Articles
Effect of handwashing on child health: a randomised
controlled trial
Stephen P Luby, Mubina Agboatwalla, Daniel R Feikin, John Painter, Ward Billhimer MS, Arshad Altaf, Robert M Hoekstra
Summary
Background More than 3·5 million children aged less than 5 years die from diarrhoea and acute lower respiratory-tract
infection every year. We undertook a randomised controlled trial to assess the effect of handwashing promotion with
soap on the incidence of acute respiratory infection, impetigo, and diarrhoea.
Methods In adjoining squatter settlements in Karachi, Pakistan, we randomly assigned 25 neighbourhoods to
handwashing promotion; 11 neighbourhoods (306 households) were randomised as controls. In neighbourhoods with
handwashing promotion, 300 households each were assigned to antibacterial soap containing 1·2% triclocarban and
to plain soap. Fieldworkers visited households weekly for 1 year to encourage handwashing by residents in soap
households and to record symptoms in all households. Primary study outcomes were diarrhoea, impetigo, and acute
respiratory-tract infections (ie, the number of new episodes of illness per person-weeks at risk). Pneumonia was
defined according to the WHO clinical case definition. Analysis was by intention to treat.
Findings Children younger than 5 years in households that received plain soap and handwashing promotion had a
50% lower incidence of pneumonia than controls (95% CI –65% to –34%). Also compared with controls, children
younger than 15 years in households with plain soap had a 53% lower incidence of diarrhoea (–65% to –41%) and a
34% lower incidence of impetigo (–52% to –16%). Incidence of disease did not differ significantly between households
given plain soap compared with those given antibacterial soap.
Interpretation Handwashing with soap prevents the two clinical syndromes that cause the largest number of childhood
deaths globally—namely, diarrhoea and acute lower respiratory infections. Handwashing with daily bathing also
prevents impetigo.
Introduction
Every year, more than 3·5 million children aged less
than 5 years die from diarrhoea and acute lower
respiratory-tract
infection.1 These
deaths
are
concentrated in low-income communities in developing
countries.2–4 Several studies have shown that regular
handwashing with soap reduces the incidence of
diarrhoea in children younger than 5 years in
communities with a high incidence of diarrhoea,5–7
although we are unaware of any reports of the effect of
handwashing on acute respiratory-tract infections in
settings where pneumonia is a leading cause of death.
In developed countries, the promotion of
handwashing has reduced respiratory-tract infections in
several settings. Controlled trials of handwashing
promotion in child-care centres have reported a 14%
reduction in upper respiratory-tract infection in
Canada;8 a 12% reduction in upper respiratory-tract
infection in children aged 24 months or less in
Australia;9 and a 32% reduction in colds in one US
child-care centre in the USA.10 In a school of children
aged 5 to 12 years in the USA, a handwashing
promotion programme in selected classrooms was
associated with a 21% fall in absences from respiratory
illness.11 Another programme at a US Navy training
centre that included directives to wash hands five times
per day resulted in a 45% reduction in total outpatient
visits for respiratory illness.12
www.thelancet.com Vol 366 July 16, 2005
Lancet 2005; 366: 225–33
See Comment page 185
Division of Bacterial and
Mycotic Diseases, National
Centers for Infectious Diseases,
Centers for Disease Control and
Prevention, Atlanta, GA 30333,
USA (S P Luby MD,
D R Feikin MD, J Painter DVM,
R M Hoekstra PhD); Health
Oriented Preventive Education,
Karachi, Pakistan
(M Agboatwalla MBBS);
Community Health Sciences,
Aga Khan University, Karachi,
Pakistan (A Altaf MBBS); and
The Procter and Gamble
Company, Cincinnati, OH, USA
(W Billhimer MS)
Correspondence to:
Dr Stephen P Luby
sluby@icddrb.org
Impetigo is another condition that is common in lowincome countries with high humidity, which affects
mothers of young children. A previous study13 in Karachi,
Pakistan, investigated the effect of antibacterial soap on
impetigo. Incidence of impetigo in children living in
households receiving antibacterial soap (1·10 episodes
per 100 person-weeks) was 23% lower than that in
households receiving plain soap (p=0·28) and was 43%
lower than the standard habit and practice controls
(p=0·02).
In Karachi, more than 4 million low-income residents
live in squatter settlements where they do not legally
own the land, and municipal infrastructure is
restricted.14 A study undertaken in these communities
concluded that 41% of deaths of children aged less than
5 years were due to diarrhoea and 15% due to acute
respiratory-tract infections.15
We undertook the Karachi Soap Health Study as a
randomised controlled trial to measure the broad health
benefits brought about by improvement of handwashing
and bathing with soap in settings where communicable
diseases are leading causes of childhood morbidity and
mortality.
