Dendrobium orchids are economically important as cut flowers. The transformation of Dendrobium us... more Dendrobium orchids are economically important as cut flowers. The transformation of Dendrobium using Agrobacterium tumefaciens has emerged as an important protocol in improving the quality of the orchid. The T-DNA gene transfer conditions have to be optimised to ensure successful Agrobacterium transformation. Agrobacterium chemotaxis and attachment studies were carried out on protocorm-like bodies (PLBs) of Dendrobium sonia-28 to determine the potential strength of the Agrobacterium-PLB interaction during the transformation event. The chemotactic response and attachment of the Agrobacterium to the PLBs were positive. The greatest bacterial movement (ratio between 1.2-1.5) was obtained when 3-4mm PLBs were used for transformation, when compared to 1-2mm PLBs. Higher Agrobacterium attachment rates (40-75%) were obtained in 3-4mm PLBs when compared to 1-2mm PLBs. Protocorm-like body-wounding does not significantly attract the bacteria, but efficiently increased bacterial attachment sit...
Background. Acute myeloid leukemia (AML) persists to be a major health problem especially among c... more Background. Acute myeloid leukemia (AML) persists to be a major health problem especially among children as effective chemotherapy to combat the disease is yet to be available. Boswellia dalzielii is a well-known herb that is traditionally used for treatment and management of many diseases including degenerative diseases. In this study, silver nanoparticles were synthesized from the phytochemicals of B. dalzielii stem bark aqueous extract. The silver nanoparticles were characterized by carrying out Fourier Transform Infrared (FTIR) spectroscopy, Energy Filtered Scanning Electron Microscopy (FESEM), X-ray diffraction, and Dynamic Light Scattering (DLS) analyses. Antioxidant capacity of the nanoparticles was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, and the antiproliferative effect of the nanoparticles on Kasumi-1 leukemia cells was investigated using PrestoBlue assay. Flow cytometry analysis was performed to observe the effect of the nanoparticles on the leukemia ce...
Abstract The anticancer properties of the plant’s parts of Moringa oleifera have been well studie... more Abstract The anticancer properties of the plant’s parts of Moringa oleifera have been well studied and reported in peer-reviewed journals and other publications. Here, we focused on the anticancer effects of Moringa oleifera seeds by discussing the role of its metabolites in activating apoptosis and arrest of cell cycle progression in cancer cells. Many consumers and users of the plant prefer the seeds to be eaten because of its sweet taste and some other people use the seeds for purification of water because of its coagulating power. The seeds are rich in metabolites, which are majorly polyphenols and fatty acids, and some of these metabolites play significant roles in inhibiting cancer cells by inducing apoptosis and arrest of cell cycle progression. Polyphenols (such as ellagic acid, p-coumaric acid, gallic acids, cinnamic acid, caffeic acid, protocatechuic acid, kaempferol, catechin, ferulic acid, and quercetin) and fatty acids (such as stearic acid, oleic acid, palmitic acid, and caprylic acid) were found to activate apoptosis in several cancer cells like lung, leukemia, and ovarian cancer cells. The metabolites that have been reported to induce arrest of cell cycle progression in cancer cells at G1, S, and G2-M phases include ellagic acid, quercetin, caprylic acid, and oleic acid. In general, based on the existing academic reports, the seeds of Moringa oleifera is rich in phytochemical and metabolites that can activate apoptosis and induce arrest of cell cycle in cancer cells. The activities lead to the death of cancer cell; hence the seeds have promising potent anticancer agents that need to be further explored especially in clinical research.
: In order to investigate the structure and electrical conductivity of (DNA), Scanning Tunnelling... more : In order to investigate the structure and electrical conductivity of (DNA), Scanning Tunnelling Microscopy (STM) studies were carried out on a model of DNA which was composed of a 12-mer single-stranded polyadenine connected via sugar-phosphate backbone. The 12-mer single-stranded polyadenine molecules were found to be aligned in parallel to each other at a separation 25±7.5 Å. This alignment formed a one-dimensional chain structure, which indicated that the side-by-side coupling among the molecules was strong. The STM measurements with constant current mode and fixed bias voltage, demonstrate that, the electrical conductivity of DNA bases could be deduced by monitoring the size of the structure of 12-mer single-stranded polyadenine.
