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119 SEX DIFFERENCE IN EXPRESSION PROFILES OF ACIDSENSING ION CHANNELS AND TRANSIENT RECEPTOR POTENTIAL CHANNEL V1 IN THE MOUSE URINARY BLADDER .RED\DVKL+<RVKL\DPD0$UDNL,.LUD6,QX]XND+1DNDJRPL+ 0L\DPRWR7+DQHGD<6DZDGD1.DPL\DPD0.XGRX6=DNRML+ 7VXFKLGD7)XNDVDZD07DNHGD0 University of Yamanashi, Dept. of Urology, Tyuo-City, Japan Introduction & Objectives: ,Q EODGGHULQQHUYDWLQJ VHQVRU\ QHXURQV SURWRQ HYRNHGFXUUHQWVDUHPHGLDWHGODUJHO\E\DFLGVHQVLQJLRQFKDQQHO $6,& LQDGGLWLRQ WR WUDQVLHQW UHFHSWRU SRWHQWLDO FKDQQHO 9 7539  $6,&V DUH HQFRGHG E\ IRXU GLᚎHUHQWVXEXQLWJHQHV$6,&$6,&$6,&DQG$6,&DQGWKHVXEXQLWVIRUP KRPRRUKHWHURPXOWLPHULFFKDQQHOV,QFHQWUDODQGSHULSKHUDOQHUYRXVV\VWHPV $6,&VKDYHHPHUJHGDVNH\UHFHSWRUVIRUH[WUDFHOOXODUSURWRQVDQGUHFHQWVWXGLHV KDYHVXJJHVWHGGLYHUVHUROHVIRUWKHVHFKDQQHOVLQWKHSDWKRSK\VLRORJ\RIDFLG HYRNHGSDLQ,WKDVEHHQUHSRUWHGWKDWWKHIHPDOHEODGGHULVPRUHVHQVLWLYHDQG YXOQHUDEOHWRDFLGLFLUULWDWLRQWKDQPDOHEODGGHU7KLVUHVXOWKDVVWLPXODWHGXVWR IXUWKHU LQYHVWLJDWH WKH XQGHUO\LQJ PHFKDQLVPV IRU WKH VH[ GLᚎHUHQFH 7KXV WKH SUHVHQW VWXG\ ZDV FRQGXFWHG WR H[DPLQH ZKHWKHU WKHUH ZDV D VH[ GLᚎHUHQFH LQ JHQH H[SUHVVLRQV RI $6,& VXEXQLWV DQG WUDQVLHQW UHFHSWRU SRWHQWLDO FKDQQHOV LQ WKHPRXVHXULQDU\EODGGHU Material & Methods: 7KHXULQDU\EODGGHUVRIPDOHDQGIHPDOHPLFHZHUHKDUYHVWHG WRH[DPLQHH[SUHVVLRQVRIWZRDFLGVHQVLWLYHFKDQQHOJHQHV$6,&VDQG7539 XVLQJUHDOWLPH573&5:HDOVRH[DPLQHGJHQHH[SUHVVLRQVRIRWKHUFDQGLGDWHV IRUQRFLFHSWLYHLRQFKDQQHOLH753$DQG7530LQEODGGHUVRIPDOHDQGIHPDOH PLFH )XUWKHU LQYHVWLJDWLRQ XVLQJ WKH VHSDUDWHG PXFRVD DQG PXVFOH OD\HU ZDV SHUIRUPHGIRU$6,&VXEXQLWVWKDWZHUHGRPLQDQWLQWKHJHQHH[SUHVVLRQFRPSDUHG WRWKHRWKHUV$OOYDOXHVDUHH[SUHVVHGDVPHDQs6(06WDWLVWLFDODQDO\VLVZDV PDGHXVLQJ6WXGHQWಬVWWHVW 3 3 3QVQRWVLJQLᚏFDQW  MXQFWLRQV $-ಬV LQWKHXURWKHOLXPRIWKHKXPDQEODGGHU$-ಬVKDYHDQLQWUDFHOOXODU DQGDQH[WUDFHOOXODUGRPDLQ7KHᚏUVWLVFRQQHFWHGWRWKHDFWLQF\WRVNHOHWRQ7KH VHFRQGDQFKRUVQHLJKERXULQJXURWKHOLDOFHOOVWRHDFKRWKHU7RJHWKHUWKLVIRUPVD ULJLGVWUXFWXUDOQHWZRUNWKDWLVDEOHWRWUDQVPLWWKHPHFKDQLFDOIRUFHVWKDWRULJLQDWH GXULQJEODGGHUᚏOOLQJDQGLVWKHUHIRUHDJRRGORFDWLRQWRPHDVXUHVWUHWFK7KHDLP RI RXU VWXG\ ZDV WR