Academia.edu no longer supports Internet Explorer.
To browse Academia.edu and the wider internet faster and more securely, please take a few seconds to upgrade your browser.
2018, Genes, Genetics and Transgenics for Virus Resistance in Plants
Biotecnología …, 2009
Journal of Experimental Botany, 1997
Acta horticulturae
International Journal of Molecular Sciences
Plant viruses are the main pathogens which cause significant quality and yield losses in tomato crops. The important viruses that infect tomatoes worldwide belong to five genera: Begomovirus, Orthotospovirus, Tobamovirus, Potyvirus, and Crinivirus. Tomato resistance genes against viruses, including Ty gene resistance against begomoviruses, Sw gene resistance against orthotospoviruses, Tm gene resistance against tobamoviruses, and Pot 1 gene resistance against potyviruses, have been identified from wild germplasm and introduced into cultivated cultivars via hybrid breeding. However, these resistance genes mainly exhibit qualitative resistance mediated by single genes, which cannot protect against virus mutations, recombination, mixed-infection, or emerging viruses, thus posing a great challenge to tomato antiviral breeding. Based on the epidemic characteristics of tomato viruses, we propose that future studies on tomato virus resistance breeding should focus on rapidly, safely, and e...
Phytopathology, 2005
Tomato hybrids obtained from homozygous progeny of line 30-4, engineered for Tomato spotted wilt virus (TSWV) resistance, were tested under field conditions in two locations with their corresponding nontransgenic hybrids. No transgenic hybrid became infected, but 33 to 50% of plants of each nontransgenic hybrid became infected with a severe reduction of marketable fruit production. The transgenic hybrids conformed to the standard agronomic characteristics of the corresponding nontransgenic ones. Fruit were collected from the nontransgenic plots included in the experimental field and from border rows, and seed were used to estimate the flow of the transgene via pollen. No transgene flow was detected in the protected crops; however, in the open field experiment, 0.32% of tomato seedlings were found to contain the genetic modification. Immunity to TSWV infection in 30-4 hybrids was confirmed in laboratory conditions using mechanical inoculation and grafting. Thrips inoculation in leaf ...
Plants, 2020
Grafting is routinely implemented in modern agriculture to manage soilborne pathogens such as fungi, oomycetes, bacteria, and viruses of solanaceous crops in a sustainable and environmentally friendly approach. Some rootstock/scion combinations use specific genetic resistance mechanisms to impact also some foliar and airborne pathogens, including arthropod or contact-transmitted viruses. These approaches resulted in poor efficiency in the management of plant viruses with superior virulence such as the strains of tomato spotted wilt virus breaking the Sw5 resistance, strains of cucumber mosaic virus carrying necrogenic satellite RNAs, and necrogenic strains of potato virus Y. Three different studies from our lab documented that suitable levels of resistance/tolerance can be obtained by grafting commercial tomato varieties onto the tomato ecotype Manduria (Ma) rescued in the framework of an Apulian (southern Italy) regional program on biodiversity. Here we review the main approaches, ...
Jarq-japan Agricultural Research Quarterly, 1993
Molecular Breeding, 1999
For the production of broad commercial resistance to cucumber mosaic virus (CMV) infection, tomato plants were transformed with a combination of two coat protein (CP) genes, representing both subgroups of CMV. The CP genes were cloned from the CMV-D strain and Italian CMV isolates (CMV-22 of subgroup I and CMV-PG of subgroup II) which have been shown to produce severe disease symptoms. Four plant transformation vectors were constructed: pMON18774 and pMON18775 (CMV-D CP), pMON18831 (CMV-PG CP) and pMON18833 (CMV-22 CP and CMV-PG CP). Transformed R0 plants were produced and lines were selected based on the combination of three traits: CMV CP expression at the R0 stage, resistance to CMV (subgroup I and/or II) infection in growth chamber tests in R1 expressing plants, and single transgene copy, based on R1 segregation. The results indicate that all four vector constructs generated plants with extremely high resistant to CMV infection. The single and double gene vector construct produced plants with broad resistance against strains of CMV from both subgroups I and II at high frequency. The engineered resistance is of practical value and will be applied for major Italian tomato varieties.
O Intercultural Migrante: Teorias & Análises, 2023
A presente obra propõe um quadro teórico-analítico atualizado, que considera tanto os aspectos materiais do fenômeno como os fatores simbólicos e subjetivos que promovem e sustentam os fluxos migratórios transnacionais contemporâneos. Partindo de uma perspectiva interdisciplinar, que transita entre as diferentes matrizes teóricas e epistemológicas que colaboraram na formatação do campo dos Estudos Migratórios, o percurso reflexivo aqui desenvolvido, ao mesmo tempo que tenta contemplar todos os níveis da problemática, adota a Comunicação Intercultural como eixo central e linha condutora conceitual. A primeira parte do livro (Bases Teóricas) é composta por uma revisão teórico-metodológica, baseada numa ampla bibliografia dedicada à temática e marcada por sua pertinência, atualidade, abrangência e diversidade disciplinar. Além de uma releitura crítica das principais teorias e metodologias voltadas ou relacionadas à questão migratória transnacional e seus correlatos comunitários, diaspóricos, identitários e culturais, a análise aqui desenvolvida busca identificar e destacar o princípio ativo da Comunicação Intercultural no processo de negociação da condição migratória e diaspórica na contemporaneidade. Já a segunda parte (Projeções Analíticas) é composta por uma seleção de cinco análises tópicas. Antes de serem aplicações diretas das teorias aqui revisadas e dos quadros epistemológicos por nós elaborados, os textos escolhidos convergem em sua abordagem guiada pelos princípios metodológicos da Comunicação Intercultural e ilustram as possibilidades de conjugar, no mesmo edifício conceitual, os níveis materiais, simbólicos, sociais, culturais, políticos, econômicos e tecnológicos constitutivos do fenômeno migratório transnacional contemporâneo.
Gate Designs - تصميم البوابة الخارجية, 2023
Al-Madan Online N.º 27, Tomo 2, 2024
Modern Asian Studies, 2022
NIBR-rapport, 2008
Märsche der Moderne. Varianten eines globalen Phänomens, 2024
Chromatographia, 2000
Kybernetika, 1996
WIREs Water, 2018
Intelligent Data Analysis, 2016
JAMA network open, 2021
American Journal of Community Psychology, 2010
BMC complementary medicine and therapies, 2024