This study was conducted to determine the feasibility of using three plastid DNA regions (,-, and... more This study was conducted to determine the feasibility of using three plastid DNA regions (,-, and) as DNA barcodes to identify the medicinal plant. In this study,was collected at several different locations. Total genomic DNA was extracted, amplified by polymerase chain reaction (PCR), and sequenced using,-, and, primers. DNA sequences generated from PCR were submitted to the National Center for Biotechnology Information's (NCBI) GenBank. Identification ofwas carried out using NCBI's Basic Local Alignment Search Tool (BLAST). Theand-regions successfully identifiedwith sequencing rates of 100% through BLAST identification. Molecular Evolutionary Genetics Analysis (MEGA) 6.0 was used to analyze interspecific and intraspecific divergence of plastid DNA sequences.andexhibited the lowest average interspecific distance (0.0487 and 0.0963, respectively), whereas-exhibited the highest average interspecific distance (0.2029). The R package Spider revealed that-correctly identified Ba...
Recombinant protein overexpression, an important biotechnological process, is ruled by complex bi... more Recombinant protein overexpression, an important biotechnological process, is ruled by complex biological rules which are mostly unknown, is in need of an intelligent algorithm so as to avoid resource-intensive lab-based trial and error experiments in order to determine the expression level of the recombinant protein. The purpose of this study is to propose a predictive model to estimate the level of recombinant protein overexpression for the first time in the literature using a machine learning approach based on the sequence, expression vector, and expression host. The expression host was confined to Escherichia coli which is the most popular bacterial host to overexpress recombinant proteins. To provide a handle to the problem, the overexpression level was categorized as low, medium and high. A set of features which were likely to affect the overexpression level was generated based on the known facts (e.g. gene length) and knowledge gathered from related literature. Then, a representative sub-set of features generated in the previous objective was determined using feature selection techniques. Finally a predictive model was developed using random forest classifier which was able to adequately classify the multi-class imbalanced small dataset constructed. The result showed that the predictive model provided a promising accuracy of 80% on average, in estimating the overexpression level of a recombinant protein.
This study was designed to investigate the surface properties especially surface porosity of poly... more This study was designed to investigate the surface properties especially surface porosity of polyhydroxybutyrate (PHB) using scanning electron microscopy. PHB granules were sprinkled on the double-sided sticky tape attached on a SEM aluminium stub and sputtered with gold(10nm thickness) in a Polaron SC515 Coater, following which the samples were placed into the SEM specimen chamber for viewing and recording. Scanning electron micrographs with different magnification of PHB surface revealed multiple pores with different sizes.
In this post genomic era, there are a great number of in silico annotated hypothetical genes. How... more In this post genomic era, there are a great number of in silico annotated hypothetical genes. However, experimental validation of the functionality of these genes remains tentative. Two of the major challenges faced by researcher are whether these hypothetical genes are protein-coding genes and whether their corresponding predicted translational start codons are correct. In this report, we demonstrate a convenient procedure to validate the presence of a hypothetical gene product of BPSS1356 from Burkholderia pseudomallei as well as its start codon. It was done by integration of a His-Tag coding sequence into C-terminal end of BPSS1356 gene via homologous recombination. We then purified the native protein using affinity chromatography. The genuine start codon of BPSS1356 was then determined by protein N-terminal sequencing.
Abstract: Polyhydroxybutyrate (PHB) is a polyhydroxyalkanoate (PHA), a polymer belonging to polye... more Abstract: Polyhydroxybutyrate (PHB) is a polyhydroxyalkanoate (PHA), a polymer belonging to polyesters class and is composed of hydroxy fatty acids. PHB is produced by microorganisms apparently in response to conditions of physiological stress. PHB ...