Materials and methods
Patients
The study site and intervention for the Karachi Soap
Health Study has been described previously.16 Briefly, the
225
Articles
Community leaders and heads of households also
provided informed consent. Ill children were referred to
the appropriate health care. The study protocol was
approved by the ethics review committee of Aga Khan
University, and by an institutional review board of the
Centers for Disease Control and Prevention.
42 neighbourhoods identified in study area
36 neighbourhoods closest to study
centre enrolled and randomised
25 neighbourhoods assigned
to handwashing promotion
11 neighbourhoods
assigned to control
300 households assigned
to use antibacterial soap
300 households assigned
to use plain soap
306 control households
1523 children younger
than 15 years
enrolled at baseline
1640 children younger
than 15 years
enrolled at baseline
1528 children younger
than 15 years
enrolled at baseline
51 children born in
65 children aged out
1 child died
44 children born in
70 children aged out
3 children died
40 children born in
82 children aged out
3 children died
77 075 potential personweeks follow-up
82 890 potential personweeks follow-up
76 878 potential personweeks follow-up
67 630 (88%) actual personweeks follow-up
73 850 (89%) actual personweeks follow-up
68653 (89%) actual personweeks follow-up
504 children younger
than 5 years
(20 191 child-weeks)
assessed for pneumonia
517 children younger
than 5 years
(20 009 child-weeks)
assessed for pneumonia
489 children younger
than 5 years
(18 392 child-weeks)
assessed for pneumonia
Figure 1: Trial profile
study was undertaken in adjoining multi-ethnic squatter
settlements in central Karachi in collaboration
with Health Oriented Preventive Education (HOPE),
a non-governmental organisation that supports
community-based health and development initiatives.
Although handwashing, typically with water only, is
part of ritual preparation for prayer in these societies,
thorough washing of hands with soap is not as
common. Affordable hand soap is widely available
throughout
these
communities
from
small,
neighbourhood shops.
Fieldworkers identified 42 candidate neighbourhoods
separated by a street or market area. Eligible households
were located in a candidate neighbourhood, had at least
two children younger than 15 years (at least one of whom
was less than 5 years old), and were planning to continue
to reside in the same home for the duration of the study.
Households that received a soap or water-vessel
intervention in any previous study with HOPE were
excluded. Fieldworkers undertook a census of these
neighbourhoods, and before intervention assignment,
they identified and obtained informed consent from
1050 candidate households.
226
Procedures
Fieldworkers listed the candidate neighbourhoods in
the order of proximity to their field centre. One of the
investigators (SL) who did not participate in recruiting
neighbourhoods or households programmed a spreadsheet to randomly generate the integers of a 1 or a 2,
with a 2 being twice as likely to be generated than a 1.
He applied the random numbers sequentially to the list
of neighbourhoods. Neighbourhoods with a 1 were
assigned to control, and those with a 2 were assigned to
handwashing
promotion.
Random
assignment
continued until neighbourhoods consisted of at least
600 handwashing promotion households and
300 control households were assigned. Overall,
25 neighbourhoods were assigned to handwashing
promotion and 11 to control (figure 1). Handwashing
promotion was assigned at the neighbourhood level,
because several activities promoting handwashing were
undertaken at this level. Antibacterial versus plain soap
was randomly assigned at the household level.
During the week before soap was distributed and
disease surveillance began, fieldworkers gathered
participants from ten to 15 nearby homes for
neighbourhood meetings that lasted 30–45 min. They
showed slide shows, videotapes, and pamphlets to
illustrate health problems resulting from contaminated
hands and to provide specific handwashing
instructions. They encouraged discussions between
participants. For the first 2 months of the intervention,
neighbourhood meetings were held for the mothers in
every neighbourhood two or three times a week. These
were reduced to weekly meetings between 2 and
9 months, and to fortnightly meetings for the last
3 months. For the first 3 months, men also had monthly
meetings that covered the same information.
