores whether ecological factors, such as pollinators and pollen flow, or variation in pollen and ovule development account for the observed differences (approximately twofold) in the reproductive output of pin and thrum individuals of Erythroxylum havanense. The importance of ecological factors was assessed by means of comparison of the identity of pollinators and the rates of flower visitation, and by performing controlled hand pollinations and measurements of fruit set. In addition, we described the pollen and ovule development of thrum and pin individuals. Our results indicate that pollinators of E. havanense do not distinguish between floral morphs. The differences in fruit set between pin and thrum plants held even after hand pollination and, therefore, the observed differences in reproductive output between floral morphs of E. havanense cannot be explained in terms of asymmetrical pollen flow. There were no differences in the pattern of gynoecium development between the pin and thrum morphs, however androecium development showed marked differences between the morphs, and there was a resemblance between the developmental pathways leading to male sterility of the thrum morph of E. havanense with that of species with cytoplasmic male sterility (CMS).
Total protein from exudate of unpollinated and pollinated stigmas of cv Breakthrough were sequent... more Total protein from exudate of unpollinated and pollinated stigmas of cv Breakthrough were sequentially extracted, separated by SDS–PAGE, and blotted to nitrocellulose for immunostaining with anti-NaStEP (top) or silver nitrate stained (bottom). Equal volumes (30 μl) were loaded in each lane. (a) Total protein from exudate of unpollinated stigmas at anthesis and at 48 h old. (b) Total proteins from exudate after 5, 12, and 24 h of self-pollination. (c) Total proteins from exudate after 5, 12, and 24 h of pollination with pollen. (d), (e), and (f) Densitometric analysis of NaStEP expression and its presence in the stigmatic exudate of before and after pollination with self- and pollen. L, low-salt wash; H, high-salt wash; T, proteins remaining in the stigma after washes.<b>Copyright information:</b>Taken from "Pollination in stimulates synthesis and transfer to the stigmatic surface of NaStEP, a vacuolar Kunitz proteinase inhibitor homologue"Journal of Experiment...
Unpollinated stigma from SC cv Breakthrough showing NaStEP localization in vacuoles and osmiophil... more Unpollinated stigma from SC cv Breakthrough showing NaStEP localization in vacuoles and osmiophilic bodies. (a) Cross-section of mature papillae surrounded by copious exudate containing secretory droplets. Arrows show cell wall interruptions. (b) A papillary cell with a large vacuole and osmiophilic bodies. (c) Anti-NaStEP labelling of vacuolar electron-dense bodies. Arrows show immunogold secondary antibody labelling. cv Breakthrough stigma 10 h after pollination with pollen. (d) Degenerating papillary cells (arrow), showing abundant exudate and a disordered cytoplasm with abnormal organelles and disintegrated vacuoles. (e) Vacuole showing poor anti-NaStEP labelling of the osmiophilic bodies (arrows). (f) A close-up of the labelling shown in (e) (black arrows) showing anti-NaStEP labelling (white arrows) in smaller osmiophilic bodies. Stigmatic exudate of cv Breakthrough. (g) and (h) Diagrams of a stigma plus style of and a transverse section of a stigma. The arrow and square show ...
(a) Total proteins (10 μg) from stigmas and styles of SI , SI , SI , SI , SI , SC , SC Breakthrou... more (a) Total proteins (10 μg) from stigmas and styles of SI , SI , SI , SI , SI , SC , SC Breakthrough, SI Hybrid 1 (SC SI ), SI Hybrid 2 (SC SI ), SC , SC , SC , and SC were separated by SDS–PAGE and either Coomassie blue stained (left) or blotted to nitrocellulose for immunostaining with anti-NaStEP antibody (right). (b) Cross-sections of Breakthrough, SI SS, SI , SC , SC , SC , and SC stigmas immunostained with anti-NaStEP. I and II: diagrams of a stigma plus style of and a transverse section of a stigma. The arrow and square show zones where images were taken. UTT, upper transmitting tissue; SC, secretory cells; PC, papillae cell. Bars, 50 mm.<b>Copyright information:</b>Taken from "Pollination in stimulates synthesis and transfer to the stigmatic surface of NaStEP, a vacuolar Kunitz proteinase inhibitor homologue"Journal of Experimental Botany 2008;59(11):3187-3201.Published online Jan 2008PMCID:PMC2504342.