IXUWKHU H[SORUH WKH FRORFDOL]DWLRQ RI 7539  $-ಬV LQ WKH XURWKHOLXPRIWKHKXPDQXURJHQLWDOWUDFW NLGQH\XUHWHUEODGGHU 6HFRQGDULO\ZH ZDQWHGWRFRQᚏUPWKLVFRORFDOL]DWLRQLQDQRWKHUVSHFLHV PRXVH  Material & Methods: :H XVHG WLVVXH IURP KHDOWK\ VHFWLRQV RI F\VWHFWRP\  QHSKUHFWRP\ SDWLHQWV %ODGGHUV DQG NLGQH\V REWDLQHG IURP QRUPDO  7539  PLFH ZHUH XVHG DV SRVLWLYH DQG QHJDWLYH FRQWUROV 7LVVXH ZDV SUHSDUHG IRU LPPXQRKLVWRFKHPLVWU\ $IWHU LQFXEDWLRQ ZLWK SULPDU\ DQWLERGLHV IRU 7539  (FDGKHULQH DQG VHFRQGDU\ DQWLERGLHV $OH[DಬV      '$3, VDPSOHV ZHUH HYDOXDWHG ZLWK HSLIXRUHVFHQW PLFURVFRSH :HVWHUQ EORWWLQJ DQG LPPXQRSUHFLSLWDWLRQVZHUHGRQHRQKXPDQPRXVHDQG7539PRXVHNLGQH\V %HVLGHV WKH SUHYLRXV DQWLERGLHV DOVR DQWLERGLHV IRU ˞FDWHQLQ  ˟FDWHQLQ ZHUH XVHG Results: 7539 ZDVGHWHFWHG LQKXPDQ PRXVH XURWKHOLXP RIEODGGHU XUHWHU NLGQH\ RQO\LQGLVWDOWXEXOL FROOHFWLQJGXFWV DQGFRORFDOL]HGZLWKDGKHUHQFH MXQFWLRQV LQ DOO WLVVXHV H[DPLQHG ,PPXQRSUHFLSLWDWLRQ H[SHULPHQWV UHYHDOHG WKDW 7539 LV ERXQG WR ˞FDWHQLQ 7KLV FRQQHFWLRQ HQDEOHV 7539 WR PHDVXUH VWUHWFK Conclusions: 7539 LV FRQQHFWHG WR WKH LQWUDFHOOXODU GRPDLQ RI $-ಬV LQ WKH XURWKHOLXPRIWKHEODGGHUWKHXUHWHU WKHGLVWDOWXEXOLRIWKHNLGQH\7KHVHᚏQGLQJV VXJJHVWWKDW7539LVLQYROYHGLQWKHVHQVDWLRQRIVWUHWFKLQWKHXURWKHOLXP Results: ,QWKHZKROHEODGGHU$6,&DQG$6,&VXEXQLWJHQHVZHUHDEXQGDQW ZKHUHDV $6,& ZDV VFDQW\ DQG $6,& ZDV YLUWXDOO\ QRW GHWHFWHG )LJ   6H[ GLᚎHUHQFHLQJHQHH[SUHVVLRQOHYHOZDVIRXQGRQO\LQ$6,& )LJ $6,&ZDV SUHGRPLQDQW $6,& VXEXQLW H[SUHVVHG LQ WKH PXFRVD ZKHUHDV ERWK $6,& DQG $6,&ZHUHODUJHO\H[SUHVVHGLQWKHPXVFOHOD\HU )LJ ,QWKHPXFRVD$6,& ZDVPRUHKLJKO\H[SUHVVHGLQPDOHWKDQLQIHPDOHZKHUHDVWKHH[SUHVVLRQOHYHORI $6,&LQWKHPXVFOHOD\HUZDVKLJKHULQIHPDOHWKDQLQPDOHPLFH )LJ 7KHVH[ GLᚎHUHQFHZDVIRXQGLQWKH7539JHQHH[SUHVVLRQOHYHODOEHLWWKHTXDQWLWDWLYH H[SUHVVLRQVRI7539753$DQG7530ZHUHYHU\SRRU )LJVDQG  Conclusions: 7KH SUHVHQW UHVXOWV VXJJHVW WKH SRVVLELOLWLHV WKDW $6,&V PD\ EH LQYROYHG LQ DᚎHUHQWVLJQDOWUDQVGXFWLRQDQGGHWUXVRUFRQWUDFWLOLW\DQGWKDW $6,&V DQG7539SDUWLFLSDWHLQVH[GLᚎHUHQFHLQDFWLYLW\RIORZHUXULQDU\WUDFWLUULWDWHGE\ DFLG>@,QYLYRIXQFWLRQDOVWXG\LVIXUWKHUQHFHVVDU\WRGHWHUPLQHLIWKHVHFKDQQHOV DUHLQYROYHGLQPHFKDQLVPVJHQHUDWLQJLUULWDWLYHEODGGHUV\PSWRPV 