Fieldworkers visited intervention households at least
once a week to discuss the importance of handwashing
and correct handwashing technique, and to promote
regular handwashing habits. They encouraged
participants to wet their hands, lather them completely
with soap, rub them together for 45 s, and rinse the
lather off completely. Hands were typically dried on
participants’ clothing. Fieldworkers encouraged all
individuals in intervention households who were old
enough to understand (generally those aged more than
30 months) to wash their hands after defaecation, after
cleaning an infant who had defaecated, before
preparing food, before eating, and before feeding
infants. Fieldworkers also encouraged participants to
bathe once a day with soap and water. Initially,
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Articles
fieldworkers provided 2–4 bars of soap per household,
depending on size. Throughout the study, soap was
regularly replaced during use.
The Procter & Gamble company (Cincinnati, OH,
USA) manufactured all the soap for the study. The
antibacterial soap (Safeguard Bar Soap) contained 1·2%
triclocarban as an antibacterial substance. The plain
soap was identical to the antibacterial soap except that it
did not contain triclocarban. Both types were provided
as 90 g white bars without brand names or symbols.
They looked and smelled the same and were packaged
identically in generic white wrappers. Cases of 96 bars
were identified by serial numbers that were matched to
households. Neither the fieldworkers nor the families
knew whether soaps were antibacterial or plain.
Fieldworkers provided control families with a regular
supply of children’s books, notebooks, pens, and pencils
to help with their children’s education, but they gave no
products that would be expected to affect rates of
respiratory illness, diarrhoea, or impetigo. Fieldworkers
neither encouraged nor discouraged handwashing in
control households, and visited both control and
intervention households with equal frequency.
Fieldworkers were extensively trained in interviewing
techniques, data recording, approaches to promote
handwashing, weighing children, and clinical
assessment of ill children (including measurement of
respiratory rates). They were not trained to assess chestwall indrawing, because of the difficulty that trained
health-care workers have in identifying this symptom
reliably.17,18 The fieldworkers identified lesions
consistent with impetigo in children younger than
15 years old. A study physician then visited all
households to confirm the diagnosis of impetigo and
referred the family to locally available health-care
services.
The
same
fieldworkers
promoted
handwashing and obtained outcome data during their
weekly visits to intervention households. These 22
fieldworkers
rotated
between
neighbourhoods
(including both intervention and control) throughout
the study.
Trained fieldworkers undertook pre-intervention
baseline surveys, and identified children younger than
15 years. Children’s dates of birth were confirmed with
birth certificates or immunisation records. Fieldworkers
visited participating households at least once a week for
1 year (from April 15, 2002, to April 5, 2003) and asked
the mother (or other caregiver) whether the child had
any symptoms of cough or difficulty breathing in the
preceding week. They also asked whether the child had
any nasal congestion or coryza (runny nose). If a child
was symptomatic with cough or difficult breathing,
fieldworkers counted the number of breaths the child
took in 60 s timed with a watch.
The primary outcomes for our study were diarrhoea,
impetigo, and acute respiratory-tract infections (ie, the
number of new episodes of illness per person-weeks at
www.thelancet.com Vol 366 July 16, 2005
risk), although we specifically detail respiratory and
impetigo findings in this report. Results for diarrhoea
have been published elsewhere.16
We defined pneumonia in children according to the
WHO clinical case definition—cough or difficulty
breathing with a raised respiratory rate (⬎60 per min in
individuals younger than 60 days old, ⬎50 per min for
those aged 60–364 days, and ⬎40 per min for those
aged 1–5 years).19 We did not attempt to assess
pneumonia in children aged more than 5 years. An
episode of impetigo was defined as a new skin eruption,
which was confirmed by the study physician. Only after
the diagnosed episode was recorded to have cleared by
the community health worker (on one of the weekly
visits) could the study participant be a candidate for a
new episode of impetigo.
Fieldworkers weighed children aged less than 5 years
at baseline and every 4 months. They used a hanging
Salter scale for children younger than 3 years and a
bathroom scale for those older than 3 years. We
calculated weight-for-age Z scores compared with the
National Center for Health Statistics standards. Mean
weight-for-age Z scores were calculated from the
multiple weighing and measuring sessions throughout
the study for every child. We classified children as
moderately malnourished if their mean Z score was less
than –2·0 and –3·0 or greater, and severely malnourished if their score was less than –3·0.
Statistical analysis
Primary hypotheses of the Karachi Soap Health Study
were that the promotion of handwashing with
antibacterial or plain soap would significantly reduce
the frequency of diarrhoea and acute respiratory-tract
infection, and that bathing with antibacterial soap
would reduce the frequency of impetigo compared with
plain soap. Children who did not have the specific
syndrome in the preceding week but developed the
syndrome were regarded as having a new episode. Only
children who did not have the syndrome in the
preceding week were defined as at risk for a new
episode.
