ABSTRACT The semiempirical quantum mechanical MNDO and AM1 methods are employed to model Porter&a... more ABSTRACT The semiempirical quantum mechanical MNDO and AM1 methods are employed to model Porter's scheme for lipid peroxidation. The results obtained by the two methods, while supporting the proposed reaction pathway, show the expected differences in the structure of the investigated molecules.
Traditionally, in Mediterranean areas the goat population was composed of autochthonous breeds wi... more Traditionally, in Mediterranean areas the goat population was composed of autochthonous breeds with strong milk production seasonality. In the last decades, high productive alpine breeds were introduced together with more widespread out-of-season milk production practices. This study is a large-scale survey on the seasonal variations of the main compositional characteristics of goat milk obtained from Sarda and Saanen breeds reared on the Mediterranean island of Sardinia (Italy). Analysis of data indicated that milk from the Sarda breed was significantly richer, at p < 0.001, in protein, fat, and lactose, and had a lower urea mean content than Saanen. Throughout the year, fluctuations of mean contents of the milk parameters were similar for the two groups of goats, indicating that, besides genetic intrinsic differences, climate and herbage growth influenced the Sarda as well as the Saanen goats. During the summer, milk from Saanen showed a marked drop in fat and protein contents,...
Lactobacillus strains with the potential of protecting fresh dairy products from spoilage were st... more Lactobacillus strains with the potential of protecting fresh dairy products from spoilage were studied. Metabolism and antifungal activity of different L. plantarum, L. brevis, and L. sakei strains, isolated from Sardinian dairy and meat products, were assessed. The metabolite fingerprint of each strain was obtained by GC-MS and data submitted to multivariate statistical analysis. The discriminant analysis correctly classified samples to the Lactobacillus species and indicated that, with respect to the other species, L. plantarum had higher levels of organic acids, while L. brevis and L. sakei showed higher levels of sugars than L. plantarum. Partial Least Square (PLS) regression correlated the GC-MS metabolites to the antifungal activity (p < 0.05) of Lactobacillus strains and indicated that organic acids and oleamide are positively related with this ability. Some of the metabolites identified in this study have been reported to possess health promoting proprieties. These overal...
The supplementation of goat diets with natural products to obtain milk with nutraceutical compone... more The supplementation of goat diets with natural products to obtain milk with nutraceutical components is a common practice. In these last years, the influence of supplementation of specifically designed diets has been studied with different analytical tools in order to explore possible beneficial effects in human consumption of animal milk and milk-derived products. In this study, the lipid fraction of milk from Alpine goats undergoing different dietary regimens was studied by 1H-NMR spectroscopy. Alpine goats were fed with linseed or hempseed supplements, and after 14 weeks of treatment, milk was collected and analyzed. Results showed that feeding diets supplemented with seeds positively affected the fatty acid composition with a pronounced increase in unsaturated fatty acids for both diets compared to a control diet. Specifically, linolenic acid content was more than doubled for linseed diet compared with the hempseed and control groups, while linoleic acid greatly increased only u...
Sardinia has a long tradition in making a high quality mullet bottarga (Mugil cephalus). Sardinia... more Sardinia has a long tradition in making a high quality mullet bottarga (Mugil cephalus). Sardinian bottarga is the final product of a number of treatments (basically salting and drying) conducted on the ovaries of mullet. In this work main lipid classes, fatty acids and fatty alcohols compositions, and oxidative status of commercial samples of grated bottarga produced in Sardinia were studied by using different analytical techniques (GC, HPLC and 13C NMR). Wax esters resulted the main lipid fraction (>50 mol%), whereas triacylglycerols and phospholipids, cholesterol and cholesteryl-esters represented a minor fraction, with concentrations of free fatty acids in the range 15-29 mol%. The most represented fatty alcohols were 14:0, 16:0, 16:1 n-7 e 18:0, among fatty acids the most represented were 16:0, 18:0, 16:1 n-7, 18:1 n-9, 20:5 n-3 (EPA, 10-13 mg/g of the edible portion) and 22:6 n-3 (DHA, 20-29 mg/g). The oxidation status was measured by detection of conjugated dienes fatty ac...
The paper shows the modifications of the water-soluble low molecular weight metabolites extracted... more The paper shows the modifications of the water-soluble low molecular weight metabolites extracted from samples of salted and dried mullet roe stored at different temperatures. This molecular pool comprises free amino acids, organic acids, nucleotides and derivatives, sugars, volatile amines and others. The survey technique used is the 1H NMR spectroscopy in the liquid state. The roes were stored at -20°C, at 4°C, and at room temperature in dark and light exposure, for 7 months. The samples were examined at initial time and after 1, 2, 3, 5 and 7 months of storage. The multivariate analysis PCA applied to the spectral data indicated that samples stored at -20°C are very similar during time and exhibit opposite characteristics to those stored for 5 and 7 months at room temperature, darkness and light. The analysis of the integrated areas of the spectra indicated that the major compositional changes are: the increase of the derivatives of the breakdown of phosphatidylcholine (choline, ...
