The present study investigated the effects of long-term estradiol withdrawal (ovariectomy) on hyp... more The present study investigated the effects of long-term estradiol withdrawal (ovariectomy) on hypothalamic serotonin-1A (5-HT(1A)) receptor signaling. Changes in neuroendocrine responses to the 5-HT(1A) agonist 8-OH-DPAT and levels of G(z) protein in the hypothalamus were used to examine 5-HT(1A) receptor signaling. Five days following ovariectomy, rats received daily injections of either 2 microg of beta-estradiol 3-benzoate or vehicle (subcutaneously) for 2, 4 or 14 days. Twenty-four hours after the last injection, and 15 min prior to sacrifice, rats were injected with (+/-)8-OH-DPAT (50 micro;g/kg, s.c.) or saline. Estradiol treatment did not alter basal corticotropin (ACTH) or oxytocin levels. Injection of (+/-)8-OH-DPAT produced significant increases in plasma ACTH and oxytocin levels. In the vehicle-treated rats, hormone responses to 8-OH-DPAT were enhanced in rats that received injections for 14 days compared with rats that received injections for either 2 or 4 days. Estradiol treatment for 4 or 14 days blunted this enhanced ACTH response to 8-OH-DPAT, whereas the oxytocin response to 8-OH-DPAT was only blunted after 14 daily injections of beta-estradiol 3-benzoate. The treatment with beta-estradiol 3-benzoate (2 microg/rat) did not reduce membrane-associated G(z) protein levels in the paraventricular nucleus of the hypothalamus. Hence, the inhibitory influence of a low dose of beta-estradiol 3-benzoate on 5-HT(1A) receptor signaling in the hypothalamus is not accompanied by a change in the levels of G(z) protein in the paraventricular hypothalamic nucleus. Results from the present study indicate a supersensitivity of 5-HT(1A) receptors after withdrawal of estradiol and suggest that estradiol suppresses 5-HT(1A) receptor signaling.
A theoretical model is developed which allows for well-defined lenses to be formed with different... more A theoretical model is developed which allows for well-defined lenses to be formed with different dioptric properties using lasers that are known to ablate a variety of materials. With the theoretical development as a guide an experimental arrangement is defined for the ArF excimer laser and this arrangement is used to form lenses with highly smooth surfaces in three polymeric materials (polymethyl methacrylate, polycarbonate, and polyvinyl chloride) to test the validity of the theory. As part of the procedure to obtain these lenses, ablation curves (etch depth per pulse vs log of the laser fluence) were measured for each of these polymers and these curves are also reported. The experiments were extended to the rabbit cornea in which the corneal button was reshaped as part of keratomileusis surgery. This preliminary experiment resulted in a clear cornea with a smooth corneal surface and a measured change of 3 diopters in the treated eye.
Biochemistry and Cell Biology-biochimie Et Biologie Cellulaire, 2006
The oxidation theory proposes that LDL oxidation is an early event in atherosclerosis and that ox... more The oxidation theory proposes that LDL oxidation is an early event in atherosclerosis and that oxidized LDL contributes to atherogenesis in triggering inflammation. In contrast to the copper-modified LDL, there are few studies using myeloperoxidase-modified LDL (Mox-LDL) as an inflammation inducer. Our aim is to test whether Mox-LDL could constitute a specific inducer of the inflammatory response. Albumin, which is the most abundant protein in plasma and which is present to an identical concentration of LDL in the intima, was used for comparison. The secretion of IL-8 by endothelial cells (Ea.hy926) and TNF-alpha by monocytes (THP-1) was measured in the cell medium after exposure of these cells to native LDL, native albumin, Mox-LDL, or Mox-albumin. We observed that Mox-LDL induced a 1.5- and 2-fold increase (ANOVA; P < 0.001) in IL-8 production at 100 microg/mL and 200 microg/mL, respectively. The incubation of THP-1 cells with Mox-LDL (100 microg/mL) increased the production of TNF-alpha 2-fold over the control. Native LDL, albumin, and Mox-albumin showed no effect in either cellular types. The myeloperoxidase-modified LDL increase in cytokine release by endothelial and monocyte cells and by firing both local and systemic inflammation could induce atherogenesis and its development.
