Proceedings of the 10th International Conference on Environmental Science and Technology
Kos island, Greece, 5 – 7 September 2007
ADVANCED TREATMENT BY OZONATION AND SONOLYSIS FOR
DOMESTIC WASTEWATER REUSE
H. SELCUK1, V. NADDEO2, L. RIZZO2,* and V. BELGIORNO2
1
Department of Environmental Engineering, Pamukkale University, 20017, Kinikli, Denizli,
Turkey, 2 Depart. of Civil Engineering, University of Salerno, Via Ponte don Melillo, 84084
Fisciano (SA), Italy;
*corresponding author, e-mail: l.rizzo@unisa.it
EXTENDED ABSTRACT
In many countries, especially in arid and semi-arid areas, because of the leak of natural
water resources the wastewater reuse is an important integrative/alternative resource for
not drinking uses (such as urban, agricultural and industrial). Disinfection is one of the
most important step for the reuse of treated domestic wastewater to eliminate infectious
diseases and chlorination is for sure the most used disinfection process. Unfortunately,
the formation of hazardous chlorination by-products restrict the usage of chlorine for the
disinfection of wastewater. In Italy a very stringent limit for chlorination by products such
as trihalomethanes (30 μg/L) was set for wastewater reuse. Accordingly, the use of
alternative oxidation/disinfection systems should be evaluated as possible alternative to
chlorine. Ozone is a powerful disinfectant for the inactivation of a wide range of
microrganisms as well as oxidizing a wide range of organic and inorganic pollutants.
Recently ultrasounds were found to be effective as pre-treatment for wastewater
disinfection by UV irradiation as well as the combined affect of ozone and ultrasounds
provided good efficiency for disinfection of well water. In this context, we investigated the
advanced wastewater treatment by ozonation and ultrasounds in terms of bacteria
inactivation (in particular Escherichia coli) as well as colour removal (as UV absorbance
at 436 nm, UV436). Domestic wastewater samples taken from the effluent of wastewater
treatment plants, were used in ozonation and sonolysis experiments. The disinfection
efficiency by ozone increased from 68.6% to 97.1% as contact time increased from 3 to
15 min respectively. According to the investigated conditions (max ozone dose: 28 mg/(L
min); max contact time: 30 min), the ozonation process alone was not enough to
decrease the E. coli colonies under the limit set for wastewater reuse in Italy (10 CFU/100
mL). Sonolysis improved ozonation process; after 15 min contact time E. coli colonies
decreased under 10 CFU/100 mL. Moreover, ozonation process was effective for colour
removal; the UV436 decreased from 13 to 41% as contact time increased from 3 to 15 min.
On the opposite, because the sonolysis process alone increased colour, not significant
differences in terms of UV436 were observed comparing ozonation and combined
sonolysis/ozonation process.
Keywords: Escherichia coli, colour removal, ozonation, sonolysis, wastewater reuse.
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1.
INTRODUCTION
In many countries, especially in arid and semi-arid areas, because of the leak of natural
water resources the wastewater reuse is an important integrative/alternative resource for
not drinking uses (such as urban, agricultural and industrial). Moreover, in some countries
in order to meet the very stringent limits set for wastewater disposal, the upgrading costs
of wastewater treatment plants (WTPs) are so high that wastewater is too valuable to be
wasted, so the wastewater reuse seems to be an attractive alternative option. On the
other hand, wastewater reuse regulation might be stringent as well. Disinfection method
is one of the most important part for the reuse of treated domestic wastewater to
eliminate infectious diseases. Recently hazardous chlorination by-products restricted the
use of chlorine for the disinfection of wastewater (De Feo et al., 2006). In Italy a very
stringent limit for chlorination by products such as trihalomethanes (30 μg/L) was set for
wastewater reuse. Accordingly, the use of alternative oxidation/disinfection systems
should be evaluated as possible alternative to chlorine.
