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Andreina Schoeberlein

University of Bern, Department for BioMedical Research, Medical Faculty, Faculty Member

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Scientist with a background in animal husbandry, breeding, and genomics. Basic and applied science in pre-/perinatal medicine, neuroscience, and the cardiovascular field. Focus on the development of stem cell-based therapies for the treatment of perinatal brain injury.

Specialties: stem cells, stem cell-based therapies, neuroscience, regenerative medicine, animal models, cell biology, molecular biology, hypoxia, ischemia-reperfusion injury,tissue engineering, genomics
Address: Bern, Switzerland

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Papers by Andreina Schoeberlein

294: Umbilical cord stem cells prime neural progenitor cells towards an oligodendrocyte fate

American Journal of Obstetrics and Gynecology, 2015

Human Wharton's Jelly Mesenchymal Stromal Cell-Derived Small Extracellular Vesicles Drive Oligodendroglial Maturation by Restraining MAPK/ERK and Notch Signaling Pathways

Front Cell Dev Biol, 2021

Cord sEV Promote OPC Maturation having key roles in the MAPK pathway. Our data strongly suggest t... more

Human Wharton's Jelly Mesenchymal Stromal Cell-Derived Small Extracellular Vesicles Drive Oligodendroglial Maturation by Restraining MAPK/ERK and Notch Signaling Pathways

Front Cell Dev Biol, 2021

Peripartum cerebral hypoxia and ischemia, and intrauterine infection and inflammation, are detrim... more Peripartum cerebral hypoxia and ischemia, and intrauterine infection and inflammation, are detrimental for the precursor cells of the myelin-forming oligodendrocytes in the prematurely newborn, potentially leading to white matter injury (WMI) with long-term neurodevelopmental sequelae. Previous data show that hypomyelination observed in WMI is caused by arrested oligodendroglial maturation rather than oligodendrocyte-specific cell death. In a rat model of premature WMI, we have recently shown that small extracellular vesicles (sEV) derived from Wharton's jelly mesenchymal stromal cells (WJ-MSC) protect from myelination deficits. Thus, we hypothesized that sEV derived from WJ-MSC directly promote oligodendroglial maturation in oligodendrocyte precursor cells. To test this assumption, sEV were isolated from culture supernatants of human WJ-MSC by ultracentrifugation and co-cultured with the human immortalized oligodendrocyte precursor cell line MO3.13. As many regulatory functions in WMI have been ascribed to microRNA (miR) and as sEV are carriers of functional miR which can be delivered to target cells, we characterized and quantified the miR content of WJ-MSC-derived sEV by next-generation sequencing. We found that WJ-MSC-derived sEV co-localized with MO3.13 cells within 4 h. After 5 days of co-culture, the expression of myelin basic protein (MBP), a marker for mature oligodendrocytes, was significantly increased, while the oligodendrocyte precursor marker platelet-derived growth factor alpha (PDGFRα) was decreased. Notch and MAPK/ERK pathways known to inhibit oligodendrocyte maturation and differentiation were significantly reduced. The pathway enrichment analysis showed that the miR present in WJ-MSC-derived sEV target genes

Scientific Abstracts

Extracellular Vesicles Derived from Wharton's Jelly Mesenchymal Stem Cells Prevent and Resolve Programmed Cell Death Mediated by Perinatal Hypoxia-Ischemia in Neuronal Cells

F-207 Effects of Estrogen on Proliferative Activity of Murine Female Bone Marrow Stromal Cells. Seung- F-209 The Potential Oligodendroglial Fate Specification Capacity of Exosomes Derived from Stem Cells of the Umbilical Cord Tissue

Mesenchymal Stem Cells from Wharton's Jelly and Amniotic Fluid

World Journal of Stem Cells

Reduction of Soluble E-Selectin after Combined Treatment with Simvastatin and Vitamins C and E

Heart Drug, 2005

Stammzelltransplantation in einem neuen Rattenmodell schwerwiegender perinataler Hirnschädigung

Intrakraniale Stammzelltransplantation mit und ohne Erythropoietin erleichert die motorische Wiederherstellung bei perinataler Hirnschädigung im Rattenmodell

Zeitschrift für Geburtshilfe und Neonatologie, 2012

Placenta-derived Mesenchymal Stem Cells for the Neuroregeneration in a Rat Perinatal Brain Injury Model

Zeitschrift für Geburtshilfe und Neonatologie, 2011

Placental mesenchymal stem cells as potential grafts for peripartum osteogenic and neural regeneration

Ultraschall in der Medizin - European Journal of Ultrasound, 2008

The importance of endothelial adhesion molecules in cardiac surgery[Die bedeutung der endothelialen adhasions-molekule in der herzchirurgie]