... THE YIELD POTENTIAL UTILIZATION OF SOME SUNFLOWER HYBRIDS IN DIFFERENT CROPYEARS Maria DAM - ... more ... THE YIELD POTENTIAL UTILIZATION OF SOME SUNFLOWER HYBRIDS IN DIFFERENT CROPYEARS Maria DAM - Peter PEPÒ University of Debrecen ... a higher Rubisco content could be one factor in conferring better acclimation and higher drought tolerance (Pankovic et al. ...
ABSTRACT— Hepatitis C virus (HCV) has been proposed to be a cofactor in the pathogenesis of cirrh... more ABSTRACT— Hepatitis C virus (HCV) has been proposed to be a cofactor in the pathogenesis of cirrhosis in patients with chronic alcoholism. The demonstration of a different liver histological pattern in anti-HCV positive patients might provide additional evidence. We studied 164 patients with chronic alcoholism, and histologically proven cirrhosis. For all of them, serum samples were collected at the time of a liver biopsy and stored at –80°C. Testing for anti-HCV antibodies was done using the Ortho Diagnostic Systems Anti-HCV ELISA test. Only reproducible results were considered positive. A semi-quantitative assessment of seven histological parameters was made independently on liver biopsy samples. In the study group, 29 patients (18%) had anti-HCV antibodies. When compared with anti-HCV negative patients, both groups had similar ALT and AST seric activities. Anti-HCV positive patients had a greater score of mononuclear cells infiltrate (0.71±0.57 vs 0.41 ±0.52; p<0.05) and a lesser score of alcoholic hepatitis (0.19 ±0.57 vs 0.74 ±0.74; p<0.005). The scores for steatosis, perisinusoidal and perinodular fibrosis, and hepatocellular necrosis were similar in the two groups. In anti-HCV positive patients, with a clearly positive recombinant immunobinding assay (RIBA, Chiron-Ortho Diagnostic Systems), a greater score for hepatic necrosis and a lesser one for fibrosis were demonstrated. Among the seven patients with active cirrhosis, six were anti-HCV positive. Therefore, HCV is likely to play a role in the pathogenesis of liver damage in a few patients with alcoholic cirrhosis, especially, those with active cirrhosis.
Canadian Journal of Anaesthesia-journal Canadien D Anesthesie, 2007
Objective A left-sided double lumen tube is recommended for one-lung ventilation for most thoraci... more Objective A left-sided double lumen tube is recommended for one-lung ventilation for most thoracic surgeries, but for certain indications, a right-sided double lumen tube (R-DLT) may be mandatory. Frequent malposition of R-DLTs has been reported. We propose an innovative modification of Mallinckrodt’s Broncho-Cath™ R-DLT consisting of an enlarged area of the lateral orifice, and studied the impact of this modification on the adequacy of R-DLT positioning. Methods Eighty adult patients scheduled for elective thoracic surgery were randomized into two groups: standard Broncho-Cath™ R-DLT, or modified Broncho-Cath™ R-DLT. After induction of anesthesia, the R-DLT was positioned using a fibreoptic bronchoscope. The position of the R-DLT was assessed on three occasions: with the patient supine (T1), then immediately following the patient’s transfer to the lateral position (T2), and after repositioning of the tube, when needed, with the patient in lateral position (T3). A score ranging from 1 to 4 was accorded to the relative position of the right upper lobe (RUL) orifice in relation to the origin of the RUL bronchus. Results The modified Broncho-Cath™ R-DLT was more frequently in an adequate position at T2: 77% vs 37% of patients (P = 0.0121), and easier to reposition at T3: 97% vs 74% of patients (P = 0.0109) in comparison to the standard Broncho-Cath™ R-DLT group. Conclusion These data suggest the superiority of the modified Broncho-Cath™ R-DLT compared to a standard Broncho-Cath™ R-DLT for optimal R-DLT positioning to facilitate one-lung ventilation during thoracic surgery. Objectif Une sonde double-lumière gauche est recommandée lors de la ventilation pulmonaire sélective pour la majorité des chirurgies thoraciques; toutefois, dans certaines indications, une sonde double-lumière droite (SDL-D) peut être requise. Il est fait état de la malposition fréquente des SDL-D. Nous proposons