Individuals per household
Children less than 5 years old per household
Rooms in house
Bars of hand soap purchased in preceding 2 weeks
Households with infants
Children less than 5 years old with moderate malnutrition
Children less than 5 years old with severe malnutrition
Literacy of mother of youngest child in household
Monthly household income less than US$60
Households with natural gas used for cooking fuel
Antibacterial soap
(n=300)
Plain soap
(n=300)
Control
(n=306)
9·3 (4·1)
1·7 (0·5)
2·0 (0·9)
1·9 (0·7)
71 (24%)
125 (26%)
20 (4%)
113 (38%)
137 (46%)
285 (95%)
10·0 (4·8)
1·7 (0·5)
2·0 (1·0)
2·0 (0·7)
64 (21%)
103 (21%)
24 (5%)
107 (36%)
131 (44%)
285 (95%)
9·1 (4·1)
1·6 (0·4)
2·1 (0·9)
1·9 (0·7)
71 (23%)
114 (24%)
19 (4%)
98 (32%)
152 (50%)
298 (97%)
Data are mean (SD) or number (%).
Table 1: Baseline household characteristics by intervention group
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Antibacterial soap
Plain soap
Control
Cough or difficulty breathing in children
younger than 15 years (n=186 654)
Congestion or coryza in children
younger than 15 years (n=174 483)
Pneumonia in children
younger than 5 years (n=58 592)
Mean incidence*
Difference vs control
(95% CI)
Mean incidence*
Difference vs control (95% CI)
Mean incidence*
Difference vs control (95% CI)
4·21
4·16
8·50
–50% (–65% to –36%)
–51% (–65% to –37%)
··
7·32
6·87
14·78
–51% (–62% to –39%)
–54% (–63% to –44%)
··
2·42
2·20
4·40
–45% (–64% to –26%)
–50% (–65% to –34%)
··
*Incidence (of new episodes per 100 person-weeks) calculated with mean of every cluster incidence weighted by person-weeks at risk for that cluster.
Table 2: Primary respiratory outcomes by intervention group
Diarrhoea incidence (n=197 049)
Impetigo incidence (n=200 156)
Mean
Difference vs
incidence* control (95% CI)
Mean
Difference vs control
Difference vs plainincidence* neighbourhoods (95% CI) soap households
(95% CI)†
Antibacterial soap 2·02
Plain soap
1·91
Control
4·06
–50% (–64% to –37%)
–53% (–65% to –41%)
··
0·61
0·62
0·94
–36% (–53% to –18%)
–34% (–52% to –16%)
··
–2% (–24% to 20%)
··
··
*Mean incidence (episodes per 100 person-weeks) calculated with mean of neighbourhood rates weighted by person-weeks at
risk from every neighbourhood. †Accounts for clustering by household.
Table 3: Primary diarrhoea and impetigo outcomes in children younger than 15 years by intervention
group
We calculated a sample size of 220 households per
intervention group, assuming the same rates of impetigo
as those recorded in the previous Karachi study13 (ie, 2·07
episodes of impetigo per 100 person-weeks in the control
group, 1·58 episodes per 100 person-weeks in the plainsoap group, and 1·10 episodes per 100 person-weeks in
the antibacterial-soap group). We assumed a mean of 3·8
children per household, 95% follow-up, 10% dropout,
and a doubling of sample size to offset the effect of
clustering by neighbourhood and repeated measures. We
increased the sample size to 300 households per group to
provide additional power for subgroup analysis, and to
protect against inherent uncertainties in the sample-size
assumptions.
Because we assigned soap promotion versus control at
the neighbourhood level, we also analysed primary
health outcomes at this level. Specifically, within every
neighbourhood, we identified the total number of new
episodes of illness (in person-weeks in the subgroups of
interest) and divided it by the total number of personweeks at risk for children in that neighbourhood within
the subgroup of interest. Incidence rates by intervention
assignment (the mean of appropriate neighbourhood
incidence rates) were weighted by the number of personweeks at risk from all neighbourhoods that contributed
to the mean. We calculated rate ratios by dividing the
mean incidence from intervention neighbourhoods by
that from control neighbourhoods. Because we
compared antibacterial soap with plain soap at the
household level, we also analysed the difference in
impetigo incidence between antibacterial and plain soap
at this level.