Ozone is a powerful disinfectant for the inactivation of a wide range of microrganisms as
well as oxidizing a wide range of organic and inorganic pollutants. In order to improve
oxidation/disinfection efficiency and decrease contact time as well as applied doses
(which can increase both the treatment costs and formation of undesirable by products),
ozone was also used together with UV radiation, H2O2, and more recently ultrasounds
(Jyoti and Pandit, 2004).
Ultrasounds produce strong cavitation in aqueous solution resulting in formation of shock
wave (Parlitz, et al. 1995) and reactive free radicals (Hua, et al. 2000) because of the
violent collapse of the cavitation bubbles. These effects should contribute to the physical
disruption of microbial structures (Radel, et al. 2000) and inactivation (Mason, et al. 2003,
Naddeo et al. 2005) as well as the decomposition of toxic chemicals (Joyce, et al. 2003).
The physical-chemical water characteristics significantly contribute to ultrasound
disinfection efficiency (Futura, et. al. 2004), and in any case, the implosion of the
cavitation bubbles can drastically increase the water turbidity (Naddeo et al. 2007).
Recently ultrasounds were found to be effective as pre-treatment for wastewater
disinfection by UV irradiation (Neis and Blume, 2002) as well as the combined affect of
ozone and ultrasounds provided good efficiency for disinfection of well water (Jyoti and
Pandit, 2004).
In this context, we investigated the advanced wastewater treatment by ozonation and
ultrasounds in terms of bacteria (Escherichia coli) inactivation as well as colour removal.
2.
MATERIALS AND METHODS
2.1. Wastewater samples
Domestic wastewater samples were taken from three WTPs located in province of
Salerno (southern Italy). The sewage loading of WTP1, WTP2 and WTP3 comes from
8000, 1000 and 500 people respectively. Due to surrounding tourist area, the loading of
WTP1 and WTP2 doubles in summer season. The wastewater characteristics of the WTP
effluents are shown in table 1.
The wastewater are characterized by high fluctuation in several parameters, such as
BOD5 (WTP1), COD (WTP1, WTP3), Cl- (WTP1), NH4 and E. coli. The wastewater
samples used for ozonation and sonolysis tests were taken downstream of activated
sludge process, just upstream of final disinfection step by chlorine.
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Table 1: wastewater quality characteristics (average ± standard deviation) of the
investigated WTPs
Parameter
Units
WTP1
WTP2
WTP3
pH
7.34 ± 0.22
7.5 ± 0.1
7.7 ± 0.2
TSS
mg/L
11.8 ± 6.3
9.9 ± 6.2
13.6 ± 10.2
BOD5
mg/L
35.4 ± 42
12.7 ± 6.1
20.3 ± 11.4
COD
mg/L
113.6 ± 186
40.7 ± 23.1
90.2 ± 77.8
Free Cl2
mg/L
0.12 ± 0.04
0.1 ± 0.05
0.1 ± 0.06
SO4mg/L
23.5 ± 11.4
39.6 ± 7.9
21.0 ± 7.4
Clmg/L
168 ± 279
99.9 ± 6.9
62,3 ± 11.3
Total P
mg/L
0.82 ± 0.32
1.7 ± 0.7
1.1 ± 0.5
NH4
mg/L
0.84 ± 1.09
3.8 ± 8.5
3.0 ± 4.3
NO2mg/L
0.11 ± 0.15
0.1 ± 0.4
0.1 ± 0.2
NO3mg/L
12.2 ± 5.5
13.8 ± 5.1
10.0 ± 7.5
CFU/100 mL
7682± 10238
500 ± 727
4860 ± 9945
E. coli
2.2. Analysis
Membrane filter method (acetate cellulose type filter with 0.45 µm) was used to count
bacteria according to Standard Methods (AWWA-APHA-WEF, 1998). Bacterial count was
carried out using TBX media (Oxoid) for detection of Escherichia coli (E. coli). The plates
were incubated at 44°C for 24 h. The results were expressed in colony forming units per
100 mL (CFU/100 mL). Colour removal was evaluated by means of UV absorbance
measurements at 436 nm (UV436); the effect of the processes was investigated in terms of
behaviour of UV absorbance spectra too. Absorbance measurements were performed
using a λ12 UV-Vis spectrophotometer from Perkin Elmer. Turbidity was detected by
HACH turbidimeter (model 2100 N). The applied ozone was estimated according to
Standard Methods (AWWA-APHA-WEF, 1998).