Swiss Surgery

ABSTRACT

Mesenchymal Stem Cells from Wharton’s Jelly and Amniotic Fluid

Best Practice & Research Clinical Obstetrics & Gynaecology, 2015

The discovery of mesenchymal stem cells (MSCs) in perinatal sources, such as the amniotic fluid (... more The discovery of mesenchymal stem cells (MSCs) in perinatal sources, such as the amniotic fluid (AF) and the umbilical connective tissue, the so-called Wharton&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s jelly (WJ), has transformed them into promising stem cell grafts for the application in regenerative medicine. The advantages of AF-MSCs and WJ-MSCs over adult MSCs, such as bone marrow-derived mesenchymal stem cells (BM-MSCs), include their minimally invasive isolation procedure, their more primitive cell character without being tumourigenic, their low immunogenicity and their potential autologous application in congenital disorders and when cryopreserved in adulthood. This chapter gives an overview of the biology of AF-MSCs and WJ-MSCs, and their regenerative potential based on the results of recent preclinical and clinical studies. In the end, open questions concerning the use of WJ-MSCs and AF-MSCs in regenerative medicine will be emphasized.

Intrakraniale Stammzelltransplantation erleichtert die motorische Wiederherstellung in einem Rattenmodell der perinatalen Hirnschädigung

Zeitschrift für Geburtshilfe und Neonatologie, 2013

Homing und Engraftment von autologen versus allogenen Stammzellen nach intrauteriner Transplantation im fetalen Schaf

Geburtshilfe und Frauenheilkunde, 2003

53: Successful transnasal delivery of stem cells in a rat model of perinatal hypoxic-ischemic brain injury

American Journal of Obstetrics and Gynecology, 2015

Optimized growth conditions for tissue engineering of human cardiovascular structures

The International journal of artificial organs, 2000

Optimized in vitro formation of strong tissue is a prerequisite for tissue engineering of cardiov... more Optimized in vitro formation of strong tissue is a prerequisite for tissue engineering of cardiovascular structures, such as heart valves and blood vessels. This study evaluates different growth media additives as to cell proliferation, extracellular matrix formation, and mechanical characteristics. Biodegradable polymers were seeded with human vascular myofibroblasts. Group A was cultured with standard medium, groups B, C, and D were in addition supplemented with ascorbate, fibroblast growth factor (bFGF), and both respectively. Analysis included histology, electron microsocopy, mechanical testing, and biochemical assays for cell proliferation (DNA) and extracellular matrix (collagen). DNA content increased in all groups, showing significantly more cells in group C and D after 14d. Collagen increased in all groups, except for C. Morphology showed viable, layered cellular tissue, with collagen fibrils after 2w, most pronounced in B and D. Mechanical properties decreased initially, s...

294: Umbilical cord stem cells prime neural progenitor cells towards an oligodendrocyte fate

American Journal of Obstetrics and Gynecology, 2015

Human Wharton's Jelly Mesenchymal Stromal Cell-Derived Small Extracellular Vesicles Drive Oligodendroglial Maturation by Restraining MAPK/ERK and Notch Signaling Pathways

Front Cell Dev Biol, 2021

Cord sEV Promote OPC Maturation having key roles in the MAPK pathway. Our data strongly suggest t... more

Human Wharton's Jelly Mesenchymal Stromal Cell-Derived Small Extracellular Vesicles Drive Oligodendroglial Maturation by Restraining MAPK/ERK and Notch Signaling Pathways

Front Cell Dev Biol, 2021

Peripartum cerebral hypoxia and ischemia, and intrauterine infection and inflammation, are detrim... more Peripartum cerebral hypoxia and ischemia, and intrauterine infection and inflammation, are detrimental for the precursor cells of the myelin-forming oligodendrocytes in the prematurely newborn, potentially leading to white matter injury (WMI) with long-term neurodevelopmental sequelae. Previous data show that hypomyelination observed in WMI is caused by arrested oligodendroglial maturation rather than oligodendrocyte-specific cell death. In a rat model of premature WMI, we have recently shown that small extracellular vesicles (sEV) derived from Wharton's jelly mesenchymal stromal cells (WJ-MSC) protect from myelination deficits. Thus, we hypothesized that sEV derived from WJ-MSC directly promote oligodendroglial maturation in oligodendrocyte precursor cells. To test this assumption, sEV were isolated from culture supernatants of human WJ-MSC by ultracentrifugation and co-cultured with the human immortalized oligodendrocyte precursor cell line MO3.13. As many regulatory functions in WMI have been ascribed to microRNA (miR) and as sEV are carriers of functional miR which can be delivered to target cells, we characterized and quantified the miR content of WJ-MSC-derived sEV by next-generation sequencing. We found that WJ-MSC-derived sEV co-localized with MO3.13 cells within 4 h. After 5 days of co-culture, the expression of myelin basic protein (MBP), a marker for mature oligodendrocytes, was significantly increased, while the oligodendrocyte precursor marker platelet-derived growth factor alpha (PDGFRα) was decreased. Notch and MAPK/ERK pathways known to inhibit oligodendrocyte maturation and differentiation were significantly reduced. The pathway enrichment analysis showed that the miR present in WJ-MSC-derived sEV target genes