We calculated 95% CIs of rate ratios using Taylor
series approximations to obtain standard errors. The
difference (%) in outcome between intervention and
10
Control
Antibacterial soap
Plain soap
Episodes per 100 person-weeks
8
6
4
2
M
ar
ch
ry
ua
br
Fe
ry
ua
Jan
be
ce
m
De
No
v
em
be
r
r
r
be
to
Oc
be
te
m
Se
p
gu
Au
2002
r
st
y
Ju
l
ne
Ju
ay
M
Ap
ril
0
2003
Figure 2: Incidence of pneumonia in children younger than 5 years
228
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control was reported (ie, rate ratio minus one). We
regarded differences (%) between groups as significant
if 95% CIs excluded 0%.
After the 1 year of planned data collection was
completed, Procter & Gamble provided the list
identifying which cases of soap were antibacterial or
plain for analysis. Disease experiences of every child,
household, and neighbourhood was tracked and
analysed with the group they were originally assigned
to (intention-to-treat analysis).
Role of the funding source
One author (WB) was an employee of the study sponsor
(Procter & Gamble). He reviewed and provided
feedback on the study protocol, analysis, and
manuscript. The corresponding author had full access
to all the data in the study and had final responsibility
for the decision to submit for publication. The Centers
for Disease Control and Prevention retained the right
to decide what to submit for publication and to publish
results without approval from Procter and Gamble.
Results
The 36 neighbourhoods in the study had a median of
26 participating households per neighbourhood
(IQR 21–30). In the 25 neighbourhoods randomly
assigned to handwashing promotion, antibacterial soap
was randomly allocated to 300 households and plain
soap to 300 households. 11 neighbourhoods (306 households) were randomly assigned to undertake standard
habits and practices (control). During 51 weeks of
follow-up, information was obtained on 210 133 personweeks, representing 89% of the study populations’
experience. Follow-up was similar in the three
intervention groups (figure 1). The most common
reason for missing follow-up information was that the
family had temporarily left town to visit relatives.
At baseline, households in the three intervention
groups were similar (table 1). Households had a mean
of 9·5 residents (SD 4·4) and purchased about one bar
of hand soap per week. During the study, households
assigned to handwashing promotion received a mean
of 3·3 bars of study soap per week; thus, every
household resident used an average of 4·4 g of soap per
day.
Neighbourhoods that received soap and encouragement to wash their hands and bathe had
substantially less respiratory disease, impetigo, and
diarrhoea than controls (tables 2 and 3; diarrhoea data
also reported elsewhere).16 Children younger than
15 years who lived in households that received plain
soap in neighbourhoods where handwashing was
promoted, had more than a 50% lower incidence of
cough or difficult breathing and of congestion or coryza
than did children in control neighbourhoods. Children
aged less than 5 years who received plain soap in
neighbourhoods where handwashing was promoted had
a 50% lower incidence of pneumonia than did children
in control neighbourhoods. Incidence of acute
respiratory illness in households receiving antibacterial
soap was much the same as that in households receiving
plain soap (table 2).
Incidence of impetigo was 36% lower in children in
households that received antibacterial soap and
encouragement to bathe every day than in those in
control neighbourhoods but only 2% lower than in
children in plain-soap households (table 3). Mean
duration of impetigo episodes was 18 days (SD 14) for
children in antibacterial-soap households, 18 days (13)
for those in plain-soap households, and 22 days (15) for
20
Control
Antibacterial soap
Plain soap
Episodes per 100 person-weeks
15
10
5
2002
ch
M
ar
ry
ua
br
Fe
ua
ry
Jan
r
m
be
De
ce
m
be
r
ve
No
Se
Oc
to
be
r
r
em
be
gu
Au
pt
st
ly
Ju
ne
Ju
ay
M
Ap
ril
0
2003
Figure 3: Incidence of cough and difficult breathing in children younger than 15 years
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229
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40
Control
Antibacterial soap
Plain soap
35
Episodes per 100 person-weeks
30
25
20
15
10
5
ch
ar
M
Fe
br
ua
ry
ry
Jan
m
ce
De
ua
r
be
r
be
m
ve
No
Oc
to
be
r
r
em
Se
pt
gu
Au
be
st
ly
Ju
Ju
ne
ay
M
Ap
ril
0
2003
2002
Figure 4: Incidence of congestion and coryza in children younger than 15 years
those in control households. Weekly prevalence of
impetigo among children in households that received
antibacterial soap and encouragement to bathe daily was
47% lower (–61% to –34%) than those in control
neighbourhoods, but only 4% lower than children living
in plain-soap households (–28% to 19%).
