Academia.edu no longer supports Internet Explorer.
To browse Academia.edu and the wider internet faster and more securely, please take a few seconds to upgrade your browser.
2014, Cancer Research
Circadian deregulation is associated with the development of numerous diseases, including cancer, diabetes, and several neurological disorders. A recent study shows that the onset of carcinogenicity is specifically linked to the time of exposures (day or night) to ultraviolet light and ionizing radiation in mice. This effect is believed to be mediated through radiation-induced DNA damage and deregulation of the WNT/β-Catenin signaling pathway, hence affecting cell survival and proliferation. Results: The current studies aim to elucidate the contribution of a core circadian gene, Period2 (Per2), in the radioprotective response of mice bone marrow. Epithelial cells and mice bone marrow express PER2 in contrast to cancer cells. Bone marrow isolated from Per2 knockout mice exhibit radioresistence to high doses of ionizing radiation (HDIR; 2Gy) compared to wild-type mice controls suggesting that Per2 expression was responsible for radiation-induced cell death. This finding was translated by increased granulocyte-macrophage (GM-CFUs) clonogenic survival and reduced cell apoptosis. Mechanistic analysis showed that Per2 knockout mice bone marrow expressed higher levels of procaspase3 and less expression of active caspase3 after HDIR treatment compared to wild-type mice bone marrow and sham irradiated controls. Furthermore, Per2 knockout mice treated with 12Gy total body radiation (TBIR) showed an 80% survival verses 30% survival in wild-type mice. Whole genome-wide sequencing detected signal transduction pathways reflecting enhanced DNA damage repair capacity in knockout mice bone marrow, hence defining the role of PER2 in the radioresistant phenotype in vivo. Conclusions: This data is the first evidence supporting the critical role of PER2 in protecting mice hematopoietic stem cells (HSCs) from HDIR via enhancement of genes involved in DNA repair signaling networks.
Stem Cell Reports
A Single Radioprotective Dose of Prostaglandin E2 Blocks Irradiation-Induced Apoptotic Signaling and Early Cycling of Hematopoietic Stem Cells2014 •
Antioxidants
Radiation Type- and Dose-Specific Transcriptional Responses across Healthy and Diseased Mammalian TissuesIonizing radiation (IR) is a genuine genotoxic agent and a major modality in cancer treatment. IR disrupts DNA sequences and exerts mutagenic and/or cytotoxic properties that not only alter critical cellular functions but also impact tissues proximal and distal to the irradiated site. Unveiling the molecular events governing the diverse effects of IR at the cellular and organismal levels is relevant for both radiotherapy and radiation protection. Herein, we address changes in the expression of mammalian genes induced after the exposure of a wide range of tissues to various radiation types with distinct biophysical characteristics. First, we constructed a publicly available database, termed RadBioBase, which will be updated at regular intervals. RadBioBase includes comprehensive transcriptomes of mammalian cells across healthy and diseased tissues that respond to a range of radiation types and doses. Pertinent information was derived from a hybrid analysis based on stringent literatu...
2015 •
Cell Biology and Toxicology
Analysis of DNA-damage response to ionizing radiation in serum-shock synchronized human fibroblastsInternational journal of immunopathology and pharmacology
Peroxiredoxin genes are not induced in myeloid leukemia cells exposed to ionizing radiationPeroxiredoxins (Prx) comprise an extended family of small antioxidant proteins which conserve a thioredoxin-dependent catalytic function that can contribute to cell protection from reactive oxygen species (ROS). ROS generation is one of the deleterious intracellular effects of ionizing radiation, but the role of Prx during radiation treatment has not been extensively explored. Present experiments measure effects of ionizing radiation on expression of human Prx types I (PAGA), II (NKEF-B) and IV (AOE372) in human myeloid leukemia cells (K562). Prx gene transcription was analyzed by amplifying with RT-PCR cDNAs complementary to each Prx-specific coding sequence and by identifying the derived products with Southern blotting procedure. Transcripts of GAPDH were used as the endogenous standard for semi-quantitative comparisons. No consistent increase in Prx gene expression was detected at time intervals up to 72 h after gamma radiation doses that caused cell cycle arrest and nuclear dama...
European Journal of Pharmacology
New molecular targets in radiotherapy: DNA damage signalling and repair in targeted and non-targeted cells2009 •
Revista Ensaios Teológicos
Charles Haddon Spurgeon: O Príncipe Dos Pregadores2020 •
Normas de Información Financiera
Normas de información financiera2019 •
2023 •
2023 •
Environmental science and pollution research international
Chemically and size-resolved particulate matter dry deposition on stone and surrogate surfaces inside and outside the low emission zone of Milan: application of a newly developed "Deposition Box2018 •
Journal of Food Processing and Preservation
Radiant heat treatments for corn drying and decontamination2017 •
Jurnal Kesehatan Andalas
Identifikasi Bakteri Coliform yang Terdapat pada Minuman Es Teh di Rumah Makan Tepi Laut Purus Padang Barat2015 •
Environmental Science & Technology
In Situ Bioreduction of Technetium and Uranium in a Nitrate-Contaminated Aquifer2004 •