Role of Xklp3, a Subunit of the Xenopus Kinesin II
Heterotrimeric Complex, in Membrane Transport between
the Endoplasmic Reticulum and the Golgi Apparatus
Nathalie Le Bot,* Claude Antony,‡ Jamie White,* Eric Karsenti,* and Isabelle Vernos*
*Cell Biology and Biophysics Program, European Molecular Biological Laboratory, D-69117 Heidelberg, Germany; and
‡
Institut Curie, 75248 Paris Cedex 05, France
modify proteins and lipids synthesized in the ER and
sort them to their final destination. The steady-state
size and function of the Golgi apparatus is maintained
through the recycling of some components back to the
ER. Several lines of evidence indicate that the spatial
segregation between the ER and the Golgi apparatus as
well as trafficking between these two compartments require both microtubules and motors. We have cloned
and characterized a new Xenopus kinesin like protein,
Xklp3, a subunit of the heterotrimeric Kinesin II. By
immunofluorescence it is found in the Golgi region. A
more detailed analysis by EM shows that it is associated
with a subset of membranes that contain the KDEL re-
I
n eukaryotic cells, membranous organelle components
and secretory proteins are synthesized and distributed
through a complex network of vesicles and tubules
that include the endoplasmic reticulum (ER) and the
Golgi apparatus. Proteins and lipids synthesized in the ER
are translocated in transport intermediates (tubular-vesicular structures) towards the Golgi apparatus, where they
are processed and sorted to specific subcellular destinations. The steady-state size and function of the Golgi apparatus depends on anterograde transport as well as on efficient recycling to the ER of some components that have
entered the secretory pathway. In higher eukaryotes, the
ER and Golgi apparatus are spatially segregated. The ER
extends from the nuclear envelope throughout the cytoplasm towards the cell periphery whereas the Golgi apparatus is concentrated at the cell center, around the centrosome. Several lines of evidence indicate that the spatial
segregation between the ER and the Golgi apparatus as
well as trafficking between them requires both microtuAddress correspondence to I. Vernos, Cell Biology and Biophysics Program, EMBL Heidelberg, Meyerhofstrasse 1, D-69117 Heidelberg, Germany. Tel.: (49) 6221-387-306. Fax: (49) 6221-387-306. E-mail: vernos@
embl-heidelberg.de
ceptor and are localized between the ER and Golgi apparatus. An association of Xklp3 with the recycling
compartment is further supported by a biochemical
analysis and the behavior of Xklp3 in BFA-treated
cells. The function of Xklp3 was analyzed by transfecting cells with a dominant-negative form lacking the motor domain. In these cells, the normal delivery of newly
synthesized proteins to the Golgi apparatus is blocked.
Taken together, these results indicate that Xklp3 is involved in the transport of tubular-vesicular elements
between the ER and the Golgi apparatus.
Key words:
Golgi • IC
Kinesin II • microtubules • ER •
bules and motors (for review see Lippincott-Schwartz,
1998).
It is now clear that a minus end–directed motor, dynein
or a dynein related protein, is required for positioning the
Golgi apparatus at microtubule minus ends (Theulaz et al.,
1992; Burkhardt et al., 1997; Corthesy-Harada et al., 1998).
A plus end–directed motor like conventional kinesin or a
kinesin-related protein, appears to be involved in the formation of tubules that emerge from the Golgi, and extend
towards the ER upon brefeldin (BFA)1 treatment. This
observation is compatible with the idea that plus end–
directed motors may drive the retrograde transport of vesicles from the Golgi to the ER (Lippincott-Schwartz et al.,
1995; Sciaky et al., 1997). Among the large number of
kinesin-like proteins found associated with membranes or
vesicles, two have been shown to be associated with the
Golgi apparatus. Rab–kinesin6 was identified as a Rab6
1. Abbreviations used in this paper: AMP-PNP, 59-adenylyllimido-diphosphate; BFA, brefeldin A; GFP, green fluorescent protein; GST, glutathione-S-transferase; HA, hemagglutinin; HP, Helix pomatia; IC, intermediate compartment; IFT, intraflagellar transport; KLP, kinesin-like
protein; MBP, maltose-binding protein; MT, microtubules; PDI, protein
disulfide isomerase; PNS, postnuclear supernatant.
The Rockefeller University Press, 0021-9525/98/12/1559/15 $2.00
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Abstract. The function of the Golgi apparatus is to
Materials and Methods
Isolation and Sequencing of Xklp3 cDNA
Partial Xklp3 cDNA clones were isolated from a Xenopus oocyte cDNA
library (Vernos et al., 1993). DNA was sequenced on both strands using
the T7, T3, and internal primers. The full-length cDNA for Xklp3 was reconstructed from three overlapping clones as follows: a Bluescript plasmid
(Stratagene, La Jolla, CA) carrying the 59 end sequence of Xklp3 cDNA
was cut at a BclI internal site and EcoRI and ligated with a 730-bp internal
fragment obtained by restriction digest of the second clone with BclIEcoRI and a 39 end fragment obtained from the third clone by digestion
with EcoR1. The final construct was sequenced. Sequence analysis and
comparisons were done with the DNASTAR program (London, UK) and
coiled-coil predictions according to Lupas et al. (1991).
Production of Xklp3 Fusion Proteins and Antibodies
Fragments of Xklp3 corresponding to either the motor domain (Xklp3-M,
nucleotides 217–1,248, amino acids 1–344) or part of the stalk and the full
tail (Xklp3-T, nucleotides 1,684–2,446, amino acids 489–744) were generated by PCR using Pwo polymerase (Boehringer Mannheim, Indianapolis,
IN) and inserted into the pGEX (Stratagene) and pMAL-c2 (New England Biolabs, Beverly, MA) vectors for expression in bacteria as recombinant proteins with the glutathione-S-transferase (GST) and the maltosebinding protein (MBP), respectively. The purified GST fusion proteins
(Smith and Jonshon, 1988) were used to immunize rabbits following standard procedures (Harlow and Lane, 1988). Antibodies were affinity purified against the corresponding MBP fusion protein (Boleti et al., 1996)
bound to CNBr-activated Sepharose (Pharmacia Biotech, Piscataway,
NJ). The MBP-tail fusion protein (amino acids 489–744) was used to immunize mice and a monoclonal antibody (mAb X3T-3A6) was prepared
following standard methods (Harlow and Lane, 1988).
(amino acid 345–744) was generated by PCR using the Pwo polymerase,
and cloned into the XhoI-BamH1 sites of pEGFP-C3 (Clontech, Palo
Alto, CA) to generate pEGFP-Xklp3-ST. The construct HA-Xklp3-ST
was generated by cutting out the green flourescent protein (GFP) epitope
from pEGFP-Xklp3-ST with Nhe1-Sca1 restriction enzymes and replacing
it with a 100-bp HA epitope generated by PCR from the vector pCMU-IV
(Nilsson et al., 1989). The full-length GFP-Xklp1 was cloned in pEGFPC3 and a fragment of the stalk and tail (nucleotides 2,370–3,740) was amplified by PCR and cloned in the vector pCDNA3.1 (Invitrogen, Carlsbad,
CA) to generate Xklp1-ST-myc. The stalk region of GalT, GalNacT2, or
Mannosidase II were fused to the NH2 terminus of GFP in the following
way. DNA fragments encoding the stalk regions were generated by PCR:
(a) a 361-bp fragment (amino acids 1–120) from human GalT cDNA
(GenBank/EMBL/DDBJ accession number X55415), (b) a 338-bp fragment (amino acids 1–114) from human GalNAcT2 cDNA (GenBank/
EMBL/DDBJ accession number X85019), and (c) a 348-bp fragment
(amino acids 1–116) from mouse a-mannosidase II cDNA (GenBank/
EMBL/DDBJ accession number X61172). The PCR fragments were digested and cloned in pEGFP–N1 (Clontech) to generate pGalT–GFP,
pGalNacT2–GFP, and pMannII–GFP. Vesicular stomatitus virus glycoprotein (VSV-G)–GFP was constructed by fusing GFP to the COOH terminus of the full-length VSV-G ts045 protein. A DNA fragment encoding
the VSV-G ts045 protein (GenBank/EMBL/DDBJ accession number
M11048) was generated by PCR and cloned in pEGFP-N1 to generate
pVSV-G–GFP. All constructs were sequenced and did not contain any
mutation altering the amino acid sequence.
Cell Lines and Transfection
Two frog cell lines were used, XL177 (Miller and Daniel, 1977), and A6
(Rafferty and Sherwin, 1969). Cells were grown at 238C in L-15 medium
with glutamine and 15% FCS. For transfection, A6 confluent cells were
split 1:5 onto coverslips placed in 24-well plates, at least 20 h before the
experiment. Cells were transfected with the Superfect reagent (QIAGEN,
Santa Clarita, CA) according to the manufacturer’s instructions. In short,
5 mg of plasmid DNA (Maxiprep; Qiagen) were used and the SuperfectDNA complex was left on the cells for 3 h before washing twice with 70%
PBS and then twice with complete medium. Cells were grown for an additional 12–20 h before observation.
Stable cell lines expressing Golgi markers were generated by transfecting A6 cells at 30% confluency on a 10-cm Petri dish with 10 mg of DNA.
Transfected cells were selected by adding 1 mg/ml of G418 (Geneticin;
GIBCO BRL Life Tech, Paisley, Scotland, UK) to the culture medium.
The GFP-positive cells were selected after 2 wk by flow cytometry on a
FACSort® (Becton Dickinson, Palo Alto, CA).
Antibodies and Reagents
Rhodamine- or FITC-conjugated lectin from Helix pomatia (Sigma
Chemical Co., St. Louis, MO) were resuspended in PBS and kept at 48C.
Nocodazole, cycloheximide, and BFA (Sigma Chemical Co.) were resuspended in DMSO, water, or methanol respectively, and kept at 2208C.