For the first 6 months of the trial, incidence of
pneumonia in children younger than 5 years who lived
in neighbourhoods receiving handwashing promotion
with soap was only 6% lower (–43% to 32%) than in
children in control neighbourhoods (figure 2). However,
in the second 6 months, when pneumonia was much
more frequent in the control group, incidence of
pneumonia was 66% lower (–79% to –53%; figure 2). A
similar pattern of illness rates was noted in the
incidence of cough or difficulty breathing and
congestion or coryza in all ages of participants (figures 3
and 4).
Compared with children aged 2–5 years, children aged
less than 1 year had a 1·4 times greater incidence of
pneumonia (2·8 vs 2·1 episodes per 100 person-weeks)
and children aged 1–2 years had a 3·5 times higher
incidence of pneumonia (7·2 vs 2·1 episodes per 100
person-weeks). There was less difference in pneumonia
incidence in handwashing-promotion versus control
neighbourhoods in children younger than 1 year than
the difference seen in children up to the age of 5 years
(table 4). Pneumonia incidence in children who received
plain soap was nearly 30% lower in those younger than
1 year, more than 50% lower in those aged 1–2 years,
and just over 50% lower in those aged 2–5 years,
compared with controls. Age-specific incidence of
pneumonia was closely similar between households
receiving antibacterial versus plain soap (table 4). When
data for households that received both types of soap were
combined, children younger than 1 year had a 27%
lower incidence of pneumonia (–77% to 24%), a 36%
lower incidence of cough and difficulty breathing (–59%
to –12%), and a 39% lower incidence of congestion and
coryza (–56% to –22%) than controls.
Moderately malnourished children had the same risk
of pneumonia as non-malnourished children (2·9 vs
2·8 episodes per 100 person-weeks); but severely malnourished children had 1·4 times the incidence of
pneumonia of non-malnourished children (4·2 vs
2·9 episodes per 100 person-weeks, p=0·003).
Individuals aged less than 1 year (n=7679)
Individuals aged 1–2 years (n=9238)
Individuals aged 2–5 years (n=41 675)
Pneumonia
incidence*
Difference vs
control (95% CI)
Pneumonia
incidence*
Difference vs
control (95% CI)
Pneumonia
incidence*
–24% (–80% to 32%)
–29% (–78% to 20%)
··
5·93
5·04
10·86
–45% (–69% to –21%) 1·55
–54% (–74% to –33%) 1·56
··
3·17
Antibacterial soap 2·65
Plain soap
2·46
Control
3·48
Difference vs
control (95% CI)
–51% (–70% to –33%)
–51% (–68% to –34%)
··
*Incidence (episodes per 100 person-weeks) calculated with mean incidence of every cluster weighted by person-weeks at risk for that cluster.
Table 4: Mean pneumonia incidence by age and intervention group
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Nutrition status
(person-weeks at risk)*
Antibacterial soap
Plain soap
Control
No malnutrition (n=38 830)
Moderate malnutrition (n=13 604)
Severe malnutrition (n=3591)
Pneumonia
incidence†
Difference
vs control (95% CI)
Pneumonia
incidence†
Difference
vs control (95% CI)
Pneumonia
incidence†
Difference
vs control (95% CI)
2·42
2·12
4·07
–40% (–61% to –20%)
–48% (–65% to –31%)
··
1·83
2·24
4·91
–63% (–80% to –46%)
–54% (–75% to –34%)
··
4·18
3·08
5·73
–27% (–60% to 6%)
–46% (–74% to –18%)
··
*Total number of person-weeks at risk is lower than total in table 3, because of missing anthropometry data for 96 children. †Incidence (episodes per 100 person-weeks) calculated with
mean incidence of every cluster weighted by person-weeks at risk for that cluster.
Table 5: Mean pneumonia incidence in children younger than 5 years by nutrition status and intervention group
Handwashing with soap was effective against
pneumonia in well-nourished and malnourished
children (table 5). Compared with controls, pneumonia
incidence in households that received plain soap was
roughly halved in children irrespective to who was nonmalnourished, moderately malnourished, and severely
malnourished. Households using antibacterial soap had
rates of pneumonia comparable to rates for plain soap,
although the fall in rate was not significant in children
with severe malnutrition living in households receiving
antibacterial soap (table 5).
Discussion
In squatter settlements of Karachi, where diarrhoea and
acute respiratory infections are leading causes of death,
washing hands with soap reduced both syndromes by
half. Incidence of impetigo also fell by almost a third in
households encouraged to regularly wash hands and
bathe daily. Handwashing was effective in reducing
disease incidence in malnourished children, although
the reductions in the youngest children were not
significant. However, we did not detect any difference in
disease incidence between the use of antibacterial and
plain soap.
