Vitamin B and its derivatives possess diverse physiological functions and are essential micronutr... more Vitamin B and its derivatives possess diverse physiological functions and are essential micronutrients for humans. Their variation in crops is important for the identification of genetic resources used to develop new varieties with enhanced vitamin B. In this research, remarkable variations were observed in kernels of 156 maize inbred lines, ranging from 107.61 to 2654.54 μg per 100 g for vitamin B1, 1.19–37.37 μg per 100 g for B2, 19.60–213.75 μg per 100 g for B3, 43.47–590.86 μg per 100 g for B5, and 138.59–1065.11 μg per 100 g for B6. Growing inbreeds in Hainan and Hebei provinces of China revealed environmental and genotype interactions among these vitamins and the correlations between them in maize grain. Several inbred lines were identified as good sources of vitamin B and promising germplasms for maize breeding, namely By855 and Si273 are overall rich in all the studied vitamins, and GY386B and CML118 are specially enriched with derivatives of vitamin B6. The present study ca...
ABSTRACT Natural growth substances has effective role in callus culture of Banana (Musa spp.) cul... more ABSTRACT Natural growth substances has effective role in callus culture of Banana (Musa spp.) cultivar 'Anupam' (AAB Genome, Sapientum Subgroup) ABSTRACT This experimental design was conducted to establish the effect of organic nutrients for in vitro morphogenesis on callus of Musa sapientum cv Anupam (Family: Musaceae). This cultivar is a well distributed herbaceous flowering plant in Bangladesh and highly favored by the people for its edible parts (fruit, flower and stem) and serves as a regular nutritional supplement in diet for notably carbohydrate, protein, vitamin B, potassium and calcium. The objective of the study was to develop a suitable protocol for callus culture of this species using organic supplements. In vitro generated calluses of this cultivar which were induced from leaf and stem segments were cultured onto Murashige and Skoog (MS) medium containing 2,4-Dichlorophenoxyacetic acid [2,4-D] (3mg/L) + 1-Naphthaleneacetic acid [NAA] (0.5mg/L), and was used as experimental plant resource. In vitro grown calluses were sub-cultured on MS medium containing different concentrations of coconut water (CW), casein hydrolysate (CH), biotin (Bn), peptone (Pn), yeast extract (YE) and activated charcoal (AC). The pH of the medium was adjusted at 5.8. The highest average callus index number (CIN) (2.65) was recorded in MS + CW (15%) after 28 days. Colors of induced calluses were varied with respect to different additives. In the MS medium containing 15% coconut water, 100% were greenish and fragile and where 60% were non-embryonic and 70% showed root formation. 90% root formation was observed when MS medium was enriched with 20% CW. Addition of CH (200mg/l) and Bn (0.5mg/l) in MS medium was also found to be effective in promoting callus growth and rhizogenesis in calluses. These observations revealed that morphogenic responses could play a role for physiological studies in vitro and served to develop a suitable composition of medium for callus culture for this species from natural and organic sources.
Evidence-Based Complementary and Alternative Medicine, 2014
Plants have served as sources of food and medicines for human beings since their advent. During f... more Plants have served as sources of food and medicines for human beings since their advent. During famines or conditions of food scarcity, people throughout the world depend on unconventional plant items to satiate their hunger and meet their nutritional needs. Malnourished people often suffer from various diseases, much more than people eating a balanced diet. We are hypothesizing that the unconventional food plants that people eat during times of scarcity of their normal diet are also medicinal plants and thus can play a role in satiating hunger, meeting nutritional needs, and serving therapeutic purposes. Towards testing our hypothesis, surveys were carried out among the low income people of four villages in Lalmonirhat and Nilphamari districts of Bangladesh. People and particularly the low income people of these two districts suffer each year from a seasonal famine known as Monga. Over 200 informants from 167 households in the villages were interviewed with the help of a semistruct...