Nocodazole was used at a final concentration of 5 mM and BFA at 5 mg/
ml. The following antibodies were used: a mouse monoclonal anti-protein
disulfide isomerase (PDI) directed against the KDEL motif of the PDI
protein, from S. Fuller (European Molecular Biological Laboratory
[EMBL], Heidelberg, Germany); a sheep polyclonal anti–KDEL-receptor
from J. Füllekrug, (EMBL) and I. Majoul, (Max Planck Institute, Göttingen, Germany); a polyclonal anti-GFP from K. Sawin (Imperial Cancer
Research Fund, London, UK); a rabbit polyclonal anti-HA, from T. Nilsson (EMBL); a monoclonal anti-KRP85 and a polyclonal anti KAP-115
from J. Scholey, (University of California, Davis, CA); a monoclonal anti–
a-tubulin purchased from Sigma Chemical Co. FITC-, Texas red-, cy3-,
AMCA-conjugated secondary antibodies specific for rabbit or mouse
were purchased from Dianova (Hamburg, Germany). Gold-coupled protein A was purchased from J.W. Slot, (Utrecht University, Utrecht, The
Netherlands).
Immunofluorescence and Light Microscopy
A PCR fragment corresponding to the full stalk and tail domains of Xklp3
For immunofluorescence, fully confluent cells were split 1:2 onto coverslips and allowed to grow 24 h before fixation. Cells were washed in 70%
PBS, fixed either in methanol at 2208C for 10 min or with glutaraldehyde
as described in Boleti et al., (1997). The antibodies were diluted in PBS
with 2% bovine serum albumin (BSA) and 0.1% Triton X-100. Primary
antibodies were applied for 20 min at room temperature. The coverslips
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Expression Constructs for Eukaryotic Cells
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interacting protein but its role in the spatial organization
and dynamics of the Golgi has not been fully determined
yet (Echard et al., 1998). The mouse KIF1C, has recently
been described as a motor important for Golgi to ER vesicular transport induced by BFA treatment (Dorner et
al., 1998).
Here we report the characterization of Xklp3, a kinesinlike protein previously identified in Xenopus (Vernos et
al., 1993). Xklp3 sequence and biochemical features are
characteristic of the Kinesin II subfamily (for review see
Scholey, 1996). Kinesin II is a plus end–directed motor
formed by the association of three proteins: two closely
related kinesin-like proteins that interact through their
coiled-coil region and a third nonkinesin protein associated with their COOH terminus (Cole et al., 1993; Rashid
et al., 1995; Yamazaki et al., 1995, 1996; Wedaman et al.,
1996). Different members of this subfamily have been
shown to localize on vesicles and to be required for vesicular transport in the nervous system of the mouse (KIF3A/
KIF3B/KAP3) (Kondo et al., 1994), Drosophila (KLP64/
68) (Kondo et al., 1994; Pesavento et al., 1994) and Caenorhabditis elegans (OSM3) (Tabish et al., 1995), in ciliogenesis in sea urchin (KRP85/95/KAP115) (Morris and
Scholey, 1997), and flagella formation in Chlamydomonas
(FLA10) (Cole et al., 1998; Vashishtha et al., 1996). We
find that Xklp3 is associated with Golgi membranes, more
precisely with tubular-vesicular structures, where it colocalizes with recycling compartment components. Our data
indicate that its activity is required for normal trafficking
between the ER and the Golgi apparatus.
were washed three times for 5 min and secondary antibodies were then
applied for 15 min. The coverslips were washed three times for 5 min and
mounted in Mowiol (Hoechst, Frankfurt, Germany).
Confocal images were acquired using a confocal laser microscope Leica
TCS-NT (Deerfield, IL) equipped with an Ar/Kr laser triple line. Confocal series were collected and projections were made using the TCS-NT
software. For triple labeling experiments, we used a LSM-510 (Carl Zeiss
Inc., Thornwood, NY) confocal microscope equipped with a Ar/Kr and a
UV laser. In that case, single planes were collected. Image analysis was
performed using Adobe Photoshop 4.0 (Adobe Systems, Mountain
View, CA).
Electron Microscopy
Extracts and Immunoprecipitations
Interphase or cytosolic factor-arrested extracts from Xenopus eggs were
prepared as described by Murray (1991). Motor proteins were prepared
from egg extracts as described by Vernos et al. (1995). Whole cell extracts
were prepared according to Boleti et al. (1996). 10 mg of anti–Xklp3-tail
antibody coupled to 25 ml of Affiprep Protein A beads (Bio-Rad) were
used for immunoprecipitation from 100 ml of egg extract (Walczak et al.,
1996). Immunoprecipitations from transfected cells were performed in the
following way. One 10-cm Petri dish of cells transfected 20 h before the
experiment was incubated on ice with lysis buffer (25 mM Tris-Cl, pH 7.6,
200 mM NaCl, 1% Triton X-100, protease inhibitors). Cells were collected
by scraping and spun at 12,000 g for 10 min. The supernatant was incubated for 1 h at 48C with anti–GFP-coupled protein A–Sepharose (Pharmacia). The immunoprecipitate was washed three times (25 mM Tris-Cl
pH 7.6, 200 mM Nacl, 0.1% Triton X-100) and resuspended in sample
buffer before loading on SDS-PAGE and transfer on nitrocellulose for
Western blot analysis.
Results
Xklp3 cDNA and Protein Sequences
Using a PCR-based approach, four kinesin-like proteins
(Xklp1–Xklp4) were previously identified in Xenopus eggs
(Vernos et al., 1993). We screened a Xenopus oocyte cDNA
library with a probe corresponding to the motor domain of
Xklp3. We obtained a 3,762-bp sequence from three overlapping clones containing an initiation codon at position
215, a stop codon at position 2,447, and a polyadenylation
signal at position 3,716. The 2232-bp open reading frame
encoded a protein of 744 residues with a calculated Mr of
85 kD.
The deduced protein sequence showed that Xklp3 had a
conventional kinesin-like protein (KLP) organization with
the NH2-terminal motor domain, an a-helical region predicted to be involved in coiled-coil interactions and a globular COOH-terminal tail domain (Fig. 1 A). Alignment of
the Xklp3 motor domain sequence with other KLP sequences had previously shown that Xklp3 was closely related to Drosophila KLP4 and KLP5 (renamed KLP64D
and KLP68D), both members of the KIF3/KRP85/95 or
Kinesin II subfamily (Vernos et al., 1993; Pesavento et al.,
1994; Scholey, 1996). Xklp3 full-length sequence showed
that it also shared a high degree of similarity to these
KLPs outside the motor domain. The highest scores were
obtained with KIF3B (Yamazaki et al., 1995), with 90%
amino acid similarity in the motor domain, 85% in the
stalk, and 74% in the tail (Fig. 1 B). This high level of conservation suggests that Xklp3 is probably the Xenopus
counterpart of mouse KIF3B.
Xklp3 Is Part of a Trimeric Complex
Biochemical fractionations were done in the following way. A 10,000 g in-
To characterize Xklp3 we raised two polyclonal antibodies: one against the motor domain (anti–Xklp3-M) and
another against the COOH-terminal tail domain (anti–
Xklp3-tail). Both affinity-purified antibodies, as well as a
monoclonal antibody, mAb X3T-3A6, recognized a single
band with an apparent Mr of 95 kD on immunoblots of extracts from Xenopus eggs and from two different Xenopus
cell lines, XL177 and A6 (Fig. 2 A). We then looked at
Xklp3 ATP-dependent microtubule-binding activity. In
Le Bot et al. Role of Xklp3 in Trafficking between the ER and the Golgi
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Biochemical Fractionation
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Cells were grown on a 10-cm Petri dish and fixed by adding to the medium
an equivalent volume of 4% paraformaldehyde in 0.2 M sodium phosphate buffer, pH 7.4, for 1 h. The medium was then replaced with a fresh
solution of 2% paraformaldehyde in 0.1 M sodium phosphate buffer and
incubated for one more hour. Cells were collected by careful scraping and
processed for cryosectioning according to Slot et al., (1991). The cryosections were retrieved with a 1:1 solution of 2.3 M sucrose and 2% methylcellulose according to Liou et al. (1996). Double immunogold labelings
were performed as described previously (Slot et al., 1991). The monoclonal antibody X3T-3A6 was used in combination with a rabbit anti–
mouse linker antibody, and then 10 nm of protein A–gold. The polyclonal
anti–Xklp3-tail antibody was combined with 10 nm of protein A–gold.
The polyclonal anti–KDEL-receptor or the polyclonal anti-GFP were
combined with 5 nm of protein A–gold. Sections were stained, embedded
in 2% methylcellulose/ 0.3% uranyl acetate, and then viewed in a Phillips
CM120 electron microscope (Eindhoven, The Netherlands) at 80 KV.