Scientific Abstracts

Extracellular Vesicles Derived from Wharton's Jelly Mesenchymal Stem Cells Prevent and Resolve Programmed Cell Death Mediated by Perinatal Hypoxia-Ischemia in Neuronal Cells

F-207 Effects of Estrogen on Proliferative Activity of Murine Female Bone Marrow Stromal Cells. Seung- F-209 The Potential Oligodendroglial Fate Specification Capacity of Exosomes Derived from Stem Cells of the Umbilical Cord Tissue

Mesenchymal Stem Cells from Wharton's Jelly and Amniotic Fluid

World Journal of Stem Cells

Reduction of Soluble E-Selectin after Combined Treatment with Simvastatin and Vitamins C and E

Heart Drug, 2005

Stammzelltransplantation in einem neuen Rattenmodell schwerwiegender perinataler Hirnschädigung

Intrakraniale Stammzelltransplantation mit und ohne Erythropoietin erleichert die motorische Wiederherstellung bei perinataler Hirnschädigung im Rattenmodell

Zeitschrift für Geburtshilfe und Neonatologie, 2012

Placenta-derived Mesenchymal Stem Cells for the Neuroregeneration in a Rat Perinatal Brain Injury Model

Zeitschrift für Geburtshilfe und Neonatologie, 2011

Placental mesenchymal stem cells as potential grafts for peripartum osteogenic and neural regeneration

Ultraschall in der Medizin - European Journal of Ultrasound, 2008

The importance of endothelial adhesion molecules in cardiac surgery[Die bedeutung der endothelialen adhasions-molekule in der herzchirurgie]

Swiss Surgery

ABSTRACT

Mesenchymal Stem Cells from Wharton’s Jelly and Amniotic Fluid

Best Practice & Research Clinical Obstetrics & Gynaecology, 2015

The discovery of mesenchymal stem cells (MSCs) in perinatal sources, such as the amniotic fluid (... more The discovery of mesenchymal stem cells (MSCs) in perinatal sources, such as the amniotic fluid (AF) and the umbilical connective tissue, the so-called Wharton&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s jelly (WJ), has transformed them into promising stem cell grafts for the application in regenerative medicine. The advantages of AF-MSCs and WJ-MSCs over adult MSCs, such as bone marrow-derived mesenchymal stem cells (BM-MSCs), include their minimally invasive isolation procedure, their more primitive cell character without being tumourigenic, their low immunogenicity and their potential autologous application in congenital disorders and when cryopreserved in adulthood. This chapter gives an overview of the biology of AF-MSCs and WJ-MSCs, and their regenerative potential based on the results of recent preclinical and clinical studies. In the end, open questions concerning the use of WJ-MSCs and AF-MSCs in regenerative medicine will be emphasized.

Intrakraniale Stammzelltransplantation erleichtert die motorische Wiederherstellung in einem Rattenmodell der perinatalen Hirnschädigung

Zeitschrift für Geburtshilfe und Neonatologie, 2013

Homing und Engraftment von autologen versus allogenen Stammzellen nach intrauteriner Transplantation im fetalen Schaf

Geburtshilfe und Frauenheilkunde, 2003

53: Successful transnasal delivery of stem cells in a rat model of perinatal hypoxic-ischemic brain injury

American Journal of Obstetrics and Gynecology, 2015

Optimized growth conditions for tissue engineering of human cardiovascular structures

The International journal of artificial organs, 2000

Optimized in vitro formation of strong tissue is a prerequisite for tissue engineering of cardiov... more Optimized in vitro formation of strong tissue is a prerequisite for tissue engineering of cardiovascular structures, such as heart valves and blood vessels. This study evaluates different growth media additives as to cell proliferation, extracellular matrix formation, and mechanical characteristics. Biodegradable polymers were seeded with human vascular myofibroblasts. Group A was cultured with standard medium, groups B, C, and D were in addition supplemented with ascorbate, fibroblast growth factor (bFGF), and both respectively. Analysis included histology, electron microsocopy, mechanical testing, and biochemical assays for cell proliferation (DNA) and extracellular matrix (collagen). DNA content increased in all groups, showing significantly more cells in group C and D after 14d. Collagen increased in all groups, except for C. Morphology showed viable, layered cellular tissue, with collagen fibrils after 2w, most pronounced in B and D. Mechanical properties decreased initially, s...