Scandinavian journal of medicine & science in sports, Jan 31, 2015
We sought to identify and evaluate the tolerance to, and consequences of, short-term variations i... more We sought to identify and evaluate the tolerance to, and consequences of, short-term variations in training load in competitive weightlifters. Seven international-level lifters performed 1 week of initial training followed by 2 weeks of intensified (INT: +100%, 36.5 ± 11.3 × 10(3) kg/week) and 1 week of subsequently reduced (RED: -25%) training within their annual program. After INT, but not RED, 90 min of weightlifting increased mRNA levels of chemokine (C-C motif) ligand 4 (CCL4), chemokine (C-X-C motif) receptor 4 (CXCR4) and cellular stress-associated DNA-damage-inducible transcript 4 (DDIT4) in peripheral blood mononuclear cells by 40-240%. Resting- and weightlifting-induced changes in plasma protein carbonyls, indicative of oxidative stress, but not pro-inflammatory CCL4 concentrations differed between INT and RED. Symptoms of stress (Daily Analysis of Life Demands of Athletes questionnaire) were reported as worse than normal more frequently during INT and RED than initial tr...
The effects of 3 weeks of a 30% increase in training volume, followed by 1 week of reduced volume... more The effects of 3 weeks of a 30% increase in training volume, followed by 1 week of reduced volume (approximately 75%) training, on ergometer sprint performance and physiological recovery responses, were determined from an initial group of ten male and eight female elite rowers. No significant (p > 0.05) differences in mean (+/- SD) 500 m time trial performances were found when comparing 500 m times prior to, and after 3 weeks of overload training (89.4 +/- 7.3 s vs. 88.1 +/- 7.3 s), or from the end of the overload training to after the regeneration week (88.6 +/- 6.8s), or over the full 4-week overload-regeneration cycle. Peak and recovery heart rate responses to the test did not differ with training. However, recovery blood lactate concentrations increased, and blood ammonia decreased, after the third and fourth weeks of training. The results indicate that 3 weeks of overload training did not compromise ergometer sprint performance, but altered the metabolic responses during pas...
In stalk-eyed flies (Diopsidae) the eyes and antennae are laterally displaced at the ends of elon... more In stalk-eyed flies (Diopsidae) the eyes and antennae are laterally displaced at the ends of elongated eyestalks. Eyespan and the degree of sexual dimorphism in eyespan vary considerably between species and several sexually dimorphic species show sexual selection through female mate preference for males with exaggerated eyespan. The genes on which selection acts to regulate eyespan remain to be identified. This could be achieved by comparing gene expression during eyestalk development in males and females if the sex of pre-adult flies could be reliably assigned. Here we describe two techniques, one morphological and one microsatellite-based, that identify the sex of stalk-eyed fly larvae and pupae. We showed that genital discs of the stalk-eyed fly Teleopsis dalmanni have two highly distinct morphologies, compact ("C") and lobed ("L"). Segment composition (revealed by Engrailed expression) was consistent with C morphology being typical of males and L morphology o...
Philosophical Transactions of the Royal Society B: Biological Sciences, 2000
Salmonella, Shigella, Yersinia, Pseudomonas aeruginosa, enteropathogenic Escherichia coli and sev... more Salmonella, Shigella, Yersinia, Pseudomonas aeruginosa, enteropathogenic Escherichia coli and several plant-pathogenic Gram-negative bacteria use a new type of systems called 'type III secretion' to attack their host. These systems are activated by contact with a eukaryotic cell membrane and they allow bacteria to inject bacterial proteins across the two bacterial membranes and the eukaryotic cell membrane to reach a given compartment and destroy or subvert the target cell. These systems consist of a secretion apparatus made up of about 25 individual proteins and a set of proteins released by this apparatus. Some of these released proteins are 'effectors' that are delivered by extracellular bacteria into the cytosol of the target cell while the others are 'translocators' that help the 'effectors' to cross the membrane of the eukaryotic cell. Most of the 'effectors' act on the cytoskeleton or on intracellular signalling cascades. One of the proteins injected by the enteropathogenic E. coli serves as a membrane receptor for the docking of the bacterium itself at the surface of the cell.