The labeling densities of Xklp3 over the KDEL-receptor structures, the
Golgi stacks (GalT GFP-positive), and the ER (PDI-positive) and the cytoplasm, were determined by the point-hit method (Weibel, 1979). 20 images were analyzed per marker. The KDEL-receptor compartment was
defined as the tubular-vesicular structures areas marked by the erd-2 antibody, the Golgi stacks as membrane structures containing three or more
cisternae and positive for the GalT–GFP, and the ER structures as the
membranes marked by the anti-PDI antibody. The cross-section surface
area for each compartment was measured using a square-lattice grid with
a spacing d 5 12.5 mm to count the points P corresponding to the grid line
intersections comprised within the boundaries of the respective compartment. The surface area is then given by SPxd2/mag2 (in mm2). The labeling
density of Xklp3 in each compartment was calculated by dividing the
number of gold particles that fall into the boundaries of each type of structure by the respective surface area. To evaluate Xklp3 density in the cytoplasm, we positioned a box corresponding to 10 points (d 5 12.5 mm) on
randomly selected areas of cytoplasm deprived of any membrane structures. We are conscious that this method might overestimate Xklp3 density in the cytoplasm, and for this reason, we counted a greater number of
micrographs (n 5 30). Background labeling was calculated by counting
the number of gold particles detected on the mitochondria. It was usually
z1 gold particle per mm2 both for the polyclonal anti–Xklp3-tail and the
mab-X3-T-3A6.
terphase egg extract was diluted three times with acetate buffer (Reinsch
and Karsenti, 1997) and spun at 100 Kg for 30 min to obtain a membrane
and a cytosol fraction. The membranes were resuspended in 2 M of sucrose in acetate buffer and loaded at the bottom of a sucrose step gradient
(1.3/0.86/0.5/0.25 M sucrose in acetate buffer). The gradient was centrifuged overnight in a SW60 rotor (Beckman Instrs., Palo Alto, CA) at 300
Kg. The four layers were collected, centrifuged through a sucrose cushion,
and then the pellets resuspended in one-tenth of the initial volume.
Cytosol fractionation from cells was performed in the following way.
Cells grown on six 500 cm2 plates were washed, scraped in 70% PBS, and
then incubated in cytochalasin D. After centrifugation they were resuspended in KHEM (35 mM KCl, 50 mM Hepes, 5 mM EGTA, 1 mM
MgCl2, 3 mg/ml cycloheximide and 1 mM DTT, and protease inhibitors).
Cells were broken by 10–15 passages through a ball-bearing homogenizer
(8.0004-mm clearance) and centrifuged at 2,000 g to remove nuclei, heavy
mitochondria, and unbroken cells. The postnuclear supernatant (PNS)
was loaded at the top of a sucrose step gradient (0.25/0.5/0.86/1.3 M in
KHEM) in SW40 tubes, and then centrifuged for 4 h at 200 Kg. 1-ml fractions were collected. The proteins contained in 200 ml of each fraction
were precipitated with methanol/chloroform and loaded on SDS-PAGE
followed by immunoblotting as described previously (Vernos et al., 1996).
The Journal of Cell Biology, Volume 143, 1998
1562
Figure 2. Xklp3 is a subunit
of the Kinesin II heterotrimeric complex and is associated to Golgi membranes.
(A) Characterization of antibodies against Xklp3. Antibodies raised against the
NH2-terminal (a-Motor) and
the COOH-terminal (a-Tail)
region of Xklp3 recognize a
single band of z95 kD on
Western blots of Xenopus
low-speed
egg
extract,
XL177, and A6 cell extracts.
The monoclonal anti-tail,
mAb X3T-3A6, recognizes a
single band at 95 kD in lowspeed egg extract. (B) Xklp3
cosediment with microtubules in the presence of
AMP-PNP, and is released
from the MT by addition of
ATP. Xenopus egg extract
(High Speed Extract) was
used to prepare a fraction enriched in motor proteins
(Materials and Methods).
The high speed extract, the
MT pellet in presence of
AMP-PNP, the motor proteins released from MT by
ATP, and the MT pellet after
ATP elution were analyzed
by Western blot and probed
with the anti–Xklp3-tail antibody. Most of Xklp3 is released from the microtubules
by 10 mM ATP. (C and D)
Xklp3 is a subunit of a het-
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Figure 1. Primary sequence, structural features of Xklp3 and sequence similarity to Kinesin II subfamily members. (A) Xklp3 protein sequence. Italics and
boldface, conserved regions corresponding to the sequence used for the design
of the degenerated oligos used in the original screen for Xenopus KLPs; double
underline, ATP-binding site residues; underline, kinesin motor conserved MTbinding site; dotted underline, region rich in charged amino acids that is present
in other members of the family. This region has been proposed to favor heterodimerization over homodimerization. These sequence
data are available from GenBank/EMBL/DDBJ under accession number AJ009839. (B) Output of the pepcoil program showing the
probability of formation of coiled-coil interactions. Below a schematic representation of Xklp3 is shown, with regions predicted to be
coiled coil (white). Asterisk, region rich in charged amino acids that interrupts the coiled-coil predicted domain and is present in other
members of the family. The ATP (dark line) and MT binding sites (white rectangle) in the motor region are indicated. (C) Similarity between protein sequences of Xklp3 and the other Kinesin II family members. Schematic representation of the Kinesin II heterotrimeric
complex, composed of two closely related kinesins (Klp1 and Klp2) and a third nonmotor subunit (KAP). Each kinesin-like protein has
three subdomains: motor (red), stalk (green), and tail (blue). The third nonmotor subunit (i.e., KAP115 in sea urchin, KAP3 in mouse) is
represented as a gray oval interacting with the tails. Xklp3 sequence is taken as a reference and the numbers correspond to the percentage of similarities found in the different domains between Xklp3 and the corresponding KLP. The first group comprises KLPs more
closely related to Xklp3, the second group comprises the second KLP subunit from the complex. In the third subgroup, Xklp3 sequence
is compared with the mouse KIF3C, a motor able to heterodimerize with KIF3A but not with KIF3B. We place other members from
this subfamily in the fourth group.
Xklp3 Is Associated with Membranes from the
Golgi Network
We first determined the expression pattern of Xklp3 by
Western blotting of different Xenopus tissues. We found
Xklp3 in oocytes, eggs, testis, brain, XL177, and A6 cells.
It was also present, although in lower amounts, in kidney
and stomach and at very low levels in heart, lung, and
muscle (data not shown). These data are consistent with
the ubiquitous tissue distribution found for Kinesin II in
mouse (Yamazaki et al., 1995).
We then examined the subcellular localization of Xklp3
in XL177 and A6 Xenopus cells. Immunofluorescence
analysis of interphase XL177 cells with the anti–Xklp3-tail
antibody revealed a bright staining concentrated in a perinuclear or juxtanuclear position (Fig. 2 E). The structure
stained by the antibody appeared as a network of interconnected tubules reminiscent of the Golgi apparatus. In
addition to this bright network, we could detect in some
cells a light labeling of vesicle-like structures scattered
throughout the cytoplasm. In mitotic cells, the labeled network disappeared and was replaced by a punctuate staining in the cytoplasm enriched around the spindle poles
(data not shown). This staining pattern was similar to that
observed in Xenopus dividing embryos (data not shown).
We did not see any enrichment of labeled structures at the
spindle equator in anaphase embryonic cells, as it has been
reported for sea urchin Kinesin II (Henson et al., 1995).
To determine if the interphase network stained by the
anti–Xklp3-tail antibody corresponded to the Golgi apparatus, we performed double immunofluorescence labeling on
XL177 cells with the anti–Xklp3-tail antibody and different
markers of Golgi membranes. Xklp3 staining colocalized
extensively with the Helix pomatia (HP) lectin that binds
N-acetyl-galactosamine residues generated at the first step
of O-linked glycosylation (Pavelka and Ellinger, 1985;
Roth, 1984), in the Golgi apparatus (Fig. 3 A). It colocalized
perfectly with the KDEL-receptor (revealed by an anti–
KDEL-receptor antibody, Fig. 3 A), an itinerant Golgi
protein which recycles KDEL-containing ligands from the
Golgi back to the ER (Lewis and Pelham, 1992). The
KDEL-receptor is usually localized in the cis-Golgi and
the intermediate compartment (Tang et al., 1993). We also
observed a perfect colocalization of Xklp3 with b-COP a
component of the COP I complex implicated in transport
steps between the ER and the Golgi (data not shown).
To further define the localization of Xklp3 and because
of the lack of cross-reacting antibodies, we prepared three
stable lines of Xenopus A6 cells expressing GFP-tagged
versions of Golgi resident enzymes: mannosidase II–GFP
(MannII–GFP), galactosyl-N-acetyl transferase (GalNacT2–
GFP), and galactosyltransferase (GalT–GFP). In mammalian cells it has been shown that addition of the GFP tag
does not interfere with the targeting of these proteins to
the Golgi apparatus (for review see Lippincott-Schwartz
et al., 1998). We checked by immunofluorescence with
FITC conjugated HP and the anti–KDEL-receptor antibody that all the GFP-tagged proteins localized to the
Golgi network in Xenopus cells as well (data not shown).
In addition, in vivo time-lapse confocal microscopy (data
not shown) and EM (see below) also confirmed that the
behavior of these proteins in Xenopus cells was similar to
what has been described for mammalian cells (Sciaky et
al., 1997; Shima et al., 1997). By immunofluorescence,
Xklp3 colocalized with all GFP-tagged markers in the juxtanuclear network (GalNacT2–GFP, GalT–GFP [Fig. 3 B]
and MannII–GFP [data not shown]); in addition, Xklp3
was present on dimly labeled vesicular structures throughout the cytoplasm.
To determine whether Xklp3 was also present in the
ER, we stained cells with the anti–Xklp3-tail antibody and
an anti-PDI, generated against the KDEL motif of lumenal ER resident proteins (Tooze et al., 1989). Anti–Xklp3tail and anti-PDI antibodies did not stain the same network of membranes (Fig. 4). To examine whether Xklp3
was present on ER-to-Golgi or TGN to plasma membrane
erotrimeric complex, homologous to the Kinesin II complex. (C) Coomassie-stained gel of an immunoprecipitation from low-speed Xenopus egg extract using the anti–Xklp3-tail antibody. Two proteins (80 and 110 kD, arrows) coimmunoprecipitate with Xklp3 (asterisk).