The reduction in incidence of disease was almost
certainly due to handwashing with soap only, because
interventions were randomly assigned to neighbourhoods, and the participating households had similar
characteristics known to affect the incidence of diarrhoea
and respiratory disease. Indeed, incidence of respiratory
disease did not vary between neighbourhoods for the
first several months of the study. The trebling of soap
consumption in handwashing promotion households
from baseline suggests that handwashing was more
regularly practised. Moreover, although this study
specifically shows a fall in pneumonia frequency from
handwashing, the finding is consistent with data in other
settings that have suggested a reduction in respiratory
disease from handwashing with soap.8–12
These findings are biologically plausible. At least some
of the viruses that usually infect the respiratory tract are
readily transmitted from person to person by hand
contact.20,21 In developing countries, viruses commonly
cause pneumonia.22,23 Additionally, several viruses that
infect the human respiratory tract predispose children to
bacterial pneumonia.24–26
www.thelancet.com Vol 366 July 16, 2005
Soap and the mechanical activity of rubbing hands
together enhances the capacity of water to solubilise and
to remove dirt and pathogens from hands.27,28 This
physical removal of pathogens reduces the efficiency of
not only pathogen transmission from the hands of an
infected person to those of an uninfected person, but also
the transmission of infectious organisms from the hands
of an uninfected person to the respiratory tract.
Triclocarban is effective in vitro against some species of
Streptococcus, but does not have substantial activity
against gram-negative bacteria or viruses.29,30 The closely
similar effectiveness of antibacterial and plain soap
suggests that the physical removal of pathogens from
hands and skin with soap and water, rather than the
specific antibacterial activity of triclocarban, is the key
factor in the prevention of diarrhoea, impetigo, and
respiratory infections.
Siblings and other children transmit most respiratory
pathogens to young children.31–33 Handwashing that
interrupts transmission of respiratory pathogens in older
siblings and other neighbourhood children not only
reduces their own rate of respiratory illness, but also
shrinks the pool of people who are transmitting and thus
restricts exposure in vulnerable children.
In our study, respiratory infections did not decrease
immediately after the introduction of handwashing
promotion, but fell substantially after several months.
The changing of handwashing habits takes time34 and
could need several months for the habit to be taken up by
enough family members to interrupt respiratory
pathogen transmission. Furthermore, respiratory
disease is seasonal in Pakistan; typically more than twice
as many cases take place from October to March
compared with April to September.35,36 Children living in
handwashing promotion neighbourhoods avoided the
typical winter peak in respiratory disease, suggesting that
the transmission of organisms responsible for the winter
peak is efficiently interrupted by regular handwashing.
Most childhood respiratory-tract infections are mild
and do not lead to childhood death. It is less clear from
our data if many severe, life-threatening, lower
respiratory-tract infections were prevented. Fieldworkers
used the WHO case definition (integrated management
of childhood illness [IMCI]) for pneumonia to identify
children with lower respiratory-tract infections. They
were not trained to recognise lower chest-wall indrawing
231
Articles
or other signs of severe pneumonia that are highly
associated with mortality. In clinics, pneumonia is
typically over-diagnosed with the IMCI case definition.
For example, of 965 Kenyan children who were
classified as meeting the IMCI case definition for
pneumonia, 38% were judged not to have the disease
based on chest radiography and physician diagnosis.37
We would expect the IMCI case definition to identify an
increased proportion of false-positive diagnoses of
pneumonia in communities, because risk of disease is
lower in these individuals than in clinic attendees.
However, the study results suggest that handwashing
with soap prevents at least some cases of severe
pneumonia. First, some mild upper respiratory-tract
infections in children and in their siblings predispose to
severe lower respiratory-tract infection.24–26,38 Thus,
prevention of the mild, upper respiratory-tract
infections can also stop severe pneumonia. Second, in
the study, handwashing with soap was effective against
pneumonia in some children at high risk for death from
pneumonia, such as those with severe malnutrition.39–41
Incidence of pneumonia was roughly halved in these
children if they lived in a household in which
handwashing with plain soap was promoted. Of the
youngest children—ie, those aged less than 1 year who
make up most deaths from acute respiratory infection—
handwashing with soap had less of an effect. The
reduction in pneumonia (close to 30%) was not
significant in these young children from households
with handwashing promotion. This non-significance
probably indicated restricted statistical power. Indeed,
for the other respiratory outcomes that occur more
frequently than pneumonia, the reduction in incidence
in infants was significant. Infants were not washing
their own hands, nor were families encouraged to wash
infants’ hands. Because the removal of pathogens from
older people is the only route of transmission to infants
that handwashing would interrupt, a more moderate
reduction in disease in infants than in older children is
expected. Ultimately, the proportion of severe respiratory illness that is prevented with handwashing
needs to be established.
