A simple method for microorganism identification is proposed in this paper. In this method, no is... more A simple method for microorganism identification is proposed in this paper. In this method, no isolation or fractionation of microorganisms and no special search algorithm or database are needed. Experimentally, a sample of the unfractionated intact microorganism is subjected to brief on-slide proteolytic digestion and the digestion product ions detected in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) are selected for tandem mass spectrometry (MS/MS). Database searching using the MS/MS spectrum identifies the microorganism protein, and thereby, according to the source of the protein, the microorganism. The method was demonstrated to be feasible with enterobacteriophage MS2 as the model microorganism. The novel method is simple, rapid, and requires a small quantity of sample.
In this work ion mobility-mass spectrometry (IM-MS) coupled to an atmospheric solid analysis prob... more In this work ion mobility-mass spectrometry (IM-MS) coupled to an atmospheric solid analysis probe (ASAP) was used for the characterization of polymer blends involving biodegradable polymers (poly(lactic acid) (PLA), poly(butylene succinate) (PBS)) and poly(ethylene) (PE).
Tetraphenylporphinatoaluminum ethyl undergoes addition reaction toward vinyl ketone via a novel e... more Tetraphenylporphinatoaluminum ethyl undergoes addition reaction toward vinyl ketone via a novel enolate aluminum porphyrin as the intermediate. This reaction is remarkably accelerated upon irradiation with visible light retaining the reaction mode.
A simple method for microorganism identification is proposed in this paper. In this method, no is... more A simple method for microorganism identification is proposed in this paper. In this method, no isolation or fractionation of microorganisms and no special search algorithm or database are needed. Experimentally, a sample of the unfractionated intact microorganism is subjected to brief on-slide proteolytic digestion and the digestion product ions detected in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) are selected for tandem mass spectrometry (MS/MS). Database searching using the MS/MS spectrum identifies the microorganism protein, and thereby, according to the source of the protein, the microorganism. The method was demonstrated to be feasible with enterobacteriophage MS2 as the model microorganism. The novel method is simple, rapid, and requires a small quantity of sample.
In this work ion mobility-mass spectrometry (IM-MS) coupled to an atmospheric solid analysis prob... more In this work ion mobility-mass spectrometry (IM-MS) coupled to an atmospheric solid analysis probe (ASAP) was used for the characterization of polymer blends involving biodegradable polymers (poly(lactic acid) (PLA), poly(butylene succinate) (PBS)) and poly(ethylene) (PE).
Tetraphenylporphinatoaluminum ethyl undergoes addition reaction toward vinyl ketone via a novel e... more Tetraphenylporphinatoaluminum ethyl undergoes addition reaction toward vinyl ketone via a novel enolate aluminum porphyrin as the intermediate. This reaction is remarkably accelerated upon irradiation with visible light retaining the reaction mode.
Uploads
Papers by Carlos Afonso