A control was done using protein A beads alone (Mock lane), similar results were obtained using nonspecific rabbit IgG. Arrowhead,
lower bands that represent the antibodies’ heavy chains. (D) Western Blot of the immunoprecipitated fraction. The 95-kD band is recognized by the anti–Xklp3-tail antibody (a-Xklp3). The 85-kD band is recognized by an antibody against SpKRP85, the second KLP
subunit of Kinesin II (a-KRP85), and the 110-kD band by an antibody against SpKAP115, the third nonmotor subunit (a-KAP115). (E)
Double immunofluorescence on Xenopus XL177 cells with the anti–Xklp3-tail (left panel, green) and an anti-tubulin antibody (middle
panel, red). Xklp3 localizes to an extended juxtanuclear network. The nuclear pattern observed here is not representative of Xklp3
staining (see other figures where it is not present). Bar, 10 mm.
Le Bot et al. Role of Xklp3 in Trafficking between the ER and the Golgi
1563
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egg extracts, Xklp3 copelleted with endogenous taxol-stabilized microtubules in the presence of 59-adenylyllimidodiphosphate (AMP-PNP) and was released from the microtubule pellet by 10 mM ATP (Fig. 2 B), similar to the
behavior of kinesin and other KLPs (Bloom and Endow,
1994).
Most members of the Kinesin II subfamily form heterotrimeric complexes (Scholey, 1996). To determine whether
this was also the case for Xklp3, we used the anti–Xklp3tail antibody to immunoprecipitate the complex from Xenopus egg extracts. Three proteins of 95, 80, and 100 kD
coimmunoprecipitated in equimolar amounts as estimated
by SDS-PAGE gel (Fig. 2 C, arrows). Immunoblots of the
immunoprecipitated material showed that the 95-kD band
was Xklp3. The 80-kD band was recognized by an antibody against sea urchin KRP85 (the second motor subunit,
Cole et al. [1993]) and the 100-kD band by an antibody
against kinesin associated protein KAP115 (the third nonmotor subunit, Wedaman et al. [1996]). We conclude that
Xklp3 is part of an heterotrimeric complex in Xenopus
that is homologous to sea urchin Kinesin II and mouse
KIF3A/KIF3B/KAP3 complexes (Fig. 1 B). Since in sea
urchin and mouse, Kinesin II (Cole et al., 1993; Yamazaki
et al., 1995) is a plus end–directed motor, it is likely that
this is also the case for Xenopus Kinesin II.
Figure 4. Xklp3 does not colocalize with
ER the PDI or with post-Golgi structures
en route to the plasma membrane. (Top)
XL177 cells stained with the anti–Xklp3tail antibody and an anti-KDEL signal antibody (anti-PDI). (Bottom) A6 cells transiently transfected with VSV-G–GFP and
stained with the anti–Xklp3-tail antibody.
Xklp3 colocalizes with VSV-G–GFP in
the Golgi apparatus but not in structures
at the vicinity of the plasma membrane,
thus indicating that Xklp3 is not associated with post-Golgi membranes. Red,
Xklp3; green, corresponding marker. Bar,
10 mm.
The Journal of Cell Biology, Volume 143, 1998
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Figure 3. Xklp3 colocalization with different Golgi markers. (A) Double immunofluorescence on XL177 cells with the anti–
Xklp3-tail antibody and antibodies against
different markers for the Golgi apparatus,
as indicated. Xklp3 colocalizes partially
with the lectin HP and completely with the
KDEL-receptor. (B) A6 cells stably expressing GalT–GFP or GalNacT2–GFP
were fixed and processed for immunofluorescence with the anti–Xklp3-tail antibody. Xklp3 colocalizes with both markers. Red, Xklp3; green, corresponding
marker. Bars, 10 mm.
vesicles, we transiently transfected A6 cells with a VSVG–GFP. VSV-G–GFP is exported from the ER to the
Golgi apparatus where it is processed before being included in post-TGN membrane-bound structures and transported towards the plasma membrane (Arnheiter et al.,
1984; Kreis and Lodish, 1986). Xklp3 and VSV-G–GFP
colocalized extensively in the Golgi region, but no colocalization was seen in the peripheral (i.e., ER or post-TGN)
vesicle-like structures or at plasma membrane extensions.
Altogether these results indicated that in A6 and XL177
Xenopus cell lines, Xklp3 was associated with Golgi mem-
branes, but did not reveal to which specific Golgi subcompartment.
Upon BFA Treatment of the Cells, Xklp3 Does Not
Behave As a Golgi Stack Protein
In a first attempt to determine whether Xklp3 was associated with Golgi stacks, we examined its relocalization after
BFA treatment. BFA is used to discriminate secretory
pathway subcompartments. This drug prevents the binding
of peripheral coatomer proteins type 1 (COPI) proteins to
Le Bot et al. Role of Xklp3 in Trafficking between the ER and the Golgi
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Figure 5. Xklp3 and the KDEL
receptor have a similar behavior upon BFA treatment.
(A) A6 cells expressing Gal
NacT2–GFP
constitutively
were incubated with 5 ag/ml
of BFA for 5 min or 1 h and
then stained with the anti–
Xklp3-tail antibodies. After
5 min of BFA treatment,
GalNacT2–GFP starts to be
redistributed into the ER often appearing on tubular
structures. The anti–Xklp3tail antibody gives a punctuate staining dispersed in the
cytoplasm, often associated
to but not overlapping with
the GalNacT2–GFP staining
(enlarged region). After 15
min, the structures stained by
GalNacT2–GFP and the vesicular structures stained with
the anti–Xklp3-tail antibody
are completely distinct (1
hour, enlarged region). (B)
XL177 cells were incubated
with 5 mg/ml of BFA for 5
min or 1 h and double stained
with the anti–Xklp3-tail and
the anti–KDEL-receptor antibodies. The KDEL-receptor and Xklp3 colocalize on
vesicular structures at early
time points (enlarged region,
overlay, 5 min). After 1 h of
treatment, vesicular structures positive for both Xklp3
and the KDEL-receptor are
still seen, but part of this
colocalization is lost (enlarged
region, overlay, 1 hour). Red,
Xklp3; green, corresponding
marker. Bar, 10 mm.
Golgi membranes leading to membrane tubulation and redistribution of Golgi resident proteins into the ER (Doms
et al., 1989; Scheel et al., 1997). However, some recycling
proteins such as the KDEL-receptor, do not redistribute
into the ER but are dispersed into vesicular structures
throughout the cytoplasm (Tang et al., 1995; Fullekrüg et
al., 1997). We verified that BFA treatment of Xenopus A6
and XL177 cells did result in a cytoplasmic redistribution
of b-COP, a component of the COPI complex (data not
shown). After 5 min of BFA treatment, Xklp3 was still
present in some perinuclear structures and appeared in
punctuate structures dispersed throughout the cytoplasm
whereas the GalNacT2–GFP was associated with long tubular processes (Fig. 5 A). After 1 h of treatment, Xklp3
was associated with dispersed peripheral vesicular structures while GalNacT2–GFP had completely redistributed
into the ER (Fig. 5 A). The lack of redistribution to the
ER at later time points suggested that Xklp3 was not truly
associated with the Golgi stacks. We then compared Xklp3
distribution to that of the KDEL receptor in BFA-treated
cells (Fig. 5 B). During the first 30 min of BFA treatment,
Xklp3 and the KDEL receptor colocalized on some vesicular structures (Fig. 5 B, 5 min, enlarged region). After 1 h
of treatment, some of the vesicles stained by the antiXklp3 antibody were still positive for the KDEL receptor
(Fig. 5 B, 1 hour, enlarged region) although a fraction of
them were not. These results suggested that Xklp3 was associated with membrane structures distinct from the Golgi
stacks and somehow related to the recycling compartment.
Xklp3 Is Localized on Tubular-vesicular Elements
between the ER and the Golgi
The Journal of Cell Biology, Volume 143, 1998
1566
To identify the nature of the Golgi elements to which
Xklp3 was associated, we examined thin frozen sections of
cells using immunogold EM. Previous EM studies have
shown that the Golgi apparatus is composed of stacks of
flattened cisternae enriched in glycosylating enzymes, and
tubulovesicular structures associated with the rims of the
stacks probably involved in specific transport steps (Rambourg and Clermont, 1996). We first looked at the relative
localization of Xklp3 and the GalT–GFP chimera using
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Figure 6. Xklp3 localizes to
tubular-vesicular structures
between the ER and the
Golgi. (A) Double immunogold labeling of Xklp3
(mAb X3T-3A6, 10-nm gold)
and the KDEL-receptor (polyclonal anti-erd2, 5-nm gold)
on cryosections from A6GalT–GFP cells. Xklp3 is
present on tubular-vesicular
structures (arrows and arrowheads) around the Golgi-cisternae (G) where it colocalizes with the KDEL-receptor
(arrows). Similar results are
obtained with the polyclonal
anti-tail (see Table I for
quantification). (B) Double
immunogold labeling of Xklp3
(monoclonal anti–Xklp3-tail,
mAb X3T-3A6, 10-nm gold)
and the GalT–GFP (polyclonal anti-GFP, 5-nm gold).
GalT–GFP is localized exclusively to the Golgi cisternae
and there is no clear colocalization with Xklp3 (arrowheads) When Xklp3 is found
on the Golgi stacks, it is always at its periphery, on budding-like structures (A–C).
Similar results are obtained
with the polyclonal anti-tail
(see Table I for quantification). (C) Double immunogold labeling of Xklp3
(polyclonal anti–Xklp3-tail,
10-nm gold, arrows and arrowheads) and PDI (monoclonal anti-PDI, 5-nm gold). The PDI is found in cisternae throughout the cytoplasm. Xklp3 is often seen on one side of these ER membrane structures (arrows) (see Table I for quantification). Bar, 100 nm.
These data represent the average of 20 micrographs for each structure (n 5 30 for the
cytoplasm). Numbers in parentheses give the standard error of the mean.
tion). The rest of Xklp3 was localized partly in the cytoplasm or associated with unlabeled membranous elements
some of which were morphologically similar to the ER. A
double-labeling experiment with an anti-PDI antibody
showed Xklp3 was localized as well on structures bearing
PDI (Fig. 6 C, arrows) (Table I, quantification). EM studies have shown that the KDEL-receptor is distributed
among the cis-Golgi, tubular-vesicular elements of the intermediate compartment and ER membranes (Griffiths et
al., 1994). Thus the distribution of Xklp3 is similar to that
of an intermediate compartment protein.