Dendrobium orchids are economically important as cut flowers. The transformation of Dendrobium us... more Dendrobium orchids are economically important as cut flowers. The transformation of Dendrobium using Agrobacterium tumefaciens has emerged as an important protocol in improving the quality of the orchid. The T-DNA gene transfer conditions have to be optimised to ensure successful Agrobacterium transformation. Agrobacterium chemotaxis and attachment studies were carried out on protocorm-like bodies (PLBs) of Dendrobium sonia-28 to determine the potential strength of the Agrobacterium-PLB interaction during the transformation event. The chemotactic response and attachment of the Agrobacterium to the PLBs were positive. The greatest bacterial movement (ratio between 1.2-1.5) was obtained when 3-4mm PLBs were used for transformation, when compared to 1-2mm PLBs. Higher Agrobacterium attachment rates (40-75%) were obtained in 3-4mm PLBs when compared to 1-2mm PLBs. Protocorm-like body-wounding does not significantly attract the bacteria, but efficiently increased bacterial attachment sit...
Background. Acute myeloid leukemia (AML) persists to be a major health problem especially among c... more Background. Acute myeloid leukemia (AML) persists to be a major health problem especially among children as effective chemotherapy to combat the disease is yet to be available. Boswellia dalzielii is a well-known herb that is traditionally used for treatment and management of many diseases including degenerative diseases. In this study, silver nanoparticles were synthesized from the phytochemicals of B. dalzielii stem bark aqueous extract. The silver nanoparticles were characterized by carrying out Fourier Transform Infrared (FTIR) spectroscopy, Energy Filtered Scanning Electron Microscopy (FESEM), X-ray diffraction, and Dynamic Light Scattering (DLS) analyses. Antioxidant capacity of the nanoparticles was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, and the antiproliferative effect of the nanoparticles on Kasumi-1 leukemia cells was investigated using PrestoBlue assay. Flow cytometry analysis was performed to observe the effect of the nanoparticles on the leukemia ce...
Abstract The anticancer properties of the plant’s parts of Moringa oleifera have been well studie... more Abstract The anticancer properties of the plant’s parts of Moringa oleifera have been well studied and reported in peer-reviewed journals and other publications. Here, we focused on the anticancer effects of Moringa oleifera seeds by discussing the role of its metabolites in activating apoptosis and arrest of cell cycle progression in cancer cells. Many consumers and users of the plant prefer the seeds to be eaten because of its sweet taste and some other people use the seeds for purification of water because of its coagulating power. The seeds are rich in metabolites, which are majorly polyphenols and fatty acids, and some of these metabolites play significant roles in inhibiting cancer cells by inducing apoptosis and arrest of cell cycle progression. Polyphenols (such as ellagic acid, p-coumaric acid, gallic acids, cinnamic acid, caffeic acid, protocatechuic acid, kaempferol, catechin, ferulic acid, and quercetin) and fatty acids (such as stearic acid, oleic acid, palmitic acid, and caprylic acid) were found to activate apoptosis in several cancer cells like lung, leukemia, and ovarian cancer cells. The metabolites that have been reported to induce arrest of cell cycle progression in cancer cells at G1, S, and G2-M phases include ellagic acid, quercetin, caprylic acid, and oleic acid. In general, based on the existing academic reports, the seeds of Moringa oleifera is rich in phytochemical and metabolites that can activate apoptosis and induce arrest of cell cycle in cancer cells. The activities lead to the death of cancer cell; hence the seeds have promising potent anticancer agents that need to be further explored especially in clinical research.
: In order to investigate the structure and electrical conductivity of (DNA), Scanning Tunnelling... more : In order to investigate the structure and electrical conductivity of (DNA), Scanning Tunnelling Microscopy (STM) studies were carried out on a model of DNA which was composed of a 12-mer single-stranded polyadenine connected via sugar-phosphate backbone. The 12-mer single-stranded polyadenine molecules were found to be aligned in parallel to each other at a separation 25±7.5 Å. This alignment formed a one-dimensional chain structure, which indicated that the side-by-side coupling among the molecules was strong. The STM measurements with constant current mode and fixed bias voltage, demonstrate that, the electrical conductivity of DNA bases could be deduced by monitoring the size of the structure of 12-mer single-stranded polyadenine.
