Vochysia divergens Pohl (Vochysiaceae) is a tree commonly found in wet soils of ‘Pantanal’ of Mat... more Vochysia divergens Pohl (Vochysiaceae) is a tree commonly found in wet soils of ‘Pantanal’ of Mato Grosso, Brazil, and used in folk medicine against infections and asthma. We have studied different extracts and some isolated compounds from this plant for antibacterial activity. From the extracts of the stem bark β-sitosterol, betulinic acid and sericic acid were isolated. The minimal inhibitory
A acne é uma doença multifatorial, que afeta até 85% da população mundial, que tem o Cutibacteriu... more A acne é uma doença multifatorial, que afeta até 85% da população mundial, que tem o Cutibacterium acne como um dos agentes envolvidos em sua fisiopatologia. Seu tratamento tópico com antibacterianos está associado ao aumento da resistência bacteriana..
Silver has a potent antimicrobial action, but its use is still restricted due to toxicity and agg... more Silver has a potent antimicrobial action, but its use is still restricted due to toxicity and aggregation problems. Impregnation with active carbon (AC) has been suggested as an alternative to solve this problem. In this study, termite feces were used to prepare composites containing silver nanoparticles and AC. Reducing agents and different amounts of H2SO4 and silver were used. Antimicrobial activity was assessed using the minimum inhibitory concentration (MIC) and the kinetics of microbial growth of S. aureus, E. coli and C. albicans. Phytotoxicity and acute toxicity were assessed for Cucumis sativus and Artemia salina. The composites that presented MIC 500 μg/mL for S. aureus and E. coli were AC25Ag (6 μg/mL Ag) and AC25AgB (78 μg/mL Ag), respectively. AC6AgS showed antimicrobial activity in C. albicans with MIC of 500 μg/mL (23 μg/mL Ag). AC25AgB inhibited the growth of S. aureus and E. coli for 48 and 32 h, respectively. At a concentration of 10 g/L, the compounds showed delet...
Varios extracto foliares de Bauhinia splend ens, una planta medicinal utilizada en Brasil como re... more Varios extracto foliares de Bauhinia splend ens, una planta medicinal utilizada en Brasil como remedio contra infecciones, se investigaron como agente antibacteriano con el metodo de difusion y el calulo de la cantidad minima inhibitoria (MIC), contra bacterias patogenas Gram-positivas y Gram-negativas. Los extractos menos polares fueron efectivos contra toa las bacterias ensayada". Los extractos en OM y en EA proujeron zonas de inhibicion de aproximadamente 20 mm (2.5 mg/disco) mientraS que el extracto AQE resulto inactivo. Los valores de MIC contra Salmonella typhimurium fueron 0.7 mg/ml para el extracto en DCM y 0,8 mg/ml para el extracto en EA. Cuando se ensayaron contra staphylococcus aureus, ambos extractos mostraron valores de MIC de 0.4 mg/ml. Estos efectos sustentan el uso popular de B. splendens en la medicina folclorica para el tratamiento de enfermedades infecciosa
Wedelia paludosa (Acmela brasiliensis) (Asteraceae), a traditionally used native Brazilian medici... more Wedelia paludosa (Acmela brasiliensis) (Asteraceae), a traditionally used native Brazilian medicinal plant, showed antifungal activity against dermatophytes in dilution tests. The hexane, dichloromethane and butanol fractions displayed activity against Epidermophyton floccosum, Trichophyton rubrum and Trichophyton mentagrophytes, with minimal inhibitory concentrations between 250 and 1000 microg/mL. Two pure compounds, identified as kaurenoic acid (1) and luteolin (2), also showed activity against these dermatophytes.
Diagnostic Microbiology and Infectious Disease, 2004
Susceptibilities to amphotericin B and fluconazole of 383 Candida species isolated from blood wer... more Susceptibilities to amphotericin B and fluconazole of 383 Candida species isolated from blood were determined. Candida albicans was the most common species (55.6%), followed by Candida parapsilosis (17.5%), Candida tropicalis (16.5%), Candida glabrata (5.2%), Candida ...
