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Journal of Clinical Microbiology
Evaluation of a Novel Heminested PCR Assay Based on the Phosphoglucosamine Mutase Gene for Detection of <i>Helicobacter pylori</i> in Saliva and Dental Plaque2002 •
Journal of Clinical Microbiology
Evaluation of a Novel Heminested PCR Assay Based on the Phosphoglucosamine Mutase Gene for Detection of Helicobacter pylori in Saliva and Dental Plaque2002 •
A novel heminested PCR protocol was developed for the specific detection of Helicobacter pylori at low copy numbers. A set of primers specific for the phosphoglucosamine mutase gene ( glmM ) of H. pylori produced a 765-bp fragment that was used as template for the heminested primer pair delineating a 496-bp fragment. By using agarose gel electrophoresis for detection of the heminested PCR-amplified products, amplification of H. pylori genomic DNA was achieved at concentrations as low as 0.1 pg, equivalent to 5 × 10 2 bacteria. A study was subsequently undertaken to evaluate the heminested PCR for detection of H. pylori in dental plaque and saliva. Specimens collected from 58 individuals were cultured, and PCR was subsequently performed on the oral cultures. Identification of H. pylori in the same series of saliva and dental plaque specimens was carried out with PCR using a primer pair specific for the H. pylori urease B gene and by the heminested PCR assay. The identity of the ampli...
PubMed
An overview of challenges to eradication of Helicobacter pylori infection and future prospects2017 •
The eradication of H. pylori infection continues to be a challenge due to the evolution of drug-resistant bacteria, lack of a gold standard diagnostic method, and ineffectiveness of current vaccines. Additionally, there still is no consensus in the literature about the main source of gastric H. pylori infection. The bacterium has also been demonstrated to colonize in dental plaque and the oral cavity. We believe that to develop new approaches for successful eradication of the disease, factors such as the biology of the bacterium, reservoir differentiations, host-bacterium interactions and problems in diagnosis, treatment and vaccination must be comprehensively considered. Therefore, the main goal of this study is to gather all of the literature analysis about the problems in the eradication of the infection, reconsider contradictions about extra-gastric reservoirs of the bacterium, and propose new strategies aimed at disease eradication.
Critical Reviews in Microbiology
Colonization of Helicobacter pylori in the oral cavity – an endless controversy?2021 •
Journal of Clinical Microbiology
Incidence of Helicobacter felis and the Effect of Coinfection with Helicobacter pylori on the Gastric Mucosa in the African Population2006 •
Helicobacter pylori and Helicobacter felis are two of the Helicobacter spp. that infect humans. H. pylori has been linked to significant gastric pathology. Coinfection with Helicobacter spp. may influence infectious burden, pathogenesis, and antibiotic resistance; however, this has not been studied. The aims of this study were to identify the incidence of H. felis and to analyze the effects of coinfection with both organisms on gastric pathology in a well-characterized South African population. Biopsy samples from the gastric corpora and antra of volunteers ( n = 90) were subjected to histological examination and PCR for the identification of H. pylori and H. felis . We further investigated the effect of global strain type on the occurrence of precursor lesions by assigning nucleotide sequences derived from PCR amplification of three genes to global groupings (ancestral Africa1, ancestral Africa2, ancestral Europe, ancestral Asia, and mixed). H. pylori was detected in 75 (83.3%), H....
Journal of Clinical Microbiology
Unreliability of Results of PCR Detection of Helicobacter pylori in Clinical or Environmental Samples2009 •
2000 •
World Journal of Gastrointestinal Pharmacology and Therapeutics
Helicobacter pyloriand its reservoirs: A correlation with the gastric infection2016 •
Postępy Higieny i Medycyny Doświadczalnej
Role of PCR in Helicobacter pylori diagnostics and research – new approaches for study of coccoid and spiral forms of the bacteria2013 •
Gastroenterology and hepatology from bed to bench
The role of adenotonsillar tissues as a reservoir for Helicobacter pylori and Helicobacter hepaticus2011 •
World journal of gastroenterology : WJG
Anand PS, Kamath KP, Anil S. Role of dental plaque, saliva and periodontal disease in Helicobacter pylori infection. World journal of gastroenterology : WJG 2014; 20(19): 5639-5653 [PMID: 24914323 PMCID: 4024772 DOI: 10.3748/wjg.v20.i19.5639]2014 •
2006 •
World journal of gastroenterology : WJG
Helicobacter pylori and oral pathology: relationship with the gastric infection2014 •
2021 •
Memórias do Instituto Oswaldo Cruz
Helicobacter pylori transiently in the mouth may participate in the transmission of infection2010 •
Cumhuriyet Dental Journal
Analysing Subgingival Plaque with regard to H.pylori at Chronic and Aggressive Periodontitis Patients2018 •
International Journal of Food Microbiology
Transmission pathway of Helicobacter pylori: Does food play a role in rural and urban areas?2010 •
BMC oral health
The investigation of Helicobacter pylori in the dental biofilm and saliva samples of children with dyspeptic complaints2017 •
Infectious Agents and Cancer
Detection of helicobacter pylori in benign laryngeal lesions by polymerase chain reaction: a cross sectional study2012 •
BMC Gastroenterology
Study of Helicobacter pylori genotype status in cows, sheep, goats and human beings2014 •
World Journal of Gastroenterology
Helicobacter pylori in dental plaque and stomach of patients from Northern Brazil2010 •
Peptic Ulcer Disease
Helicobacter pylori – Not Only a Gastric Pathogene?2011 •
Comparative Clinical Pathology
PCR-based diagnosis of Helicobacter species in the gastric and oral samples of stray dogs2012 •
Digestive Diseases and Sciences
A newly developed PCR assay of H. pylori in gastric biopsy, saliva, and feces1996 •
2012 •
2008 •
2019 •
2004 •
Brazilian Archives of Biology and Technology
Antibacterial Effects of Cinnamon Extract, Clove Oil and Antibiotics against Helicobacter pylori Isolated from Stomach BiopsiesMediterranean Journal of Infection Microbes and Antimicrobials
Association between Helicobacter pylori and Pharyngolaryngeal Carcinomas: The Role in the Development and Prognostic Significance2006 International Conference on Power Electronic, Drives and Energy Systems
A Doubly Fed Induction Motor as High Torque Low Speed Drive2006 •
Journal of medical microbiology
Prevalence of helicobacter pylori at oral and gastrointestinal sites in children: evidence for possible oral-to-oral transmission2002 •
2004 •
Oral microbiology …
Helicobacter pylori in the oral cavity is associated with gastroesophageal disease2009 •