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PurposeMammalian expression of Cre recombinase driven by the human cytomegalovirus IE promoter
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11916 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonena
- Backbone size w/o insert (bp) 6000
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Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecre
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Speciesbacteriophage P1
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Insert Size (bp)1100
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Entrez Genecre (a.k.a. P1_gp003)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pBS185 carries the wt cre gene under the control of the major immediate early promoter and enhancer of human cytomegalovirus (hCMV). This is a strong promoter that gives fairly generalized expression. However, it is not a strong promoter in embryonic stem (ES) cells. The construct also carries the mouse metallothionein MT-I region including the polyadenylation site and several introns. For higher level expression in general and for better expression in ES cells plasmid pBS513 is superior. The sequence shown includes cre and the metallothionein region.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS185 CMV-Cre was a gift from Brian Sauer (Addgene plasmid # 11916 ; http://n2t.net/addgene:11916 ; RRID:Addgene_11916) -
For your References section:
Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase. Sauer B, Henderson N. New Biol. 1990 May . 2(5):441-9. PubMed 2288914
Map uploaded by the depositor.