ABSTRACT The semiempirical quantum mechanical MNDO and AM1 methods are employed to model Porter&a... more ABSTRACT The semiempirical quantum mechanical MNDO and AM1 methods are employed to model Porter&#39;s scheme for lipid peroxidation. The results obtained by the two methods, while supporting the proposed reaction pathway, show the expected differences in the structure of the investigated molecules.
Traditionally, in Mediterranean areas the goat population was composed of autochthonous breeds wi... more Traditionally, in Mediterranean areas the goat population was composed of autochthonous breeds with strong milk production seasonality. In the last decades, high productive alpine breeds were introduced together with more widespread out-of-season milk production practices. This study is a large-scale survey on the seasonal variations of the main compositional characteristics of goat milk obtained from Sarda and Saanen breeds reared on the Mediterranean island of Sardinia (Italy). Analysis of data indicated that milk from the Sarda breed was significantly richer, at p < 0.001, in protein, fat, and lactose, and had a lower urea mean content than Saanen. Throughout the year, fluctuations of mean contents of the milk parameters were similar for the two groups of goats, indicating that, besides genetic intrinsic differences, climate and herbage growth influenced the Sarda as well as the Saanen goats. During the summer, milk from Saanen showed a marked drop in fat and protein contents,...
Lactobacillus strains with the potential of protecting fresh dairy products from spoilage were st... more Lactobacillus strains with the potential of protecting fresh dairy products from spoilage were studied. Metabolism and antifungal activity of different L. plantarum, L. brevis, and L. sakei strains, isolated from Sardinian dairy and meat products, were assessed. The metabolite fingerprint of each strain was obtained by GC-MS and data submitted to multivariate statistical analysis. The discriminant analysis correctly classified samples to the Lactobacillus species and indicated that, with respect to the other species, L. plantarum had higher levels of organic acids, while L. brevis and L. sakei showed higher levels of sugars than L. plantarum. Partial Least Square (PLS) regression correlated the GC-MS metabolites to the antifungal activity (p < 0.05) of Lactobacillus strains and indicated that organic acids and oleamide are positively related with this ability. Some of the metabolites identified in this study have been reported to possess health promoting proprieties. These overal...
The supplementation of goat diets with natural products to obtain milk with nutraceutical compone... more The supplementation of goat diets with natural products to obtain milk with nutraceutical components is a common practice. In these last years, the influence of supplementation of specifically designed diets has been studied with different analytical tools in order to explore possible beneficial effects in human consumption of animal milk and milk-derived products. In this study, the lipid fraction of milk from Alpine goats undergoing different dietary regimens was studied by 1H-NMR spectroscopy. Alpine goats were fed with linseed or hempseed supplements, and after 14 weeks of treatment, milk was collected and analyzed. Results showed that feeding diets supplemented with seeds positively affected the fatty acid composition with a pronounced increase in unsaturated fatty acids for both diets compared to a control diet. Specifically, linolenic acid content was more than doubled for linseed diet compared with the hempseed and control groups, while linoleic acid greatly increased only u...
Sardinia has a long tradition in making a high quality mullet bottarga (Mugil cephalus). Sardinia... more Sardinia has a long tradition in making a high quality mullet bottarga (Mugil cephalus). Sardinian bottarga is the final product of a number of treatments (basically salting and drying) conducted on the ovaries of mullet. In this work main lipid classes, fatty acids and fatty alcohols compositions, and oxidative status of commercial samples of grated bottarga produced in Sardinia were studied by using different analytical techniques (GC, HPLC and 13C NMR). Wax esters resulted the main lipid fraction (>50 mol%), whereas triacylglycerols and phospholipids, cholesterol and cholesteryl-esters represented a minor fraction, with concentrations of free fatty acids in the range 15-29 mol%. The most represented fatty alcohols were 14:0, 16:0, 16:1 n-7 e 18:0, among fatty acids the most represented were 16:0, 18:0, 16:1 n-7, 18:1 n-9, 20:5 n-3 (EPA, 10-13 mg/g of the edible portion) and 22:6 n-3 (DHA, 20-29 mg/g). The oxidation status was measured by detection of conjugated dienes fatty ac...
The paper shows the modifications of the water-soluble low molecular weight metabolites extracted... more The paper shows the modifications of the water-soluble low molecular weight metabolites extracted from samples of salted and dried mullet roe stored at different temperatures. This molecular pool comprises free amino acids, organic acids, nucleotides and derivatives, sugars, volatile amines and others. The survey technique used is the 1H NMR spectroscopy in the liquid state. The roes were stored at -20°C, at 4°C, and at room temperature in dark and light exposure, for 7 months. The samples were examined at initial time and after 1, 2, 3, 5 and 7 months of storage. The multivariate analysis PCA applied to the spectral data indicated that samples stored at -20°C are very similar during time and exhibit opposite characteristics to those stored for 5 and 7 months at room temperature, darkness and light. The analysis of the integrated areas of the spectra indicated that the major compositional changes are: the increase of the derivatives of the breakdown of phosphatidylcholine (choline, ...
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Papers by Paola Scano