Supercritical Fluid Chromatography&mdash;Tandem Mass Spectrometry for Rapid Quantification of Pentacyclic Triterpenoids in Plant Extracts

Falev, Danil I.; Ovchinnikov, Denis V.; Voronov, Ilya S.; Faleva, Anna V.; Ul’yanovskii, Nikolay V.; Kosyakov, Dmitry S.

doi:10.3390/ph15050629

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Supercritical Fluid Chromatography—Tandem Mass Spectrometry for Rapid Quantification of Pentacyclic Triterpenoids in Plant Extracts

Nikolay V. Ul’yanovskii

and

Dmitry S. Kosyakov

Laboratory of Natural Compounds Chemistry and Bioanalytics, Core Facility Center “Arktika”, M.V. Lomonosov Northern (Arctic) Federal University, Northern Dvina Emb. 17, 163002 Arkhangelsk, Russia

Authors to whom correspondence should be addressed.

Academic Editors: Sabina Lachowicz-Wiśniewska and Jan Oszmianski

Pharmaceuticals 2022, 15(5), 629; https://doi.org/10.3390/ph15050629

Received: 19 April 2022 / Revised: 16 May 2022 / Accepted: 17 May 2022 / Published: 20 May 2022

(This article belongs to the Special Issue Liquid Chromatography in Analysis of Bioactive Compounds for Pharmaceuticals, Cosmetics, and Functional Food Interest)

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Abstract

Pentacyclic triterpenoids (PCTs) are a widely distributed class of plant secondary metabolites. These compounds have high bioactive properties, primarily antitumor and antioxidant activity. In this study, a method was developed for the quantitative analysis of pentacyclic triterpenoids in plants using supercritical fluid chromatography–tandem mass spectrometry (SFC-MS/MS). Separation of ten major PCTs (friedelin, lupeol, β-amyrin, α-amyrin, betulin, erythrodiol, uvaol, betulinic, oleanolic and ursolic acids) was studied on six silica-based reversed stationary phases. The best results (7 min analysis time in isocratic elution mode) were achieved on an HSS C18 SB stationary phase using carbon dioxide—isopropanol (8%) mobile phase providing decisive contribution of polar interactions to the retention of analytes. It was shown that the use of atmospheric pressure chemical ionization (APCI) is preferred over atmospheric pressure photoionization (APPI). The combination of SFC with APCI-MS/MS mass spectrometry made it possible to achieve the limits of quantification in plant extracts in the range of 2.3–20 μg·L⁻¹. The developed method was validated and tested in the analyses of birch outer layer (Betula pendula) bark, and licorice (Glycyrrhiza glabra) root, as well as lingonberry (Vaccinium vitis-idaea), cranberry (Vaccinium oxycoccos), apple (Malus domestica “Golden Delicious” and Malus domestica “Red Delicious”) peels. View Full-Text

Keywords: pentacyclic triterpenoids; plant feedstock; supercritical fluid chromatography; tandem mass spectrometry

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Falev, D.I.; Ovchinnikov, D.V.; Voronov, I.S.; Faleva, A.V.; Ul’yanovskii, N.V.; Kosyakov, D.S. Supercritical Fluid Chromatography—Tandem Mass Spectrometry for Rapid Quantification of Pentacyclic Triterpenoids in Plant Extracts. Pharmaceuticals 2022, 15, 629. https://doi.org/10.3390/ph15050629

AMA Style

Falev DI, Ovchinnikov DV, Voronov IS, Faleva AV, Ul’yanovskii NV, Kosyakov DS. Supercritical Fluid Chromatography—Tandem Mass Spectrometry for Rapid Quantification of Pentacyclic Triterpenoids in Plant Extracts. Pharmaceuticals. 2022; 15(5):629. https://doi.org/10.3390/ph15050629

Chicago/Turabian Style

Falev, Danil I., Denis V. Ovchinnikov, Ilya S. Voronov, Anna V. Faleva, Nikolay V. Ul’yanovskii, and Dmitry S. Kosyakov. 2022. "Supercritical Fluid Chromatography—Tandem Mass Spectrometry for Rapid Quantification of Pentacyclic Triterpenoids in Plant Extracts" Pharmaceuticals 15, no. 5: 629. https://doi.org/10.3390/ph15050629

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Supercritical Fluid Chromatography—Tandem Mass Spectrometry for Rapid Quantification of Pentacyclic Triterpenoids in Plant Extracts

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