120 TRPV4 IN THE UROTHELIUM OF THE UROGENITAL TRACT. A CO-LOCALIZATION BETWEEN TRPV4 AND ADHERENCE JUNCTIONS IN HUMAN KIDNEY, URETER & URINARY BLADDER SUGGEST INVOLVEMENT OF TRPV4 IN STRETCH SENSATION -DQVVHQ'$:+HHVDNNHUV-3)$-DQVHQ&)-6FKDONHQ-$ Radboud University Nijmegen Medical Centre, Dept. of Urology, Nijmegen, The Netherlands Introduction & Objectives: :HLQYHVWLJDWHGWKHORFDWLRQRI7539LQWKHXURWKHOLXP RIWKHNLGQH\XUHWHU EODGGHU7539LVDFDWLRQFKDQQHOWKDWLVORFDWHGRQWKH FHOOPHPEUDQHV RI WKH EODGGHU XURWKHOLXP $FFXPXODWLQJ HYLGHQFH SURYH WKDW 7539FKDQQHOVIXQFWLRQDVPHFKDQRUHFHSWRUV7KLVKDVOHGWRWKHK\SRWKHVLVWKDW 7539LVLQYROYHGLQEODGGHUᚏOOLQJVHQVDWLRQ,WFRXOGDOVREHLQYROYHGLQGLVHDVHV WKDW FDXVH YRLGLQJ G\VIXQFWLRQ 2$%3%6  ,Q UHFHQW LPPXQRKLVWRFKHPLVWU\ H[SHULPHQWV ZH KDYH SURYHQ D FRORFDOL]DWLRQ EHWZHHQ 7539 DQG DGKHUHQFH Eur Urol Suppl 2010;9(2):70 121 THE EXPRESSION OF TRANSIENT RECEPTOR POTENTIAL (TRP) V4, A1, AND V1 IN THE HUMAN BLADDER MUCOSA OF NORMAL AND BLADDER OUTLET OBSTRUCTION: A NOVEL MECHANISM IN THE OBSTRUCTION-INDUCED BLADDER OVERACTIVITY- 1DNDJRPL+.LUD60L\DPRWR7,QX]XND+6DZDGD6.DPL\DPD0 .RED\DVKL+.XGR60RFKL]XNL77DNHGD0 University of Yamanashi, Dept. of Urology, Chuo, Japan Introduction & Objectives: 7UDQVLHQWUHFHSWRUSRWHQWLDO 753 LRQFKDQQHOIDPLO\ KDVXQLTXHFKDUDFWHUVRIWKHUPRVHQVLQJDQGPHFKDQRVHQVLQJDQGVRPHRI753V PD\ EH FDQGLGDWH IRU PHFKDQRVHQVRU LQ WKH XULQDU\ WUDFW 7KH REMHFWLYHV RI WKLV VWXG\ DUH WR LQYHVWLJDWH WKH H[SUHVVLRQ SURᚏOHV RI 753$ 7539 DQG 7539 LQ QRUPDO DQG REVWUXFWHG %22  KXPDQ EODGGHU PXFRVD DQG WR FRPSDUH WKH H[SUHVVLRQSURᚏOHVRI753VLQEODGGHUPXFRVDDQGFOLQLFDOSDUDPHWHUVLQFOXGLQJ ORZHUXULQDU\WUDFWV\PSWRPV Material & Methods: 8ULQDU\EODGGHUPXFRVDOVDPSOHVIURPSDWLHQWV s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ಬVUDQNVXPWHVW Results: (YHU\P51$DQGSURWHLQRI753$7539DQG7539ZHUHGHWHFWHG LQ HYHU\ VDPSOH RI ERWK %22 DQG QRQ%22 SDWLHQWV DQG 753$ VKRZHG KLJKHVW H[SUHVVLRQ RI P51$ DPRQJ  753V 7KH H[SUHVVLRQ RI P51$ ZHUH KLJKHU LQ %22 WKDQ QRQ%22 LQ HYHU\ 753 P51$ H[SUHVVLRQ RI 753$ ZHUH s DQG s LQ %22 DQG QRQ%22 UHVSHFWLYHO\ 3  P51$H[SUHVVLRQRI7539ZHUHsDQGsLQ%22DQGQRQ