2.3. Ozonation tests
Ozonation experiments were carried using an ozone generator (Microlab model by
Aeraque I.T., Italy) characterized by a maximum mass flow rate of 1.5 g O3/h, maximum
gas flow rate of 200 NL/h, and a work pressure of 0.5 bar. The tests were carried out
adjusting ozone dose at 28 mg L-1 min-1. Samples were taken at different contact time to
be analysed for E. coli and absorbance.
2.4. Ultrasound tests
The ultrasound device used was a Sonics VCX-750, equipped with a horn tip of 1.3 cm
diameter which is operated at 20 kHz. Electrical power in the range of 6-50 W was
applied, and power density ranged from 25 to 200 W/L. The tests were carried out with
(TC) and without (NTC) temperature control. The temperature was monitored during the
tests. Samples were taken at different sonication time to be analysed for E. coli and
absorbance.
2.5. Ozonation-ultrasound combined tests
The ozonation-ultrasound combined tests were carried out putting ozone diffuser and
ultrasound probe in the same reactor just in controlled temperature conditions.
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3.
RESULTS AND DISCUSSION
3.1. Escherichia coli inactivation
The disinfection efficiency by ozone increased from 68.6% to 97.1% as contact time
increased from 3 to 15 min respectively (Figure 1).
100
E.coli inactivation (%)
90
80
70
60
50
40
30
20
10
0
3
5
7.5
10
15
ozonation time (min)
Figure 1: E.coli inactivation by ozone.
According to the investigated conditions (max ozone dose: 28 mg/(L min); max contact
time: 30 min) and wastewater characteristics, the ozonation process alone was not
enough to decrease the E. coli colonies under the limit set for wastewater reuse in Italy
(10 CFU/100 mL). The use of ultrasound improved ozonation process after 15 min
contact time (Figure 2); in wastewater sample characterized by 700 CFU/100 mL initial E.
coli density the combined process ozonation/sonolysis allowed to decrease the E. coli
colonies under 10 CFU/100 mL.
100
E. coli inactivation (%)
98
96
94
92
90
88
86
84
82
80
O3
US (TC)
US (NTC)
US+O3 (TC)
Figure 2: E.coli inactivation by ozone, ultrasound (with (TC) and without temperature
control (NTC)), ultrasound and ozone simultaneously.
However, the experiments showed that the increased temperature due to ultrasound
application may significantly effect the disinfection efficiency (Figure 2), so, in order to
better understand the influence of temperature, further ultrasound experiments were
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carried out using different power densities, with and without temperature control. In
Figure 3 the E.coli inactivation results achieved in these sonication tests are plotted; the
initial E. coli density was 150000 CFU/100mL. At 15 min contact time, the influence of
temperature was significant for densities equal or higher than 100 W/L; in this
experimental conditions, the final temperature reached a maximum value of 50°C. At 30
min contact time the temperature effect was significant for all three investigated power
densities (the maximum final temperature was 80°C). Under not controlled temperature
conditions, the total E. coli inactivation at 15 min contact time was achieved using a
power density of 200 W/L. Under controlled temperature conditions the total inactivation
was reached at 30 min using the maximum power density (200 W/L).
100
E.coli inactivation (%)
90
80
70
60
50
40
30
25 W/L (TC)
100 W/L (TC)
200 W/L (TC)
20
10
25 W/L (NTC)
100 W/L (NTC)
200 W/L (NTC)
0
15
30
Sonication time (min)
Figure 3: E.coli inactivation by ultrasound with (TC) and without temperature control
(NTC), using different ultrasound densities, according to contact time.