This study was conducted to determine the feasibility of using three plastid DNA regions (,-, and... more This study was conducted to determine the feasibility of using three plastid DNA regions (,-, and) as DNA barcodes to identify the medicinal plant. In this study,was collected at several different locations. Total genomic DNA was extracted, amplified by polymerase chain reaction (PCR), and sequenced using,-, and, primers. DNA sequences generated from PCR were submitted to the National Center for Biotechnology Information's (NCBI) GenBank. Identification ofwas carried out using NCBI's Basic Local Alignment Search Tool (BLAST). Theand-regions successfully identifiedwith sequencing rates of 100% through BLAST identification. Molecular Evolutionary Genetics Analysis (MEGA) 6.0 was used to analyze interspecific and intraspecific divergence of plastid DNA sequences.andexhibited the lowest average interspecific distance (0.0487 and 0.0963, respectively), whereas-exhibited the highest average interspecific distance (0.2029). The R package Spider revealed that-correctly identified Ba...
Recombinant protein overexpression, an important biotechnological process, is ruled by complex bi... more Recombinant protein overexpression, an important biotechnological process, is ruled by complex biological rules which are mostly unknown, is in need of an intelligent algorithm so as to avoid resource-intensive lab-based trial and error experiments in order to determine the expression level of the recombinant protein. The purpose of this study is to propose a predictive model to estimate the level of recombinant protein overexpression for the first time in the literature using a machine learning approach based on the sequence, expression vector, and expression host. The expression host was confined to Escherichia coli which is the most popular bacterial host to overexpress recombinant proteins. To provide a handle to the problem, the overexpression level was categorized as low, medium and high. A set of features which were likely to affect the overexpression level was generated based on the known facts (e.g. gene length) and knowledge gathered from related literature. Then, a representative sub-set of features generated in the previous objective was determined using feature selection techniques. Finally a predictive model was developed using random forest classifier which was able to adequately classify the multi-class imbalanced small dataset constructed. The result showed that the predictive model provided a promising accuracy of 80% on average, in estimating the overexpression level of a recombinant protein.
This study was designed to investigate the surface properties especially surface porosity of poly... more This study was designed to investigate the surface properties especially surface porosity of polyhydroxybutyrate (PHB) using scanning electron microscopy. PHB granules were sprinkled on the double-sided sticky tape attached on a SEM aluminium stub and sputtered with gold(10nm thickness) in a Polaron SC515 Coater, following which the samples were placed into the SEM specimen chamber for viewing and recording. Scanning electron micrographs with different magnification of PHB surface revealed multiple pores with different sizes.
In this post genomic era, there are a great number of in silico annotated hypothetical genes. How... more In this post genomic era, there are a great number of in silico annotated hypothetical genes. However, experimental validation of the functionality of these genes remains tentative. Two of the major challenges faced by researcher are whether these hypothetical genes are protein-coding genes and whether their corresponding predicted translational start codons are correct. In this report, we demonstrate a convenient procedure to validate the presence of a hypothetical gene product of BPSS1356 from Burkholderia pseudomallei as well as its start codon. It was done by integration of a His-Tag coding sequence into C-terminal end of BPSS1356 gene via homologous recombination. We then purified the native protein using affinity chromatography. The genuine start codon of BPSS1356 was then determined by protein N-terminal sequencing.
Abstract: Polyhydroxybutyrate (PHB) is a polyhydroxyalkanoate (PHA), a polymer belonging to polye... more Abstract: Polyhydroxybutyrate (PHB) is a polyhydroxyalkanoate (PHA), a polymer belonging to polyesters class and is composed of hydroxy fatty acids. PHB is produced by microorganisms apparently in response to conditions of physiological stress. PHB ...
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