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Accepted Article Optical Coherence Tomography in Evaluation of Glomus Tumors: A Report of Three Cases Running Head: OCT in Evaluation of Glomus Tumors Ali Rajabi-Estarabadi, MD1; Ariel E. Eber, MD1; John Tsatalis, BS1; Thomas Vasquez, BS2; Marina Perper, BS1; Keyvan Nouri, MD1; Antonella Tosti, MD1 1 Dr. Phillip Frost Department of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, FL, USA 2 Florida International University Wertheim College of Medicine, Miami, FL, USA Corresponding Author: Antonella Tosti, MD Address: 1295 NW 14th St. University of Miami Hospital South Bldg. Suites K-M, Miami, FL 33125 Tel: +1(305) 498 0598 Email: atosti@med.miami.edu Funding: None Disclosures: None declared Glomus tumors are painful, benign perivascular neoplasms of the glomus body affecting the nail apparatus. Although diagnosis can be made clinically using Love’s and Hildreth’s tests, imaging is required to distinguish glomus tumors from other subungual vascular lesions. Clinical diagnosis is complicated when the nail has no visible abnormalities.1 Magnetic resonance imaging (MRI) is the modality of choice for imaging but requires a dedicated small part coil, which is unavailable at some facilities and is costly.2 This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1111/jdv.15626 This article is protected by copyright. All rights reserved. Accepted Article Demand for non-invasive skin imaging makes dynamic optical coherence tomography (D-OCT) an interesting option in dermatology. D-OCT is a non-invasive, real-time imaging modality offering in vivo diagnostic capability using infrared broadband light to image superficial skin layers with high resolution and moderate depth penetration, and to visualize blood vessel flow.3-5 D-OCT demonstrated potential for vascular and structural feature identification unique to nail psoriasis, and in glomus tumors has potential as a cheaper, faster MRI alternative.6 Case 1 A 36-year-old woman presented with history of a painful subungual glomus tumor of the right thumb excised 3 years prior. She complained of moderate pain and tenderness. The nail plate showed blanching erythema on the lunula that could be either due to post-surgical change or tumor recurrence. D-OCT was conducted using VivoSight ‘Rx’ dermatological OCT scanner equipped with ‘Dynamic OCT' processing and software (Michelson Diagnostics, Kent). Vessel architecture and blood flow measurements at 0.05 mm depth intervals revealed morphological changes of the nail, including lunula destruction by mass effect and longitudinal ridging (Figure 1a). Dynamic blood flow underscored presence of a vascular tumor, with statistically significant increased flow pronounced above 0.8 mm (Figure 1b). MRI confirmed diagnosis, visualizing an ovoid mass, dorsal cortex erosion of the distal phalanx, and T2 hypersensitivity.7,8 Post-surgical pathology confirmed a recurrent subungual glomus tumor. Case 2 A 65-year-old male presented with a cold-sensitive nail. The persistent symptoms began ten years prior after right fourth finger trauma. Examination revealed a purple/brown tender subungual area and onycholysis distal to the lesion. D-OCT revealed an ovoid area of hypointensity with increased peripheral vascularity (Figure 2a). Sagittal OCT showed onycholysis. MRI visualized a well-circumscribed nail bed mass with increased T2 and intermediate T1 signal intensity consistent with glomus tumor. Diagnosis was confirmed by pathology. Case 3 A 52-year-old female presented with a subungual lesion that appeared 3 years prior and was moderately painful upon palpation. Examination found a tender red papule below the right fourth digit nail plate. MRI was not covered by insurance. This article is protected by copyright. All rights reserved. Accepted Article D-OCT revealed a well-circumscribed area of increased vascularity approximately 0.8 mm below the nail plate (Figure 2b). Coronal OCT revealed structural changes of the nail plate consistent with tumor mass effect. Surgery was performed and histologic examination showed a circumscribed dermal nodule of convoluted vascular channels surrounded by layers of cuboidal cells consistent with glomus tumor. D-OCT is well-suited as an outpatient diagnostic imaging modality for glomus tumors, and may help distinguish angiomatous subtypes from less vascular glomus tumors.9 Cases 1 and 3 are consistent with angiomatous subtype, with Case 1 demonstrating convoluted vascular channels on microscopy. Case 2 demonstrates general hypointensity and minimal tumoral core vascularity likely representing the more solid mass core. D-OCT offers distinct advantages over MRI, which is costly, time-intensive, and requires specialized, equipment. D-OCT poses no risks to patients, is affordable, and should be considered an effective, quick imaging alternative. As the first documented case of OCT for glomus tumor diagnosis, further comparative studies are required to substantiate diagnostic efficacy, but this technique has significant potential. This article is protected by copyright. All rights reserved. Accepted Article References 1. Mohler DG, Lim CK, Martin B. Glomus tumor of the plantar arch: a case report with magnetic resonance imaging findings. Foot Ankle Int. 1997;18(10):672-674. 2. Al-Qattan MM, Al-Namla A, Al-Thunayan A, Al-Subhi F, El-Shayeb AF. Magnetic resonance imaging in the diagnosis of glomus tumours of the hand. J Hand Surg Br. 2005;30(5):535-540. 3. Welzel J. Optical coherence tomography in dermatology: a review. Skin Res Technol. 2001;7(1):1-9. 4. Sattler E, Kastle R, Welzel J. Optical coherence tomography in dermatology. J Biomed Opt. 2013;18(6):061224. 5. Ulrich M, Themstrup L, de Carvalho N, et al. Dynamic Optical Coherence Tomography in Dermatology. Dermatology. 2016;232(3):298-311. 6. Aldahan AS, Chen LL, Fertig RM, et al. Vascular Features of Nail Psoriasis Using Dynamic Optical Coherence Tomography. Skin appendage disorders. 2017;2(3-4):102-108. 7. Glazebrook KN, Laundre BJ, Schiefer TK, Inwards CY. Imaging features of glomus tumors. Skeletal Radiol. 2011;40(7):855-862. 8. Koc O, Kivrak AS, Paksoy Y. Subungual glomus tumour: magnetic resonance imaging findings. Australas Radiol. 2007;51 Spec No.:B107-109. 9. Carroll RE, Berman AT. Glomus tumors of the hand: review of the literature and report on twenty-eight cases. J Bone Joint Surg Am. 1972;54(4):691-703. This article is protected by copyright. All rights reserved. Accepted Article Figures Figure 1. a: Dermatoscopic image of the recurrent glomus tumor on the affected digit. The subungual lesion is visible through the nail plate. b: Blood flow (y-axis) as measured by depth (mm) below the skin surface (x-axis). The series in blue represents the affected thumb while the orange series represents the unaffected digit. Blood flow was measured at 0.05 mm depth intervals. Analysis revealed a statistically significant increase in blood flow, especially above depths of 0.8 mm. These findings are consistent with and reinforce the suspicion that there is a glomus tumor present. Figure 2. a: Dynamic OCT of the nail bed at 0.1 mm intervals demonstrating hypointensity and tumoral borders from depths of 0.4 mm to 0.9 mm. Borders are indicated with yellow arrows (Panel D). Distal onycholysis is also seen from depths of 0.1 mm (not depicted) to 0.4 mm (Panel A). b: Sagittal view of the nail plate showing a well circumscribed area of increased vascularity consistent with a vascular tumor. This article is protected by copyright. All rights reserved. Accepted Article This article is protected by copyright. All rights reserved. Accepted Article This article is protected by copyright. All rights reserved.