Pelita Perkebunan (a Coffee and Cocoa Research Journal)
Caffeine could be utilized by caffeine-degrading bacteria as a source of carbon and nitrogen. The... more Caffeine could be utilized by caffeine-degrading bacteria as a source of carbon and nitrogen. These bacteria have the potential as an agent of decaffeinating coffee. The objective of this research was to characterize and identify the caffeinedegrading bacteria KAJ 36 that was isolated from the pulp waste of Coffea arabica. The isolate-characterization was performed based on its growth and caffeine degradation activity.in a medium M9 added with 10 g/L caffeine for 7 days. The isolate identification was conducted using 16S rRNA sequence analysis and biochemical analysis. The result indicated that caffein-degrading bacteria were able to grow and degrade the caffein up to 86% activity on M9 medium added 10 g/L caffeine for 7 days incubation. Based on 16S rRNA, the isolated KAJ 36 had sequences of 99% homology with Pseudomonas monteilii. Biochemical analysis showed that this isolation on the specific medium was a gram-negative, oxidative, positive enzymatic activity (oxidase, catalase, u...
Pelita Perkebunan (a Coffee and Cocoa Research Journal)
Caffeine could be utilized by caffeine-degrading bacteria as a source of carbon and nitrogen. The... more Caffeine could be utilized by caffeine-degrading bacteria as a source of carbon and nitrogen. These bacteria have the potential as an agent of decaffeinating coffee. The objective of this research was to characterize and identify the caffeinedegrading bacteria KAJ 36 that was isolated from the pulp waste of Coffea arabica. The isolate-characterization was performed based on its growth and caffeine degradation activity.in a medium M9 added with 10 g/L caffeine for 7 days. The isolate identification was conducted using 16S rRNA sequence analysis and biochemical analysis. The result indicated that caffein-degrading bacteria were able to grow and degrade the caffein up to 86% activity on M9 medium added 10 g/L caffeine for 7 days incubation. Based on 16S rRNA, the isolated KAJ 36 had sequences of 99% homology with Pseudomonas monteilii. Biochemical analysis showed that this isolation on the specific medium was a gram-negative, oxidative, positive enzymatic activity (oxidase, catalase, u...
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