Using a cDNA probe, two genomic clones were obtained encoding the human tissue inhibitor of metal... more Using a cDNA probe, two genomic clones were obtained encoding the human tissue inhibitor of metalloproteinases-3 (TIMP-3). Analysis of these clones showed that they contained four distal exons and three introns of the gene. Although the intron-exon structure is similar to that of the timp1 gene, the first intron of the timp3 gene is much longer, being at least 17.5 kb in size.
Biocatalysis and agricultural biotechnology, Sep 1, 2020
Abstract This study aims to determine the phytochemical compounds of Cistus ladaniferus subsp. la... more Abstract This study aims to determine the phytochemical compounds of Cistus ladaniferus subsp. ladanifer and Mentha suaveolens and the investigations of their antibacterial effects against phytopathogenic bacteria. The chemical composition of Cistus ladaniferus subsp. ladanifer essential oils (CLEO) and Mentha suaveolens essential oils (MSEO) was determined using GC-MS analysis. The determination of phenolic content (TPC) of ethanolic, methanolic, and water plant extracts was realized by the Folin-Ciocalteu method. In vitro antibacterial activity was tested against Pseudomonas savastanoi pv. savastanoi 2636-40 and Clavibacter michiganensis subsp. michiganensis 1616-3 using agar disc diffusion and microdilution assay. Efficacy of the extracts in the treatment of the tomato infected seeds by Clavibacter michiganensis in Petri plate assays. The chemical composition of MSEO revealed the presence of piperitenone oxide (39.11%), and germacrene D (13.6%) as a main compound. However, viridiflorol (28.82%) and Ɣ-gurjunene (14.61%) were the main compounds of CLEO. Among the extracts of Mentha suaveolens, the methanolic extract is the richest in phenol content (112.04 ± 2.98 mg EAG/g of extract), while the aqueous extract is richer on phenol content (76.98 ± 4.6 mg EAG/g of extract) for Cistus ladaniferus. The antibacterial activity showed important inhibition of plant pathogenic bacteria. The Petri plate assays indicated that 4×MIC of methanolic extract of C. ladaniferus showed the highest percentages of tomato germination seeds inoculated by C. michiganensis with rates of 77.77%. These findings suggest that methanolic extract of Cistus ladaniferus may be used as ecofriendly management of plant pathogens bacteria. However, further investigations concerning the identification of phenolic compounds of these plant extracts and the evaluation of antibacterial mechanism against plant pathogens bacteria are required.
Ethnopharmacological surveys conducted in Morocco allowed us to identify many plants species, the... more Ethnopharmacological surveys conducted in Morocco allowed us to identify many plants species, the most prescribed and used in traditional medicine to treat cancer. From these species, we chose to study Mesembryanthemum nodiflorum. We prepa-ed various extracts (alkaloid, cyclohexane, dichloromethane and methanolic) of the Mesembryanthemum nodiflorum aerial parts, the phytochemical studies revealed that the plant contains sterols, sapogenines, triterpenes, tannins and alkaloids. The chemical analysis of cyclohexane extract results in the identification of known con-stituents and the alkaloid extract has been studied by different chromatographic methods to isolate many fractions and to study their chemical composition. These investigations revealed the presence of hordenin. Cytotoxic activities were screened by an in vitro assay system of growth inhibition against two human cancer cell line, namely breast cancer cell line (MCF7) and cervix adenocarcinoma (HeLa), and the results demonst...
Objective: To investigate the cytotoxic, antioxidant and antibacterial activities of ethanolic an... more Objective: To investigate the cytotoxic, antioxidant and antibacterial activities of ethanolic and aqueous crude extracts of Nerium oleander (N. oleander) leaves. Methods: Cytotoxic activities were evaluated by WST-1 bioassay on two human cancer cell lines, namely human colon adenocarcinoma cell line HT-29 and human breast cancer cell line MDA-MB-231. The antioxidant property of N. oleander extracts was assessed by DPPH scavenging and β -carotene bleaching tests. The agar disc diffusion method was used for the determination of antimicrobial activity against different strains. Results: Using cell viability indices, the WST-1 test revealed that both extracts reduced cell viability in both cell lines. Our results also showed that aqueous extract was more active than ethanolic extract, with IC50 values of (1.67±0.22) μg/mL and (2.36±0.44) μg/mL on MDA-MB-231 cells, and (2.89±0.35) μg/mL and (5.09±0.52) μg/mL on HT29 cells, respectively. The study of the antioxidant activity showed that N. oleander extracts had a considerable scavenging capacity and exerted a significant preventive effect against the oxidation of β -carotene by the peroxide radicals. However, the antibacterial test showed that both ethanolic and aqueous extracts of N. oleander had a moderate antibacterial effect limited only to Gram-positive bacteria. Conclusions: Our results shows that N. oleander aqueous and ethanolic extracts have significant cytotoxic activities against tumor cell lines and possesses a strong antioxidant capacity, suggesting the presence of active compounds in N. oleander leaves that could be a potential source of phytochemicals with high medicinal value to be used in cancer treatment and prevention.