une modification de la SDL-D Broncho-Cath™ de Mallinckrodt qui consiste en une surface élargie de l’orifice latéral, et avons étudié l’impact de cette modification sur le bon positionnement de la SDL-D. Méthodes Quatre-vingts patients adultes devant subir une chirurgie thoracique réglée ont été randomisés en deux groupes : SDL-D Broncho-Cath™ standard, ou SDL-D Broncho-Cath™ modifiée. Après induction de l’anesthésie, la SDL-D a été positionnée à l’aide d’un bronchoscope à fibres optiques. La position de la SDL-D a été évaluée trois fois : le patient sur le dos (T1), puis immédiatement après le transfert du patient en position latérale (T2), et après le repositionnement du tube, lorsque requis, le patient toujours en position latérale (T3). Une note de 1 à 4 a été donnée à la position relative de l’orifice du lobe supérieur droit (LSD) par rapport à l’origine de la bronche lobaire supérieure droite. Résultats La SDL-D Broncho-Cath™ modifiée fut plus fréquemment dans une position adéquate en T2: 77 % vs 37 % des patients (P = 0,0121) et fut plus facile à repositionner en T3 : 97 %vs74% des patients (P = 0,0109) par rapport au groupe SDL-D Broncho-Cath™ standard. Conclusion Ces données suggèrent la supériorité de la SDL-D Broncho-Cath™ modifiée par rapport à la SDL-D Broncho-Cath™ standard pour un positionnement optimal de la SDL-D afin de faciliter la ventilation pulmonaire sélective pendant la chirurgie thoracique.
Examines the French and UK traditions of management training and education, and how these have sh... more Examines the French and UK traditions of management training and education, and how these have shaped organizational approaches to human resource development. Presents results from a Price Waterhouse Cranfield survey which shows data based on human resource issues ...
Both ageing and vitamin C (VC) deficiency result in immune defect. Since low serum and tissue lev... more Both ageing and vitamin C (VC) deficiency result in immune defect. Since low serum and tissue levels of VC are found in the elderly, we have in a placebo-controlled study, tested the effect of VC supplements (500 mg/day i.m. for 1 month) on various immune parameters. Indeed, VC enhances the proliferative response of T lymphocytes in vitro, and the tuberculin skin hypersensitivity in vivo. Neither the serum concentrations of IgA, IgG and IgM, nor the proportion of E-rosette-forming cells were modified. No significant change was observed in the placebo-treated group.
Myocardial perforations with a central venous catheter are rare in adults (67 cases published sin... more Myocardial perforations with a central venous catheter are rare in adults (67 cases published since 1968). These accidents are fatal in more than two-thirds of the cases owing to confusion caused by misleading symptoms which suggest pulmonary embolism. The perforation is generally localized in the right atrium (29 cases), less frequently in the right ventricle (18 cases). The superior vena cava is rarely affected (3 cases). The site of the perforation was not found in the other published cases. Clinical symptoms are signs of tamponade with disorders of cardiac rhythm. An enlargement of the cardiac shadow and an abnormal position of the catheter, buckled or openly intrapericardial, make the diagnosis radiologically. Echocardiography provides some information, but this is often too late for practical application. The diagnosis is made with right catheterization when it shows an equalization of the diastolic pressures. This allows the patient to be watched closely following the pericardial tap, after which a surgical approach may be indicated and performed. Prevention of these iatrogenic accidents must be systematic and strictly controlled for.
International Journal of Human Resource Management, 2003
What is the reality of contemporary careers? Have individuals become masters of their professiona... more What is the reality of contemporary careers? Have individuals become masters of their professional destiny? While the 'new career literature'puts forward the figure of an individual free to invent his/her career (the 'free actor model'), this article shows that the changes ...