3.2. Colour removal
Since sonolysis process alone increases turbidity (Figure 4), a significant effect on
absorbance measurements was detected in sonolysis process as well as in combined
sonolysis-ozonation process (Figure 5). The turbidity increases as sonication time and
power densities increase. Moreover, the increased kinetic activity due to higher
temperatures results in higher turbidity compared to experiments carried out in controlled
temperature conditions for all investigated power densities as well as sonication times.
60
Turbidity (NTU)
50
25 W/L (TC)
100 W/L (TC)
200 W/L (TC)
25 W/L (NTC)
100 W/L (NTC)
200 W/L (NTC)
40
30
20
Initial turbidity
10
0
15
30
Sonication time (min)
Figure 4: effect of ultrasounds on turbidity with (TC) and without temperature control
(NTC), using different ultrasound densities, according to contact time.
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Ozonation process was effective for colour removal (Figure 5.a); the UV436 decreased
from 13 to 41% as contact time increased from 3 to 15 min. On the opposite, because of
turbidity interference the absorbance spectra increased as sonication time increased
(Figure 5.b). The efficiency of the combined process sonolysis/ozonation showed a less
UV436 decrease compared to ozonation process alone (Figure 5.c); in particular, only 3%
decrease after 15 min, and 40% was detected only after 60 min contact time.
0.2
0.18
0 min
3 min
5 min
7.5 min
10 min
15 min
UV absorbance (1/cm)
0.16
0.14
0.12
0.1
0.08
0.06
0.04
0.02
0
200
250
300
a)
350
400
450
wavelength (nm)
500
550
600
0.2
0.18
0 min
5 min
15 min
60 min
UV absorbance (1/cm)
0.16
0.14
0.12
0.1
0.08
0.06
0.04
0.02
0
200
250
300
b)
350
400
450
wavelength (nm)
500
550
600
0.2
UV absorbance (1/cm)
0.18
0 min
5 min
15 min
60 min
0.16
0.14
0.12
0.1
0.08
0.06
0.04
0.02
0
200
300
400
wavelength (nm)
500
600
c)
Figure 5: behaviour of UV spectra for ozonated (a), sonicated (b) and
ozonated/sonicated (c) wastewater.
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4.
CONCLUSIONS
In this paper, the use of ozonation and sonolysis as advanced treatment for the reuse of
domestic wastewater was investigated in terms of bacteria inactivation (E. coli) as well as
colour removal. The results can be summarized as follows:
1. according to the investigated conditions the ozonation process alone was not
enough to decrease the E. coli colonies under the limit set for wastewater reuse in
Italy (10 CFU/100 mL).
2. sonolysis improved ozonation process; after 15 min contact time E. coli colonies
decreased under 10 CFU/100 mL.
3. the effect of temperature in sonolysis experiments was significant. Under not
controlled temperature conditions, the total E. coli inactivation at 15 min contact
time was achieved using a power density of 200 W/L. Under controlled
temperature conditions the total inactivation was reached at 30 min using the
maximum power density (200 W/L).
4. ozonation process was effective for colour removal; the UV436 decreased from 13
to 41% as contact time increased from 3 to 15 min. On the opposite, because the
sonolysis process alone increased colour, not significant differences in terms of
UV436 were observed comparing ozonation and combined sonolysis/ozonation
process.
Thus the combined process ozonation-sonolysis has got the potential to be a valuable
alternative to conventional oxidation/disinfection processes when less expensive
solutions such as chlorination cannot be applied because of very stringent limits set by
regulations (e.g., trihalomethanes) as well as specific uses advise against to a given
oxidant/disinfectant. However, care must be take in setting up the more suitable operating
conditions in order to avoid an increase of colour because of sonolysis process.
ACKNOWLEDGEMENTS
The authors are grateful to Milena Landi for her technical support in sonolysis
experiments, Aeraque for ozone generator, Pluriacque (Salerno, Italy), particularly dr.
Antonella Anselmo and dr. Stefania Borrelli, for data in table 1.
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