The clinical presentation of COVID-19 and the specific antibody responses associated with SARS-Co... more The clinical presentation of COVID-19 and the specific antibody responses associated with SARS-CoV-2 variants have not been investigated during the emergence of Omicron variants in Bangladesh. The Delta and Omicron variants were identified by post-PCR melting curve analysis of the spike (S) protein receptor binding domain amplicons. Anti-S-protein immunoglobulin-G anti-nucleocapsid (N)-protein immunoglobulin-G and immunoglobulin-A levels were measured by ELISA. The Delta variant was found in 40 out of 40 (100%) SARS-CoV-2 RT-PCR positive COVID-19 patients between 13 September and 23 October 2021 and Omicron variants in 90 out of 90 (100%) RT-PCR positive COVID-19 patients between 9 January and 10 February 2022. The Delta variant associated with hospitalization (74%, 80%, and 40%) and oxygen support (60%, 57%, and 40%) in the no vaccine, dose-1, and dose-2 vaccinated cases, respectively, whereas the Omicron COVID-19 required neither hospitalization nor oxygen support (0%, p < 0.00...
Background The safety of health care workers (HCWs) in Bangladesh and the factors associated with... more Background The safety of health care workers (HCWs) in Bangladesh and the factors associated with getting COVID-19 have been infrequently studied. The aim of this study was to address this gap by assessing the capacity development and safety measures of HCWs in Bangladesh who have been exposed to COVID-19 and by identifying the factors associated with respondents’ self-reported participation in capacity development trainings and their safety practices. Methods This cross-sectional study was based on an online survey of 811 HCWs working at 39 dedicated COVID-19 hospitals in Bangladesh. A pretested structured questionnaire consisting of questions related to respondents’ characteristics, capacity development trainings and safety measures was administered. Binary logistic regressions were run to assess the association between explanatory and dependent variables. Results Among the respondents, 58.1% had been engaged for at least 2 months in COVID-19 care, with 56.5% of them attending cap...
The goal of the study was to develop a specific, sensitive, and cost-effective molecular RT-PCR d... more The goal of the study was to develop a specific, sensitive, and cost-effective molecular RT-PCR diagnostic assay for the rapid and simultaneous detection of the serotypes of dengue virus (DENV) and Chikungunya virus (CHIKV) from sera of suspected febrile patients. A single-tube, single-step multiplex RT-PCR (mRT-PCR) assay was designed for the detection of viral genomes from clinical and field samples. Specificity and sensitivity of the mRT-PCR assay were evaluated against six different combinations using two reverse transcriptases (AMV-RT and RT-Ace) and three DNA polymerases (LA-Taq, rTaq, and Tth). Among the six combinations, the AMV-RT and LA-Taq combination was more specific and sensitive than other enzyme combinations for detecting viral genomes of DENV-1, DENV-2, DENV-3, and DENV-4 (p < 0.01), and for detecting viral genomes of CHIKV (p < 0.05). The detection limits of the mRT-PCR were 10 focus forming units (FFU) for CHIKV and 1 FFU, 20 FFU, 0.1 FFU, and 10 FFU for DEN...
The presentation of Rheumatoid arthritis (RA) combined with a second rheumatological disorder tha... more The presentation of Rheumatoid arthritis (RA) combined with a second rheumatological disorder thatis, different RA overlap syndromes are frequently encountered in clinical practice. But RA-vasculitisoverlaps are relatively rare. This paper presents a case of Rheumatoid arthritis and Granulomatosiswith polyangiitis (Wegener granulomatosis) overlap syndrome which is first of its kind reported from Bangladesh. Bangladesh J Medicine July 2020; 31(2) : 114-119
The current novel coronavirus (nCoV) outbreak, COVID-19, was first reported in December 2019 in W... more The current novel coronavirus (nCoV) outbreak, COVID-19, was first reported in December 2019 in Wuhan, China has spread all over the world causing startling loss of lives, stalling the global economy and disrupting the social life. One of the challenges to contain the COVID-19 is making people adopt personal hygiene, social distancing and self-quarantine practices which are all related to knowledge, attitude and practice (KAP) of the people in respective countries. Bangladesh, the most densely populated countries with a fast-growing economy and moderate literacy rate, has shown many hiccups in its efforts to implement COVID-19 policies. Understanding KAP may give the policy makers to make informed decisions. Hence, this study aimed to make a quick assessment of KAP of people in relation to COVID-19 in Bangladesh. An online survey using a pre-tested questionnaire conducted in late March 2020 attained 1837 responses across Bangladesh. However, 1589 completed responses were included in...