We studied 26 inpatients (17 females; mean age ± SD:41.2 ± l4.3years)who met the DSM III criteria... more We studied 26 inpatients (17 females; mean age ± SD:41.2 ± l4.3years)who met the DSM III criteria for a major depressive episode and had a mean (± SD) Hamilton Depression Score of 19.3 ± 8.0. All patients were drug free and medically healthy at the time of experimentation. We found a significant correlation between the CD4/CD8 ratio and the Hamilton
The present study investigated the effects of long-term estradiol withdrawal (ovariectomy) on hyp... more The present study investigated the effects of long-term estradiol withdrawal (ovariectomy) on hypothalamic serotonin-1A (5-HT(1A)) receptor signaling. Changes in neuroendocrine responses to the 5-HT(1A) agonist 8-OH-DPAT and levels of G(z) protein in the hypothalamus were used to examine 5-HT(1A) receptor signaling. Five days following ovariectomy, rats received daily injections of either 2 microg of beta-estradiol 3-benzoate or vehicle (subcutaneously) for 2, 4 or 14 days. Twenty-four hours after the last injection, and 15 min prior to sacrifice, rats were injected with (+/-)8-OH-DPAT (50 micro;g/kg, s.c.) or saline. Estradiol treatment did not alter basal corticotropin (ACTH) or oxytocin levels. Injection of (+/-)8-OH-DPAT produced significant increases in plasma ACTH and oxytocin levels. In the vehicle-treated rats, hormone responses to 8-OH-DPAT were enhanced in rats that received injections for 14 days compared with rats that received injections for either 2 or 4 days. Estradiol treatment for 4 or 14 days blunted this enhanced ACTH response to 8-OH-DPAT, whereas the oxytocin response to 8-OH-DPAT was only blunted after 14 daily injections of beta-estradiol 3-benzoate. The treatment with beta-estradiol 3-benzoate (2 microg/rat) did not reduce membrane-associated G(z) protein levels in the paraventricular nucleus of the hypothalamus. Hence, the inhibitory influence of a low dose of beta-estradiol 3-benzoate on 5-HT(1A) receptor signaling in the hypothalamus is not accompanied by a change in the levels of G(z) protein in the paraventricular hypothalamic nucleus. Results from the present study indicate a supersensitivity of 5-HT(1A) receptors after withdrawal of estradiol and suggest that estradiol suppresses 5-HT(1A) receptor signaling.
A theoretical model is developed which allows for well-defined lenses to be formed with different... more A theoretical model is developed which allows for well-defined lenses to be formed with different dioptric properties using lasers that are known to ablate a variety of materials. With the theoretical development as a guide an experimental arrangement is defined for the ArF excimer laser and this arrangement is used to form lenses with highly smooth surfaces in three polymeric materials (polymethyl methacrylate, polycarbonate, and polyvinyl chloride) to test the validity of the theory. As part of the procedure to obtain these lenses, ablation curves (etch depth per pulse vs log of the laser fluence) were measured for each of these polymers and these curves are also reported. The experiments were extended to the rabbit cornea in which the corneal button was reshaped as part of keratomileusis surgery. This preliminary experiment resulted in a clear cornea with a smooth corneal surface and a measured change of 3 diopters in the treated eye.
Biochemistry and Cell Biology-biochimie Et Biologie Cellulaire, 2006
The oxidation theory proposes that LDL oxidation is an early event in atherosclerosis and that ox... more The oxidation theory proposes that LDL oxidation is an early event in atherosclerosis and that oxidized LDL contributes to atherogenesis in triggering inflammation. In contrast to the copper-modified LDL, there are few studies using myeloperoxidase-modified LDL (Mox-LDL) as an inflammation inducer. Our aim is to test whether Mox-LDL could constitute a specific inducer of the inflammatory response. Albumin, which is the most abundant protein in plasma and which is present to an identical concentration of LDL in the intima, was used for comparison. The secretion of IL-8 by endothelial cells (Ea.hy926) and TNF-alpha by monocytes (THP-1) was measured in the cell medium after exposure of these cells to native LDL, native albumin, Mox-LDL, or Mox-albumin. We observed that Mox-LDL induced a 1.5- and 2-fold increase (ANOVA; P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001) in IL-8 production at 100 microg/mL and 200 microg/mL, respectively. The incubation of THP-1 cells with Mox-LDL (100 microg/mL) increased the production of TNF-alpha 2-fold over the control. Native LDL, albumin, and Mox-albumin showed no effect in either cellular types. The myeloperoxidase-modified LDL increase in cytokine release by endothelial and monocyte cells and by firing both local and systemic inflammation could induce atherogenesis and its development.
