A new efficient synthesis of racemic anatabine is reported. The title target was obtained in an e... more A new efficient synthesis of racemic anatabine is reported. The title target was obtained in an excellent overall yield of 70%, by a five-step synthesis, using cheap reagents and mild reaction conditions.
Background: C. arborea belonging to Araliaceae family is used traditionally to cure many alien d... more Background: C. arborea belonging to Araliaceae family is used traditionally to cure many alien diseases including gonorrhoeae infection, diarrhea, malaria, and diabetes mellitus. The plant has been examined on the basis of scientific in vitro and in vivo evaluations possessing the major pharmacological activities including antimicrobial, antibacterial, antihyperglycemic, antiplasmodial and anticancer properties. Aim of the study: In the present paper, we reported the isolation and characterization of secondary metabolites from the methanol extract of the stem bark of Cussonia arborea Hochst after a short review of the traditional and pharmacological studies done on this important medicinal plant. Materials and methods: MeOH extract of stem bark of C. arborea was suspended in water and successively extracted with EtOAc and n-BuOH. The EtOAc extract (18 g) was subjected to repeated column chromatography to yield seven (1-7) compounds. Their structures were determined by means of NMR...
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
Hymenocardia acida (H. acida) is an African well-known shrub recognized for numerous medicinal pr... more Hymenocardia acida (H. acida) is an African well-known shrub recognized for numerous medicinal properties, including its cancer management potential. The advent of nanotechnology in delivering bioactive medicinal plant extract with poor solubility has improved the drug delivery system, for a better therapeutic value of several drugs from natural origins. This study aimed to evaluate the anticancer properties of H. acida using human lung (H460), breast (MCF-7), and colon (HCT 116) cancer cell lines as well as the production, characterization, and cytotoxicity study of H. acida loaded into PLGA nanoparticles. Benchtop models of Saccharomyces cerevisiae and Raniceps ranninus were used for preliminary toxicity evaluation. Notable cytotoxic activity in benchtop models and human cancer cell lines was observed for H. acida crude extract. The PLGA nanoparticles loading H. acida had a size of about 200 nm and an association efficiency of above 60%, making them suitable to be delivered by dif...
Anti-10 deacetylbaccatin III (DAB) antibodies (IgY) were elicited in hens immunized with a succin... more Anti-10 deacetylbaccatin III (DAB) antibodies (IgY) were elicited in hens immunized with a succinyl-DAB/BSA conjugate and extracted from egg yolk. As shown by indirect competitive inhibition enzyme immunoassay (CIEIA), the addition of free-DAB competitively inhibited the binding of affinity purified anti-DAB IgY to DAB/BSA solid phase conjugated antigen. The assay enabled the detection of DAB in concentrations as low as 7.5ng/ml (13.7 nM DAB), whereas anti-DAB IgY did not react with taxol even at a concentration a thousand times higher. The structural requirements of the diterpenoid nucleus for binding to IgY were considered on the basis of the levels of cross-reaction found with 10 authentic taxanes. The results indicate that anti-DAB IgY represents the first high affinity antibody produced capable of recognizing the ring skeleton of taxol precursors.
A new efficient synthesis of racemic anatabine is reported. The title target was obtained in an e... more A new efficient synthesis of racemic anatabine is reported. The title target was obtained in an excellent overall yield of 70%, by a five-step synthesis, using cheap reagents and mild reaction conditions.
Background: C. arborea belonging to Araliaceae family is used traditionally to cure many alien d... more Background: C. arborea belonging to Araliaceae family is used traditionally to cure many alien diseases including gonorrhoeae infection, diarrhea, malaria, and diabetes mellitus. The plant has been examined on the basis of scientific in vitro and in vivo evaluations possessing the major pharmacological activities including antimicrobial, antibacterial, antihyperglycemic, antiplasmodial and anticancer properties. Aim of the study: In the present paper, we reported the isolation and characterization of secondary metabolites from the methanol extract of the stem bark of Cussonia arborea Hochst after a short review of the traditional and pharmacological studies done on this important medicinal plant. Materials and methods: MeOH extract of stem bark of C. arborea was suspended in water and successively extracted with EtOAc and n-BuOH. The EtOAc extract (18 g) was subjected to repeated column chromatography to yield seven (1-7) compounds. Their structures were determined by means of NMR...
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
Hymenocardia acida (H. acida) is an African well-known shrub recognized for numerous medicinal pr... more Hymenocardia acida (H. acida) is an African well-known shrub recognized for numerous medicinal properties, including its cancer management potential. The advent of nanotechnology in delivering bioactive medicinal plant extract with poor solubility has improved the drug delivery system, for a better therapeutic value of several drugs from natural origins. This study aimed to evaluate the anticancer properties of H. acida using human lung (H460), breast (MCF-7), and colon (HCT 116) cancer cell lines as well as the production, characterization, and cytotoxicity study of H. acida loaded into PLGA nanoparticles. Benchtop models of Saccharomyces cerevisiae and Raniceps ranninus were used for preliminary toxicity evaluation. Notable cytotoxic activity in benchtop models and human cancer cell lines was observed for H. acida crude extract. The PLGA nanoparticles loading H. acida had a size of about 200 nm and an association efficiency of above 60%, making them suitable to be delivered by dif...
Anti-10 deacetylbaccatin III (DAB) antibodies (IgY) were elicited in hens immunized with a succin... more Anti-10 deacetylbaccatin III (DAB) antibodies (IgY) were elicited in hens immunized with a succinyl-DAB/BSA conjugate and extracted from egg yolk. As shown by indirect competitive inhibition enzyme immunoassay (CIEIA), the addition of free-DAB competitively inhibited the binding of affinity purified anti-DAB IgY to DAB/BSA solid phase conjugated antigen. The assay enabled the detection of DAB in concentrations as low as 7.5ng/ml (13.7 nM DAB), whereas anti-DAB IgY did not react with taxol even at a concentration a thousand times higher. The structural requirements of the diterpenoid nucleus for binding to IgY were considered on the basis of the levels of cross-reaction found with 10 authentic taxanes. The results indicate that anti-DAB IgY represents the first high affinity antibody produced capable of recognizing the ring skeleton of taxol precursors.
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Papers by Luciano Barboni