Le Bot et al. Role of Xklp3 in Trafficking between the ER and the Golgi
1567
Table I. Quantification of Xklp3 Labeling on Different
Membrane Structures
Compartment
KDEL-receptor structures
Golgi stacks (GalT–GTP)
ER structures (PDI)
Cytoplasm
Polyclonal anti–Xklp3tail gold/mm2 membrane
Monoclonal X3T-3A6
gold/mm2 membrane
11.6 (6 1.8)
2.9 (6 0.6)
6 (6 1)
11.6 (6 1.6)
2.8 (6 0.6)
—
3.7 (6 0.8)
3.5 (6 0.7)
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the monoclonal anti–Xklp3-tail (mAb X3T-3A6) and a
polyclonal anti-GFP antibody followed by detection with
protein A coupled to 10-nm (Xklp3) and 5-nm (GFP)
gold, respectively. GalT–GFP was only found in the
stacked cisternae of the Golgi complex as expected (Fig. 6
B, 5-nm gold), whereas Xklp3 was associated with tubularvesicular structures sometimes in the proximity of the stacks
(Fig. 6, A and B, arrows and arrowheads). A similar experiment using a polyclonal anti–KDEL-receptor and the
same anti Xklp3 antibodies revealed that Xklp3 (10-nm
gold) was found on tubulovesicular structures positive for
the KDEL-receptor (Fig 6 A, arrows) (Table I, quantifica-
Figure 7. Xklp3 is associated to heavy membranes. (A) Xklp3 is
found in a membrane fraction prepared from egg extracts. Western blot of a high-speed cytosol and the corresponding membrane fraction with the anti–Xklp3-tail antibody. Equivalent volumes from each fraction were loaded on each lane. (B) The
membrane fraction shown in A was resuspended in 2 M sucrose,
loaded at the bottom of a sucrose step gradient, and then centrifuged to equilibrium. Equivalent volumes of the different fractions were loaded on PAGE and transferred to nitrocellulose.
The Western blot was probed with the anti–Xklp3-tail antibody.
(1) 2 M/1.3 M sucrose (Bottom); (2) 1.3 M/0.86 M (heavy membranes, zER); (3) 0.86 M/0.5 M (membranes zheavy Golgi); (4)
0.5 M/0.25 M (light membranes). (C) A fraction of Xklp3 is associated to heavy membranes from A6 cells extract. Cytosol (PNS)
was prepared from A6 cells expressing GFP–mannosidase II constitutively as described in Materials and Methods. The PNS was
then loaded on a sucrose step gradient (sucrose concentrations
are written above fraction numbers; arrows, interface between
two sucrose concentrations). Fractions were collected and run on
PAGE. The Western blot was probed sequentially with the following antibodies: anti–Xklp3-tail, anti-PDI (as an ER marker),
anti-GFP (to detect the Golgi marker mannosidase II–GFP), and
anti-tubulin (as a nonmembrane-associated molecule). Xklp3 is
present in soluble fractions 1–4. In addition, there are two peaks
of Xklp3 in fractions where heavy membranes sediment: one
around fraction 7 corresponding to membranes enriched in mannosidase II–GFP, and another around fraction 9–10 corresponding to membranes enriched in PDI.
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Figure 8. Effect of the expression of Xklp3–ST on Golgi staining by HP lectin. (A) The Xklp3–ST construct is able to bind to the endogenous Xklp3– KLP partner. A6 cells were transiently transfected with GFP–Xklp3-ST. The cytosol was used for immunoprecipitation with an anti-GFP antibody (lanes GFP–Xklp3-ST). The immunoprecipitate was probed with the anti-GFP and the anti-KRP85 antibodies. As a control we repeated this experiment with nontransfected cells (lanes None). Lane E is the crude extract fraction and lane
IP the immunoprecipitate (loaded five times more than the extract). In transfected cells the anti-GFP antibody immunoprecipitates
The Journal of Cell Biology, Volume 143, 1998
1568
Xklp3 Is Associated with Heavy Membrane Fractions
Xklp3 Is Required for Normal Trafficking between
the ER and the Golgi
The previous results pointed to a role for Xklp3 in the
movement of tubular-vesicular elements between the ER
and Golgi apparatus. To investigate the function of Xklp3,
we transfected Xenopus cells with a plasmid expressing
a truncated form of Xklp3 with either GFP (pEGFP–
Xklp3-ST) or a hemagglutinin (HA) tag (pHA–Xklp3-ST)
replacing the whole motor domain. Immunoprecipitation
experiments with an anti-GFP antibody showed that the
mutant protein could inhibit Kinesin II function by competing with the endogenous Xklp3 for the binding to the
85-kD subunit of the Kinesin II heterotrimeric complex
GFP–Xklp3-ST and a protein recognized by the anti-KRP85 antibody, probably the KLP partner for Xklp3. (B) Expression of Xklp3–ST
has no effect on microtubules. A6 cells transiently transfected with GFP–Xklp3-ST were fixed and processed for immunofluorescence
with an anti-tubulin antibody. Cells expressing Xklp3-ST have a normal microtubule network focused at the MTOC. (C) Suppression of
Golgi staining by the lectin from Helix pomatia in cells expressing Xklp3–ST. A6 cells were transiently transfected with GFP–Xklp3-ST
(asterisks), fixed, and then stained with rhodamine-labeled HP lectin. A projection of six sections taken with a LSCM is shown. In cells
transfected with Xklp3–ST, the lectin no longer recognizes any identifiable Golgi structure. In contrast, the Golgi apparatus of non
transfected cells is strongly stained. As a control we transfected A6 cells with GFP–Xklp1 (a Xenopus mitotic motor, asterisk). In these
cells, the Golgi apparatus was normally stained by the HP lectin (bottom). (D) Quantification of the defective Golgi staining by the HP
lectin. A6 cells were transiently transfected by GFP–Xklp3-ST, HA–Xklp3-ST, or GFP–Xklp1. 50–100 transfected cells were counted
for each experiment. Bars correspond to the percentage of cells in each category. The standard deviations are shown (n 5 4 for GFP–
Xklp3-ST, HA–Xklp3-ST and n 5 1 for GFP–Xklp1). White bars, normal Golgi HP lectin staining; black bars, abnormal or lack of staining. Note that 60–70% of Xklp3-ST–transfected cells have an abnormal Golgi staining, whereas this happens only in 10% of GFPXklp1–transfected cells. (E) The lack of Golgi staining by the HP lectin in cells expressing Xklp3–ST may reflect a defective transport
from the ER to the Golgi. A6 cells expressing GalNacT2–GFP constitutively were transiently transfected with HA–Xklp3-ST (asterisks). Cells were fixed and double stained with a polyclonal anti-HA antibody, then visualized by an AMCA conjugated anti-rabbit antibody, and with rhodamine-labeled HP lectin. Left, red, HA–Xklp3-ST; green, GalNacT2–GFP. Right, HP lectin staining. The Golgi apparatus appears normal but is not stained by the rhodamine-labeled HP lectin. (F) In A6 cells, the lectin from HP binds to proteins en
route along the secretory pathway. A6 cells expressing GalNacT2–GFP constitutively were treated for 3 h with cycloheximide, fixed,
and then stained with rhodamine-labeled HP lectin. As above, the fluorescent lectin does not stain the Golgi apparatus. Bars, 10 mm.
Le Bot et al. Role of Xklp3 in Trafficking between the ER and the Golgi
1569
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Membranes from the Golgi stacks, the ER, and recycling
compartments behave differently upon sedimentation or
floatation on sucrose gradients. We first separated the
membranes from cytosolic proteins in egg extracts using a
one step centrifugation, and further fractionated them by
floatation. Approximately 20–25% of Xklp3 cosedimented
with the membrane fraction (Fig. 7 A). We found Xklp3
associated with membranes that floated at the 1.3 M–0.86 M
sucrose interface (like ER membranes) and at the 0.86
M–0.5 M sucrose interface (like Golgi membranes, Fig. 7
B). To further characterize the membrane fractions with
which Xklp3 cosedimented, we prepared PNS from A6
cells stably transfected with MannII–GFP. The homogenates were then loaded on a sucrose step gradient (Fig. 7
C). Xklp3 was found in large amounts at the 0.25 M–0.5 M
sucrose interface, probably corresponding to the soluble
form of the trimeric complex. In addition, Xklp3 was
found in two heavier fractions, at the 0.5 M–0.86 M sucrose interface which corresponded to a peak of Golgi
membranes as revealed by the presence of MannII–GFP
(Fig. 7 C, fraction 7, Xklp3, MannII–GFP) and at the 0.86
M–1.3 M sucrose interface which corresponded to a peak
of ER/intermediate compartment as revealed by the presence of PDI. These results indicated that part of Xklp3
was associated with heavy membranes from both Golgi
and ER/intermediate compartment.
(Fig. 1 B and Fig. 8 A). By immunofluorescence, both fusion proteins were mostly found distributed throughout
the cytoplasm and not associated with the Golgi apparatus
(Fig. 8, B and C). This could be explained in two ways: either the mutant protein could not be targeted to the vesicles to which the wild-type protein binds normally or the
mutant protein prevented the localization of the Kinesin II
complex to its target vesicles. In either case, this mutant
clearly acted as a poison for the endogenous Kinesin II
complex. Indeed, a similar construct has been used successfully to study Kinesin II dependent movement of the
melanosomes in Xenopus melanophores (Tuma et al.,
1998).