In Drosophila, P-GAL4 enhancer trap lines can target expression of a cloned gene, under control o... more In Drosophila, P-GAL4 enhancer trap lines can target expression of a cloned gene, under control of a UASGAL element, to any cells of interest. However, additional expression of GAL4 in other cells can produce unwanted lethality or side-effects, particularly when it drives expression of a toxic gene product. To target the toxic gene product ricin A chain specifically to adult neurons, we have superimposed a second layer of regulation on the GAL4 control. We have constructed flies in which an effector gene is separated from UASGAL by a polyadenylation site flanked by two FRT sites in the same orientation. A recombination event between the two FRT sites, catalysed by yeast FLP recombinase, brings the effector gene under control of UASGAL. Consequently, expression of the effector gene is turned on in that cell and its descendants, if they also express GAL4. Recombinase is supplied by heat shock induction of a FLP transgene, allowing both timing and frequency of recombination events to be regulated. Using a lacZ effector (reporter) to test the system, we have generated labelled clones in the embryonic mesoderm and shown that most recombination events occur soon after FLP recombinase is supplied. By substituting the ricin A chain gene for lacZ, we have performed mosaic cell ablations in one GAL4 line that marks the adult giant descending neurons, and in a second which marks mushroom body neurons. In a number of cases we observed loss of one or both the adult giant descending neurons, or of subsets of mushroom body neurons. In association with the mushroom body ablations, we also observed misrouting of surviving axons.
In dipteran flies the adult abdominal epidermis is formed from small nests of diploid histoblast ... more In dipteran flies the adult abdominal epidermis is formed from small nests of diploid histoblast cells which spread out and replace the larval epidermis during metamorphosis. The pattern of nest outgrowth and fusion in Sarcophaga shows that the large dorsal hemitergite is normally formed by the two dorsal nests, the spiracle nest and part of the ventral nest (which also forms the hemisternite). By rotating the dorsal histoblast nests, we demonstrate that the adult segment border lies between the flexible 'intersegmental membrane' (ISM) and the naked anterior strip of tergite, the acrotergite. Deletion of histoblast nests often results in a corresponding deletion of adult structures, accompanied by enlargement of adjacent structures within the segment and in neighbouring segments. Pattern formation is not strictly coupled to cell division (as in imaginal discs), since the nests remaining after an ablation, in spreading to fill vacant areas, generate more cells and larger structures than normal. Nest deletions can also result in regeneration, with remaining nests forming additional structures in the dorsal-ventral or anterior-posterior axis of the segment. The deletion of strips of anterior and intersegmental larval epidermis without histoblasts results in the formation of double-posterior duplications of the adult hemitergite. Although these operations damage adjacent histoblast nests, several features of the results suggest that the duplications arise from the interaction (after healing) of histoblasts with larval cells which they would not normally encounter, leading to the intercalation of histoblast cells bearing intervening anterior-posterior positional values. A similar process of intercalation may occur in normal development, as the histoblasts spread from their local origins across the larval epidermal sheet, replacing the larval cells to form the entire epidermis of the adult segment.
Electronic literature is a term that encompasses artistic texts produced for printed media which ... more Electronic literature is a term that encompasses artistic texts produced for printed media which are consumed in electronic format, as well as text produced for electronic media that could not be printed without losing essential qualities. Some have argued that the essence of electronic literature is the use of multimedia, fragmentation, and/or non-linearity. Others focus on the role of computation and complex processing." Cybertext" does not sufficiently describe these systems. In this paper we propose that works of electronic ...
Journal of Neurology Neurosurgery and Psychiatry, 1998
OBJECTIVES(1) A biochemical investigation of the motor cortex in patients with incomplete spinal ... more OBJECTIVES(1) A biochemical investigation of the motor cortex in patients with incomplete spinal cord injury and normal control subjects using proton magnetic resonance spectroscopy (MRS). (2) To relate any altered biochemistry with the physiological changes in corticospinal function seen after spinal cord injury.METHODSa group of six patients with incomplete spinal cord injury who showed good recovery of motor function were
Page 1. Herpetologica, 42(2), 1986, 242-248 ) 1986 by The Herpetologists' League... more Page 1. Herpetologica, 42(2), 1986, 242-248 ) 1986 by The Herpetologists' League, Inc. CHRYSEMYS, PSEUDEMYS, TRACHEMYS (TESTUDINES: EMYDIDAE): DID AGASSIZ HAVE IT RIGHT? MICHAEL E. SEIDEL' AND HOBART M. SMITH2 IN. ...
