Optimizing Soluble Cues for Salivary Gland Tissue Mimetics Using a Design of Experiments (DoE) Approach
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Department of Biomedical Engineering, University of Rochester, Rochester, NY 14627, USA
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Department of Biomedical Genetics, University of Rochester Medical Center, Rochester, NY 14642, USA
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Department of Environmental Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA
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Wilmot Cancer Institute, University of Rochester Medical Center, Rochester, NY 14642, USA
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Materials Science Program, University of Rochester, Rochester, NY 14627, USA
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Department of Chemical Engineering, University of Rochester, Rochester, NY 14627, USA
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Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY 14642, USA
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Department of Dermatology, University of Rochester Medical Center, Rochester, NY 14642, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Christine Delporte
Cells 2022, 11(12), 1962; https://doi.org/10.3390/cells11121962
Received: 18 May 2022
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Revised: 14 June 2022
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Accepted: 14 June 2022
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Published: 18 June 2022
The development of therapies to prevent or treat salivary gland dysfunction has been limited by a lack of functional in vitro models. Specifically, critical markers of salivary gland secretory phenotype downregulate rapidly ex vivo. Here, we utilize a salivary gland tissue chip model to conduct a design of experiments (DoE) approach to test combinations of seven soluble cues that were previously shown to maintain or improve salivary gland cell function. This approach uses statistical techniques to improve efficiency and accuracy of combinations of factors. The DoE-designed culture conditions improve markers of salivary gland function. Data show that the EGFR inhibitor, EKI-785, maintains relative mRNA expression of Mist1, a key acinar cell transcription factor, while FGF10 and neurturin promote mRNA expression of Aqp5 and Tmem16a, channel proteins involved in secretion. Mist1 mRNA expression correlates with increased secretory function, including calcium signaling and mucin (PAS-AB) staining. Overall, this study demonstrates that media conditions can be efficiently optimized to support secretory function in vitro using a DoE approach.
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Keywords:
salivary gland; design of experiments; Mist1; acinar cell; EGFR inhibitor
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MDPI and ACS Style
Piraino, L.R.; Benoit, D.S.W.; DeLouise, L.A. Optimizing Soluble Cues for Salivary Gland Tissue Mimetics Using a Design of Experiments (DoE) Approach. Cells 2022, 11, 1962. https://doi.org/10.3390/cells11121962
AMA Style
Piraino LR, Benoit DSW, DeLouise LA. Optimizing Soluble Cues for Salivary Gland Tissue Mimetics Using a Design of Experiments (DoE) Approach. Cells. 2022; 11(12):1962. https://doi.org/10.3390/cells11121962
Chicago/Turabian StylePiraino, Lindsay R., Danielle S.W. Benoit, and Lisa A. DeLouise. 2022. "Optimizing Soluble Cues for Salivary Gland Tissue Mimetics Using a Design of Experiments (DoE) Approach" Cells 11, no. 12: 1962. https://doi.org/10.3390/cells11121962
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