We first tried to detect an effect on one of the Golgi
functions, glycosylation. The lectin from HP binds specifically to N-acetyl-galactosamine residues added at the beginning of the O-glycosylation. In A6 cells, it labels the
Golgi apparatus, thus indicating that this step is occurring
in the Golgi or in its direct vicinity. We stained the
transfected cells with fluorescently labeled HP lectin and
quantified the effect by scoring cells exhibiting a normal
staining of the Golgi versus cells with no Golgi structure
stained. Strikingly, the HP lectin no longer labeled the
Golgi apparatus in 60–70% of Xklp3-ST–transfected cells
(Fig. 8 C, asterisks; Fig. 8 D, quantification). This effect
was similar in cells transfected with either tagged version
of the mutant (Fig. 8 C). Transfected cells had a normal
distribution of microtubules (MT) (Fig. 8 B), ER, mitochondria, and g-tubulin (data not shown). As a control, we
transfected cells with a plasmid expressing GFP-tagged
Xklp1, a nuclear Xenopus kinesin-like protein involved in
mitosis. Only 12% of the transfected cells had an abnormal HP lectin staining (Fig. 8 C, arrow; Fig. 8 D, quantification).
The effect of the Xklp3 mutant on lectin staining pattern
suggested a defect in Golgi glycosylation function. The
Xklp3 mutant could either affect directly the general
structure of the Golgi or more specifically, the localization
of the O-glycosylating enzymes. Alternatively, the mutant
protein could affect the global transport of proteins from
the ER to the Golgi.
To examine the morphology of the Golgi apparatus in
the absence of Kinesin II function, we transfected cells
stably expressing Golgi–GFP markers with the Xklp3
mutant. In these cells, the Golgi apparatus remained intact
although the GFP staining was often weaker than in nontransfected cells (Fig. 8 E, asterisks). In the transfected
cells, the HP lectin did not recognize any structure (Fig. 8
E, top) or very weakly the Golgi apparatus (Fig. 8 E,
bottom). This suggested that the normal trafficking of
proteins through the Golgi complex was altered. To test
The Journal of Cell Biology, Volume 143, 1998
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Figure 9. Newly synthesized GalNacT2–GFP and GalT–GFP proteins do not reach the Golgi apparatus in cells expressing Xklp3–ST.
(A) A6 cells were transiently cotransfected with HA–Xklp3-ST and GalNacT2–GFP. Transfected cells (asterisks) were detected by immunofluorescence and confocal series were taken. A projection of six sections is presented. In cotransfected cells, GalNacT2–GFP is
seen in aggregates in the cytoplasm and in the ER. In control cells, cotransfected with Xklp1-ST–myc and GalNacT2–GFP, the GFP
marker localized to the Golgi apparatus. Bar, 10 mm. (B) Quantification of the defect of Golgi–GFP markers localization. A6 cells were
transiently transfected with the different combinations indicated. Cells were scored as either having the GFP Golgi marker localized
normally to the Golgi, to the ER and Golgi, or to the ER and aggregates. Bars show the percentage of cells in each category and the error bars show the standard deviation (n 5 3). (C) Cells transiently cotransfected with HA–Xklp3-ST and GalNacT2–GFP exhibit an abnormal lectin staining. GalNacT2-GFP–expressing cells are detected by the GFP tag fluorescence. Cells were double labeled with a rabbit anti-HA antibody (then visualized by an AMCA conjugated anti-rabbit antibody) and the fluorescent lectin HP. Single confocal
planes are presented. Left, note that two cells transfected only with GalNacT2–GFP (asterisks) show normal labeling of the Golgi by the
HP lectin. Cotransfected cells show GalNacT2–GFP either in ER and Golgi (left panel, arrow 1) or in ER and aggregates (left panel, arrow 2; right panel). ∫Bar, 10 mm.
Discussion
cles that disperse throughout the cytoplasm in a way similar to some IC markers (Tang et al., 1993; Griffith et al.,
1994). Second, biochemical fractionation of the cytosol
shows that Xklp3-associated membranes are found in fractions containing Golgi and ER markers. This behavior has
been reported for several intermediate compartment proteins (Tang et al., 1993; Griffith et al., 1994). Third, by
electron microscopy, Xklp3 is present mainly on tubularvesicular structures that are sometimes closely associated
with the Golgi stacks and are often stained by an anti–
KDEL-receptor antibody. From all these data we conclude that, in Xenopus fibroblasts, Kinesin II is associated
with a specific subset of membranes localized between the
ER and the Golgi.
Xklp3 and Golgi Function
Using a mutant form of Xklp3 that functions as a dominant negative, we show that the Xenopus Kinesin II is involved in an essential aspect of Golgi function. When the
mutant is overexpressed, the HP lectin no longer detects
N-acetyl-galactosaminyl residues on proteins in the Golgi
apparatus. This indicates that Xklp3 is required for the
normal localization of the O-glycosylating enzymes and/or
for the normal delivery of their substrates. In fact, Xklp3 is
required for the localization of newly synthesized proteins
to the Golgi apparatus. When the Xklp3 mutant is coexpressed with GFP–Golgi markers, these are mislocalized
to the ER or to aggregates in the cytoplasm and do not
reach the Golgi apparatus. Strikingly, in this case the staining of glycosilated residues by the HP lectin is also aberrant. This indicates that there is a correlation between the
delivery of newly synthesized proteins to the Golgi and the
defect in lectin staining. This interpretation is further supported by the finding that the inhibition of protein synthesis results in the absence of Golgi staining by the lectin.
These results lead us to propose that Xklp3 function is
required for the transport of newly synthesized proteins
from the ER to the Golgi apparatus.
Mode of Action for Xklp3 between the ER
and the Golgi
By immunofluorescence, we show that Xklp3 is associated
with the Golgi apparatus. We see a extensive colocalization with GFP chimeras targeted to the stack or to the
trans-face of the Golgi, as well as with the KDEL-receptor, a recycling molecule enriched on the cis-face of the
Golgi. On the other hand, we find that Xklp3 does not
colocalize with markers for the ER nor with VSV-G–labeled
peripheral structures en route along the secretory pathway. Several experiments suggest that Xklp3 is actually
present on membranes between the ER and the Golgi, on
the so-called intermediate compartment (IC). First, upon
treatment with BFA, Xklp3 remains associated with vesi-
Given the association of Xklp3 with membranes localized
between the ER and the Golgi apparatus, the effect of the
mutant on the transport of proteins from the ER to the
Golgi apparatus and the directionality of movement of
Xklp3 along microtubules (plus end–directed), we propose
two mechanisms of action for Xklp3 along the secretory
pathway. We know that traffic between the ER and the
Golgi apparatus involves an anterograde and a retrograde
transport of vesicles which requires dynein and probably
plus end–directed motors like kinesin. Dynein carries
transport intermediates from the ER exit sites towards the
MTOC where they integrate into the Golgi apparatus
(Presley et al., 1997). Although this has not been demonstrated, it is possible that a plus end–directed motor counteracts the activity of dynein during anterograde transport
contributing to the steady-state organization of the Golgi
network. Indeed, using an antisense approach, Feiguin et
al. (1994) have demonstrated that conventional kinesin
may be needed for organizing the Golgi network in astrocytes. Because expressing the Xklp3 mutant interferes
Le Bot et al. Role of Xklp3 in Trafficking between the ER and the Golgi
1571
In this study, we report the cloning and characterization of
a subunit of the Xenopus heterotrimeric Kinesin II. Localization and functional data indicate that Xenopus Kinesin
II plays an important role between the ER and the Golgi.
Evidence for an Association of Xklp3 with the
Intermediate Compartment
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the hypothesis that the HP lectin detected N-acetylgalactosamine residues of cargo proteins en route along the
secretory pathway during their transit through the Golgi
apparatus, we blocked protein synthesis in cells expressing
constitutively GalNacT2–GFP with cycloheximide to prevent the delivery of newly synthesized proteins to the
Golgi apparatus. Under these conditions, the HP lectin did
not label the Golgi apparatus although the Golgi was
structurally intact as indicated by the GalNacT2–GFP fluorescence (Fig. 8 F). This result was very similar to the one
we obtained in cells transfected with the mutant Xklp3
and suggested that the mutant form of Xklp3 was interfering with the delivery of newly synthesized proteins to the
Golgi apparatus.
To examine whether the mutant form of Xklp3 blocked
transport between the ER and the Golgi, we cotransfected
cells with HA–Xklp3-ST and GalNacT2–GFP or GalT–
GFP. The GFP markers localized exclusively to the Golgi
in more than 80% of control cells. Cells expressing both
HA–Xklp3-ST and one GFP Golgi marker showed a
strong defect in the localization of the GFP marker to the
Golgi (Fig. 9, A–C). Interestingly, the mislocalization of
the Golgi GFP marker following co-transfection with the
Xklp3-ST was associated with an aberrant HP lectin staining (Fig. 9 C). The phenotype observed could not be due
to a general effect on the MTs because the cells still had
a normal MT network focused at the MTOC (data not
shown). As a control, we repeated the co-transfection experiment using a plasmid expressing a fragment of Xklp1
lacking the motor domain (Xklp1-ST–myc) similar to
HA–Xklp3-ST. The Xklp1-ST–myc lacks also the NLS,
and as a consequence remains in the cytoplasm, as HA–
Xklp3-ST does. In cells expressing both Xklp1-ST–myc
and GalNacT2–GFP, the GFP marker localized properly
to the Golgi apparatus (Fig. 9 A).
Therefore, the results obtained with the dominant-negative form of Xklp3 indicate that Kinesin II is required for
the proper transport and localization of newly synthesized
proteins, to the Golgi apparatus.