Hailey-Hailey disease (HHD) is an autosomal-dominant genodermatosis, associated with abnormal epi... more Hailey-Hailey disease (HHD) is an autosomal-dominant genodermatosis, associated with abnormal epithelial cell adhesion due to altered calcium metabolism. Clinical features involve painful red blisters, erosions, fissured and hypertrophic plaques predominantly in the intertriginous areas. Heat, friction, minor trauma, superimposed bacterial or viral infection can aggravate the condition. Here, we report a case of a 50-year old lady with no previous family history presented with severe attack of HHD since last 5 months. Histology showed acantholysis of keratinocytes resembling characteristic dilapidated brick wall appearance in the epidermis layer of skin which helped for the confirmed diagnosis. Bangladesh J Medicine Jan 2020; 31(1) : 29-32
Background: Polyneuropathy has many different causes. It is often very difficult to find out the ... more Background: Polyneuropathy has many different causes. It is often very difficult to find out the cause. Nerve conduction study (NCS) can classify neuropathy as axonal and demyelinatig variety and direct the search for cause. Methodology: Purposively selected 80 patients from the department of Neurology Dhaka Medical College during the period of January 2009 to June 2010 were taken for NCS whose were compatible with polyneuropathy by history and clinical examination. Clinical, electrophysiological feature and pattern of polyneuropathy were analyzed. Results: Mean age of the patients was 34.5 ±6.8 and M: F was 1.8:1. Students, laborer and cultivators were the most affected people. 55% patients were acute cases and 35% patients were chronic Cases. 30% patient had no known risk factor for neuropathy 25% patient had antecedent infection, 15% had diabetes mellitus, 7.5% were exposed to drugs/toxins or solvents and 5% had family history of neuropathy. In clinical examination 37.5% patients...
Background: Magnesium deficiency has been a common, but easily ignored, electrolyte abnormality. ... more Background: Magnesium deficiency has been a common, but easily ignored, electrolyte abnormality. Studies on magnesium in our country are lacking. Here, we have estimated and correlated serum magnesium levels with outcomes and other electrolyte imbalance in critically ill patients with respect to the mortality, in hospital outcome and length of high dependency unit (HDU) stay.Methods: A prospective, observational study was conducted in patients who had been admitted to the HDU. Patient’s demographic profile, medical history, serum magnesium, calcium and electrolytes were enrolled on admission. Patients were divided into normomagnesemic, hypomagnesemic hypermagnesemic groups and compared for various parameters.Results: Out of 60 critically ill-patients, 32 patients (53.33%) were hypomagnesemic, 20 patients (33.33%) were normomagnesemic and 8 patients (13.33%) were hypermagnesemic. The duration of stay of the patients in HDU, in hospital outcome and mortality showed significant variati...
The clinical presentation of COVID-19 and the specific antibody responses associated with SARS-Co... more The clinical presentation of COVID-19 and the specific antibody responses associated with SARS-CoV-2 variants have not been investigated during the emergence of Omicron variants in Bangladesh. The Delta and Omicron variants were identified by post-PCR melting curve analysis of the spike (S) protein receptor binding domain amplicons. Anti-S-protein immunoglobulin-G anti-nucleocapsid (N)-protein immunoglobulin-G and immunoglobulin-A levels were measured by ELISA. The Delta variant was found in 40 out of 40 (100%) SARS-CoV-2 RT-PCR positive COVID-19 patients between 13 September and 23 October 2021 and Omicron variants in 90 out of 90 (100%) RT-PCR positive COVID-19 patients between 9 January and 10 February 2022. The Delta variant associated with hospitalization (74%, 80%, and 40%) and oxygen support (60%, 57%, and 40%) in the no vaccine, dose-1, and dose-2 vaccinated cases, respectively, whereas the Omicron COVID-19 required neither hospitalization nor oxygen support (0%, p < 0.00...
Background The safety of health care workers (HCWs) in Bangladesh and the factors associated with... more Background The safety of health care workers (HCWs) in Bangladesh and the factors associated with getting COVID-19 have been infrequently studied. The aim of this study was to address this gap by assessing the capacity development and safety measures of HCWs in Bangladesh who have been exposed to COVID-19 and by identifying the factors associated with respondents’ self-reported participation in capacity development trainings and their safety practices. Methods This cross-sectional study was based on an online survey of 811 HCWs working at 39 dedicated COVID-19 hospitals in Bangladesh. A pretested structured questionnaire consisting of questions related to respondents’ characteristics, capacity development trainings and safety measures was administered. Binary logistic regressions were run to assess the association between explanatory and dependent variables. Results Among the respondents, 58.1% had been engaged for at least 2 months in COVID-19 care, with 56.5% of them attending cap...