These data, consistent with those of other
investigations,13,42 suggest that daily bathing with soap
lowered the incidence of childhood impetigo. The study
showed no added benefit of including triclocarban in
soap. This finding differs from previous data13 from
Karachi suggesting that children in households with
tricloclarban-containing soap had a 23% lower
incidence of impetigo compared with those in
households receiving placebo soap. However, the
difference in the earlier study was not significant
(p=0·28). Our present study enrolled nearly four times
as many households, and was intentionally designed
with sufficient power to detect a 23% difference
between triclocaraban-containing and placebo soap
types. The absence of an effect in a randomised,
232
placebo-controlled trial that was masked and sufficiently
powered, suggests that the addition of triclocarban to
soap does not prevent impetigo in this setting and that
the 23% difference in the earlier study was due to the
chance selection of small, non-representative study
groups. Indeed, antibacterial soap did not provide a
health advantage over plain soap for any of the health
outcomes in our study. Soap companies should
consider whether the funds and marketing efforts spent
in the addition of antibacterial compounds to soap
would be better deployed to improve the lathering and
sensory experience of handwashing to make the action
more pleasant and to actively promote regular handwashing.
Study personnel and participants were not masked to
the intervention. Thus, study participants in the
handwashing promotion groups, grateful for the soap,
could have kept reported episodes of illness in the
household to a minimum, as could have fieldworkers
recorded fewer episodes because of a desire, conscious
or not, to meet the expectation of the study sponsors.
However, fieldworkers were formally trained, and the
importance of accurate recording of reported symptoms
episodes was stressed. Supervisors, who regularly made
unannounced households visits, identified symptoms
that were different to those reported by fieldworkers in
less than 1% of visits.16
Our data show that regular handwashing with soap is
very effective in preventing diarrhoea16 and respiratory
disease, two of the leading causes of global childhood
death. Handwashing with daily bathing also prevents
impetigo. Provision of free soap, frequent community
meetings, and weekly household handwashing
promotion visits to all impoverished households worldwide is prohibitively expensive. Thus, the challenge for
the public-health community is to identify cost-effective
techniques for handwashing promotion that can reach
the hundreds of millions of households at risk.
Contributors
S Luby developed the idea for the study, drafted the study protocol,
analysed the data and drafted the manuscript. M Agboatwalla assisted in
development of the protocol, designed the handwashing promotion
intervention, supervised field implementation of the protocol, assisted in
interpretation of the data, and helped revise the manuscript. D Feikin
assisted in developing the assessment for acute respiratory-tract
infections, and provided published work from previous studies and
critical input to place these findings within the broader context of
respiratory diseases. J Painter designed the data management strategy,
developed the database for data entry and verification, assisted in
analysis, interpretation, and revision of the manuscript. A Altaf assisted
in developing the protocol addressing human subject issues, assisted in
project administration, and reviewed the manuscript. W Billhimer
critically reviewed the study protocol, analysis, and manuscript.
R M Hoekstra helped develop the analytic strategy, assisted in analysing
the data, and had the final say on appropriateness of analytic methods.
Conflict of interest statement
S Luby was supported by the grant from the Procter & Gamble company
that funded this study. W Billhimer is an employee of the Procter &
Gamble company. The other authors declare that they have no conflict of
interest.
www.thelancet.com Vol 366 July 16, 2005
Articles
Acknowledgments
The authors thank the HOPE staff workers who worked in the
community, Aqil Hussain, Gharnata Tabassum, Zahida Kanum,
Badar and Sabeen who supervised the daily field work and data
collection, and Faisal Sarwari who supervised data entry. Most of the
funding for this study was provided by Procter and Gamble,
manufacturer of Safeguard Bar Soap. The balance of the funding was
provided by the Centers for Disease Control and Prevention. Inclusion of
soap trade names is for identification only and does not imply
endorsement by CDC or the Department of Health and Human Services.
These data were presented in part at the International Conference on
Emerging Infectious Diseases, Atlanta, GA, USA, in February, 2004.
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