Scandinavian journal of medicine & science in sports, Jan 31, 2015
We sought to identify and evaluate the tolerance to, and consequences of, short-term variations i... more We sought to identify and evaluate the tolerance to, and consequences of, short-term variations in training load in competitive weightlifters. Seven international-level lifters performed 1 week of initial training followed by 2 weeks of intensified (INT: +100%, 36.5 ± 11.3 × 10(3) kg/week) and 1 week of subsequently reduced (RED: -25%) training within their annual program. After INT, but not RED, 90 min of weightlifting increased mRNA levels of chemokine (C-C motif) ligand 4 (CCL4), chemokine (C-X-C motif) receptor 4 (CXCR4) and cellular stress-associated DNA-damage-inducible transcript 4 (DDIT4) in peripheral blood mononuclear cells by 40-240%. Resting- and weightlifting-induced changes in plasma protein carbonyls, indicative of oxidative stress, but not pro-inflammatory CCL4 concentrations differed between INT and RED. Symptoms of stress (Daily Analysis of Life Demands of Athletes questionnaire) were reported as worse than normal more frequently during INT and RED than initial tr...
The effects of 3 weeks of a 30% increase in training volume, followed by 1 week of reduced volume... more The effects of 3 weeks of a 30% increase in training volume, followed by 1 week of reduced volume (approximately 75%) training, on ergometer sprint performance and physiological recovery responses, were determined from an initial group of ten male and eight female elite rowers. No significant (p > 0.05) differences in mean (+/- SD) 500 m time trial performances were found when comparing 500 m times prior to, and after 3 weeks of overload training (89.4 +/- 7.3 s vs. 88.1 +/- 7.3 s), or from the end of the overload training to after the regeneration week (88.6 +/- 6.8s), or over the full 4-week overload-regeneration cycle. Peak and recovery heart rate responses to the test did not differ with training. However, recovery blood lactate concentrations increased, and blood ammonia decreased, after the third and fourth weeks of training. The results indicate that 3 weeks of overload training did not compromise ergometer sprint performance, but altered the metabolic responses during pas...
In stalk-eyed flies (Diopsidae) the eyes and antennae are laterally displaced at the ends of elon... more In stalk-eyed flies (Diopsidae) the eyes and antennae are laterally displaced at the ends of elongated eyestalks. Eyespan and the degree of sexual dimorphism in eyespan vary considerably between species and several sexually dimorphic species show sexual selection through female mate preference for males with exaggerated eyespan. The genes on which selection acts to regulate eyespan remain to be identified. This could be achieved by comparing gene expression during eyestalk development in males and females if the sex of pre-adult flies could be reliably assigned. Here we describe two techniques, one morphological and one microsatellite-based, that identify the sex of stalk-eyed fly larvae and pupae. We showed that genital discs of the stalk-eyed fly Teleopsis dalmanni have two highly distinct morphologies, compact ("C") and lobed ("L"). Segment composition (revealed by Engrailed expression) was consistent with C morphology being typical of males and L morphology o...
Philosophical Transactions of the Royal Society B: Biological Sciences, 2000
Salmonella, Shigella, Yersinia, Pseudomonas aeruginosa, enteropathogenic Escherichia coli and sev... more Salmonella, Shigella, Yersinia, Pseudomonas aeruginosa, enteropathogenic Escherichia coli and several plant-pathogenic Gram-negative bacteria use a new type of systems called 'type III secretion' to attack their host. These systems are activated by contact with a eukaryotic cell membrane and they allow bacteria to inject bacterial proteins across the two bacterial membranes and the eukaryotic cell membrane to reach a given compartment and destroy or subvert the target cell. These systems consist of a secretion apparatus made up of about 25 individual proteins and a set of proteins released by this apparatus. Some of these released proteins are 'effectors' that are delivered by extracellular bacteria into the cytosol of the target cell while the others are 'translocators' that help the 'effectors' to cross the membrane of the eukaryotic cell. Most of the 'effectors' act on the cytoskeleton or on intracellular signalling cascades. One of the proteins injected by the enteropathogenic E. coli serves as a membrane receptor for the docking of the bacterium itself at the surface of the cell.