The goal of the study was to develop a specific, sensitive, and cost-effective molecular RT-PCR d... more The goal of the study was to develop a specific, sensitive, and cost-effective molecular RT-PCR diagnostic assay for the rapid and simultaneous detection of the serotypes of dengue virus (DENV) and Chikungunya virus (CHIKV) from sera of suspected febrile patients. A single-tube, single-step multiplex RT-PCR (mRT-PCR) assay was designed for the detection of viral genomes from clinical and field samples. Specificity and sensitivity of the mRT-PCR assay were evaluated against six different combinations using two reverse transcriptases (AMV-RT and RT-Ace) and three DNA polymerases (LA-Taq, rTaq, and Tth). Among the six combinations, the AMV-RT and LA-Taq combination was more specific and sensitive than other enzyme combinations for detecting viral genomes of DENV-1, DENV-2, DENV-3, and DENV-4 (p < 0.01), and for detecting viral genomes of CHIKV (p < 0.05). The detection limits of the mRT-PCR were 10 focus forming units (FFU) for CHIKV and 1 FFU, 20 FFU, 0.1 FFU, and 10 FFU for DEN...
The presentation of Rheumatoid arthritis (RA) combined with a second rheumatological disorder tha... more The presentation of Rheumatoid arthritis (RA) combined with a second rheumatological disorder thatis, different RA overlap syndromes are frequently encountered in clinical practice. But RA-vasculitisoverlaps are relatively rare. This paper presents a case of Rheumatoid arthritis and Granulomatosiswith polyangiitis (Wegener granulomatosis) overlap syndrome which is first of its kind reported from Bangladesh. Bangladesh J Medicine July 2020; 31(2) : 114-119
The current novel coronavirus (nCoV) outbreak, COVID-19, was first reported in December 2019 in W... more The current novel coronavirus (nCoV) outbreak, COVID-19, was first reported in December 2019 in Wuhan, China has spread all over the world causing startling loss of lives, stalling the global economy and disrupting the social life. One of the challenges to contain the COVID-19 is making people adopt personal hygiene, social distancing and self-quarantine practices which are all related to knowledge, attitude and practice (KAP) of the people in respective countries. Bangladesh, the most densely populated countries with a fast-growing economy and moderate literacy rate, has shown many hiccups in its efforts to implement COVID-19 policies. Understanding KAP may give the policy makers to make informed decisions. Hence, this study aimed to make a quick assessment of KAP of people in relation to COVID-19 in Bangladesh. An online survey using a pre-tested questionnaire conducted in late March 2020 attained 1837 responses across Bangladesh. However, 1589 completed responses were included in...
Hailey-Hailey disease (HHD) is an autosomal-dominant genodermatosis, associated with abnormal epi... more Hailey-Hailey disease (HHD) is an autosomal-dominant genodermatosis, associated with abnormal epithelial cell adhesion due to altered calcium metabolism. Clinical features involve painful red blisters, erosions, fissured and hypertrophic plaques predominantly in the intertriginous areas. Heat, friction, minor trauma, superimposed bacterial or viral infection can aggravate the condition. Here, we report a case of a 50-year old lady with no previous family history presented with severe attack of HHD since last 5 months. Histology showed acantholysis of keratinocytes resembling characteristic dilapidated brick wall appearance in the epidermis layer of skin which helped for the confirmed diagnosis. Bangladesh J Medicine Jan 2020; 31(1) : 29-32
Background: Polyneuropathy has many different causes. It is often very difficult to find out the ... more Background: Polyneuropathy has many different causes. It is often very difficult to find out the cause. Nerve conduction study (NCS) can classify neuropathy as axonal and demyelinatig variety and direct the search for cause. Methodology: Purposively selected 80 patients from the department of Neurology Dhaka Medical College during the period of January 2009 to June 2010 were taken for NCS whose were compatible with polyneuropathy by history and clinical examination. Clinical, electrophysiological feature and pattern of polyneuropathy were analyzed. Results: Mean age of the patients was 34.5 ±6.8 and M: F was 1.8:1. Students, laborer and cultivators were the most affected people. 55% patients were acute cases and 35% patients were chronic Cases. 30% patient had no known risk factor for neuropathy 25% patient had antecedent infection, 15% had diabetes mellitus, 7.5% were exposed to drugs/toxins or solvents and 5% had family history of neuropathy. In clinical examination 37.5% patients...
Background: Magnesium deficiency has been a common, but easily ignored, electrolyte abnormality. ... more Background: Magnesium deficiency has been a common, but easily ignored, electrolyte abnormality. Studies on magnesium in our country are lacking. Here, we have estimated and correlated serum magnesium levels with outcomes and other electrolyte imbalance in critically ill patients with respect to the mortality, in hospital outcome and length of high dependency unit (HDU) stay.Methods: A prospective, observational study was conducted in patients who had been admitted to the HDU. Patient’s demographic profile, medical history, serum magnesium, calcium and electrolytes were enrolled on admission. Patients were divided into normomagnesemic, hypomagnesemic hypermagnesemic groups and compared for various parameters.Results: Out of 60 critically ill-patients, 32 patients (53.33%) were hypomagnesemic, 20 patients (33.33%) were normomagnesemic and 8 patients (13.33%) were hypermagnesemic. The duration of stay of the patients in HDU, in hospital outcome and mortality showed significant variati...
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Papers by Professor Md Robed Amin