Kinesin II Functions
In various organisms, Kinesin II family members have
been reported to associate with different types of membrane structures. In mouse neuronal cells, KIF3A/KIF3B/
KAP3 is found on vesicles, distinct from synaptic vesicles,
in the cell body and along the axon (Yamazaki et al.,
1995). It has been recently reported that mouse KIF3C
(able to heterodimerize with KIF3A but not with KIF3B
[the Xklp3 mouse homologue]) colocalizes with Giantin, a
Golgi membrane protein, in spinal cord neuronal cells
(Yang and Goldstein, 1998). In sea urchin embryos, Kinesin II is associated with vesicles enriched at the poles, and
at the spindle interzone (Henson et al., 1995). Antibody
microinjection experiments have shown that, in sea urchin,
KRP95/KRP85/KAP115 is necessary during embryogenesis for correct delivery of ciliary components (Morris and
Scholey, 1997). In Chlamydomonas, the fla10 gene product, the KRP85 homologue is associated to protein complexes beneath the flagellar membrane and mutant phenotypes suggest that FLA10 is involved in the intraflagellar
transport (IFT) of protein complexes required for flagellar
formation (Cole et al., 1998; Kozminski et al., 1995). In
Xenopus, Kinesin II is present as well on purified melano-
The Journal of Cell Biology, Volume 143, 1998
somes prepared from skin melanophores and a Xklp3 mutant protein equivalent to the Xklp3-ST used in this study
inhibits the outward movement of melanosomes (Rogers
et al., 1997; Tuma et al., 1998).
All these results indicate that Kinesin II associates with
different organelles depending on the cell type and may
play a role in a wide variety of processes. It is possible that
this functional diversity is related to the heterotrimeric nature of the molecule and to the existence of isoforms or to
the association of a core molecule with various proteins.
Future work attempting to understand how it is targeted to
a specific organelle and function should be of great interest.
It is worth noting that, in many cases, Kinesin II seems
to antagonize the activity of dynein. In Chlamydomonas,
cytoplasmic dynein is required to counteract FLA10
driven force and move the IFT particles back to the basal
body of the flagella (Pazour et al., 1998). Xenopus melanosomes carry both the Kinesin II complex and cytoplasmic
dynein. In this report we present evidence that Xenopus
Kinesin II is involved in some step of the secretory pathway which also requires the activity of dynein.
This report is one of the first indications, together with
the recent finding about the Rab6 interacting kinesin, and
the mouse KIF1C, of the targeting of a KLP to a specific
subcompartment of the secretory pathway and of a function for this KLP in this subcompartment. It is possible
that different types of motors will be found targeted to
specific domains of the secretory pathway, participating to
the sorting process. The identification of such molecules
may help to understand better the complexity of membrane traffic between the ER and the Golgi apparatus.
The fact that the rabkinesin is targeted to the Golgi apparatus through a small G protein (Rab 6) raises the possibility that there is a whole pattern of localization of motors to
specific membranes by adaptors like the G proteins. In this
sense it is interesting to note that the human homologue of
KAP3/KAP115, the third non motor subunit of Kinesin II,
was cloned looking for proteins interacting with a small
GTP-binding protein dissociation factor (Smg-GDS) (Shimizu et al., 1996). Smg GDS itself regulates the interaction
of various small G proteins with membranes and might
link the heterotrimeric kinesin to specific membrane cargoes via G protein interactions. Therefore, it will be of interest to examine whether different KLPs are involved in
the transport of specific membrane compartments in the
secretory pathway.
We would like to thank J. Füllekrug, S. Fuller, I. Majoul, T. Nilsson, K.
Sawin, and J.M. Scholey, for the gift of antibodies. At EMBL, we would
like to thank F. Senger and H. Wilhelm for their invaluable help in the
production of antibodies, A. Atzberger for help with the cell sorter, and S.
Röttger for help with the EM quantification. We also thank S. Reinsch, J.
Mata, T. Nilsson, and T. Wittmann for discussions and advice, and J.
Mata, T. Wittmann, and M. Zerial for critical reading of the manuscript.
Received for publication 14 August 1998 and in revised form 5 November
1998.
References
Arnheiter, H., M. Dubois-Dalcq, and R.A. Lazzarini. 1984. Direct visualization
of protein transport and processing in the living cell by microinjection of specific antibodies. Cell. 39:99–109.
Bloom, G.S., and S.A. Endow. 1994. Kinesins. In Protein Profile. Vol. 1. E.
Shaterline, editor. Academic Press, London. 1089–1105.
1572
Downloaded from http://rupress.org/jcb/article-pdf/143/6/1559/1851342/9808066.pdf by guest on 31 July 2023
with the normal delivery of newly synthesized proteins to
the Golgi, it is possible that Xklp3 participates in such a
process.
On the other hand, two observations suggest that kinesin and/or KLP(s) are involved in the retrograde transport
of vesicles from the Golgi apparatus to the ER. The inhibition of kinesin blocks the formation of tubular-vesicular
structures between the Golgi and ER in the presence of
BFA (Lippincott-Schwartz et al., 1995) and live studies
have shown that the KDEL-receptor (important for the
recycling of ER resident proteins) is associated with tubular-vesicular membrane structures forming at a velocity
compatible with the involvement of a kinesin related protein (Sciaky et al., 1997). Therefore the steady-state size,
location, and function of the Golgi apparatus may be
strongly dependent on the relative rates of anterograde
and retrograde transport. As a consequence, we can imagine that blocking a retrograde transport motor, here the
Kinesin II, could also affect the anterograde pathway. An
apparently surprising result is that even though inactivation of Xklp3 leads to a strong defect in ER to Golgi transport, it does not affect dramatically overall Golgi morphology, at least during the time course of our experiments.
There are several possible explanations to this result.
However, we favor the idea that blocking the retrograde
transport results in freezing the flux of components
throughout the secretory pathway, temporarily maintaining an apparently intact Golgi structure. It is also possible
that by inhibiting Kinesin II, we block one specific part of
the transport and that other motors keep other aspects of
the secretory pathway active.
At this point, we emphasize that these two possibilities
are only possible interpretations of our results, based both
on our localization and functional data. More work will be
required to establish exactly how the Xenopus Kinesin II
is involved in the transport of components between the
ER and the Golgi apparatus.
(IFT). J. Cell Biol. 141:979–992.
Pesavento, P.A., R.J. Stewart, and L.S. Goldstein. 1994. Characterization of the
KLP68D kinesin-like protein in Drosophila: possible roles in axonal transport. J. Cell Biol. 127:1041–1048.
Presley, J.F., N.B. Cole, T.A. Schroer, K. Hirschberg, K.J. Zaal, and J. Lippincott-Schwartz. 1997. ER-to-Golgi transport visualized in living cells. Nature.
389:81–85.
Rafferty, K.A., Jr., and R.W. Sherwin. 1969. The length of secondary chromosomal constrictions in normal individuals and in a nucleolar mutant of Xenopus laevis. Cytogenetics. 8:427–438.
Rambourg, A., and Y. Clermont. 1996. Three-dimensional strucure of the Golgi
apparatus in mammalian cells. In The Golgi Apparatus. E.G. Berger and J.
Roth, editors. Birkhauser, Basel, Switzerland. 37–61.
Rashid, D.J., K.P. Wedaman, and J.M. Scholey. 1995. Heterodimerization of
the two motor subunits of the heterotrimeric kinesin, KRP85/95. J. Mol.
Biol. 252:157–162.
Reinsch, S., and E. Karsenti. 1997. Movement of nuclei along microtubules in
Xenopus egg extracts. Curr. Biol. 7:211–214.
Rogers, S.L., I.S. Tint, P.C. Fanapour, and V.I. Gelfand. 1997. Regulated bidirectional motility of melanophore pigment granules along microtubules in
vitro. Proc. Natl. Acad. Sci. USA. 94:3720–3725.
Roth, J. 1984. Cytochemical localization of terminal N-acetyl-d-galactosamine
residues in cellular compartments of intestinal goblet cells: implications for
the topology of O-glycosylation. J. Cell Biol. 98:399–406.
Scheel, J., R. Pepperkok, M. Lowe, G. Griffiths, and T.E. Kreis. 1997. Dissociation of coatomer from membranes is required for brefeldin A-induced transfer of Golgi enzymes to the endoplasmic reticulum. J. Cell Biol. 137:319–333.
Scholey, J.M. 1996. Kinesin II, a membrane traffic motor in axons, axonemes,
and spindles. J. Cell Biol. 133:1–4.
Sciaky, N., J. Presley, C. Smith, K.J. Zaal, N. Cole, J.E. Moreira, M. Terasaki,
E. Siggia, and J. Lippincott-Schwartz. 1997. Golgi tubule traffic and the effects of brefeldin A visualized in living cells. J. Cell Biol. 139:1137–1155.
Shima, D.T., K. Haldar, R. Pepperkok, R. Watson, and G. Warren. 1997. Partitioning of the Golgi apparatus during mitosis in living HeLa cells. J.Cell Biol.
137:1211–1228.
Shimizu, K., H. Kawabe, S. Minami, T. Honda, K. Takaishi, H. Shirataki, and
Y. Takai. 1996. SMAP, a Smg GDS-associating protein having Arm repeats
and phosphorylated by Src tyrosine kinase. J. Biol. Chem. 271:27013–27017.
Slot, J.W., H.J. Geuze, S. Gigengack, G.E. Lienhard, and D.E. James. 1991. Immunolocalization of the insulin regulatable glucose transporter in brown adipose tissue of the rat. J. Cell Biol. 113:123–135.
Smith, D.B., and K.S. Johnson. 1988. Single step purification of polypeptides
expressed in Escherichia coli as fusions with glutathione S-transferase. Gene.
67:31–40.
Tabish, M., Z.K. Siddiqui, K. Nishikawa, and S.S. Siddiqui. 1995. Exclusive expression of C. elegans osm-3 kinesin gene in chemosensory neurons open to
the external environment. J. Mol. Biol. 247:377–389.
Tang, B.L., S.H. Low, and W. Hong. 1995. Differential response of resident
proteins and cycling proteins of the Golgi to brefeldin A. Eur. J. Cell Biol.