Total protein (50 μg) from matures styles plus stigmas of SI were fractionated by two-dimensional... more Total protein (50 μg) from matures styles plus stigmas of SI were fractionated by two-dimensional electrophoresis. (a) Proteins transferred onto nitrocellulose and immunodetected with anti-NaStEP antibody. (b) Proteins blotted and immunodetected with the anti-S-RNase antibody. (c) Mature proteins silver stained.<b>Copyright information:</b>Taken from "Pollination in stimulates synthesis and transfer to the stigmatic surface of NaStEP, a vacuolar Kunitz proteinase inhibitor homologue"Journal of Experimental Botany 2008;59(11):3187-3201.Published online Jan 2008PMCID:PMC2504342.
Equal amounts of total protein (10 μg) of roots, stems, leaves, sepals, petals, pistils, styles, ... more Equal amounts of total protein (10 μg) of roots, stems, leaves, sepals, petals, pistils, styles, stigmas, ovaries, and anthers were separated by SDS–PAGE. (a) Proteins transferred onto nitrocellulose and immunostained with anti-NaStEP antibody. (b) Coomassie blue-stained proteins.<b>Copyright information:</b>Taken from "Pollination in stimulates synthesis and transfer to the stigmatic surface of NaStEP, a vacuolar Kunitz proteinase inhibitor homologue"Journal of Experimental Botany 2008;59(11):3187-3201.Published online Jan 2008PMCID:PMC2504342.
The black boxes under the alignment indicate the degree of sequence consensus. Arrowheads indicat... more The black boxes under the alignment indicate the degree of sequence consensus. Arrowheads indicate the potential signal peptide cleavage sites. Cysteine residues are marked with an asterisk. Putative glycosylation sites are single underlined. Presumed vacuolar sorting signals are boxed. The antigenic region is double underlined. A dash in the sequence indicates a gap introduced to maintain a good alignment.<b>Copyright information:</b>Taken from "Pollination in stimulates synthesis and transfer to the stigmatic surface of NaStEP, a vacuolar Kunitz proteinase inhibitor homologue"Journal of Experimental Botany 2008;59(11):3187-3201.Published online Jan 2008PMCID:PMC2504342.
Antecedentes: Thelocactus leucacanthus ssp. schmollii (Cactaceae) tiene una distribución restring... more Antecedentes: Thelocactus leucacanthus ssp. schmollii (Cactaceae) tiene una distribución restringida al estado de Querétaro y está categorizada como un taxón de preocupación menor en la lista roja de la IUCN y en el apéndice II de la CITES, pero no se encuentra en ninguna categoría en la NOM-059-SEMARNAT-2010. Preguntas: ¿Cuál es el sistema de cruza y de apareamiento de T. leucacanthus ssp? schmollii? ¿Qué recompensas florales ofrece a sus visitantes y cuáles son sus potenciales polinizadores? Especie de estudio: Thelocactus leucacanthus ssp. schmollii (Cactaceae). Sitio de estudio y fechas: Peñamiller, Querétaro, México en 2018. Métodos: Se realizaron tratamientos de polinización controlada para determinar el sistema de cruza, estableciéndose el porcentaje de frutos y promedio de semillas que se formaron por cada tratamiento. Se determinó el índice de entrecruza de Cruden (IEC). Se realizó el estudio de la morfo-anatomía del nectario y se realizaron observaciones de los visitantes ...
Uploads
Papers by Sonia Vázquez Santana