Using a cDNA probe, two genomic clones were obtained encoding the human tissue inhibitor of metal... more Using a cDNA probe, two genomic clones were obtained encoding the human tissue inhibitor of metalloproteinases-3 (TIMP-3). Analysis of these clones showed that they contained four distal exons and three introns of the gene. Although the intron-exon structure is similar to that of the timp1 gene, the first intron of the timp3 gene is much longer, being at least 17.5 kb in size.
Biocatalysis and agricultural biotechnology, Sep 1, 2020
Abstract This study aims to determine the phytochemical compounds of Cistus ladaniferus subsp. la... more Abstract This study aims to determine the phytochemical compounds of Cistus ladaniferus subsp. ladanifer and Mentha suaveolens and the investigations of their antibacterial effects against phytopathogenic bacteria. The chemical composition of Cistus ladaniferus subsp. ladanifer essential oils (CLEO) and Mentha suaveolens essential oils (MSEO) was determined using GC-MS analysis. The determination of phenolic content (TPC) of ethanolic, methanolic, and water plant extracts was realized by the Folin-Ciocalteu method. In vitro antibacterial activity was tested against Pseudomonas savastanoi pv. savastanoi 2636-40 and Clavibacter michiganensis subsp. michiganensis 1616-3 using agar disc diffusion and microdilution assay. Efficacy of the extracts in the treatment of the tomato infected seeds by Clavibacter michiganensis in Petri plate assays. The chemical composition of MSEO revealed the presence of piperitenone oxide (39.11%), and germacrene D (13.6%) as a main compound. However, viridiflorol (28.82%) and Ɣ-gurjunene (14.61%) were the main compounds of CLEO. Among the extracts of Mentha suaveolens, the methanolic extract is the richest in phenol content (112.04 ± 2.98 mg EAG/g of extract), while the aqueous extract is richer on phenol content (76.98 ± 4.6 mg EAG/g of extract) for Cistus ladaniferus. The antibacterial activity showed important inhibition of plant pathogenic bacteria. The Petri plate assays indicated that 4×MIC of methanolic extract of C. ladaniferus showed the highest percentages of tomato germination seeds inoculated by C. michiganensis with rates of 77.77%. These findings suggest that methanolic extract of Cistus ladaniferus may be used as ecofriendly management of plant pathogens bacteria. However, further investigations concerning the identification of phenolic compounds of these plant extracts and the evaluation of antibacterial mechanism against plant pathogens bacteria are required.
Ethnopharmacological surveys conducted in Morocco allowed us to identify many plants species, the... more Ethnopharmacological surveys conducted in Morocco allowed us to identify many plants species, the most prescribed and used in traditional medicine to treat cancer. From these species, we chose to study Mesembryanthemum nodiflorum. We prepa-ed various extracts (alkaloid, cyclohexane, dichloromethane and methanolic) of the Mesembryanthemum nodiflorum aerial parts, the phytochemical studies revealed that the plant contains sterols, sapogenines, triterpenes, tannins and alkaloids. The chemical analysis of cyclohexane extract results in the identification of known con-stituents and the alkaloid extract has been studied by different chromatographic methods to isolate many fractions and to study their chemical composition. These investigations revealed the presence of hordenin. Cytotoxic activities were screened by an in vitro assay system of growth inhibition against two human cancer cell line, namely breast cancer cell line (MCF7) and cervix adenocarcinoma (HeLa), and the results demonst...
Objective: To investigate the cytotoxic, antioxidant and antibacterial activities of ethanolic an... more Objective: To investigate the cytotoxic, antioxidant and antibacterial activities of ethanolic and aqueous crude extracts of Nerium oleander (N. oleander) leaves. Methods: Cytotoxic activities were evaluated by WST-1 bioassay on two human cancer cell lines, namely human colon adenocarcinoma cell line HT-29 and human breast cancer cell line MDA-MB-231. The antioxidant property of N. oleander extracts was assessed by DPPH scavenging and β -carotene bleaching tests. The agar disc diffusion method was used for the determination of antimicrobial activity against different strains. Results: Using cell viability indices, the WST-1 test revealed that both extracts reduced cell viability in both cell lines. Our results also showed that aqueous extract was more active than ethanolic extract, with IC50 values of (1.67±0.22) μg/mL and (2.36±0.44) μg/mL on MDA-MB-231 cells, and (2.89±0.35) μg/mL and (5.09±0.52) μg/mL on HT29 cells, respectively. The study of the antioxidant activity showed that N. oleander extracts had a considerable scavenging capacity and exerted a significant preventive effect against the oxidation of β -carotene by the peroxide radicals. However, the antibacterial test showed that both ethanolic and aqueous extracts of N. oleander had a moderate antibacterial effect limited only to Gram-positive bacteria. Conclusions: Our results shows that N. oleander aqueous and ethanolic extracts have significant cytotoxic activities against tumor cell lines and possesses a strong antioxidant capacity, suggesting the presence of active compounds in N. oleander leaves that could be a potential source of phytochemicals with high medicinal value to be used in cancer treatment and prevention.
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