68:199–205.
Tang, B.L., S.H. Wong, X.L. Qi, S.H. Low, and W. Hong. 1993. Molecular cloning, characterization, subcellular localization and dynamics of p23, the mammalian KDEL receptor. J. Cell Biol. 120:325–328.
Tooze, J., H.F. Kern, S.D. Fuller, and K.E. Howell. 1989. Condensation-sorting
events in the rough endoplasmic reticulum of exocrine pancreatic cells. J.
Cell Biol. 109:35–50.
Tuma, M.C., A. Zill, N. Le Bot, I. Vernos, and V. Gelfand. Kinesin II is the microtubule motor protein responsible for pigment dispersion in Xenopus melanophores. J. Cell Biol. 143:1547–1558.
Vashishtha, M., Z. Walther, and J.L. Hall. 1996. The kinesin-homologous protein encoded by the Chlamydomonas FLA10 gene is associated with basal
bodies and centrioles. J. Cell Sci. 109:541–549.
Vernos, I., J. Heasman, and C. Wylie. 1993. Multiple kinesin-like transcripts in
Xenopus oocytes. Dev. Biol. 157:232–239.
Vernos, I., J. Raats, T. Hirano, J. Heasman, E. Karsenti, and C. Wylie. 1995.
Xklp1, a chromosomal Xenopus kinesin-like protein essential for spindle organization and chromosome positioning. Cell. 81:117–127.
Walczak, C., T.J. Mitchison, and A.B. Desai. 1996. XKCM1: a Xenopus kinesinrelated protein that regulates microtubule dynamics during mitotic spindle
assembly. Cell. 84:37–47.
Wedaman, K.P., D.W. Meyer, D.J. Rashid, D.G. Cole, and J.M. Scholey. 1996.
Sequence and submolecular localization of the 115-kD accessory subunit of
the heterotrimeric kinesin-II (KRP85/95) complex. J. Cell Biol. 132:371–380.
Weibel, E.R. 1979. Stereologial Methods. I. Practical Methods for Morphometry. Academic Press, London, UK.
Yamazaki, H., T. Nakata, Y. Okada, and N. Hirokawa. 1995. KIF3A/B: a heterodimeric kinesin superfamily protein that works as a microtubule plus
end-directed motor for membrane organelle transport. J. Cell Biol. 130:
1387–1399.
Yamazaki, H., T. Nakata, Y. Okada, and N. Hirokawa. 1996. Cloning and characterization of KAP3: a novel kinesin superfamily-associated protein of
KIF3A/3B. Proc. Natl. Acad. Sci. USA. 93:8443–8448.
Yang, Z., and L.S.B. Goldstein. 1998. Characterization of the KIF3C neural kinesin-like motor from mouse. Mol. Biol. Cell. 9:249–261.
Le Bot et al. Role of Xklp3 in Trafficking between the ER and the Golgi
1573
Downloaded from http://rupress.org/jcb/article-pdf/143/6/1559/1851342/9808066.pdf by guest on 31 July 2023
Boleti, H., E. Karsenti, and I. Vernos. 1996. Xklp2, a novel Xenopus centrosomal kinesin-like protein required for centrosome separation during mitosis.
Cell. 84:49–59.
Burkhardt, J.K., C.J. Echeverri, T. Nilsson, and R.B. Vallee. 1997. Overexpression of the dynamitin (p50) subunit of the dynactin complex disrupts dyneindependent maintenance of membrane organelle distribution. J. Cell Biol.
139:469–484.
Cole, D.G., S.W. Chinn, K.P. Wedaman, K. Hall, T. Vuong, and J.M. Scholey.
1993. Novel heterotrimeric kinesin-related protein purified from sea urchin
eggs. Nature. 366:268–270.
Cole, D.G., D.R. Diener, A.L. Himelblau, P.L. Beech, J.C. Fuster, and J.L.
Rosenbaum. 1998. Chlamydomonas kinesin-II-dependent intraflagellar
transport (IFT): IFT particles contain proteins required for ciliary assembly
in Caenorhabditis elegans sensory neurons. J. Cell Biol. 141:993–1008.
Corthesy-Theulaz, I., A. Pauloin, and S.R. Rfeffer. 1992. Cytoplasmic dynein
participates in the centrosomal localization of the Golgi complex. J. Cell
Biol. 118:1333–1345.
Doms, R.W., G. Russ, and J.W. Yewdell. 1989. Brefeldin A redistributes resident and itinerant Golgi proteins to the endoplasmic reticulum. J. Cell Biol.
109:61–72.
Dorner, C., T. Ciossek, S. Mülle, N.P.H. Møller, A. Ulrich, and R. Lammers.
1998. Characterization of KIF1C, a new kinesin-like protein involved in vesicle transport form the Golgi apparatus to the endoplasmic reticulum. J.
Biol. Chem. 273:20267–20275.
Echard, A., F. Jollivet, O. Martinez, J.J. Lacapere, A. Rousselet, I. JanoueixLerosey, and B. Goud. 1998. Interaction of a Golgi-associated kinesin-like
protein with Rab6. Science. 279:580–585.
Feiguin, F., A. Ferreira, K.S. Kosik, and A. Caceres. 1994. Kinesin-mediated organelle translocation revealed by specific cellular manipulations. J. Cell Biol.
127:1021–1039.
Fullekrüg, J., B. Sonnichsen, U. Schafer, P. Nguyen Van, H.D. Soling, and G.
Mieskes. 1997. Characterization of brefeldin A induced vesicular structures
containing cycling proteins of the intermediate compartment/cis-Golgi network. FEBS (Fed. Eur. Biochem. Soc.) Lett. 404:75–81.
Griffiths, G., M. Ericsson, J. Krijnse-Locker, T. Nilsson, B. Goud, H.D. Soling,
B.L. Tang, S.H. Wong, and W. Hong. 1994. Localization of the Lys, Asp,
Glu, Leu tetrapeptide receptor to the Golgi complex and the intermediate
compartment in mammalian cells. J. Cell Biol. 127:1557–1574.
Harada, A., Y. Takei, Y. Kanai, Y. Tanaka, S. Nonaka, and N. Hirokawa. 1998.
Golgi vesiculation and lysosome dispersion in cells lacking cytoplasmic dynein. J. Cell Biol. 141:51–59.
Harlow, E., and D. Lane. 1988. Antibodies: A Laboratory Manual. Cold Spring
Harbor Laboratory Press, Cold Spring Harbor, New York.
Henson, J.H., D.G. Cole, M. Terasaki, D. Rashid, and J.M. Scholey. 1995. Immunolocalization of the heterotrimeric kinesin-related protein KRP(85/95)
in the mitotic apparatus of sea urchin embryos. Dev. Biol. 171:182–194.
Kondo, S., R. Sato-Yoshitake, Y. Noda, H. Aizawa, T. Nakata, Y. Matsuura,
and N. Hirokawa. 1994. KIF3A is a new microtubule-based anterograde motor in the nerve axon. J. Cell Biol. 125:1095–1107.
Kozminski, K.G., P.L. Beech, and J.L. Rosenbaum. 1995. The Chlamydomonas
kinesin-like protein FLA10 is involved in motility associated with the flagellar membrane. J. Cell Biol. 131:1517–1527.
Kreis, T.E., and H.F. Lodish. 1986. Oligomerization is essential for transport of
vesicular stomatitis viral glycoprotein to the cell surface. Cell. 46:929–937.
Lewis, M.J., and H.R. Pelham. 1992. Ligand-induced redistribution of a human
KDEL receptor from the Golgi complex to the endoplasmic reticulum. Cell.
68:353–364.
Liou, W., H. Geuze, and J.W. Slot. 1996. Improving structural integrity of cryosections for immunogold labeling. Histochem. Cell Biol. 106:41–58.
Lippincott-Schwartz, J. 1998. Cytoskeletal proteins and Golgi dynamics. Curr.
Opin. Cell Biol. 10:52–59.
Lippincott-Schwartz, J., N. Cole, and J. Presley. 1998. Unravelling Golgi membrane traffic with green fluorescent protein chimeras. Trends Cell Biol. 8:16–
20.
Lippincott-Schwartz, J., N.B. Cole, A. Marotta, P.A. Conrad, and G.S. Bloom.
1995. Kinesin is the motor for microtubule-mediated Golgi-to-ER membrane traffic. J. Cell Biol. 128:293–306.
Lupas, A., M. Van Dyke, and J. Stoke. 1991. Predicting coiled coils from protein sequences. Science. 252:1162–1164.
Miller, L., and J.C. Daniel. 1977. Comparison of in vivo and in vitro ribosomal
RNA synthesis in nucleolar mutants of Xenopus laevis. In Vitro. 13:557–567.
Morris, R.L., and J.M. Scholey. 1997. Heterotrimeric kinesin II is required for
the assembly of motile 912 ciliary axonemes on sea urchin embryos. J. Cell
Biol. 138:1009–1022.
Murray, A. 1991. Cell cycle extracts. In Xenopus laevis: Practical Uses in Cell
and Molecular Biology. Vol. 36. B.K. Kay and H.B. Peng, editors. Academic
Press, San Diego, CA. 581–605.
Nilsson, T., M. Jackson, and P.A. Peterson. 1989. Short cytoplasmic sequences
serve as retention signals for transmembrane proteins in the endoplasmic
reticulum. Cell. 58:707–718.
Pavelka, M., and A. Ellinger. 1985. Localization of binding sites for concanavalin A, Ricinus communis I and Helix pomatia lectin in the Golgi apparatus of
rat small intestinal absorptive cells. J. Histochem. Cytochem. 33:905–914.
Pazour, G.J., C.G. Wilkerson, and G.B. Witman. 1998. A dynein light chain is
essential for the retrograde particle movement of intraflagellar transport