Taxifolin (3,5,7,3,4-pentahydroxy flavanone or dihydroquercetin, Tax) was identified as a gastrop... more Taxifolin (3,5,7,3,4-pentahydroxy flavanone or dihydroquercetin, Tax) was identified as a gastroprotective compound and a gastroadhesive formulation was recently developed to prolong its residence time and release in the stomach. So, the gastric healing effectiveness of Tax and gastro-mucoadhesive microparticles containing Tax (MPTax) against the acetic acid induced-gastric ulcer in rats was investigated in this study. Moreover, the interactions between Tax and H+/K+-ATPase were investigated in silico, and its anti- H. pylori activity was determined in vitro. The oral treatment with MPTax (81.37 mg/kg, containing 12.29 % of Tax) twice a day for seven days reduced the ulcer area by 63%, compared to vehicle-treated group (Veh: 91.9 ± 10.3 mm2). Tax (10 mg/kg, p.o) reduced the ulcer by 40% but with a p=0.07 versus Veh group. Histological analysis confirmed these effects. Tax and MPTax increased the gastric mucin amount, reduced the myeloperoxidase activity, and increased the glutathione reduced content at ulcer site. However, only MPTax decreased the lipoperoxide accumulation at ulcer site. Besides, Tax and MPTax normalize the catalase and glutathione S-transferase activity. Tax showed reversible interaction with H+/K+-ATPase in silico and its anti-H. pylori effects was confirmed (MIC = 625 μg/mL). These results suggest that the antiulcer property of Tax involves the strengthening of the gastric protective factors in parallel to its inhibitory interaction with H+/K+-ATPase and H. pylori. Considering that ulcer healing action displayed by Tax was favored by gastroadhesive microparticles, this approach seems to be promising for its oral delivery to treat acid-peptic diseases.
Acne affects most young people and its topical treatment with antibacterials is associated with i... more Acne affects most young people and its topical treatment with antibacterials is associated with increased bacterial resistance to antibiotics and adverse effects. As an alternative, ozone therapy stands out through the application of ozonized oils. The objective of this work was to raise the scientific evidence about the effectiveness in the treatment of acne, in addition to the techniques of characterization and stability of ozonated oils. This is an exploratory, descriptive study with a quantitative approach, based on the analysis of scientific references in a bibliographic review of the expository type, of the last 20 years. Among the selected references, only four manuscripts reporting clinical studies of ozone therapy, with controversial results. Seven articles with the physicochemical characterization of ozonated oils were found. The major part of manuscripts reported the use of sunflower, sesame and olive oil. The more common techniques used to characterize the ozonation proc...
Extratos bruto de lipase (EB) obtidos a partir de farelo de arroz previamente desengordurado, for... more Extratos bruto de lipase (EB) obtidos a partir de farelo de arroz previamente desengordurado, foram extraidos com tres diferentes solucoes: 1(CaCb 10mM), 2 (Sacarose 0,6M, KCI 10mM, MgCb 1mM, Ditiotreitol 2mM) e 3 (Sacarose O,4M, DitiotreitoI2mM). A metodologia de superficie de resposta foi aplicada, sendo os fatores (variaveis) estudados: pH (6,0; 7,0 e 8,0) e tempo de extracao (1h, 2h e 3h). A temperatura de extracao foi fixada em 4°C. Os extratos obtidos apresentaram diferentes graus de atividade de lipase, sendo que o extrato bruto que apresentou a maior atividade especifica de lipase, foi a solucao 1 (13,5mU/mg). Realizou-se a purificacao parcial deste extrato bruto atraves de dois metodos distintos de precipitacao de lipase, resultando em extrato semipurificado com acetona (ESP-acetona) e extrato semi-purificado com sulfato de amonio (ESP-sulfato amonio), que apresentaram atividade especifica de 50,7mU/mg e 63mU/mg, respectivamente. Os extratos semi-purificados foram imobiliza...