In Drosophila, P-GAL4 enhancer trap lines can target expression of a cloned gene, under control o... more In Drosophila, P-GAL4 enhancer trap lines can target expression of a cloned gene, under control of a UASGAL element, to any cells of interest. However, additional expression of GAL4 in other cells can produce unwanted lethality or side-effects, particularly when it drives expression of a toxic gene product. To target the toxic gene product ricin A chain specifically to adult neurons, we have superimposed a second layer of regulation on the GAL4 control. We have constructed flies in which an effector gene is separated from UASGAL by a polyadenylation site flanked by two FRT sites in the same orientation. A recombination event between the two FRT sites, catalysed by yeast FLP recombinase, brings the effector gene under control of UASGAL. Consequently, expression of the effector gene is turned on in that cell and its descendants, if they also express GAL4. Recombinase is supplied by heat shock induction of a FLP transgene, allowing both timing and frequency of recombination events to be regulated. Using a lacZ effector (reporter) to test the system, we have generated labelled clones in the embryonic mesoderm and shown that most recombination events occur soon after FLP recombinase is supplied. By substituting the ricin A chain gene for lacZ, we have performed mosaic cell ablations in one GAL4 line that marks the adult giant descending neurons, and in a second which marks mushroom body neurons. In a number of cases we observed loss of one or both the adult giant descending neurons, or of subsets of mushroom body neurons. In association with the mushroom body ablations, we also observed misrouting of surviving axons.
In dipteran flies the adult abdominal epidermis is formed from small nests of diploid histoblast ... more In dipteran flies the adult abdominal epidermis is formed from small nests of diploid histoblast cells which spread out and replace the larval epidermis during metamorphosis. The pattern of nest outgrowth and fusion in Sarcophaga shows that the large dorsal hemitergite is normally formed by the two dorsal nests, the spiracle nest and part of the ventral nest (which also forms the hemisternite). By rotating the dorsal histoblast nests, we demonstrate that the adult segment border lies between the flexible 'intersegmental membrane' (ISM) and the naked anterior strip of tergite, the acrotergite. Deletion of histoblast nests often results in a corresponding deletion of adult structures, accompanied by enlargement of adjacent structures within the segment and in neighbouring segments. Pattern formation is not strictly coupled to cell division (as in imaginal discs), since the nests remaining after an ablation, in spreading to fill vacant areas, generate more cells and larger structures than normal. Nest deletions can also result in regeneration, with remaining nests forming additional structures in the dorsal-ventral or anterior-posterior axis of the segment. The deletion of strips of anterior and intersegmental larval epidermis without histoblasts results in the formation of double-posterior duplications of the adult hemitergite. Although these operations damage adjacent histoblast nests, several features of the results suggest that the duplications arise from the interaction (after healing) of histoblasts with larval cells which they would not normally encounter, leading to the intercalation of histoblast cells bearing intervening anterior-posterior positional values. A similar process of intercalation may occur in normal development, as the histoblasts spread from their local origins across the larval epidermal sheet, replacing the larval cells to form the entire epidermis of the adult segment.
Electronic literature is a term that encompasses artistic texts produced for printed media which ... more Electronic literature is a term that encompasses artistic texts produced for printed media which are consumed in electronic format, as well as text produced for electronic media that could not be printed without losing essential qualities. Some have argued that the essence of electronic literature is the use of multimedia, fragmentation, and/or non-linearity. Others focus on the role of computation and complex processing." Cybertext" does not sufficiently describe these systems. In this paper we propose that works of electronic ...
Journal of Neurology Neurosurgery and Psychiatry, 1998
OBJECTIVES(1) A biochemical investigation of the motor cortex in patients with incomplete spinal ... more OBJECTIVES(1) A biochemical investigation of the motor cortex in patients with incomplete spinal cord injury and normal control subjects using proton magnetic resonance spectroscopy (MRS). (2) To relate any altered biochemistry with the physiological changes in corticospinal function seen after spinal cord injury.METHODSa group of six patients with incomplete spinal cord injury who showed good recovery of motor function were
Page 1. Herpetologica, 42(2), 1986, 242-248 ) 1986 by The Herpetologists' League... more Page 1. Herpetologica, 42(2), 1986, 242-248 ) 1986 by The Herpetologists' League, Inc. CHRYSEMYS, PSEUDEMYS, TRACHEMYS (TESTUDINES: EMYDIDAE): DID AGASSIZ HAVE IT RIGHT? MICHAEL E. SEIDEL' AND HOBART M. SMITH2 IN. ...
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