Vitamin B and its derivatives possess diverse physiological functions and are essential micronutr... more Vitamin B and its derivatives possess diverse physiological functions and are essential micronutrients for humans. Their variation in crops is important for the identification of genetic resources used to develop new varieties with enhanced vitamin B. In this research, remarkable variations were observed in kernels of 156 maize inbred lines, ranging from 107.61 to 2654.54 μg per 100 g for vitamin B1, 1.19–37.37 μg per 100 g for B2, 19.60–213.75 μg per 100 g for B3, 43.47–590.86 μg per 100 g for B5, and 138.59–1065.11 μg per 100 g for B6. Growing inbreeds in Hainan and Hebei provinces of China revealed environmental and genotype interactions among these vitamins and the correlations between them in maize grain. Several inbred lines were identified as good sources of vitamin B and promising germplasms for maize breeding, namely By855 and Si273 are overall rich in all the studied vitamins, and GY386B and CML118 are specially enriched with derivatives of vitamin B6. The present study ca...
ABSTRACT Natural growth substances has effective role in callus culture of Banana (Musa spp.) cul... more ABSTRACT Natural growth substances has effective role in callus culture of Banana (Musa spp.) cultivar 'Anupam' (AAB Genome, Sapientum Subgroup) ABSTRACT This experimental design was conducted to establish the effect of organic nutrients for in vitro morphogenesis on callus of Musa sapientum cv Anupam (Family: Musaceae). This cultivar is a well distributed herbaceous flowering plant in Bangladesh and highly favored by the people for its edible parts (fruit, flower and stem) and serves as a regular nutritional supplement in diet for notably carbohydrate, protein, vitamin B, potassium and calcium. The objective of the study was to develop a suitable protocol for callus culture of this species using organic supplements. In vitro generated calluses of this cultivar which were induced from leaf and stem segments were cultured onto Murashige and Skoog (MS) medium containing 2,4-Dichlorophenoxyacetic acid [2,4-D] (3mg/L) + 1-Naphthaleneacetic acid [NAA] (0.5mg/L), and was used as experimental plant resource. In vitro grown calluses were sub-cultured on MS medium containing different concentrations of coconut water (CW), casein hydrolysate (CH), biotin (Bn), peptone (Pn), yeast extract (YE) and activated charcoal (AC). The pH of the medium was adjusted at 5.8. The highest average callus index number (CIN) (2.65) was recorded in MS + CW (15%) after 28 days. Colors of induced calluses were varied with respect to different additives. In the MS medium containing 15% coconut water, 100% were greenish and fragile and where 60% were non-embryonic and 70% showed root formation. 90% root formation was observed when MS medium was enriched with 20% CW. Addition of CH (200mg/l) and Bn (0.5mg/l) in MS medium was also found to be effective in promoting callus growth and rhizogenesis in calluses. These observations revealed that morphogenic responses could play a role for physiological studies in vitro and served to develop a suitable composition of medium for callus culture for this species from natural and organic sources.
Evidence-Based Complementary and Alternative Medicine, 2014
Plants have served as sources of food and medicines for human beings since their advent. During f... more Plants have served as sources of food and medicines for human beings since their advent. During famines or conditions of food scarcity, people throughout the world depend on unconventional plant items to satiate their hunger and meet their nutritional needs. Malnourished people often suffer from various diseases, much more than people eating a balanced diet. We are hypothesizing that the unconventional food plants that people eat during times of scarcity of their normal diet are also medicinal plants and thus can play a role in satiating hunger, meeting nutritional needs, and serving therapeutic purposes. Towards testing our hypothesis, surveys were carried out among the low income people of four villages in Lalmonirhat and Nilphamari districts of Bangladesh. People and particularly the low income people of these two districts suffer each year from a seasonal famine known as Monga. Over 200 informants from 167 households in the villages were interviewed with the help of a semistruct...
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Papers by F. M. Safiul Azam