Vochysia divergens Pohl (Vochysiaceae) is a tree commonly found in wet soils of ‘Pantanal’ of Mat... more Vochysia divergens Pohl (Vochysiaceae) is a tree commonly found in wet soils of ‘Pantanal’ of Mato Grosso, Brazil, and used in folk medicine against infections and asthma. We have studied different extracts and some isolated compounds from this plant for antibacterial activity. From the extracts of the stem bark β-sitosterol, betulinic acid and sericic acid were isolated. The minimal inhibitory
A acne é uma doença multifatorial, que afeta até 85% da população mundial, que tem o Cutibacteriu... more A acne é uma doença multifatorial, que afeta até 85% da população mundial, que tem o Cutibacterium acne como um dos agentes envolvidos em sua fisiopatologia. Seu tratamento tópico com antibacterianos está associado ao aumento da resistência bacteriana..
Silver has a potent antimicrobial action, but its use is still restricted due to toxicity and agg... more Silver has a potent antimicrobial action, but its use is still restricted due to toxicity and aggregation problems. Impregnation with active carbon (AC) has been suggested as an alternative to solve this problem. In this study, termite feces were used to prepare composites containing silver nanoparticles and AC. Reducing agents and different amounts of H2SO4 and silver were used. Antimicrobial activity was assessed using the minimum inhibitory concentration (MIC) and the kinetics of microbial growth of S. aureus, E. coli and C. albicans. Phytotoxicity and acute toxicity were assessed for Cucumis sativus and Artemia salina. The composites that presented MIC 500 μg/mL for S. aureus and E. coli were AC25Ag (6 μg/mL Ag) and AC25AgB (78 μg/mL Ag), respectively. AC6AgS showed antimicrobial activity in C. albicans with MIC of 500 μg/mL (23 μg/mL Ag). AC25AgB inhibited the growth of S. aureus and E. coli for 48 and 32 h, respectively. At a concentration of 10 g/L, the compounds showed delet...
Varios extracto foliares de Bauhinia splend ens, una planta medicinal utilizada en Brasil como re... more Varios extracto foliares de Bauhinia splend ens, una planta medicinal utilizada en Brasil como remedio contra infecciones, se investigaron como agente antibacteriano con el metodo de difusion y el calulo de la cantidad minima inhibitoria (MIC), contra bacterias patogenas Gram-positivas y Gram-negativas. Los extractos menos polares fueron efectivos contra toa las bacterias ensayada". Los extractos en OM y en EA proujeron zonas de inhibicion de aproximadamente 20 mm (2.5 mg/disco) mientraS que el extracto AQE resulto inactivo. Los valores de MIC contra Salmonella typhimurium fueron 0.7 mg/ml para el extracto en DCM y 0,8 mg/ml para el extracto en EA. Cuando se ensayaron contra staphylococcus aureus, ambos extractos mostraron valores de MIC de 0.4 mg/ml. Estos efectos sustentan el uso popular de B. splendens en la medicina folclorica para el tratamiento de enfermedades infecciosa
Wedelia paludosa (Acmela brasiliensis) (Asteraceae), a traditionally used native Brazilian medici... more Wedelia paludosa (Acmela brasiliensis) (Asteraceae), a traditionally used native Brazilian medicinal plant, showed antifungal activity against dermatophytes in dilution tests. The hexane, dichloromethane and butanol fractions displayed activity against Epidermophyton floccosum, Trichophyton rubrum and Trichophyton mentagrophytes, with minimal inhibitory concentrations between 250 and 1000 microg/mL. Two pure compounds, identified as kaurenoic acid (1) and luteolin (2), also showed activity against these dermatophytes.
Diagnostic Microbiology and Infectious Disease, 2004
Susceptibilities to amphotericin B and fluconazole of 383 Candida species isolated from blood wer... more Susceptibilities to amphotericin B and fluconazole of 383 Candida species isolated from blood were determined. Candida albicans was the most common species (55.6%), followed by Candida parapsilosis (17.5%), Candida tropicalis (16.5%), Candida glabrata (5.2%), Candida ...
