Nucleic Acids Research Volume 17 Number 4 1989 A wheat cDNA clone which is homologous to the 17 kd heat-shock protein gene family of soybean Elizabeth F.McElwain and Steven Spiker Department of Genetics, North Carolina State University, Raleigh, NC 27695, USA Submitted January 18, 1989 EMBL accession no. X13431 We screened a wheat cDNA library with antibodies to low molecular weight proteins from wheat germ. Three of the clones selected are homologous to the family of 17 kd heatshock protein (hsp) genes of soybean (1). The wheat C5-8 clone has an open reading frame encoding a 150 amino acid polypeptide that is identical to the 17 kd soybean hsps at nearly 70% of the amino acid residues. The wheat C5-8 and soybean 17.5E open reading frames are aligned below (dashes indicate identities, asterisks mark gaps). The amino acid sequences differ significantly at the N-termini, but the C-terminal two-thirds of the proteins are 83% identical (74% at the nucleotide level). This is the first gene isolated from a monocot that has homology to the soybean hsp genes. 40 10 20 30 50 60 70 90 100 80 110 GAATTCCCTTTTCCTACCTACGATCCGATACCGAATTTTCCGAGCGCACAAGCCAAACCAAAGCAAACACTGACGATGTCGATCGTGAGGCGGAGCAACGTGTTCGACCC MS I V R R S N V F D P Uheat C5-8: Gnhsp 17.5E: 1 S L I P G F F G G - - S - - - - 180 190 200 140 150 160 170 210 120 130 220 CTTCGCCGACCTTTGGGCGGACCCCTTCGACACCTTCCGTTCCATCGTCCCGGCGATCTCAGGCGGCAGCAGCGAGACGGCCGCGTTCGCCAACGCCCGTGTGGACTGGA F A D L W A D P F D T F R S I V P A I S G G S S E T A A F A N A R V D WI K -S L D M W - - - K D - H V P T S S V - A E N -*** - - V S T - - - - 230 240 250 260 270 280 290 300 310 320 330 AGGAGACCCCCGAGGCGCACGTCTTCAAGGTCGACCTCCCCGGCGTGAAGAGGAGGAGGTCAAGGTGGAGGTGGAGGACGGCAACGTGCTCGTCGTCAGCGGCGAGCGG E T P E A H V F K V D L P G V K K E E V K V E V E D G N V L V V S G E R - -- - -A - I - - L - - - - - - - Q I -- D R -- Q I - - - 340 350 360 370 380 390 400 410 420 430 440 AGCAGGGAGAAGGAGGACAAGAACGACAAGTGGCACCGCGTGGAGCGCAGCAGCGGCAAGTTCGTGCGGCGTTTCCGGCTGCCGGAGGACGCCAAGGTGGAGGAGGTCAA S R E K E D K 11 D K W H R V E R S S G K F V R R F R L P E D A K V E E V K N V - - - - - - - T - - - - - - - - - - - T - - - - - - - 11 - - - N1 - - 450 460 480 470 500 490 510 520 530 540 550 GGCCGGGCTGGAGAACGGCGTGCTCACCGTCACCGTGCCCAAGGCCGAGGTCAAGAAGCCCGAGGTGAAGGCCATCGAGATCTCCGGCTGACTGCATGCGTTTGGACGTA A G L E N G V L T v T v P K A E v K K P E v K A I E I s G -S N 560 - - - 570 - - - 580 - - - 590 - E - - 600 - - - 610 D - - 620 - - - - 630 - - 640 650 660 TGCTCATTCAGGTTGGAGCCAATTTGGTTGATGTGTGTGCGAGTTCTTGCGAGTCTGATGAGACATCTCTGTATTGTGTTTCTTTCCCCAGTGTTTTCTGTACTTGTGTA 670 680 690 700 710 720 730 740 ATCGGCTAATCGCCAACAGATTCGGCGATGAATAATGAGAAATAAATTGTTCTGATTTTGAGTGMMMGGAATT ACKNOWLEDGEMENTS This research was supported by NIH grant GM-37810. This is paper 11988 of the Journal Series of the North Carolina Agricultural Research Service, Raleigh, NC. 27695-7643 REFERENCE 1. Czarnecka, E. et al. (1985) Proc. Natl. Acad. Sci. USA 82:3726-3730. 1764 IRL Press <) I RL Press