... THE YIELD POTENTIAL UTILIZATION OF SOME SUNFLOWER HYBRIDS IN DIFFERENT CROPYEARS Maria DAM - ... more ... THE YIELD POTENTIAL UTILIZATION OF SOME SUNFLOWER HYBRIDS IN DIFFERENT CROPYEARS Maria DAM - Peter PEPÒ University of Debrecen ... a higher Rubisco content could be one factor in conferring better acclimation and higher drought tolerance (Pankovic et al. ...
ABSTRACT— Hepatitis C virus (HCV) has been proposed to be a cofactor in the pathogenesis of cirrh... more ABSTRACT— Hepatitis C virus (HCV) has been proposed to be a cofactor in the pathogenesis of cirrhosis in patients with chronic alcoholism. The demonstration of a different liver histological pattern in anti-HCV positive patients might provide additional evidence. We studied 164 patients with chronic alcoholism, and histologically proven cirrhosis. For all of them, serum samples were collected at the time of a liver biopsy and stored at –80°C. Testing for anti-HCV antibodies was done using the Ortho Diagnostic Systems Anti-HCV ELISA test. Only reproducible results were considered positive. A semi-quantitative assessment of seven histological parameters was made independently on liver biopsy samples. In the study group, 29 patients (18%) had anti-HCV antibodies. When compared with anti-HCV negative patients, both groups had similar ALT and AST seric activities. Anti-HCV positive patients had a greater score of mononuclear cells infiltrate (0.71±0.57 vs 0.41 ±0.52; p<0.05) and a lesser score of alcoholic hepatitis (0.19 ±0.57 vs 0.74 ±0.74; p<0.005). The scores for steatosis, perisinusoidal and perinodular fibrosis, and hepatocellular necrosis were similar in the two groups. In anti-HCV positive patients, with a clearly positive recombinant immunobinding assay (RIBA, Chiron-Ortho Diagnostic Systems), a greater score for hepatic necrosis and a lesser one for fibrosis were demonstrated. Among the seven patients with active cirrhosis, six were anti-HCV positive. Therefore, HCV is likely to play a role in the pathogenesis of liver damage in a few patients with alcoholic cirrhosis, especially, those with active cirrhosis.
Canadian Journal of Anaesthesia-journal Canadien D Anesthesie, 2007
Objective A left-sided double lumen tube is recommended for one-lung ventilation for most thoraci... more Objective A left-sided double lumen tube is recommended for one-lung ventilation for most thoracic surgeries, but for certain indications, a right-sided double lumen tube (R-DLT) may be mandatory. Frequent malposition of R-DLTs has been reported. We propose an innovative modification of Mallinckrodt’s Broncho-Cath™ R-DLT consisting of an enlarged area of the lateral orifice, and studied the impact of this modification on the adequacy of R-DLT positioning. Methods Eighty adult patients scheduled for elective thoracic surgery were randomized into two groups: standard Broncho-Cath™ R-DLT, or modified Broncho-Cath™ R-DLT. After induction of anesthesia, the R-DLT was positioned using a fibreoptic bronchoscope. The position of the R-DLT was assessed on three occasions: with the patient supine (T1), then immediately following the patient’s transfer to the lateral position (T2), and after repositioning of the tube, when needed, with the patient in lateral position (T3). A score ranging from 1 to 4 was accorded to the relative position of the right upper lobe (RUL) orifice in relation to the origin of the RUL bronchus. Results The modified Broncho-Cath™ R-DLT was more frequently in an adequate position at T2: 77% vs 37% of patients (P = 0.0121), and easier to reposition at T3: 97% vs 74% of patients (P = 0.0109) in comparison to the standard Broncho-Cath™ R-DLT group. Conclusion These data suggest the superiority of the modified Broncho-Cath™ R-DLT compared to a standard Broncho-Cath™ R-DLT for optimal R-DLT positioning to facilitate one-lung ventilation during thoracic surgery. Objectif Une sonde double-lumière gauche est recommandée lors de la ventilation pulmonaire sélective pour la majorité des chirurgies thoraciques; toutefois, dans certaines indications, une sonde double-lumière droite (SDL-D) peut être requise. Il est fait état de la malposition fréquente des SDL-D. Nous proposons une modification de la SDL-D Broncho-Cath™ de Mallinckrodt qui consiste en une surface élargie de l’orifice latéral, et avons étudié l’impact de cette modification sur le bon positionnement de la SDL-D. Méthodes Quatre-vingts patients adultes devant