Taxifolin (3,5,7,3,4-pentahydroxy flavanone or dihydroquercetin, Tax) was identified as a gastrop... more Taxifolin (3,5,7,3,4-pentahydroxy flavanone or dihydroquercetin, Tax) was identified as a gastroprotective compound and a gastroadhesive formulation was recently developed to prolong its residence time and release in the stomach. So, the gastric healing effectiveness of Tax and gastro-mucoadhesive microparticles containing Tax (MPTax) against the acetic acid induced-gastric ulcer in rats was investigated in this study. Moreover, the interactions between Tax and H+/K+-ATPase were investigated in silico, and its anti- H. pylori activity was determined in vitro. The oral treatment with MPTax (81.37 mg/kg, containing 12.29 % of Tax) twice a day for seven days reduced the ulcer area by 63%, compared to vehicle-treated group (Veh: 91.9 ± 10.3 mm2). Tax (10 mg/kg, p.o) reduced the ulcer by 40% but with a p=0.07 versus Veh group. Histological analysis confirmed these effects. Tax and MPTax increased the gastric mucin amount, reduced the myeloperoxidase activity, and increased the glutathione reduced content at ulcer site. However, only MPTax decreased the lipoperoxide accumulation at ulcer site. Besides, Tax and MPTax normalize the catalase and glutathione S-transferase activity. Tax showed reversible interaction with H+/K+-ATPase in silico and its anti-H. pylori effects was confirmed (MIC = 625 μg/mL). These results suggest that the antiulcer property of Tax involves the strengthening of the gastric protective factors in parallel to its inhibitory interaction with H+/K+-ATPase and H. pylori. Considering that ulcer healing action displayed by Tax was favored by gastroadhesive microparticles, this approach seems to be promising for its oral delivery to treat acid-peptic diseases.
Acne affects most young people and its topical treatment with antibacterials is associated with i... more Acne affects most young people and its topical treatment with antibacterials is associated with increased bacterial resistance to antibiotics and adverse effects. As an alternative, ozone therapy stands out through the application of ozonized oils. The objective of this work was to raise the scientific evidence about the effectiveness in the treatment of acne, in addition to the techniques of characterization and stability of ozonated oils. This is an exploratory, descriptive study with a quantitative approach, based on the analysis of scientific references in a bibliographic review of the expository type, of the last 20 years. Among the selected references, only four manuscripts reporting clinical studies of ozone therapy, with controversial results. Seven articles with the physicochemical characterization of ozonated oils were found. The major part of manuscripts reported the use of sunflower, sesame and olive oil. The more common techniques used to characterize the ozonation proc...
Extratos bruto de lipase (EB) obtidos a partir de farelo de arroz previamente desengordurado, for... more Extratos bruto de lipase (EB) obtidos a partir de farelo de arroz previamente desengordurado, foram extraidos com tres diferentes solucoes: 1(CaCb 10mM), 2 (Sacarose 0,6M, KCI 10mM, MgCb 1mM, Ditiotreitol 2mM) e 3 (Sacarose O,4M, DitiotreitoI2mM). A metodologia de superficie de resposta foi aplicada, sendo os fatores (variaveis) estudados: pH (6,0; 7,0 e 8,0) e tempo de extracao (1h, 2h e 3h). A temperatura de extracao foi fixada em 4°C. Os extratos obtidos apresentaram diferentes graus de atividade de lipase, sendo que o extrato bruto que apresentou a maior atividade especifica de lipase, foi a solucao 1 (13,5mU/mg). Realizou-se a purificacao parcial deste extrato bruto atraves de dois metodos distintos de precipitacao de lipase, resultando em extrato semipurificado com acetona (ESP-acetona) e extrato semi-purificado com sulfato de amonio (ESP-sulfato amonio), que apresentaram atividade especifica de 50,7mU/mg e 63mU/mg, respectivamente. Os extratos semi-purificados foram imobiliza...
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