subir une chirurgie thoracique réglée ont été randomisés en deux groupes : SDL-D Broncho-Cath™ standard, ou SDL-D Broncho-Cath™ modifiée. Après induction de l’anesthésie, la SDL-D a été positionnée à l’aide d’un bronchoscope à fibres optiques. La position de la SDL-D a été évaluée trois fois : le patient sur le dos (T1), puis immédiatement après le transfert du patient en position latérale (T2), et après le repositionnement du tube, lorsque requis, le patient toujours en position latérale (T3). Une note de 1 à 4 a été donnée à la position relative de l’orifice du lobe supérieur droit (LSD) par rapport à l’origine de la bronche lobaire supérieure droite. Résultats La SDL-D Broncho-Cath™ modifiée fut plus fréquemment dans une position adéquate en T2: 77 % vs 37 % des patients (P = 0,0121) et fut plus facile à repositionner en T3 : 97 %vs74% des patients (P = 0,0109) par rapport au groupe SDL-D Broncho-Cath™ standard. Conclusion Ces données suggèrent la supériorité de la SDL-D Broncho-Cath™ modifiée par rapport à la SDL-D Broncho-Cath™ standard pour un positionnement optimal de la SDL-D afin de faciliter la ventilation pulmonaire sélective pendant la chirurgie thoracique.
Examines the French and UK traditions of management training and education, and how these have sh... more Examines the French and UK traditions of management training and education, and how these have shaped organizational approaches to human resource development. Presents results from a Price Waterhouse Cranfield survey which shows data based on human resource issues ...
Both ageing and vitamin C (VC) deficiency result in immune defect. Since low serum and tissue lev... more Both ageing and vitamin C (VC) deficiency result in immune defect. Since low serum and tissue levels of VC are found in the elderly, we have in a placebo-controlled study, tested the effect of VC supplements (500 mg/day i.m. for 1 month) on various immune parameters. Indeed, VC enhances the proliferative response of T lymphocytes in vitro, and the tuberculin skin hypersensitivity in vivo. Neither the serum concentrations of IgA, IgG and IgM, nor the proportion of E-rosette-forming cells were modified. No significant change was observed in the placebo-treated group.
Myocardial perforations with a central venous catheter are rare in adults (67 cases published sin... more Myocardial perforations with a central venous catheter are rare in adults (67 cases published since 1968). These accidents are fatal in more than two-thirds of the cases owing to confusion caused by misleading symptoms which suggest pulmonary embolism. The perforation is generally localized in the right atrium (29 cases), less frequently in the right ventricle (18 cases). The superior vena cava is rarely affected (3 cases). The site of the perforation was not found in the other published cases. Clinical symptoms are signs of tamponade with disorders of cardiac rhythm. An enlargement of the cardiac shadow and an abnormal position of the catheter, buckled or openly intrapericardial, make the diagnosis radiologically. Echocardiography provides some information, but this is often too late for practical application. The diagnosis is made with right catheterization when it shows an equalization of the diastolic pressures. This allows the patient to be watched closely following the pericardial tap, after which a surgical approach may be indicated and performed. Prevention of these iatrogenic accidents must be systematic and strictly controlled for.
International Journal of Human Resource Management, 2003
What is the reality of contemporary careers? Have individuals become masters of their professiona... more What is the reality of contemporary careers? Have individuals become masters of their professional destiny? While the 'new career literature'puts forward the figure of an individual free to invent his/her career (the 'free actor model'), this article shows that the changes ...
We studied 26 inpatients (17 females; mean age ± SD:41.2 ± l4.3years)who met the DSM III criteria... more We studied 26 inpatients (17 females; mean age ± SD:41.2 ± l4.3years)who met the DSM III criteria for a major depressive episode and had a mean (± SD) Hamilton Depression Score of 19.3 ± 8.0. All patients were drug free and medically healthy at the time of experimentation. We found a significant correlation between the CD4/CD8 ratio and the Hamilton
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